Experiment: Recovery broth with Chenodeoxycholic 1.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS13995 and BBR_RS14000 are separated by 27 nucleotides BBR_RS14000 and BBR_RS14005 are separated by 8 nucleotides
BBR_RS13995: BBR_RS13995 - tyrosine--tRNA ligase, at 915,987 to 917,309
_RS13995
BBR_RS14000: BBR_RS14000 - hypothetical protein, at 917,337 to 919,169
_RS14000
BBR_RS14005: BBR_RS14005 - haloacid dehalogenase, at 919,178 to 920,224
_RS14005
Position (kb)
917
918
919
920 Strain fitness (log2 ratio)
-1
0
1 at 917.663 kb on + strand, within BBR_RS14000 at 917.702 kb on + strand, within BBR_RS14000 at 917.774 kb on + strand, within BBR_RS14000 at 918.146 kb on + strand, within BBR_RS14000 at 918.826 kb on + strand, within BBR_RS14000 at 918.868 kb on + strand, within BBR_RS14000 at 918.913 kb on + strand, within BBR_RS14000 at 918.913 kb on + strand, within BBR_RS14000 at 918.913 kb on + strand, within BBR_RS14000 at 918.921 kb on - strand, within BBR_RS14000 at 918.921 kb on - strand, within BBR_RS14000 at 918.922 kb on - strand, within BBR_RS14000 at 918.932 kb on + strand, within BBR_RS14000 at 918.947 kb on + strand, within BBR_RS14000 at 918.955 kb on - strand, within BBR_RS14000 at 918.967 kb on - strand, within BBR_RS14000 at 918.979 kb on - strand, within BBR_RS14000 at 918.985 kb on + strand, within BBR_RS14000 at 918.985 kb on + strand, within BBR_RS14000 at 918.985 kb on + strand, within BBR_RS14000 at 918.993 kb on - strand at 919.015 kb on + strand at 919.072 kb on + strand at 919.072 kb on + strand at 919.142 kb on + strand at 919.171 kb on - strand at 919.196 kb on + strand at 919.253 kb on + strand at 919.253 kb on + strand at 919.255 kb on + strand at 919.261 kb on - strand at 919.292 kb on + strand, within BBR_RS14005 at 919.355 kb on + strand, within BBR_RS14005 at 919.355 kb on + strand, within BBR_RS14005 at 919.381 kb on + strand, within BBR_RS14005 at 919.436 kb on - strand, within BBR_RS14005 at 919.486 kb on + strand, within BBR_RS14005 at 919.486 kb on + strand, within BBR_RS14005 at 919.486 kb on + strand, within BBR_RS14005 at 919.486 kb on + strand, within BBR_RS14005 at 919.486 kb on + strand, within BBR_RS14005 at 919.548 kb on + strand, within BBR_RS14005 at 919.619 kb on - strand, within BBR_RS14005 at 919.621 kb on + strand, within BBR_RS14005 at 919.667 kb on + strand, within BBR_RS14005 at 919.697 kb on + strand, within BBR_RS14005 at 919.821 kb on + strand, within BBR_RS14005 at 919.946 kb on - strand, within BBR_RS14005 at 919.989 kb on + strand, within BBR_RS14005 at 920.018 kb on - strand, within BBR_RS14005 at 920.046 kb on + strand, within BBR_RS14005 at 920.046 kb on + strand, within BBR_RS14005 at 920.054 kb on - strand, within BBR_RS14005 at 920.099 kb on - strand, within BBR_RS14005 at 920.137 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Chenodeoxycholic 1.3 mM remove 917,663 + BBR_RS14000 0.18 -0.2 917,702 + BBR_RS14000 0.20 +0.6 917,774 + BBR_RS14000 0.24 -0.6 918,146 + BBR_RS14000 0.44 +0.5 918,826 + BBR_RS14000 0.81 -1.4 918,868 + BBR_RS14000 0.84 -0.2 918,913 + BBR_RS14000 0.86 -0.7 918,913 + BBR_RS14000 0.86 +0.1 918,913 + BBR_RS14000 0.86 +0.3 918,921 - BBR_RS14000 0.86 +0.2 918,921 - BBR_RS14000 0.86 +0.5 918,922 - BBR_RS14000 0.86 -0.0 918,932 + BBR_RS14000 0.87 +0.0 918,947 + BBR_RS14000 0.88 +1.3 918,955 - BBR_RS14000 0.88 -0.6 918,967 - BBR_RS14000 0.89 -1.0 918,979 - BBR_RS14000 0.90 -0.1 918,985 + BBR_RS14000 0.90 -0.3 918,985 + BBR_RS14000 0.90 -0.8 918,985 + BBR_RS14000 0.90 -0.3 918,993 - +0.8 919,015 + +1.6 919,072 + -0.4 919,072 + +0.5 919,142 + -0.7 919,171 - +0.2 919,196 + +0.4 919,253 + +1.2 919,253 + +0.8 919,255 + +0.9 919,261 - -1.2 919,292 + BBR_RS14005 0.11 +0.2 919,355 + BBR_RS14005 0.17 +0.4 919,355 + BBR_RS14005 0.17 -1.3 919,381 + BBR_RS14005 0.19 -1.0 919,436 - BBR_RS14005 0.25 +1.6 919,486 + BBR_RS14005 0.29 +0.4 919,486 + BBR_RS14005 0.29 -0.6 919,486 + BBR_RS14005 0.29 -0.1 919,486 + BBR_RS14005 0.29 +0.6 919,486 + BBR_RS14005 0.29 -1.3 919,548 + BBR_RS14005 0.35 +0.9 919,619 - BBR_RS14005 0.42 +1.1 919,621 + BBR_RS14005 0.42 +0.8 919,667 + BBR_RS14005 0.47 +0.1 919,697 + BBR_RS14005 0.50 +0.5 919,821 + BBR_RS14005 0.61 +0.8 919,946 - BBR_RS14005 0.73 -0.3 919,989 + BBR_RS14005 0.77 +0.1 920,018 - BBR_RS14005 0.80 -1.0 920,046 + BBR_RS14005 0.83 +0.6 920,046 + BBR_RS14005 0.83 +1.5 920,054 - BBR_RS14005 0.84 -0.9 920,099 - BBR_RS14005 0.88 +0.2 920,137 + -0.5
Or see this region's nucleotide sequence