Experiment: Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt IAI47_00240 and IAI47_00245 are separated by 154 nucleotides IAI47_00245 and IAI47_00250 are separated by 160 nucleotides IAI47_00250 and IAI47_00255 are separated by 24 nucleotides
IAI47_00240: IAI47_00240 - glutamine--fructose-6-phosphate transaminase (isomerizing), at 52,125 to 53,954
_00240
IAI47_00245: IAI47_00245 - bifunctional UDP-N-acetylglucosamine diphosphorylase/glucosamine-1-phosphate N-acetyltransferase GlmU, at 54,109 to 55,479
_00245
IAI47_00250: IAI47_00250 - F0F1 ATP synthase subunit epsilon, at 55,640 to 56,059
_00250
IAI47_00255: IAI47_00255 - F0F1 ATP synthase subunit beta, at 56,084 to 57,481
_00255
Position (kb) 54
55
56 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 53.973 kb on - strand at 53.973 kb on - strand at 53.973 kb on - strand at 53.973 kb on - strand at 53.973 kb on - strand at 53.973 kb on - strand at 53.973 kb on - strand at 54.042 kb on - strand at 54.042 kb on - strand at 54.042 kb on - strand at 54.042 kb on - strand at 54.056 kb on - strand at 54.057 kb on + strand at 54.057 kb on + strand at 54.057 kb on + strand at 54.057 kb on + strand at 54.057 kb on + strand at 54.058 kb on - strand at 54.058 kb on - strand at 54.058 kb on - strand at 54.058 kb on - strand at 54.058 kb on - strand at 55.513 kb on - strand at 55.513 kb on - strand at 55.513 kb on - strand at 55.513 kb on - strand at 55.535 kb on - strand at 55.535 kb on - strand at 55.535 kb on - strand at 55.535 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Survival; Treatment=FW300 (pH7.4); TreatmentDuration=24hrs; Outgrowth=LBkan50 remove 53,973 - -0.2 53,973 - -0.3 53,973 - +0.1 53,973 - +0.4 53,973 - -1.0 53,973 - -1.0 53,973 - +1.6 54,042 - -0.3 54,042 - -0.2 54,042 - -0.8 54,042 - -0.5 54,056 - +0.4 54,057 + -1.7 54,057 + -2.8 54,057 + -1.0 54,057 + +2.8 54,057 + -2.2 54,058 - +1.5 54,058 - +2.3 54,058 - +1.1 54,058 - -1.2 54,058 - +1.8 55,513 - +0.8 55,513 - -2.2 55,513 - -0.7 55,513 - -1.8 55,535 - -0.1 55,535 - -1.3 55,535 - +1.5 55,535 - +1.0
Or see this region's nucleotide sequence