Experiment: Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_20590 and IAI47_20595 are separated by 309 nucleotides IAI47_20595 and IAI47_20600 are separated by 104 nucleotides IAI47_20600 and IAI47_20605 are separated by 174 nucleotides
IAI47_20590: IAI47_20590 - lactaldehyde reductase, at 368,842 to 369,990
_20590
IAI47_20595: IAI47_20595 - GhoT/OrtT family toxin, at 370,300 to 370,473
_20595
IAI47_20600: IAI47_20600 - hypothetical protein, at 370,578 to 370,754
_20600
IAI47_20605: IAI47_20605 - MFS transporter, at 370,929 to 372,128
_20605
Position (kb) 370
371 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 369.580 kb on + strand, within IAI47_20590 at 369.580 kb on + strand, within IAI47_20590 at 369.580 kb on + strand, within IAI47_20590 at 369.580 kb on + strand, within IAI47_20590 at 369.580 kb on + strand, within IAI47_20590 at 369.580 kb on + strand, within IAI47_20590 at 369.580 kb on + strand, within IAI47_20590 at 369.580 kb on + strand, within IAI47_20590 at 369.580 kb on + strand, within IAI47_20590 at 369.580 kb on + strand, within IAI47_20590 at 369.580 kb on + strand, within IAI47_20590 at 369.581 kb on - strand, within IAI47_20590 at 369.581 kb on - strand, within IAI47_20590 at 369.581 kb on - strand, within IAI47_20590 at 369.581 kb on - strand, within IAI47_20590 at 369.581 kb on - strand, within IAI47_20590 at 369.581 kb on - strand, within IAI47_20590 at 369.591 kb on + strand, within IAI47_20590 at 369.591 kb on + strand, within IAI47_20590 at 369.655 kb on + strand, within IAI47_20590 at 369.656 kb on - strand, within IAI47_20590 at 369.657 kb on + strand, within IAI47_20590 at 369.674 kb on + strand, within IAI47_20590 at 369.674 kb on + strand, within IAI47_20590 at 369.674 kb on + strand, within IAI47_20590 at 369.674 kb on + strand, within IAI47_20590 at 369.674 kb on + strand, within IAI47_20590 at 369.675 kb on - strand, within IAI47_20590 at 369.675 kb on - strand, within IAI47_20590 at 369.764 kb on + strand, within IAI47_20590 at 369.875 kb on + strand, within IAI47_20590 at 369.876 kb on - strand at 369.974 kb on + strand at 369.974 kb on + strand at 369.974 kb on + strand at 369.974 kb on + strand at 369.974 kb on + strand at 369.975 kb on - strand at 369.975 kb on - strand at 369.975 kb on - strand at 369.976 kb on + strand at 369.976 kb on + strand at 369.976 kb on + strand at 369.977 kb on - strand at 369.977 kb on - strand at 369.977 kb on - strand at 369.977 kb on - strand at 369.988 kb on + strand at 369.988 kb on + strand at 369.989 kb on - strand at 370.010 kb on + strand at 370.010 kb on + strand at 370.010 kb on + strand at 370.010 kb on + strand at 370.010 kb on + strand at 370.010 kb on + strand at 370.010 kb on + strand at 370.011 kb on - strand at 370.011 kb on - strand at 370.013 kb on + strand at 370.013 kb on + strand at 370.013 kb on + strand at 370.013 kb on + strand at 370.013 kb on + strand at 370.013 kb on + strand at 370.013 kb on + strand at 370.014 kb on - strand at 370.014 kb on - strand at 370.015 kb on + strand at 370.015 kb on + strand at 370.015 kb on + strand at 370.015 kb on + strand at 370.016 kb on - strand at 370.044 kb on - strand at 370.060 kb on + strand at 370.061 kb on - strand at 370.071 kb on + strand at 370.071 kb on + strand at 370.071 kb on + strand at 370.071 kb on + strand at 370.072 kb on - strand at 370.087 kb on + strand at 370.088 kb on - strand at 370.088 kb on - strand at 370.166 kb on + strand at 370.166 kb on + strand at 370.166 kb on + strand at 370.167 kb on - strand at 370.197 kb on - strand at 370.212 kb on + strand at 370.213 kb on - strand at 370.279 kb on - strand at 370.296 kb on + strand at 370.296 kb on + strand at 370.297 kb on - strand at 370.309 kb on + strand at 370.310 kb on - strand at 370.330 kb on + strand, within IAI47_20595 at 370.330 kb on + strand, within IAI47_20595 at 370.330 kb on + strand, within IAI47_20595 at 370.383 kb on + strand, within IAI47_20595 at 370.383 kb on + strand, within IAI47_20595 at 370.384 kb on - strand, within IAI47_20595 at 370.384 kb on - strand, within IAI47_20595 at 370.384 kb on - strand, within IAI47_20595 at 370.384 kb on - strand, within IAI47_20595 at 370.384 kb on - strand, within IAI47_20595 at 370.384 kb on - strand, within IAI47_20595 at 370.415 kb on + strand, within IAI47_20595 at 370.471 kb on + strand at 370.471 kb on + strand at 370.472 kb on - strand at 370.489 kb on + strand at 370.501 kb on + strand at 370.501 kb on + strand at 370.501 kb on + strand at 370.502 kb on - strand at 370.502 kb on - strand at 370.502 kb on - strand at 370.502 kb on - strand at 370.506 kb on + strand at 370.506 kb on + strand at 370.506 kb on + strand at 370.506 kb on + strand at 370.507 kb on - strand at 370.507 kb on - strand at 370.507 kb on - strand at 370.507 kb on - strand at 370.507 kb on - strand at 370.544 kb on + strand at 370.545 kb on - strand at 370.563 kb on + strand at 370.564 kb on - strand at 370.566 kb on + strand at 370.567 kb on - strand at 370.567 kb on - strand at 370.617 kb on - strand, within IAI47_20600 at 370.617 kb on - strand, within IAI47_20600 at 370.617 kb on - strand, within IAI47_20600 at 370.617 kb on - strand, within IAI47_20600 at 370.760 kb on - strand at 370.804 kb on - strand at 370.804 kb on - strand at 370.809 kb on + strand at 370.809 kb on + strand at 370.809 kb on + strand at 370.809 kb on + strand at 370.809 kb on + strand at 370.809 kb on + strand at 370.809 kb on + strand at 370.809 kb on + strand at 370.810 kb on - strand at 370.810 kb on - strand at 370.810 kb on - strand at 370.810 kb on - strand at 370.810 kb on - strand at 370.819 kb on + strand at 370.819 kb on + strand at 370.819 kb on + strand at 370.820 kb on - strand at 370.840 kb on + strand at 370.840 kb on + strand at 370.841 kb on - strand at 370.841 kb on - strand at 370.841 kb on - strand at 370.841 kb on - strand at 370.872 kb on + strand at 370.872 kb on + strand at 370.872 kb on + strand at 370.872 kb on + strand at 370.872 kb on + strand at 370.873 kb on - strand at 370.873 kb on - strand at 370.939 kb on + strand at 370.939 kb on + strand at 370.939 kb on + strand at 370.939 kb on + strand at 370.940 kb on - strand at 370.940 kb on - strand at 370.940 kb on - strand at 370.953 kb on - strand at 370.961 kb on + strand at 370.979 kb on + strand at 371.006 kb on + strand at 371.006 kb on + strand at 371.007 kb on - strand at 371.007 kb on - strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.030 kb on + strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.031 kb on - strand at 371.033 kb on + strand at 371.061 kb on - strand, within IAI47_20605 at 371.105 kb on + strand, within IAI47_20605 at 371.120 kb on + strand, within IAI47_20605 at 371.120 kb on + strand, within IAI47_20605 at 371.120 kb on + strand, within IAI47_20605 at 371.121 kb on - strand, within IAI47_20605 at 371.121 kb on - strand, within IAI47_20605 at 371.121 kb on - strand, within IAI47_20605 at 371.121 kb on - strand, within IAI47_20605 at 371.276 kb on + strand, within IAI47_20605 at 371.277 kb on - strand, within IAI47_20605 at 371.277 kb on - strand, within IAI47_20605 at 371.348 kb on + strand, within IAI47_20605 at 371.352 kb on + strand, within IAI47_20605 at 371.352 kb on + strand, within IAI47_20605 at 371.352 kb on + strand, within IAI47_20605 at 371.352 kb on + strand, within IAI47_20605 at 371.352 kb on + strand, within IAI47_20605 at 371.352 kb on + strand, within IAI47_20605 at 371.352 kb on + strand, within IAI47_20605 at 371.352 kb on + strand, within IAI47_20605 at 371.352 kb on + strand, within IAI47_20605 at 371.352 kb on + strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.353 kb on - strand, within IAI47_20605 at 371.354 kb on + strand, within IAI47_20605 at 371.355 kb on - strand, within IAI47_20605 at 371.355 kb on - strand, within IAI47_20605 at 371.364 kb on + strand, within IAI47_20605 at 371.364 kb on + strand, within IAI47_20605 at 371.364 kb on + strand, within IAI47_20605 at 371.364 kb on + strand, within IAI47_20605 at 371.364 kb on + strand, within IAI47_20605 at 371.364 kb on + strand, within IAI47_20605 at 371.628 kb on + strand, within IAI47_20605 at 371.628 kb on + strand, within IAI47_20605 at 371.628 kb on + strand at 371.628 kb on + strand, within IAI47_20605 at 371.628 kb on + strand, within IAI47_20605 at 371.629 kb on - strand, within IAI47_20605 at 371.630 kb on + strand, within IAI47_20605 at 371.630 kb on + strand, within IAI47_20605 at 371.669 kb on + strand, within IAI47_20605 at 371.669 kb on + strand, within IAI47_20605 at 371.669 kb on + strand, within IAI47_20605 at 371.669 kb on + strand, within IAI47_20605 at 371.669 kb on + strand at 371.670 kb on - strand, within IAI47_20605 at 371.670 kb on - strand, within IAI47_20605 at 371.670 kb on - strand, within IAI47_20605 at 371.670 kb on - strand, within IAI47_20605 at 371.701 kb on + strand, within IAI47_20605 at 371.701 kb on + strand, within IAI47_20605 at 371.710 kb on - strand, within IAI47_20605 at 371.744 kb on + strand, within IAI47_20605 at 371.744 kb on + strand, within IAI47_20605 at 371.744 kb on + strand, within IAI47_20605 at 371.745 kb on - strand, within IAI47_20605 at 371.745 kb on - strand, within IAI47_20605 at 371.745 kb on - strand, within IAI47_20605 at 371.745 kb on - strand, within IAI47_20605 at 371.745 kb on - strand, within IAI47_20605 at 371.745 kb on - strand, within IAI47_20605 at 371.745 kb on - strand, within IAI47_20605
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 369,580 + IAI47_20590 0.64 +1.8 369,580 + IAI47_20590 0.64 -1.6 369,580 + IAI47_20590 0.64 +0.5 369,580 + IAI47_20590 0.64 -0.7 369,580 + IAI47_20590 0.64 -0.8 369,580 + IAI47_20590 0.64 -0.4 369,580 + IAI47_20590 0.64 -1.3 369,580 + IAI47_20590 0.64 -0.7 369,580 + IAI47_20590 0.64 -1.6 369,580 + IAI47_20590 0.64 -0.3 369,580 + IAI47_20590 0.64 -0.2 369,581 - IAI47_20590 0.64 +0.7 369,581 - IAI47_20590 0.64 +3.6 369,581 - IAI47_20590 0.64 +2.9 369,581 - IAI47_20590 0.64 -1.8 369,581 - IAI47_20590 0.64 -0.5 369,581 - IAI47_20590 0.64 +0.2 369,591 + IAI47_20590 0.65 -1.1 369,591 + IAI47_20590 0.65 -1.3 369,655 + IAI47_20590 0.71 -0.8 369,656 - IAI47_20590 0.71 +0.1 369,657 + IAI47_20590 0.71 +0.6 369,674 + IAI47_20590 0.72 +0.4 369,674 + IAI47_20590 0.72 -2.5 369,674 + IAI47_20590 0.72 +1.7 369,674 + IAI47_20590 0.72 -1.1 369,674 + IAI47_20590 0.72 +0.2 369,675 - IAI47_20590 0.72 +0.2 369,675 - IAI47_20590 0.72 -0.3 369,764 + IAI47_20590 0.80 -0.6 369,875 + IAI47_20590 0.90 +0.8 369,876 - -0.0 369,974 + -1.3 369,974 + +0.6 369,974 + -0.3 369,974 + -0.1 369,974 + +1.2 369,975 - +0.4 369,975 - -0.5 369,975 - -1.1 369,976 + +1.7 369,976 + -0.8 369,976 + -2.5 369,977 - -0.1 369,977 - +0.9 369,977 - -1.6 369,977 - -1.6 369,988 + -2.1 369,988 + +2.7 369,989 - -0.8 370,010 + -0.5 370,010 + +0.5 370,010 + +0.4 370,010 + -0.1 370,010 + +0.6 370,010 + +1.1 370,010 + -0.4 370,011 - +0.3 370,011 - +2.2 370,013 + +0.2 370,013 + +0.5 370,013 + -2.1 370,013 + +1.9 370,013 + -2.9 370,013 + -0.1 370,013 + +0.8 370,014 - +1.7 370,014 - +0.7 370,015 + -2.5 370,015 + +0.8 370,015 + -0.3 370,015 + +0.8 370,016 - +1.8 370,044 - -2.2 370,060 + +0.1 370,061 - -1.8 370,071 + -0.8 370,071 + +0.9 370,071 + +1.2 370,071 + -0.6 370,072 - +4.1 370,087 + -0.1 370,088 - +0.2 370,088 - -2.2 370,166 + -1.3 370,166 + -1.0 370,166 + -1.3 370,167 - +1.4 370,197 - -2.2 370,212 + +0.1 370,213 - -2.7 370,279 - -3.0 370,296 + +0.7 370,296 + +0.6 370,297 - +4.1 370,309 + +2.4 370,310 - -1.7 370,330 + IAI47_20595 0.17 +0.7 370,330 + IAI47_20595 0.17 -2.7 370,330 + IAI47_20595 0.17 +2.6 370,383 + IAI47_20595 0.48 -0.4 370,383 + IAI47_20595 0.48 -1.7 370,384 - IAI47_20595 0.48 -3.7 370,384 - IAI47_20595 0.48 -0.6 370,384 - IAI47_20595 0.48 +0.8 370,384 - IAI47_20595 0.48 -1.3 370,384 - IAI47_20595 0.48 +1.0 370,384 - IAI47_20595 0.48 +0.7 370,415 + IAI47_20595 0.66 +0.4 370,471 + -1.8 370,471 + -0.8 370,472 - -0.5 370,489 + +0.4 370,501 + -0.7 370,501 + -1.1 370,501 + +0.2 370,502 - +0.1 370,502 - -0.2 370,502 - -0.8 370,502 - -1.9 370,506 + +1.8 370,506 + +3.6 370,506 + -0.6 370,506 + -2.6 370,507 - -1.3 370,507 - +0.9 370,507 - -0.8 370,507 - -0.8 370,507 - -1.6 370,544 + +0.4 370,545 - -0.3 370,563 + +0.1 370,564 - -0.2 370,566 + -2.3 370,567 - -0.8 370,567 - +0.0 370,617 - IAI47_20600 0.22 +0.2 370,617 - IAI47_20600 0.22 -2.5 370,617 - IAI47_20600 0.22 -4.0 370,617 - IAI47_20600 0.22 -3.0 370,760 - -0.0 370,804 - +3.1 370,804 - +1.4 370,809 + -1.9 370,809 + -2.3 370,809 + -0.8 370,809 + +1.2 370,809 + -0.3 370,809 + -0.8 370,809 + +1.2 370,809 + -0.8 370,810 - -0.8 370,810 - -1.8 370,810 - +0.5 370,810 - +0.2 370,810 - +1.8 370,819 + -0.8 370,819 + -2.2 370,819 + +0.7 370,820 - -1.1 370,840 + -0.2 370,840 + -0.1 370,841 - -0.6 370,841 - -0.6 370,841 - +0.4 370,841 - +1.7 370,872 + -1.1 370,872 + -2.4 370,872 + +3.6 370,872 + -2.1 370,872 + +0.5 370,873 - -1.3 370,873 - +0.8 370,939 + -0.3 370,939 + -0.9 370,939 + +1.9 370,939 + +0.3 370,940 - +0.1 370,940 - -0.3 370,940 - +0.9 370,953 - -2.1 370,961 + -1.1 370,979 + -0.7 371,006 + +0.2 371,006 + -2.8 371,007 - +1.0 371,007 - -1.8 371,030 + +1.8 371,030 + -3.3 371,030 + +1.2 371,030 + +2.1 371,030 + -2.9 371,030 + +1.2 371,030 + -0.0 371,030 + -0.6 371,030 + +2.0 371,030 + -0.1 371,030 + +1.1 371,030 + -1.8 371,030 + +1.0 371,030 + +2.1 371,030 + -2.3 371,030 + -0.3 371,031 - -1.6 371,031 - -2.1 371,031 - -2.2 371,031 - -3.8 371,031 - +1.2 371,031 - +0.5 371,031 - -0.6 371,031 - +0.2 371,031 - +1.6 371,031 - +2.3 371,031 - +1.8 371,031 - -0.6 371,031 - +2.0 371,031 - +1.6 371,031 - +0.8 371,033 + -1.8 371,061 - IAI47_20605 0.11 +1.7 371,105 + IAI47_20605 0.15 +1.2 371,120 + IAI47_20605 0.16 +2.8 371,120 + IAI47_20605 0.16 +0.0 371,120 + IAI47_20605 0.16 -2.9 371,121 - IAI47_20605 0.16 +0.5 371,121 - IAI47_20605 0.16 -2.5 371,121 - IAI47_20605 0.16 +1.5 371,121 - IAI47_20605 0.16 -0.6 371,276 + IAI47_20605 0.29 +1.0 371,277 - IAI47_20605 0.29 -0.5 371,277 - IAI47_20605 0.29 +0.9 371,348 + IAI47_20605 0.35 -1.3 371,352 + IAI47_20605 0.35 +1.6 371,352 + IAI47_20605 0.35 +4.5 371,352 + IAI47_20605 0.35 -2.5 371,352 + IAI47_20605 0.35 -2.0 371,352 + IAI47_20605 0.35 -1.6 371,352 + IAI47_20605 0.35 +0.8 371,352 + IAI47_20605 0.35 -1.9 371,352 + IAI47_20605 0.35 +0.2 371,352 + IAI47_20605 0.35 +2.8 371,352 + IAI47_20605 0.35 +0.1 371,353 - IAI47_20605 0.35 -0.1 371,353 - IAI47_20605 0.35 -1.8 371,353 - IAI47_20605 0.35 +1.2 371,353 - IAI47_20605 0.35 +2.8 371,353 - IAI47_20605 0.35 -0.1 371,353 - IAI47_20605 0.35 +0.8 371,353 - IAI47_20605 0.35 +1.2 371,353 - IAI47_20605 0.35 -0.3 371,353 - IAI47_20605 0.35 +2.2 371,353 - IAI47_20605 0.35 -0.6 371,353 - IAI47_20605 0.35 -0.8 371,353 - IAI47_20605 0.35 -3.2 371,353 - IAI47_20605 0.35 -0.8 371,353 - IAI47_20605 0.35 +0.4 371,354 + IAI47_20605 0.35 -1.1 371,355 - IAI47_20605 0.35 -1.1 371,355 - IAI47_20605 0.35 +1.2 371,364 + IAI47_20605 0.36 +2.8 371,364 + IAI47_20605 0.36 +1.6 371,364 + IAI47_20605 0.36 +0.0 371,364 + IAI47_20605 0.36 -1.9 371,364 + IAI47_20605 0.36 -0.2 371,364 + IAI47_20605 0.36 +1.9 371,628 + IAI47_20605 0.58 +0.2 371,628 + IAI47_20605 0.58 +1.1 371,628 + +0.2 371,628 + IAI47_20605 0.58 +0.2 371,628 + IAI47_20605 0.58 +0.5 371,629 - IAI47_20605 0.58 -2.1 371,630 + IAI47_20605 0.58 -0.8 371,630 + IAI47_20605 0.58 +1.2 371,669 + IAI47_20605 0.62 -0.2 371,669 + IAI47_20605 0.62 +1.2 371,669 + IAI47_20605 0.62 -1.4 371,669 + IAI47_20605 0.62 +2.6 371,669 + +1.2 371,670 - IAI47_20605 0.62 -0.3 371,670 - IAI47_20605 0.62 -1.6 371,670 - IAI47_20605 0.62 -1.6 371,670 - IAI47_20605 0.62 -1.3 371,701 + IAI47_20605 0.64 +2.6 371,701 + IAI47_20605 0.64 -0.8 371,710 - IAI47_20605 0.65 +0.4 371,744 + IAI47_20605 0.68 -0.1 371,744 + IAI47_20605 0.68 +1.2 371,744 + IAI47_20605 0.68 +0.9 371,745 - IAI47_20605 0.68 +3.5 371,745 - IAI47_20605 0.68 +1.2 371,745 - IAI47_20605 0.68 -2.1 371,745 - IAI47_20605 0.68 +1.2 371,745 - IAI47_20605 0.68 +2.5 371,745 - IAI47_20605 0.68 +0.1 371,745 - IAI47_20605 0.68 -0.6
Or see this region's nucleotide sequence