Experiment: Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_06360 and IAI47_06365 are separated by 15 nucleotides IAI47_06365 and IAI47_06370 are separated by 113 nucleotides IAI47_06370 and IAI47_06375 are separated by 17 nucleotides IAI47_06375 and IAI47_06380 are separated by 2 nucleotides
IAI47_06360: IAI47_06360 - NADH-quinone oxidoreductase subunit A, at 1,293,849 to 1,294,292
_06360
IAI47_06365: IAI47_06365 - NADH-quinone oxidoreductase subunit B, at 1,294,308 to 1,294,985
_06365
IAI47_06370: IAI47_06370 - NADH-quinone oxidoreductase subunit C/D, at 1,295,099 to 1,296,898
_06370
IAI47_06375: IAI47_06375 - NADH-quinone oxidoreductase subunit NuoE, at 1,296,916 to 1,297,416
_06375
IAI47_06380: IAI47_06380 - NADH-quinone oxidoreductase subunit NuoF, at 1,297,419 to 1,298,759
_06380
Position (kb)
1295
1296
1297 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 1294.104 kb on + strand, within IAI47_06360 at 1294.194 kb on + strand, within IAI47_06360 at 1294.243 kb on + strand, within IAI47_06360 at 1294.243 kb on + strand, within IAI47_06360 at 1294.256 kb on + strand at 1294.259 kb on + strand at 1294.259 kb on + strand at 1294.259 kb on + strand at 1294.259 kb on + strand at 1294.259 kb on + strand at 1294.259 kb on + strand at 1294.283 kb on + strand at 1294.284 kb on - strand at 1294.290 kb on + strand at 1294.290 kb on + strand at 1294.304 kb on + strand at 1294.305 kb on - strand at 1294.314 kb on + strand at 1294.315 kb on - strand at 1294.316 kb on + strand at 1294.316 kb on + strand at 1294.422 kb on + strand, within IAI47_06365 at 1294.422 kb on + strand, within IAI47_06365 at 1294.422 kb on + strand, within IAI47_06365 at 1294.423 kb on - strand, within IAI47_06365 at 1294.423 kb on - strand, within IAI47_06365 at 1294.425 kb on - strand, within IAI47_06365 at 1294.516 kb on - strand, within IAI47_06365 at 1294.661 kb on - strand, within IAI47_06365 at 1294.662 kb on + strand, within IAI47_06365 at 1294.663 kb on - strand, within IAI47_06365 at 1294.663 kb on - strand, within IAI47_06365 at 1294.728 kb on + strand, within IAI47_06365 at 1294.728 kb on + strand, within IAI47_06365 at 1294.729 kb on - strand, within IAI47_06365 at 1294.729 kb on - strand, within IAI47_06365 at 1294.738 kb on - strand, within IAI47_06365 at 1294.774 kb on + strand, within IAI47_06365 at 1294.774 kb on + strand, within IAI47_06365 at 1294.774 kb on + strand, within IAI47_06365 at 1294.775 kb on - strand, within IAI47_06365 at 1294.782 kb on + strand, within IAI47_06365 at 1294.782 kb on + strand, within IAI47_06365 at 1294.782 kb on + strand at 1294.782 kb on + strand, within IAI47_06365 at 1294.782 kb on + strand, within IAI47_06365 at 1294.783 kb on - strand, within IAI47_06365 at 1294.816 kb on - strand at 1294.816 kb on - strand at 1295.031 kb on + strand at 1295.031 kb on + strand at 1295.031 kb on + strand at 1295.093 kb on + strand at 1295.093 kb on + strand at 1295.098 kb on + strand at 1295.099 kb on - strand at 1295.099 kb on - strand at 1295.099 kb on - strand at 1295.099 kb on - strand at 1295.291 kb on - strand, within IAI47_06370 at 1295.307 kb on - strand, within IAI47_06370 at 1295.318 kb on + strand, within IAI47_06370 at 1295.346 kb on + strand, within IAI47_06370 at 1295.346 kb on + strand, within IAI47_06370 at 1295.347 kb on - strand, within IAI47_06370 at 1295.351 kb on - strand, within IAI47_06370 at 1295.504 kb on + strand at 1295.504 kb on + strand, within IAI47_06370 at 1295.505 kb on - strand, within IAI47_06370 at 1295.537 kb on - strand, within IAI47_06370 at 1295.832 kb on + strand, within IAI47_06370 at 1295.832 kb on + strand, within IAI47_06370 at 1295.832 kb on + strand, within IAI47_06370 at 1295.897 kb on + strand, within IAI47_06370 at 1295.897 kb on + strand, within IAI47_06370 at 1295.897 kb on + strand, within IAI47_06370 at 1295.898 kb on - strand, within IAI47_06370 at 1295.906 kb on + strand, within IAI47_06370 at 1295.907 kb on - strand, within IAI47_06370 at 1295.907 kb on - strand, within IAI47_06370 at 1295.954 kb on + strand, within IAI47_06370 at 1295.982 kb on + strand, within IAI47_06370 at 1296.060 kb on - strand, within IAI47_06370 at 1296.061 kb on + strand, within IAI47_06370 at 1296.276 kb on - strand, within IAI47_06370 at 1296.276 kb on - strand, within IAI47_06370 at 1296.335 kb on + strand, within IAI47_06370 at 1296.341 kb on - strand, within IAI47_06370 at 1296.425 kb on + strand, within IAI47_06370 at 1296.486 kb on - strand, within IAI47_06370 at 1296.486 kb on - strand, within IAI47_06370 at 1296.532 kb on + strand, within IAI47_06370 at 1296.556 kb on + strand, within IAI47_06370 at 1296.557 kb on - strand, within IAI47_06370 at 1296.557 kb on - strand, within IAI47_06370 at 1296.617 kb on - strand at 1296.731 kb on + strand at 1296.764 kb on + strand at 1296.852 kb on + strand at 1296.852 kb on + strand at 1296.853 kb on - strand at 1296.854 kb on + strand at 1296.855 kb on - strand at 1296.855 kb on - strand at 1296.863 kb on - strand at 1296.866 kb on - strand at 1296.878 kb on - strand at 1296.896 kb on + strand at 1296.900 kb on + strand at 1296.900 kb on + strand at 1296.900 kb on + strand at 1296.901 kb on - strand at 1296.901 kb on - strand at 1296.901 kb on - strand at 1296.924 kb on + strand at 1296.939 kb on + strand at 1297.098 kb on - strand at 1297.098 kb on - strand, within IAI47_06375 at 1297.186 kb on + strand, within IAI47_06375 at 1297.219 kb on + strand, within IAI47_06375 at 1297.437 kb on - strand at 1297.495 kb on + strand at 1297.521 kb on + strand at 1297.521 kb on + strand at 1297.713 kb on + strand, within IAI47_06380 at 1297.714 kb on - strand, within IAI47_06380 at 1297.813 kb on + strand, within IAI47_06380 at 1297.824 kb on + strand, within IAI47_06380 at 1297.825 kb on - strand, within IAI47_06380 at 1297.890 kb on - strand, within IAI47_06380
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 1,294,104 + IAI47_06360 0.57 +3.5 1,294,194 + IAI47_06360 0.78 +1.5 1,294,243 + IAI47_06360 0.89 -1.0 1,294,243 + IAI47_06360 0.89 +0.8 1,294,256 + +1.5 1,294,259 + +0.1 1,294,259 + +2.1 1,294,259 + +0.2 1,294,259 + +0.9 1,294,259 + +2.0 1,294,259 + -1.7 1,294,283 + +1.5 1,294,284 - +0.5 1,294,290 + +3.2 1,294,290 + +2.9 1,294,304 + +3.3 1,294,305 - +2.8 1,294,314 + +3.1 1,294,315 - +2.7 1,294,316 + +2.8 1,294,316 + -1.4 1,294,422 + IAI47_06365 0.17 -0.1 1,294,422 + IAI47_06365 0.17 +3.5 1,294,422 + IAI47_06365 0.17 +3.5 1,294,423 - IAI47_06365 0.17 +3.0 1,294,423 - IAI47_06365 0.17 +5.2 1,294,425 - IAI47_06365 0.17 +0.1 1,294,516 - IAI47_06365 0.31 +3.1 1,294,661 - IAI47_06365 0.52 +3.6 1,294,662 + IAI47_06365 0.52 +3.3 1,294,663 - IAI47_06365 0.52 +2.3 1,294,663 - IAI47_06365 0.52 +3.6 1,294,728 + IAI47_06365 0.62 -1.1 1,294,728 + IAI47_06365 0.62 +3.1 1,294,729 - IAI47_06365 0.62 +1.5 1,294,729 - IAI47_06365 0.62 +3.7 1,294,738 - IAI47_06365 0.63 +1.1 1,294,774 + IAI47_06365 0.69 -0.2 1,294,774 + IAI47_06365 0.69 +0.3 1,294,774 + IAI47_06365 0.69 +1.5 1,294,775 - IAI47_06365 0.69 +4.4 1,294,782 + IAI47_06365 0.70 +2.3 1,294,782 + IAI47_06365 0.70 +1.8 1,294,782 + -1.1 1,294,782 + IAI47_06365 0.70 +2.0 1,294,782 + IAI47_06365 0.70 -0.1 1,294,783 - IAI47_06365 0.70 +3.8 1,294,816 - +1.1 1,294,816 - +4.1 1,295,031 + +3.1 1,295,031 + -0.4 1,295,031 + +1.4 1,295,093 + +4.1 1,295,093 + +3.1 1,295,098 + -0.7 1,295,099 - +3.0 1,295,099 - +3.8 1,295,099 - -0.1 1,295,099 - +4.1 1,295,291 - IAI47_06370 0.11 +5.4 1,295,307 - IAI47_06370 0.12 +2.9 1,295,318 + IAI47_06370 0.12 +1.5 1,295,346 + IAI47_06370 0.14 +0.5 1,295,346 + IAI47_06370 0.14 +2.8 1,295,347 - IAI47_06370 0.14 +4.0 1,295,351 - IAI47_06370 0.14 +5.6 1,295,504 + +3.5 1,295,504 + IAI47_06370 0.23 +0.7 1,295,505 - IAI47_06370 0.23 +3.8 1,295,537 - IAI47_06370 0.24 +4.6 1,295,832 + IAI47_06370 0.41 +3.0 1,295,832 + IAI47_06370 0.41 +2.5 1,295,832 + IAI47_06370 0.41 +0.5 1,295,897 + IAI47_06370 0.44 -0.1 1,295,897 + IAI47_06370 0.44 +2.9 1,295,897 + IAI47_06370 0.44 +3.8 1,295,898 - IAI47_06370 0.44 +2.5 1,295,906 + IAI47_06370 0.45 +4.0 1,295,907 - IAI47_06370 0.45 +2.0 1,295,907 - IAI47_06370 0.45 +2.7 1,295,954 + IAI47_06370 0.47 +3.0 1,295,982 + IAI47_06370 0.49 +2.3 1,296,060 - IAI47_06370 0.53 +3.6 1,296,061 + IAI47_06370 0.53 +4.0 1,296,276 - IAI47_06370 0.65 +5.1 1,296,276 - IAI47_06370 0.65 +4.3 1,296,335 + IAI47_06370 0.69 +1.5 1,296,341 - IAI47_06370 0.69 +2.1 1,296,425 + IAI47_06370 0.74 -3.7 1,296,486 - IAI47_06370 0.77 +3.1 1,296,486 - IAI47_06370 0.77 +6.0 1,296,532 + IAI47_06370 0.80 +2.7 1,296,556 + IAI47_06370 0.81 +4.7 1,296,557 - IAI47_06370 0.81 +3.1 1,296,557 - IAI47_06370 0.81 +4.0 1,296,617 - +4.5 1,296,731 + +1.5 1,296,764 + +1.3 1,296,852 + +2.7 1,296,852 + -0.5 1,296,853 - +1.9 1,296,854 + +0.5 1,296,855 - +0.5 1,296,855 - +3.1 1,296,863 - +2.5 1,296,866 - +2.5 1,296,878 - +2.5 1,296,896 + +2.8 1,296,900 + +2.7 1,296,900 + +3.6 1,296,900 + +2.1 1,296,901 - +3.5 1,296,901 - +3.1 1,296,901 - +5.0 1,296,924 + +0.5 1,296,939 + +1.5 1,297,098 - +1.5 1,297,098 - IAI47_06375 0.36 +2.5 1,297,186 + IAI47_06375 0.54 +2.7 1,297,219 + IAI47_06375 0.60 +3.3 1,297,437 - +3.7 1,297,495 + +3.1 1,297,521 + +4.2 1,297,521 + -0.1 1,297,713 + IAI47_06380 0.22 +2.0 1,297,714 - IAI47_06380 0.22 -1.8 1,297,813 + IAI47_06380 0.29 +4.7 1,297,824 + IAI47_06380 0.30 +3.5 1,297,825 - IAI47_06380 0.30 -0.1 1,297,890 - IAI47_06380 0.35 +5.3
Or see this region's nucleotide sequence