Experiment: Recovery broth with Chenodeoxycholic 1.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS18315 and BBR_RS18320 are separated by 174 nucleotides BBR_RS18320 and BBR_RS18325 are separated by 303 nucleotides BBR_RS18325 and BBR_RS18330 are separated by 10 nucleotides BBR_RS18330 and BBR_RS18335 are separated by 32 nucleotides
BBR_RS18315: BBR_RS18315 - hypothetical protein, at 1,897,587 to 1,898,126
_RS18315
BBR_RS18320: BBR_RS18320 - hypothetical protein, at 1,898,301 to 1,898,726
_RS18320
BBR_RS18325: BBR_RS18325 - DNA-binding protein, at 1,899,030 to 1,899,476
_RS18325
BBR_RS18330: BBR_RS18330 - PIN domain-containing protein, at 1,899,487 to 1,900,056
_RS18330
BBR_RS18335: BBR_RS18335 - hypothetical protein, at 1,900,089 to 1,901,357
_RS18335
Position (kb)
1899
1900 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1898.043 kb on - strand, within BBR_RS18315 at 1898.108 kb on + strand at 1898.116 kb on - strand at 1898.122 kb on + strand at 1898.129 kb on + strand at 1898.129 kb on + strand at 1898.130 kb on - strand at 1898.151 kb on - strand at 1898.157 kb on - strand at 1898.167 kb on + strand at 1898.167 kb on + strand at 1898.177 kb on + strand at 1898.203 kb on + strand at 1898.211 kb on - strand at 1898.211 kb on - strand at 1898.213 kb on - strand at 1898.220 kb on + strand at 1898.228 kb on - strand at 1898.228 kb on - strand at 1898.230 kb on - strand at 1898.259 kb on - strand at 1898.350 kb on + strand, within BBR_RS18320 at 1898.351 kb on + strand, within BBR_RS18320 at 1898.359 kb on + strand, within BBR_RS18320 at 1898.418 kb on - strand, within BBR_RS18320 at 1898.455 kb on + strand, within BBR_RS18320 at 1898.479 kb on + strand, within BBR_RS18320 at 1898.488 kb on - strand, within BBR_RS18320 at 1898.545 kb on + strand, within BBR_RS18320 at 1898.545 kb on + strand, within BBR_RS18320 at 1898.545 kb on + strand, within BBR_RS18320 at 1898.553 kb on - strand, within BBR_RS18320 at 1898.576 kb on - strand, within BBR_RS18320 at 1898.600 kb on - strand, within BBR_RS18320 at 1898.666 kb on - strand, within BBR_RS18320 at 1898.675 kb on + strand, within BBR_RS18320 at 1898.683 kb on - strand, within BBR_RS18320 at 1898.683 kb on - strand, within BBR_RS18320 at 1898.708 kb on + strand at 1898.708 kb on + strand at 1898.708 kb on + strand at 1898.708 kb on + strand at 1898.742 kb on + strand at 1898.742 kb on + strand at 1898.750 kb on - strand at 1898.780 kb on + strand at 1898.783 kb on - strand at 1898.855 kb on + strand at 1898.862 kb on + strand at 1898.862 kb on + strand at 1898.863 kb on - strand at 1898.863 kb on - strand at 1898.863 kb on - strand at 1898.912 kb on + strand at 1898.947 kb on + strand at 1898.967 kb on - strand at 1899.046 kb on + strand at 1899.048 kb on - strand at 1899.054 kb on - strand at 1899.065 kb on + strand at 1899.067 kb on + strand at 1899.067 kb on + strand at 1899.074 kb on + strand at 1899.108 kb on + strand, within BBR_RS18325 at 1899.111 kb on + strand, within BBR_RS18325 at 1899.128 kb on + strand, within BBR_RS18325 at 1899.128 kb on + strand, within BBR_RS18325 at 1899.166 kb on - strand, within BBR_RS18325 at 1899.497 kb on - strand at 1899.515 kb on - strand at 1899.549 kb on + strand, within BBR_RS18330 at 1899.557 kb on - strand, within BBR_RS18330 at 1899.558 kb on - strand, within BBR_RS18330 at 1899.565 kb on - strand, within BBR_RS18330 at 1899.588 kb on - strand, within BBR_RS18330 at 1899.594 kb on - strand, within BBR_RS18330 at 1899.619 kb on - strand, within BBR_RS18330 at 1899.627 kb on + strand, within BBR_RS18330 at 1899.627 kb on + strand, within BBR_RS18330 at 1899.635 kb on - strand, within BBR_RS18330 at 1899.677 kb on - strand, within BBR_RS18330 at 1899.686 kb on + strand, within BBR_RS18330 at 1899.686 kb on + strand, within BBR_RS18330 at 1899.686 kb on + strand, within BBR_RS18330 at 1899.686 kb on + strand, within BBR_RS18330 at 1899.694 kb on - strand, within BBR_RS18330 at 1899.695 kb on - strand, within BBR_RS18330 at 1899.713 kb on + strand, within BBR_RS18330 at 1899.713 kb on + strand, within BBR_RS18330 at 1899.721 kb on - strand, within BBR_RS18330 at 1899.721 kb on - strand, within BBR_RS18330 at 1899.731 kb on - strand, within BBR_RS18330 at 1899.731 kb on - strand, within BBR_RS18330 at 1899.742 kb on - strand, within BBR_RS18330 at 1899.817 kb on + strand, within BBR_RS18330 at 1899.817 kb on - strand, within BBR_RS18330 at 1899.825 kb on + strand, within BBR_RS18330 at 1899.825 kb on + strand, within BBR_RS18330 at 1899.882 kb on + strand, within BBR_RS18330 at 1899.890 kb on - strand, within BBR_RS18330 at 1899.890 kb on - strand, within BBR_RS18330 at 1899.890 kb on - strand, within BBR_RS18330 at 1899.890 kb on - strand, within BBR_RS18330 at 1899.895 kb on - strand, within BBR_RS18330 at 1899.921 kb on - strand, within BBR_RS18330 at 1899.987 kb on - strand, within BBR_RS18330 at 1899.987 kb on - strand, within BBR_RS18330 at 1899.989 kb on - strand, within BBR_RS18330 at 1899.990 kb on + strand, within BBR_RS18330 at 1899.990 kb on + strand, within BBR_RS18330 at 1899.990 kb on + strand, within BBR_RS18330 at 1899.998 kb on - strand, within BBR_RS18330 at 1899.998 kb on - strand, within BBR_RS18330 at 1899.998 kb on - strand, within BBR_RS18330 at 1899.998 kb on - strand, within BBR_RS18330 at 1899.998 kb on - strand, within BBR_RS18330 at 1899.998 kb on - strand, within BBR_RS18330 at 1899.998 kb on - strand, within BBR_RS18330 at 1899.998 kb on - strand, within BBR_RS18330 at 1900.036 kb on + strand at 1900.062 kb on - strand at 1900.062 kb on - strand at 1900.064 kb on - strand at 1900.113 kb on + strand at 1900.113 kb on + strand at 1900.113 kb on + strand at 1900.116 kb on - strand at 1900.116 kb on - strand at 1900.121 kb on - strand at 1900.121 kb on - strand at 1900.121 kb on - strand at 1900.160 kb on + strand at 1900.167 kb on - strand at 1900.203 kb on - strand at 1900.221 kb on - strand, within BBR_RS18335 at 1900.240 kb on - strand, within BBR_RS18335 at 1900.240 kb on - strand, within BBR_RS18335 at 1900.251 kb on + strand, within BBR_RS18335 at 1900.282 kb on + strand, within BBR_RS18335 at 1900.331 kb on + strand, within BBR_RS18335 at 1900.344 kb on - strand, within BBR_RS18335 at 1900.374 kb on - strand, within BBR_RS18335 at 1900.401 kb on - strand, within BBR_RS18335 at 1900.413 kb on - strand, within BBR_RS18335 at 1900.417 kb on + strand, within BBR_RS18335 at 1900.444 kb on + strand, within BBR_RS18335 at 1900.460 kb on - strand, within BBR_RS18335
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Chenodeoxycholic 1.3 mM remove 1,898,043 - BBR_RS18315 0.84 +0.4 1,898,108 + +0.2 1,898,116 - +1.4 1,898,122 + +0.7 1,898,129 + +0.1 1,898,129 + +0.3 1,898,130 - -0.6 1,898,151 - -0.1 1,898,157 - +2.1 1,898,167 + -1.1 1,898,167 + -0.6 1,898,177 + -1.1 1,898,203 + +1.7 1,898,211 - +0.4 1,898,211 - +0.5 1,898,213 - -0.3 1,898,220 + -0.2 1,898,228 - +1.6 1,898,228 - +0.2 1,898,230 - +0.1 1,898,259 - -0.3 1,898,350 + BBR_RS18320 0.12 -1.3 1,898,351 + BBR_RS18320 0.12 -0.8 1,898,359 + BBR_RS18320 0.14 +0.2 1,898,418 - BBR_RS18320 0.27 -0.9 1,898,455 + BBR_RS18320 0.36 +0.7 1,898,479 + BBR_RS18320 0.42 +0.6 1,898,488 - BBR_RS18320 0.44 -1.0 1,898,545 + BBR_RS18320 0.57 +0.4 1,898,545 + BBR_RS18320 0.57 +1.0 1,898,545 + BBR_RS18320 0.57 +0.1 1,898,553 - BBR_RS18320 0.59 -0.1 1,898,576 - BBR_RS18320 0.65 -1.1 1,898,600 - BBR_RS18320 0.70 +0.5 1,898,666 - BBR_RS18320 0.86 +0.3 1,898,675 + BBR_RS18320 0.88 +1.8 1,898,683 - BBR_RS18320 0.90 +0.6 1,898,683 - BBR_RS18320 0.90 -0.2 1,898,708 + +0.4 1,898,708 + +0.7 1,898,708 + +0.1 1,898,708 + +1.3 1,898,742 + -0.2 1,898,742 + -0.3 1,898,750 - -0.5 1,898,780 + -1.2 1,898,783 - -0.8 1,898,855 + +0.4 1,898,862 + +1.0 1,898,862 + +0.3 1,898,863 - -0.4 1,898,863 - -0.4 1,898,863 - -0.1 1,898,912 + +0.4 1,898,947 + +0.1 1,898,967 - +0.7 1,899,046 + -0.9 1,899,048 - -2.0 1,899,054 - -0.3 1,899,065 + -1.2 1,899,067 + +0.3 1,899,067 + -1.3 1,899,074 + -1.5 1,899,108 + BBR_RS18325 0.17 -0.5 1,899,111 + BBR_RS18325 0.18 -0.9 1,899,128 + BBR_RS18325 0.22 -0.5 1,899,128 + BBR_RS18325 0.22 -1.1 1,899,166 - BBR_RS18325 0.30 -0.1 1,899,497 - +1.2 1,899,515 - -0.1 1,899,549 + BBR_RS18330 0.11 +0.7 1,899,557 - BBR_RS18330 0.12 +0.7 1,899,558 - BBR_RS18330 0.12 -1.3 1,899,565 - BBR_RS18330 0.14 +1.3 1,899,588 - BBR_RS18330 0.18 +0.0 1,899,594 - BBR_RS18330 0.19 -0.4 1,899,619 - BBR_RS18330 0.23 +1.0 1,899,627 + BBR_RS18330 0.25 -0.2 1,899,627 + BBR_RS18330 0.25 -1.2 1,899,635 - BBR_RS18330 0.26 -0.4 1,899,677 - BBR_RS18330 0.33 +0.3 1,899,686 + BBR_RS18330 0.35 +0.8 1,899,686 + BBR_RS18330 0.35 +0.1 1,899,686 + BBR_RS18330 0.35 -0.1 1,899,686 + BBR_RS18330 0.35 +0.6 1,899,694 - BBR_RS18330 0.36 -0.3 1,899,695 - BBR_RS18330 0.36 +1.1 1,899,713 + BBR_RS18330 0.40 -1.3 1,899,713 + BBR_RS18330 0.40 +0.7 1,899,721 - BBR_RS18330 0.41 -2.5 1,899,721 - BBR_RS18330 0.41 -0.1 1,899,731 - BBR_RS18330 0.43 +0.1 1,899,731 - BBR_RS18330 0.43 -0.0 1,899,742 - BBR_RS18330 0.45 +1.0 1,899,817 + BBR_RS18330 0.58 -1.2 1,899,817 - BBR_RS18330 0.58 +0.7 1,899,825 + BBR_RS18330 0.59 +0.6 1,899,825 + BBR_RS18330 0.59 +0.9 1,899,882 + BBR_RS18330 0.69 +0.7 1,899,890 - BBR_RS18330 0.71 +0.1 1,899,890 - BBR_RS18330 0.71 -0.5 1,899,890 - BBR_RS18330 0.71 +0.2 1,899,890 - BBR_RS18330 0.71 +0.0 1,899,895 - BBR_RS18330 0.72 +0.9 1,899,921 - BBR_RS18330 0.76 +0.3 1,899,987 - BBR_RS18330 0.88 -0.5 1,899,987 - BBR_RS18330 0.88 +0.0 1,899,989 - BBR_RS18330 0.88 -0.5 1,899,990 + BBR_RS18330 0.88 -0.2 1,899,990 + BBR_RS18330 0.88 +0.1 1,899,990 + BBR_RS18330 0.88 -1.8 1,899,998 - BBR_RS18330 0.90 +1.2 1,899,998 - BBR_RS18330 0.90 -0.3 1,899,998 - BBR_RS18330 0.90 -0.1 1,899,998 - BBR_RS18330 0.90 +0.2 1,899,998 - BBR_RS18330 0.90 -0.6 1,899,998 - BBR_RS18330 0.90 +0.2 1,899,998 - BBR_RS18330 0.90 +0.5 1,899,998 - BBR_RS18330 0.90 -0.6 1,900,036 + +0.2 1,900,062 - +0.4 1,900,062 - -0.0 1,900,064 - -0.6 1,900,113 + +0.1 1,900,113 + -0.0 1,900,113 + +0.3 1,900,116 - -2.4 1,900,116 - -0.1 1,900,121 - +0.2 1,900,121 - -0.2 1,900,121 - +0.8 1,900,160 + +0.9 1,900,167 - +1.0 1,900,203 - -0.5 1,900,221 - BBR_RS18335 0.10 +0.4 1,900,240 - BBR_RS18335 0.12 -0.5 1,900,240 - BBR_RS18335 0.12 -0.2 1,900,251 + BBR_RS18335 0.13 +0.7 1,900,282 + BBR_RS18335 0.15 +0.2 1,900,331 + BBR_RS18335 0.19 -0.8 1,900,344 - BBR_RS18335 0.20 +0.4 1,900,374 - BBR_RS18335 0.22 -0.1 1,900,401 - BBR_RS18335 0.25 -0.6 1,900,413 - BBR_RS18335 0.26 +0.6 1,900,417 + BBR_RS18335 0.26 +0.6 1,900,444 + BBR_RS18335 0.28 +0.0 1,900,460 - BBR_RS18335 0.29 +0.2
Or see this region's nucleotide sequence