Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS01245

Experiment: D-Maltose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS01240 and BWI76_RS01245 are separated by 67 nucleotidesBWI76_RS01245 and BWI76_RS01250 are separated by 61 nucleotidesBWI76_RS01250 and BWI76_RS01255 are separated by 246 nucleotides BWI76_RS01240: BWI76_RS01240 - phospholipase A, at 253,655 to 254,527 _RS01240 BWI76_RS01245: BWI76_RS01245 - DNA helicase RecQ, at 254,595 to 256,421 _RS01245 BWI76_RS01250: BWI76_RS01250 - threonine export protein RhtC, at 256,483 to 257,106 _RS01250 BWI76_RS01255: BWI76_RS01255 - homoserine/homoserine lactone efflux protein, at 257,353 to 257,973 _RS01255 Position (kb) 254 255 256 257Strain fitness (log2 ratio) -1 0 1at 253.615 kb on - strandat 253.657 kb on + strandat 253.664 kb on + strandat 253.664 kb on + strandat 253.672 kb on - strandat 253.672 kb on - strandat 253.743 kb on + strand, within BWI76_RS01240at 253.815 kb on + strand, within BWI76_RS01240at 253.823 kb on - strand, within BWI76_RS01240at 253.939 kb on - strand, within BWI76_RS01240at 254.025 kb on - strand, within BWI76_RS01240at 254.123 kb on + strand, within BWI76_RS01240at 254.316 kb on + strand, within BWI76_RS01240at 254.331 kb on + strand, within BWI76_RS01240at 254.339 kb on - strand, within BWI76_RS01240at 254.427 kb on + strand, within BWI76_RS01240at 254.437 kb on + strand, within BWI76_RS01240at 254.639 kb on + strandat 254.703 kb on + strandat 254.722 kb on + strandat 254.722 kb on + strandat 254.769 kb on - strandat 254.831 kb on + strand, within BWI76_RS01245at 254.901 kb on - strand, within BWI76_RS01245at 255.222 kb on - strand, within BWI76_RS01245at 255.654 kb on - strand, within BWI76_RS01245at 255.695 kb on + strand, within BWI76_RS01245at 255.739 kb on + strand, within BWI76_RS01245at 255.885 kb on + strand, within BWI76_RS01245at 255.885 kb on + strand, within BWI76_RS01245at 255.885 kb on + strand, within BWI76_RS01245at 255.963 kb on + strand, within BWI76_RS01245at 256.006 kb on + strand, within BWI76_RS01245at 256.258 kb on + strandat 256.357 kb on - strandat 256.376 kb on - strandat 256.408 kb on + strandat 256.535 kb on + strandat 256.543 kb on - strandat 256.633 kb on - strand, within BWI76_RS01250at 256.643 kb on + strand, within BWI76_RS01250at 256.643 kb on + strand, within BWI76_RS01250at 256.651 kb on - strand, within BWI76_RS01250at 256.656 kb on - strand, within BWI76_RS01250at 256.718 kb on + strand, within BWI76_RS01250at 256.807 kb on - strand, within BWI76_RS01250at 256.875 kb on - strand, within BWI76_RS01250at 257.129 kb on + strandat 257.137 kb on - strandat 257.179 kb on + strandat 257.379 kb on - strandat 257.379 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Maltose (C)
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253,615 - +1.2
253,657 + +0.2
253,664 + -0.5
253,664 + +1.1
253,672 - +0.2
253,672 - +0.6
253,743 + BWI76_RS01240 0.10 -0.1
253,815 + BWI76_RS01240 0.18 -0.1
253,823 - BWI76_RS01240 0.19 -0.1
253,939 - BWI76_RS01240 0.33 -0.5
254,025 - BWI76_RS01240 0.42 -0.6
254,123 + BWI76_RS01240 0.54 -0.3
254,316 + BWI76_RS01240 0.76 -0.8
254,331 + BWI76_RS01240 0.77 -0.2
254,339 - BWI76_RS01240 0.78 -0.4
254,427 + BWI76_RS01240 0.88 +0.5
254,437 + BWI76_RS01240 0.90 -0.3
254,639 + +0.3
254,703 + -1.2
254,722 + -1.4
254,722 + -0.5
254,769 - +0.1
254,831 + BWI76_RS01245 0.13 +0.2
254,901 - BWI76_RS01245 0.17 -0.0
255,222 - BWI76_RS01245 0.34 +0.5
255,654 - BWI76_RS01245 0.58 +0.1
255,695 + BWI76_RS01245 0.60 +0.8
255,739 + BWI76_RS01245 0.63 +0.4
255,885 + BWI76_RS01245 0.71 +1.6
255,885 + BWI76_RS01245 0.71 +1.2
255,885 + BWI76_RS01245 0.71 +0.2
255,963 + BWI76_RS01245 0.75 +0.5
256,006 + BWI76_RS01245 0.77 +0.5
256,258 + +0.6
256,357 - +0.4
256,376 - +0.5
256,408 + -1.6
256,535 + +0.1
256,543 - -1.3
256,633 - BWI76_RS01250 0.24 -0.4
256,643 + BWI76_RS01250 0.26 -0.1
256,643 + BWI76_RS01250 0.26 +0.4
256,651 - BWI76_RS01250 0.27 -0.9
256,656 - BWI76_RS01250 0.28 +0.4
256,718 + BWI76_RS01250 0.38 +0.4
256,807 - BWI76_RS01250 0.52 +1.6
256,875 - BWI76_RS01250 0.63 +0.3
257,129 + +1.2
257,137 - +0.1
257,179 + +0.2
257,379 - +1.6
257,379 - +0.0

Or see this region's nucleotide sequence