Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_296

Experiment: Aluminum chloride 0.00005 M

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_294 and Pf6N2E2_295 are separated by 57 nucleotidesPf6N2E2_295 and Pf6N2E2_296 are separated by 81 nucleotidesPf6N2E2_296 and Pf6N2E2_297 are separated by 308 nucleotides Pf6N2E2_294 - Arginine-tRNA-protein transferase (EC 2.3.2.8), at 363,417 to 364,124 _294 Pf6N2E2_295 - Leucyl/phenylalanyl-tRNA--protein transferase (EC 2.3.2.6), at 364,182 to 364,862 _295 Pf6N2E2_296 - Thioredoxin reductase (EC 1.8.1.9), at 364,944 to 365,903 _296 Pf6N2E2_297 - Cell division protein FtsK, at 366,212 to 368,617 _297 Position (kb) 364 365 366Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 363.972 kb on - strand, within Pf6N2E2_294at 364.097 kb on - strandat 364.147 kb on + strandat 364.148 kb on - strandat 364.153 kb on + strandat 364.153 kb on + strandat 364.153 kb on + strandat 364.154 kb on - strandat 364.159 kb on + strandat 364.159 kb on + strandat 364.160 kb on - strandat 364.160 kb on - strandat 364.160 kb on - strandat 364.231 kb on + strandat 364.232 kb on - strandat 364.419 kb on + strand, within Pf6N2E2_295at 364.427 kb on - strand, within Pf6N2E2_295at 364.544 kb on - strand, within Pf6N2E2_295at 364.606 kb on + strand, within Pf6N2E2_295at 364.606 kb on + strand, within Pf6N2E2_295at 364.768 kb on - strand, within Pf6N2E2_295at 364.867 kb on - strandat 364.878 kb on + strandat 364.878 kb on + strandat 364.878 kb on + strandat 364.878 kb on + strandat 364.878 kb on + strandat 364.879 kb on - strandat 364.879 kb on - strandat 364.879 kb on - strandat 364.879 kb on - strandat 364.879 kb on - strandat 364.879 kb on - strandat 364.879 kb on - strandat 364.969 kb on + strandat 364.969 kb on + strandat 364.969 kb on + strandat 364.969 kb on + strandat 364.970 kb on - strandat 364.970 kb on - strandat 364.970 kb on - strandat 364.970 kb on - strandat 365.015 kb on + strandat 365.015 kb on + strandat 365.015 kb on + strandat 365.016 kb on - strandat 365.016 kb on - strandat 365.016 kb on - strandat 365.110 kb on - strand, within Pf6N2E2_296at 365.151 kb on + strand, within Pf6N2E2_296at 365.152 kb on - strand, within Pf6N2E2_296at 365.169 kb on - strand, within Pf6N2E2_296at 365.169 kb on - strand, within Pf6N2E2_296at 365.177 kb on + strand, within Pf6N2E2_296at 365.187 kb on + strand, within Pf6N2E2_296at 365.306 kb on + strand, within Pf6N2E2_296at 365.307 kb on - strand, within Pf6N2E2_296at 365.413 kb on + strand, within Pf6N2E2_296at 365.413 kb on + strand, within Pf6N2E2_296at 365.413 kb on + strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.593 kb on + strand, within Pf6N2E2_296at 365.593 kb on + strand, within Pf6N2E2_296at 365.593 kb on + strand, within Pf6N2E2_296at 365.593 kb on + strand, within Pf6N2E2_296at 365.593 kb on + strand, within Pf6N2E2_296at 365.593 kb on + strand, within Pf6N2E2_296at 365.594 kb on - strand, within Pf6N2E2_296at 365.652 kb on - strand, within Pf6N2E2_296at 365.652 kb on - strand, within Pf6N2E2_296at 365.712 kb on + strand, within Pf6N2E2_296at 365.833 kb on + strandat 365.833 kb on + strandat 365.962 kb on + strandat 365.962 kb on + strandat 365.962 kb on + strandat 365.962 kb on + strandat 365.962 kb on + strandat 366.109 kb on + strandat 366.137 kb on + strandat 366.162 kb on + strandat 366.164 kb on + strandat 366.164 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Aluminum chloride 0.00005 M
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363,972 - Pf6N2E2_294 0.78 +1.4
364,097 - +0.3
364,147 + +0.3
364,148 - +0.5
364,153 + -0.3
364,153 + -0.6
364,153 + -0.8
364,154 - +0.7
364,159 + -0.4
364,159 + +0.4
364,160 - +1.0
364,160 - -0.8
364,160 - +1.2
364,231 + +1.1
364,232 - +1.2
364,419 + Pf6N2E2_295 0.35 -0.5
364,427 - Pf6N2E2_295 0.36 +0.1
364,544 - Pf6N2E2_295 0.53 -0.5
364,606 + Pf6N2E2_295 0.62 -1.0
364,606 + Pf6N2E2_295 0.62 -1.4
364,768 - Pf6N2E2_295 0.86 +0.6
364,867 - -0.3
364,878 + +2.2
364,878 + -1.0
364,878 + +0.8
364,878 + +0.3
364,878 + -0.2
364,879 - +0.9
364,879 - +1.4
364,879 - -0.1
364,879 - -2.8
364,879 - -0.4
364,879 - -0.5
364,879 - +0.3
364,969 + -1.1
364,969 + -0.4
364,969 + +1.2
364,969 + +0.2
364,970 - -0.7
364,970 - -0.2
364,970 - +0.8
364,970 - +0.3
365,015 + +1.1
365,015 + -0.4
365,015 + +1.6
365,016 - +0.6
365,016 - -0.9
365,016 - -0.3
365,110 - Pf6N2E2_296 0.17 +0.7
365,151 + Pf6N2E2_296 0.22 -0.6
365,152 - Pf6N2E2_296 0.22 +0.2
365,169 - Pf6N2E2_296 0.23 -0.2
365,169 - Pf6N2E2_296 0.23 -1.1
365,177 + Pf6N2E2_296 0.24 -0.8
365,187 + Pf6N2E2_296 0.25 +0.5
365,306 + Pf6N2E2_296 0.38 -0.4
365,307 - Pf6N2E2_296 0.38 -0.0
365,413 + Pf6N2E2_296 0.49 +0.3
365,413 + Pf6N2E2_296 0.49 -0.3
365,413 + Pf6N2E2_296 0.49 +0.8
365,414 - Pf6N2E2_296 0.49 -0.4
365,414 - Pf6N2E2_296 0.49 +0.1
365,414 - Pf6N2E2_296 0.49 -0.3
365,414 - Pf6N2E2_296 0.49 -1.9
365,414 - Pf6N2E2_296 0.49 -2.6
365,414 - Pf6N2E2_296 0.49 -1.4
365,414 - Pf6N2E2_296 0.49 +0.5
365,414 - Pf6N2E2_296 0.49 -0.7
365,414 - Pf6N2E2_296 0.49 -0.8
365,593 + Pf6N2E2_296 0.68 -0.3
365,593 + Pf6N2E2_296 0.68 -1.6
365,593 + Pf6N2E2_296 0.68 -0.2
365,593 + Pf6N2E2_296 0.68 -1.6
365,593 + Pf6N2E2_296 0.68 -2.3
365,593 + Pf6N2E2_296 0.68 -0.8
365,594 - Pf6N2E2_296 0.68 -1.2
365,652 - Pf6N2E2_296 0.74 +0.6
365,652 - Pf6N2E2_296 0.74 +0.6
365,712 + Pf6N2E2_296 0.80 -0.8
365,833 + +0.1
365,833 + +0.1
365,962 + -1.9
365,962 + -0.2
365,962 + +0.1
365,962 + +1.0
365,962 + -0.8
366,109 + -0.5
366,137 + +0.9
366,162 + -0.1
366,164 + -0.6
366,164 + -2.6

Or see this region's nucleotide sequence