Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02525

Experiment: D-Maltose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02520 and BWI76_RS02525 are separated by 10 nucleotidesBWI76_RS02525 and BWI76_RS02530 overlap by 8 nucleotides BWI76_RS02520: BWI76_RS02520 - N-acetylmuramoyl-L-alanine amidase, at 515,678 to 517,015 _RS02520 BWI76_RS02525: BWI76_RS02525 - DNA mismatch repair protein MutL, at 517,026 to 518,921 _RS02525 BWI76_RS02530: BWI76_RS02530 - tRNA dimethylallyltransferase, at 518,914 to 519,864 _RS02530 Position (kb) 517 518 519Strain fitness (log2 ratio) -2 -1 0 1at 516.171 kb on - strand, within BWI76_RS02520at 516.196 kb on + strand, within BWI76_RS02520at 516.204 kb on - strand, within BWI76_RS02520at 516.367 kb on + strand, within BWI76_RS02520at 516.573 kb on + strand, within BWI76_RS02520at 516.610 kb on - strand, within BWI76_RS02520at 516.702 kb on + strand, within BWI76_RS02520at 516.739 kb on + strand, within BWI76_RS02520at 516.810 kb on + strand, within BWI76_RS02520at 517.279 kb on + strand, within BWI76_RS02525at 517.279 kb on + strand, within BWI76_RS02525at 517.287 kb on - strand, within BWI76_RS02525at 517.317 kb on + strand, within BWI76_RS02525at 517.317 kb on + strand, within BWI76_RS02525at 517.325 kb on - strand, within BWI76_RS02525at 517.358 kb on - strand, within BWI76_RS02525at 517.464 kb on - strand, within BWI76_RS02525at 517.790 kb on + strand, within BWI76_RS02525at 517.893 kb on - strand, within BWI76_RS02525at 517.926 kb on - strand, within BWI76_RS02525at 518.074 kb on - strand, within BWI76_RS02525at 518.152 kb on + strand, within BWI76_RS02525at 518.287 kb on + strand, within BWI76_RS02525at 518.325 kb on + strand, within BWI76_RS02525at 518.468 kb on - strand, within BWI76_RS02525at 518.502 kb on + strand, within BWI76_RS02525at 518.512 kb on + strand, within BWI76_RS02525at 518.621 kb on - strand, within BWI76_RS02525at 518.651 kb on + strand, within BWI76_RS02525at 518.652 kb on - strand, within BWI76_RS02525at 518.659 kb on - strand, within BWI76_RS02525at 518.758 kb on + strandat 518.816 kb on + strandat 518.846 kb on + strandat 518.854 kb on - strandat 518.854 kb on - strandat 518.940 kb on + strandat 519.180 kb on + strand, within BWI76_RS02530at 519.287 kb on - strand, within BWI76_RS02530at 519.287 kb on - strand, within BWI76_RS02530at 519.305 kb on - strand, within BWI76_RS02530at 519.530 kb on + strand, within BWI76_RS02530at 519.776 kb on + strandat 519.853 kb on + strandat 519.882 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Maltose (C)
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516,171 - BWI76_RS02520 0.37 +0.5
516,196 + BWI76_RS02520 0.39 -0.2
516,204 - BWI76_RS02520 0.39 +0.7
516,367 + BWI76_RS02520 0.51 -0.1
516,573 + BWI76_RS02520 0.67 -0.1
516,610 - BWI76_RS02520 0.70 +0.1
516,702 + BWI76_RS02520 0.77 -0.2
516,739 + BWI76_RS02520 0.79 -1.1
516,810 + BWI76_RS02520 0.85 +0.6
517,279 + BWI76_RS02525 0.13 +0.1
517,279 + BWI76_RS02525 0.13 -0.2
517,287 - BWI76_RS02525 0.14 -0.6
517,317 + BWI76_RS02525 0.15 +0.3
517,317 + BWI76_RS02525 0.15 +0.6
517,325 - BWI76_RS02525 0.16 +0.4
517,358 - BWI76_RS02525 0.18 +0.9
517,464 - BWI76_RS02525 0.23 -0.1
517,790 + BWI76_RS02525 0.40 +0.2
517,893 - BWI76_RS02525 0.46 +1.7
517,926 - BWI76_RS02525 0.47 +0.0
518,074 - BWI76_RS02525 0.55 +0.5
518,152 + BWI76_RS02525 0.59 +0.4
518,287 + BWI76_RS02525 0.67 +0.8
518,325 + BWI76_RS02525 0.69 -0.0
518,468 - BWI76_RS02525 0.76 +0.6
518,502 + BWI76_RS02525 0.78 +0.9
518,512 + BWI76_RS02525 0.78 +0.5
518,621 - BWI76_RS02525 0.84 +0.4
518,651 + BWI76_RS02525 0.86 +1.1
518,652 - BWI76_RS02525 0.86 +0.9
518,659 - BWI76_RS02525 0.86 -0.3
518,758 + -0.3
518,816 + +1.0
518,846 + +0.5
518,854 - +0.3
518,854 - -1.1
518,940 + -0.7
519,180 + BWI76_RS02530 0.28 +0.3
519,287 - BWI76_RS02530 0.39 -1.5
519,287 - BWI76_RS02530 0.39 -1.0
519,305 - BWI76_RS02530 0.41 -2.1
519,530 + BWI76_RS02530 0.65 -2.6
519,776 + -1.5
519,853 + +0.3
519,882 + +0.3

Or see this region's nucleotide sequence