Experiment: Recovery broth with Chenodeoxycholic 1.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12920 and BBR_RS12925 are separated by 181 nucleotides BBR_RS12925 and BBR_RS12930 are separated by 273 nucleotides BBR_RS12930 and BBR_RS12935 are separated by 20 nucleotides
BBR_RS12920: BBR_RS12920 - glycine synthesis protein GlyXL (from data) , at 650,899 to 652,263
_RS12920
BBR_RS12925: BBR_RS12925 - hypothetical protein, at 652,445 to 652,648
_RS12925
BBR_RS12930: BBR_RS12930 - tRNA (cytidine(34)-2'-O)-methyltransferase, at 652,922 to 653,584
_RS12930
BBR_RS12935: BBR_RS12935 - A/G-specific adenine glycosylase, at 653,605 to 654,567
_RS12935
Position (kb)
652
653 Strain fitness (log2 ratio)
-2
-1
0
1 at 651.482 kb on + strand, within BBR_RS12920 at 651.484 kb on + strand, within BBR_RS12920 at 651.504 kb on + strand, within BBR_RS12920 at 651.511 kb on + strand, within BBR_RS12920 at 651.526 kb on + strand, within BBR_RS12920 at 651.526 kb on + strand, within BBR_RS12920 at 651.526 kb on + strand, within BBR_RS12920 at 651.526 kb on + strand, within BBR_RS12920 at 651.529 kb on - strand, within BBR_RS12920 at 651.594 kb on + strand, within BBR_RS12920 at 651.594 kb on + strand, within BBR_RS12920 at 651.601 kb on + strand, within BBR_RS12920 at 651.601 kb on + strand, within BBR_RS12920 at 651.634 kb on - strand, within BBR_RS12920 at 651.645 kb on + strand, within BBR_RS12920 at 651.669 kb on + strand, within BBR_RS12920 at 651.669 kb on + strand, within BBR_RS12920 at 651.742 kb on - strand, within BBR_RS12920 at 651.785 kb on + strand, within BBR_RS12920 at 651.785 kb on + strand, within BBR_RS12920 at 651.814 kb on + strand, within BBR_RS12920 at 651.819 kb on + strand, within BBR_RS12920 at 651.828 kb on - strand, within BBR_RS12920 at 651.843 kb on + strand, within BBR_RS12920 at 651.860 kb on - strand, within BBR_RS12920 at 651.865 kb on + strand, within BBR_RS12920 at 651.865 kb on - strand, within BBR_RS12920 at 651.869 kb on + strand, within BBR_RS12920 at 651.934 kb on - strand, within BBR_RS12920 at 651.949 kb on + strand, within BBR_RS12920 at 651.950 kb on + strand, within BBR_RS12920 at 651.950 kb on + strand, within BBR_RS12920 at 651.951 kb on + strand, within BBR_RS12920 at 651.958 kb on + strand, within BBR_RS12920 at 651.958 kb on + strand, within BBR_RS12920 at 651.966 kb on + strand, within BBR_RS12920 at 651.966 kb on + strand, within BBR_RS12920 at 651.966 kb on + strand, within BBR_RS12920 at 651.973 kb on + strand, within BBR_RS12920 at 651.980 kb on - strand, within BBR_RS12920 at 651.981 kb on - strand, within BBR_RS12920 at 651.981 kb on - strand, within BBR_RS12920 at 651.991 kb on - strand, within BBR_RS12920 at 651.998 kb on + strand, within BBR_RS12920 at 652.028 kb on + strand, within BBR_RS12920 at 652.033 kb on + strand, within BBR_RS12920 at 652.043 kb on + strand, within BBR_RS12920 at 652.069 kb on + strand, within BBR_RS12920 at 652.087 kb on - strand, within BBR_RS12920 at 652.089 kb on + strand, within BBR_RS12920 at 652.092 kb on + strand, within BBR_RS12920 at 652.094 kb on + strand, within BBR_RS12920 at 652.113 kb on - strand, within BBR_RS12920 at 652.130 kb on - strand at 652.156 kb on + strand at 652.156 kb on + strand at 652.199 kb on + strand at 652.201 kb on + strand at 652.201 kb on + strand at 652.262 kb on + strand at 652.273 kb on + strand at 652.375 kb on + strand at 652.493 kb on + strand, within BBR_RS12925 at 652.561 kb on - strand, within BBR_RS12925 at 652.638 kb on + strand at 652.658 kb on - strand at 652.743 kb on + strand at 652.743 kb on - strand at 652.743 kb on - strand at 652.744 kb on - strand at 652.749 kb on - strand at 652.830 kb on - strand at 652.845 kb on - strand at 652.849 kb on - strand at 652.849 kb on - strand at 652.913 kb on + strand at 652.949 kb on + strand at 652.953 kb on - strand at 652.957 kb on - strand at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.990 kb on + strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 652.998 kb on - strand, within BBR_RS12930 at 653.011 kb on + strand, within BBR_RS12930 at 653.013 kb on + strand, within BBR_RS12930 at 653.032 kb on + strand, within BBR_RS12930 at 653.068 kb on + strand, within BBR_RS12930 at 653.086 kb on + strand, within BBR_RS12930 at 653.096 kb on + strand, within BBR_RS12930 at 653.096 kb on + strand, within BBR_RS12930 at 653.135 kb on + strand, within BBR_RS12930 at 653.157 kb on - strand, within BBR_RS12930 at 653.187 kb on + strand, within BBR_RS12930 at 653.199 kb on - strand, within BBR_RS12930 at 653.204 kb on - strand, within BBR_RS12930 at 653.204 kb on - strand, within BBR_RS12930 at 653.223 kb on + strand, within BBR_RS12930 at 653.225 kb on - strand, within BBR_RS12930 at 653.235 kb on - strand, within BBR_RS12930 at 653.235 kb on - strand, within BBR_RS12930 at 653.242 kb on + strand, within BBR_RS12930 at 653.243 kb on + strand, within BBR_RS12930 at 653.251 kb on - strand, within BBR_RS12930 at 653.263 kb on + strand, within BBR_RS12930 at 653.271 kb on - strand, within BBR_RS12930 at 653.276 kb on - strand, within BBR_RS12930 at 653.278 kb on + strand, within BBR_RS12930 at 653.286 kb on - strand, within BBR_RS12930 at 653.286 kb on - strand, within BBR_RS12930 at 653.286 kb on - strand, within BBR_RS12930 at 653.286 kb on - strand, within BBR_RS12930 at 653.286 kb on - strand, within BBR_RS12930 at 653.286 kb on - strand, within BBR_RS12930 at 653.368 kb on + strand, within BBR_RS12930 at 653.370 kb on + strand, within BBR_RS12930 at 653.371 kb on - strand, within BBR_RS12930 at 653.378 kb on + strand, within BBR_RS12930 at 653.378 kb on - strand, within BBR_RS12930 at 653.389 kb on + strand, within BBR_RS12930 at 653.399 kb on - strand, within BBR_RS12930 at 653.421 kb on + strand, within BBR_RS12930 at 653.429 kb on + strand, within BBR_RS12930 at 653.451 kb on + strand, within BBR_RS12930 at 653.459 kb on - strand, within BBR_RS12930 at 653.483 kb on - strand, within BBR_RS12930 at 653.602 kb on - strand at 653.605 kb on + strand at 653.639 kb on + strand at 653.639 kb on + strand at 653.639 kb on - strand at 653.643 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Chenodeoxycholic 1.3 mM remove 651,482 + BBR_RS12920 0.43 +0.2 651,484 + BBR_RS12920 0.43 -0.6 651,504 + BBR_RS12920 0.44 -1.6 651,511 + BBR_RS12920 0.45 -0.8 651,526 + BBR_RS12920 0.46 -0.1 651,526 + BBR_RS12920 0.46 +0.9 651,526 + BBR_RS12920 0.46 -0.6 651,526 + BBR_RS12920 0.46 -0.1 651,529 - BBR_RS12920 0.46 +0.3 651,594 + BBR_RS12920 0.51 +0.0 651,594 + BBR_RS12920 0.51 -0.6 651,601 + BBR_RS12920 0.51 +0.1 651,601 + BBR_RS12920 0.51 +0.8 651,634 - BBR_RS12920 0.54 -1.1 651,645 + BBR_RS12920 0.55 -0.0 651,669 + BBR_RS12920 0.56 -0.5 651,669 + BBR_RS12920 0.56 -0.3 651,742 - BBR_RS12920 0.62 +0.1 651,785 + BBR_RS12920 0.65 -0.5 651,785 + BBR_RS12920 0.65 -0.4 651,814 + BBR_RS12920 0.67 -0.2 651,819 + BBR_RS12920 0.67 +0.8 651,828 - BBR_RS12920 0.68 +0.7 651,843 + BBR_RS12920 0.69 +0.2 651,860 - BBR_RS12920 0.70 +1.5 651,865 + BBR_RS12920 0.71 +0.3 651,865 - BBR_RS12920 0.71 -0.0 651,869 + BBR_RS12920 0.71 -0.3 651,934 - BBR_RS12920 0.76 +0.1 651,949 + BBR_RS12920 0.77 -0.2 651,950 + BBR_RS12920 0.77 +0.3 651,950 + BBR_RS12920 0.77 -0.9 651,951 + BBR_RS12920 0.77 +0.5 651,958 + BBR_RS12920 0.78 -0.1 651,958 + BBR_RS12920 0.78 -0.1 651,966 + BBR_RS12920 0.78 -0.7 651,966 + BBR_RS12920 0.78 +0.2 651,966 + BBR_RS12920 0.78 -1.0 651,973 + BBR_RS12920 0.79 -0.2 651,980 - BBR_RS12920 0.79 -0.9 651,981 - BBR_RS12920 0.79 +0.9 651,981 - BBR_RS12920 0.79 +0.1 651,991 - BBR_RS12920 0.80 +0.6 651,998 + BBR_RS12920 0.81 +0.8 652,028 + BBR_RS12920 0.83 +0.1 652,033 + BBR_RS12920 0.83 -0.2 652,043 + BBR_RS12920 0.84 -0.0 652,069 + BBR_RS12920 0.86 +0.0 652,087 - BBR_RS12920 0.87 +0.3 652,089 + BBR_RS12920 0.87 +0.4 652,092 + BBR_RS12920 0.87 -0.9 652,094 + BBR_RS12920 0.88 -0.7 652,113 - BBR_RS12920 0.89 -0.0 652,130 - -1.6 652,156 + -0.2 652,156 + -1.1 652,199 + -0.7 652,201 + -1.0 652,201 + -1.3 652,262 + +0.9 652,273 + +0.2 652,375 + -0.4 652,493 + BBR_RS12925 0.24 -0.3 652,561 - BBR_RS12925 0.57 -0.2 652,638 + -0.6 652,658 - -0.1 652,743 + +0.2 652,743 - -1.0 652,743 - -0.5 652,744 - +0.9 652,749 - +1.0 652,830 - -0.0 652,845 - +0.6 652,849 - +0.4 652,849 - -0.2 652,913 + -0.5 652,949 + +0.2 652,953 - +0.7 652,957 - -0.5 652,990 + BBR_RS12930 0.10 +1.5 652,990 + BBR_RS12930 0.10 -0.7 652,990 + BBR_RS12930 0.10 +0.0 652,990 + BBR_RS12930 0.10 -0.2 652,990 + BBR_RS12930 0.10 -0.8 652,990 + BBR_RS12930 0.10 -1.3 652,990 + BBR_RS12930 0.10 +0.9 652,990 + BBR_RS12930 0.10 -0.4 652,998 - BBR_RS12930 0.11 -0.7 652,998 - BBR_RS12930 0.11 -1.5 652,998 - BBR_RS12930 0.11 +0.5 652,998 - BBR_RS12930 0.11 +0.3 652,998 - BBR_RS12930 0.11 -0.3 652,998 - BBR_RS12930 0.11 -0.3 652,998 - BBR_RS12930 0.11 +0.7 652,998 - BBR_RS12930 0.11 -0.8 652,998 - BBR_RS12930 0.11 +0.9 652,998 - BBR_RS12930 0.11 +0.9 652,998 - BBR_RS12930 0.11 +1.3 652,998 - BBR_RS12930 0.11 +0.6 652,998 - BBR_RS12930 0.11 +0.3 653,011 + BBR_RS12930 0.13 -0.7 653,013 + BBR_RS12930 0.14 +0.3 653,032 + BBR_RS12930 0.17 +0.5 653,068 + BBR_RS12930 0.22 -1.1 653,086 + BBR_RS12930 0.25 -0.1 653,096 + BBR_RS12930 0.26 +0.6 653,096 + BBR_RS12930 0.26 +0.9 653,135 + BBR_RS12930 0.32 +0.2 653,157 - BBR_RS12930 0.35 +0.4 653,187 + BBR_RS12930 0.40 -1.9 653,199 - BBR_RS12930 0.42 +0.4 653,204 - BBR_RS12930 0.43 +0.4 653,204 - BBR_RS12930 0.43 -0.7 653,223 + BBR_RS12930 0.45 +0.3 653,225 - BBR_RS12930 0.46 -0.2 653,235 - BBR_RS12930 0.47 +0.6 653,235 - BBR_RS12930 0.47 -0.3 653,242 + BBR_RS12930 0.48 +1.4 653,243 + BBR_RS12930 0.48 -0.1 653,251 - BBR_RS12930 0.50 -0.1 653,263 + BBR_RS12930 0.51 +0.6 653,271 - BBR_RS12930 0.53 -0.3 653,276 - BBR_RS12930 0.53 +0.6 653,278 + BBR_RS12930 0.54 +1.0 653,286 - BBR_RS12930 0.55 -0.5 653,286 - BBR_RS12930 0.55 -0.5 653,286 - BBR_RS12930 0.55 -0.2 653,286 - BBR_RS12930 0.55 +0.5 653,286 - BBR_RS12930 0.55 -0.0 653,286 - BBR_RS12930 0.55 +1.0 653,368 + BBR_RS12930 0.67 +0.2 653,370 + BBR_RS12930 0.68 +0.1 653,371 - BBR_RS12930 0.68 +1.4 653,378 + BBR_RS12930 0.69 +0.2 653,378 - BBR_RS12930 0.69 +0.6 653,389 + BBR_RS12930 0.70 +0.4 653,399 - BBR_RS12930 0.72 +1.5 653,421 + BBR_RS12930 0.75 -0.1 653,429 + BBR_RS12930 0.76 -1.3 653,451 + BBR_RS12930 0.80 +1.4 653,459 - BBR_RS12930 0.81 -0.5 653,483 - BBR_RS12930 0.85 +0.9 653,602 - -0.6 653,605 + -0.3 653,639 + +1.4 653,639 + +0.4 653,639 - +0.8 653,643 + -0.6
Or see this region's nucleotide sequence