Experiment: Recovery broth with Chenodeoxycholic 1.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12980 and BBR_RS12985 are separated by 171 nucleotides BBR_RS12985 and BBR_RS12990 are separated by 87 nucleotides BBR_RS12990 and BBR_RS12995 are separated by 105 nucleotides
BBR_RS12980: BBR_RS12980 - ATP-dependent DNA helicase UvrD/REP family, at 679,178 to 683,113
_RS12980
BBR_RS12985: BBR_RS12985 - MFS transporter, at 683,285 to 684,604
_RS12985
BBR_RS12990: BBR_RS12990 - 4-hydroxy-tetrahydrodipicolinate reductase, at 684,692 to 685,447
_RS12990
BBR_RS12995: BBR_RS12995 - 4-hydroxy-tetrahydrodipicolinate synthase, at 685,553 to 686,458
_RS12995
Position (kb) 683
684
685 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 682.294 kb on - strand, within BBR_RS12980 at 682.310 kb on + strand, within BBR_RS12980 at 682.328 kb on - strand, within BBR_RS12980 at 682.332 kb on - strand, within BBR_RS12980 at 682.342 kb on + strand, within BBR_RS12980 at 682.342 kb on + strand, within BBR_RS12980 at 682.345 kb on - strand, within BBR_RS12980 at 682.347 kb on + strand, within BBR_RS12980 at 682.365 kb on - strand, within BBR_RS12980 at 682.378 kb on + strand, within BBR_RS12980 at 682.396 kb on - strand, within BBR_RS12980 at 682.412 kb on - strand, within BBR_RS12980 at 682.442 kb on - strand, within BBR_RS12980 at 682.469 kb on - strand, within BBR_RS12980 at 682.470 kb on + strand, within BBR_RS12980 at 682.553 kb on + strand, within BBR_RS12980 at 682.600 kb on - strand, within BBR_RS12980 at 682.608 kb on - strand, within BBR_RS12980 at 682.624 kb on - strand, within BBR_RS12980 at 682.661 kb on + strand, within BBR_RS12980 at 682.674 kb on - strand, within BBR_RS12980 at 682.682 kb on + strand, within BBR_RS12980 at 682.698 kb on - strand, within BBR_RS12980 at 682.700 kb on + strand, within BBR_RS12980 at 682.740 kb on + strand at 682.752 kb on - strand at 682.767 kb on + strand at 682.775 kb on - strand at 682.853 kb on - strand at 682.858 kb on - strand at 682.858 kb on - strand at 682.927 kb on + strand at 682.957 kb on + strand at 682.991 kb on + strand at 683.003 kb on - strand at 683.009 kb on - strand at 683.009 kb on - strand at 683.033 kb on + strand at 683.033 kb on + strand at 683.033 kb on + strand at 683.114 kb on + strand at 683.115 kb on - strand at 683.115 kb on - strand at 683.122 kb on + strand at 683.123 kb on + strand at 683.131 kb on - strand at 683.138 kb on - strand at 683.138 kb on - strand at 683.138 kb on - strand at 683.153 kb on + strand at 683.172 kb on + strand at 683.172 kb on + strand at 683.180 kb on - strand at 683.217 kb on + strand at 683.227 kb on + strand at 683.231 kb on + strand at 683.232 kb on - strand at 683.236 kb on + strand at 683.253 kb on + strand at 683.253 kb on + strand at 683.268 kb on + strand at 683.268 kb on + strand at 683.276 kb on - strand at 683.279 kb on - strand at 683.299 kb on + strand at 683.299 kb on + strand at 683.301 kb on + strand at 683.385 kb on - strand at 683.411 kb on - strand at 683.415 kb on + strand at 683.478 kb on - strand, within BBR_RS12985 at 683.483 kb on + strand, within BBR_RS12985 at 683.503 kb on - strand, within BBR_RS12985 at 683.505 kb on + strand, within BBR_RS12985 at 683.523 kb on - strand, within BBR_RS12985 at 683.543 kb on + strand, within BBR_RS12985 at 683.604 kb on - strand, within BBR_RS12985 at 683.607 kb on + strand, within BBR_RS12985 at 683.610 kb on - strand, within BBR_RS12985 at 683.614 kb on + strand, within BBR_RS12985 at 683.663 kb on + strand, within BBR_RS12985 at 683.682 kb on - strand, within BBR_RS12985 at 683.714 kb on - strand, within BBR_RS12985 at 683.754 kb on - strand, within BBR_RS12985 at 683.771 kb on - strand, within BBR_RS12985 at 683.881 kb on + strand, within BBR_RS12985 at 683.917 kb on + strand, within BBR_RS12985 at 683.941 kb on - strand, within BBR_RS12985 at 683.965 kb on - strand, within BBR_RS12985 at 683.975 kb on - strand, within BBR_RS12985 at 684.010 kb on + strand, within BBR_RS12985 at 684.027 kb on - strand, within BBR_RS12985 at 684.039 kb on - strand, within BBR_RS12985 at 684.053 kb on + strand, within BBR_RS12985 at 684.059 kb on + strand, within BBR_RS12985 at 684.073 kb on - strand, within BBR_RS12985 at 684.074 kb on + strand, within BBR_RS12985 at 684.108 kb on + strand, within BBR_RS12985 at 684.116 kb on - strand, within BBR_RS12985 at 684.133 kb on + strand, within BBR_RS12985 at 684.147 kb on + strand, within BBR_RS12985 at 684.155 kb on - strand, within BBR_RS12985 at 684.165 kb on + strand, within BBR_RS12985 at 684.204 kb on + strand, within BBR_RS12985 at 684.283 kb on + strand, within BBR_RS12985 at 684.283 kb on + strand, within BBR_RS12985 at 684.323 kb on - strand, within BBR_RS12985 at 684.323 kb on - strand, within BBR_RS12985 at 684.341 kb on - strand, within BBR_RS12985 at 684.428 kb on + strand, within BBR_RS12985 at 684.428 kb on + strand, within BBR_RS12985 at 684.428 kb on + strand, within BBR_RS12985 at 684.431 kb on - strand, within BBR_RS12985 at 684.433 kb on + strand, within BBR_RS12985 at 684.441 kb on - strand, within BBR_RS12985 at 684.468 kb on - strand, within BBR_RS12985 at 684.497 kb on - strand at 684.497 kb on - strand at 684.550 kb on + strand at 684.550 kb on + strand at 684.550 kb on + strand at 684.552 kb on + strand at 684.558 kb on - strand at 684.573 kb on - strand at 684.584 kb on - strand at 684.599 kb on + strand at 684.599 kb on + strand at 684.607 kb on - strand at 684.607 kb on - strand at 684.694 kb on + strand at 684.735 kb on + strand at 684.749 kb on + strand at 684.749 kb on + strand at 684.803 kb on + strand, within BBR_RS12990 at 684.808 kb on + strand, within BBR_RS12990 at 684.831 kb on - strand, within BBR_RS12990 at 684.856 kb on + strand, within BBR_RS12990 at 684.874 kb on - strand, within BBR_RS12990 at 684.879 kb on - strand, within BBR_RS12990 at 684.887 kb on - strand, within BBR_RS12990 at 684.908 kb on - strand, within BBR_RS12990 at 684.925 kb on + strand, within BBR_RS12990 at 684.926 kb on - strand, within BBR_RS12990 at 684.929 kb on - strand, within BBR_RS12990 at 684.952 kb on + strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.960 kb on - strand, within BBR_RS12990 at 684.990 kb on + strand, within BBR_RS12990 at 684.997 kb on + strand, within BBR_RS12990 at 685.015 kb on - strand, within BBR_RS12990 at 685.077 kb on + strand, within BBR_RS12990 at 685.091 kb on + strand, within BBR_RS12990 at 685.096 kb on + strand, within BBR_RS12990 at 685.105 kb on + strand, within BBR_RS12990 at 685.113 kb on - strand, within BBR_RS12990 at 685.113 kb on - strand, within BBR_RS12990 at 685.123 kb on + strand, within BBR_RS12990 at 685.131 kb on - strand, within BBR_RS12990 at 685.139 kb on - strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.145 kb on + strand, within BBR_RS12990 at 685.153 kb on - strand, within BBR_RS12990 at 685.153 kb on - strand, within BBR_RS12990 at 685.153 kb on - strand, within BBR_RS12990 at 685.178 kb on - strand, within BBR_RS12990 at 685.229 kb on - strand, within BBR_RS12990 at 685.249 kb on + strand, within BBR_RS12990 at 685.257 kb on - strand, within BBR_RS12990 at 685.280 kb on - strand, within BBR_RS12990 at 685.280 kb on - strand, within BBR_RS12990 at 685.289 kb on - strand, within BBR_RS12990 at 685.297 kb on - strand, within BBR_RS12990 at 685.409 kb on + strand at 685.412 kb on - strand at 685.412 kb on - strand at 685.434 kb on + strand at 685.456 kb on - strand at 685.517 kb on + strand at 685.520 kb on + strand at 685.541 kb on + strand at 685.549 kb on + strand at 685.549 kb on + strand at 685.551 kb on + strand at 685.557 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Chenodeoxycholic 1.3 mM remove 682,294 - BBR_RS12980 0.79 -0.2 682,310 + BBR_RS12980 0.80 +0.5 682,328 - BBR_RS12980 0.80 +0.7 682,332 - BBR_RS12980 0.80 +1.3 682,342 + BBR_RS12980 0.80 +1.2 682,342 + BBR_RS12980 0.80 +0.5 682,345 - BBR_RS12980 0.80 -0.1 682,347 + BBR_RS12980 0.81 +0.8 682,365 - BBR_RS12980 0.81 -0.8 682,378 + BBR_RS12980 0.81 -2.0 682,396 - BBR_RS12980 0.82 +0.6 682,412 - BBR_RS12980 0.82 -0.5 682,442 - BBR_RS12980 0.83 +0.0 682,469 - BBR_RS12980 0.84 -1.0 682,470 + BBR_RS12980 0.84 -0.4 682,553 + BBR_RS12980 0.86 -1.6 682,600 - BBR_RS12980 0.87 -1.8 682,608 - BBR_RS12980 0.87 -1.8 682,624 - BBR_RS12980 0.88 +1.5 682,661 + BBR_RS12980 0.88 +0.0 682,674 - BBR_RS12980 0.89 -0.2 682,682 + BBR_RS12980 0.89 +0.5 682,698 - BBR_RS12980 0.89 -0.8 682,700 + BBR_RS12980 0.89 +0.1 682,740 + -1.8 682,752 - +1.2 682,767 + -0.2 682,775 - -1.3 682,853 - -1.1 682,858 - -0.3 682,858 - +0.7 682,927 + +0.5 682,957 + -0.2 682,991 + +0.5 683,003 - -1.7 683,009 - +0.9 683,009 - -0.4 683,033 + +1.2 683,033 + +0.3 683,033 + -0.5 683,114 + -0.2 683,115 - -0.8 683,115 - -0.6 683,122 + +0.0 683,123 + -2.3 683,131 - -0.0 683,138 - +0.5 683,138 - -0.3 683,138 - -0.4 683,153 + +0.1 683,172 + +0.1 683,172 + -0.6 683,180 - -2.2 683,217 + +0.2 683,227 + +0.1 683,231 + -0.4 683,232 - +0.5 683,236 + -0.4 683,253 + -0.1 683,253 + +1.3 683,268 + +0.7 683,268 + +0.3 683,276 - +0.0 683,279 - +0.6 683,299 + -0.8 683,299 + -0.1 683,301 + -1.9 683,385 - +0.0 683,411 - -0.6 683,415 + -1.5 683,478 - BBR_RS12985 0.15 -1.1 683,483 + BBR_RS12985 0.15 -2.3 683,503 - BBR_RS12985 0.17 +1.2 683,505 + BBR_RS12985 0.17 -1.8 683,523 - BBR_RS12985 0.18 +0.1 683,543 + BBR_RS12985 0.20 -1.0 683,604 - BBR_RS12985 0.24 -1.0 683,607 + BBR_RS12985 0.24 -0.5 683,610 - BBR_RS12985 0.25 +0.5 683,614 + BBR_RS12985 0.25 -0.2 683,663 + BBR_RS12985 0.29 -0.5 683,682 - BBR_RS12985 0.30 -1.9 683,714 - BBR_RS12985 0.33 -1.8 683,754 - BBR_RS12985 0.36 +1.8 683,771 - BBR_RS12985 0.37 -1.4 683,881 + BBR_RS12985 0.45 +0.3 683,917 + BBR_RS12985 0.48 -1.5 683,941 - BBR_RS12985 0.50 -1.1 683,965 - BBR_RS12985 0.52 -0.0 683,975 - BBR_RS12985 0.52 -0.7 684,010 + BBR_RS12985 0.55 -1.5 684,027 - BBR_RS12985 0.56 -0.5 684,039 - BBR_RS12985 0.57 -0.8 684,053 + BBR_RS12985 0.58 -1.3 684,059 + BBR_RS12985 0.59 +0.4 684,073 - BBR_RS12985 0.60 -1.1 684,074 + BBR_RS12985 0.60 +1.2 684,108 + BBR_RS12985 0.62 -0.3 684,116 - BBR_RS12985 0.63 +1.5 684,133 + BBR_RS12985 0.64 -0.2 684,147 + BBR_RS12985 0.65 -0.3 684,155 - BBR_RS12985 0.66 -0.1 684,165 + BBR_RS12985 0.67 -2.7 684,204 + BBR_RS12985 0.70 -1.0 684,283 + BBR_RS12985 0.76 +0.4 684,283 + BBR_RS12985 0.76 -1.0 684,323 - BBR_RS12985 0.79 -0.2 684,323 - BBR_RS12985 0.79 -1.1 684,341 - BBR_RS12985 0.80 -2.5 684,428 + BBR_RS12985 0.87 -1.0 684,428 + BBR_RS12985 0.87 +2.1 684,428 + BBR_RS12985 0.87 +0.8 684,431 - BBR_RS12985 0.87 -1.3 684,433 + BBR_RS12985 0.87 +1.4 684,441 - BBR_RS12985 0.88 -2.7 684,468 - BBR_RS12985 0.90 -1.7 684,497 - +0.5 684,497 - +0.4 684,550 + +0.3 684,550 + -1.2 684,550 + -2.3 684,552 + -0.7 684,558 - -0.1 684,573 - -1.4 684,584 - -1.5 684,599 + +0.8 684,599 + -1.6 684,607 - +0.3 684,607 - -1.6 684,694 + -0.1 684,735 + -0.3 684,749 + +0.3 684,749 + -0.1 684,803 + BBR_RS12990 0.15 +1.0 684,808 + BBR_RS12990 0.15 -0.9 684,831 - BBR_RS12990 0.18 -0.4 684,856 + BBR_RS12990 0.22 -0.2 684,874 - BBR_RS12990 0.24 -1.0 684,879 - BBR_RS12990 0.25 +0.5 684,887 - BBR_RS12990 0.26 +0.3 684,908 - BBR_RS12990 0.29 -0.4 684,925 + BBR_RS12990 0.31 -0.7 684,926 - BBR_RS12990 0.31 +0.1 684,929 - BBR_RS12990 0.31 +0.6 684,952 + BBR_RS12990 0.34 +0.4 684,960 - BBR_RS12990 0.35 -0.7 684,960 - BBR_RS12990 0.35 +0.1 684,960 - BBR_RS12990 0.35 -0.3 684,960 - BBR_RS12990 0.35 +0.1 684,990 + BBR_RS12990 0.39 +2.8 684,997 + BBR_RS12990 0.40 -0.8 685,015 - BBR_RS12990 0.43 +0.3 685,077 + BBR_RS12990 0.51 -1.7 685,091 + BBR_RS12990 0.53 -0.7 685,096 + BBR_RS12990 0.53 -0.3 685,105 + BBR_RS12990 0.55 +0.8 685,113 - BBR_RS12990 0.56 +0.4 685,113 - BBR_RS12990 0.56 +0.5 685,123 + BBR_RS12990 0.57 -0.1 685,131 - BBR_RS12990 0.58 +0.1 685,139 - BBR_RS12990 0.59 +0.1 685,145 + BBR_RS12990 0.60 -0.4 685,145 + BBR_RS12990 0.60 +1.1 685,145 + BBR_RS12990 0.60 +0.4 685,145 + BBR_RS12990 0.60 -0.2 685,145 + BBR_RS12990 0.60 -0.4 685,153 - BBR_RS12990 0.61 -2.1 685,153 - BBR_RS12990 0.61 -0.7 685,153 - BBR_RS12990 0.61 -1.0 685,178 - BBR_RS12990 0.64 +0.2 685,229 - BBR_RS12990 0.71 -0.0 685,249 + BBR_RS12990 0.74 +0.6 685,257 - BBR_RS12990 0.75 -0.5 685,280 - BBR_RS12990 0.78 +0.5 685,280 - BBR_RS12990 0.78 +0.5 685,289 - BBR_RS12990 0.79 +0.2 685,297 - BBR_RS12990 0.80 -1.7 685,409 + +1.0 685,412 - +0.9 685,412 - +1.1 685,434 + +1.1 685,456 - -2.2 685,517 + +0.9 685,520 + +0.1 685,541 + +0.2 685,549 + +0.0 685,549 + +1.0 685,551 + +0.8 685,557 - +0.9
Or see this region's nucleotide sequence