Experiment: Recovery broth with Chenodeoxycholic 1.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS12850 and BBR_RS12855 are separated by 15 nucleotides BBR_RS12855 and BBR_RS12860 are separated by 46 nucleotides BBR_RS12860 and BBR_RS12865 are separated by 22 nucleotides
BBR_RS12850: BBR_RS12850 - NAD(+) diphosphatase, at 632,039 to 633,295
_RS12850
BBR_RS12855: BBR_RS12855 - glycine cleavage system protein H, at 633,311 to 633,712
_RS12855
BBR_RS12860: BBR_RS12860 - DUF2183 domain-containing protein, at 633,759 to 635,015
_RS12860
BBR_RS12865: BBR_RS12865 - S9 family peptidase, at 635,038 to 637,794
_RS12865
Position (kb) 633
634
635
636 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 632.766 kb on + strand, within BBR_RS12850 at 632.766 kb on + strand, within BBR_RS12850 at 632.774 kb on - strand, within BBR_RS12850 at 632.790 kb on - strand, within BBR_RS12850 at 632.807 kb on + strand, within BBR_RS12850 at 632.809 kb on - strand, within BBR_RS12850 at 632.836 kb on + strand, within BBR_RS12850 at 632.844 kb on - strand, within BBR_RS12850 at 632.849 kb on - strand, within BBR_RS12850 at 632.849 kb on - strand, within BBR_RS12850 at 632.884 kb on - strand, within BBR_RS12850 at 632.889 kb on - strand, within BBR_RS12850 at 632.933 kb on + strand, within BBR_RS12850 at 632.933 kb on + strand, within BBR_RS12850 at 632.938 kb on + strand, within BBR_RS12850 at 632.938 kb on + strand, within BBR_RS12850 at 632.941 kb on - strand, within BBR_RS12850 at 632.941 kb on - strand, within BBR_RS12850 at 632.979 kb on + strand, within BBR_RS12850 at 633.008 kb on - strand, within BBR_RS12850 at 633.048 kb on + strand, within BBR_RS12850 at 633.048 kb on + strand, within BBR_RS12850 at 633.048 kb on + strand, within BBR_RS12850 at 633.065 kb on + strand, within BBR_RS12850 at 633.070 kb on + strand, within BBR_RS12850 at 633.071 kb on - strand, within BBR_RS12850 at 633.078 kb on - strand, within BBR_RS12850 at 633.078 kb on - strand, within BBR_RS12850 at 633.116 kb on + strand, within BBR_RS12850 at 633.126 kb on - strand, within BBR_RS12850 at 633.126 kb on - strand, within BBR_RS12850 at 633.149 kb on + strand, within BBR_RS12850 at 633.149 kb on + strand, within BBR_RS12850 at 633.197 kb on + strand at 633.239 kb on + strand at 633.259 kb on + strand at 633.259 kb on + strand at 633.259 kb on + strand at 633.259 kb on + strand at 633.264 kb on + strand at 633.267 kb on - strand at 633.267 kb on - strand at 633.267 kb on - strand at 633.267 kb on - strand at 633.267 kb on - strand at 633.267 kb on - strand at 633.267 kb on - strand at 633.267 kb on - strand at 633.272 kb on - strand at 633.284 kb on - strand at 633.288 kb on + strand at 633.288 kb on + strand at 633.288 kb on + strand at 633.290 kb on + strand at 633.295 kb on + strand at 633.298 kb on - strand at 633.298 kb on - strand at 633.299 kb on + strand at 633.307 kb on + strand at 633.307 kb on + strand at 633.315 kb on - strand at 633.315 kb on - strand at 633.329 kb on - strand at 633.329 kb on - strand at 633.356 kb on - strand, within BBR_RS12855 at 633.357 kb on + strand, within BBR_RS12855 at 633.359 kb on + strand, within BBR_RS12855 at 633.361 kb on + strand, within BBR_RS12855 at 633.379 kb on + strand, within BBR_RS12855 at 633.379 kb on + strand, within BBR_RS12855 at 633.379 kb on + strand, within BBR_RS12855 at 633.379 kb on + strand, within BBR_RS12855 at 633.379 kb on + strand, within BBR_RS12855 at 633.564 kb on - strand, within BBR_RS12855 at 633.648 kb on - strand, within BBR_RS12855 at 633.697 kb on - strand at 633.707 kb on - strand at 633.727 kb on - strand at 633.733 kb on + strand at 633.736 kb on - strand at 633.742 kb on + strand at 633.747 kb on + strand at 633.751 kb on - strand at 633.805 kb on + strand at 633.818 kb on - strand at 633.875 kb on + strand at 633.901 kb on + strand, within BBR_RS12860 at 633.904 kb on - strand, within BBR_RS12860 at 633.910 kb on + strand, within BBR_RS12860 at 633.911 kb on + strand, within BBR_RS12860 at 633.918 kb on - strand, within BBR_RS12860 at 633.919 kb on - strand, within BBR_RS12860 at 633.927 kb on + strand, within BBR_RS12860 at 633.946 kb on + strand, within BBR_RS12860 at 633.951 kb on + strand, within BBR_RS12860 at 633.954 kb on - strand, within BBR_RS12860 at 633.964 kb on - strand, within BBR_RS12860 at 634.024 kb on - strand, within BBR_RS12860 at 634.031 kb on + strand, within BBR_RS12860 at 634.035 kb on + strand, within BBR_RS12860 at 634.039 kb on - strand, within BBR_RS12860 at 634.049 kb on - strand, within BBR_RS12860 at 634.049 kb on - strand, within BBR_RS12860 at 634.118 kb on + strand, within BBR_RS12860 at 634.162 kb on + strand, within BBR_RS12860 at 634.170 kb on - strand, within BBR_RS12860 at 634.185 kb on - strand, within BBR_RS12860 at 634.191 kb on + strand, within BBR_RS12860 at 634.191 kb on + strand, within BBR_RS12860 at 634.244 kb on - strand, within BBR_RS12860 at 634.246 kb on + strand, within BBR_RS12860 at 634.246 kb on + strand, within BBR_RS12860 at 634.254 kb on - strand, within BBR_RS12860 at 634.272 kb on + strand, within BBR_RS12860 at 634.272 kb on - strand, within BBR_RS12860 at 634.291 kb on + strand, within BBR_RS12860 at 634.299 kb on - strand, within BBR_RS12860 at 634.299 kb on - strand, within BBR_RS12860 at 634.315 kb on + strand, within BBR_RS12860 at 634.338 kb on + strand, within BBR_RS12860 at 634.379 kb on + strand, within BBR_RS12860 at 634.379 kb on + strand, within BBR_RS12860 at 634.379 kb on + strand, within BBR_RS12860 at 634.379 kb on + strand, within BBR_RS12860 at 634.387 kb on - strand, within BBR_RS12860 at 634.387 kb on - strand, within BBR_RS12860 at 634.387 kb on - strand, within BBR_RS12860 at 634.390 kb on - strand, within BBR_RS12860 at 634.397 kb on - strand, within BBR_RS12860 at 634.397 kb on - strand, within BBR_RS12860 at 634.399 kb on + strand, within BBR_RS12860 at 634.407 kb on - strand, within BBR_RS12860 at 634.407 kb on - strand, within BBR_RS12860 at 634.407 kb on - strand, within BBR_RS12860 at 634.415 kb on + strand, within BBR_RS12860 at 634.425 kb on - strand, within BBR_RS12860 at 634.435 kb on - strand, within BBR_RS12860 at 634.471 kb on - strand, within BBR_RS12860 at 634.528 kb on - strand, within BBR_RS12860 at 634.528 kb on - strand, within BBR_RS12860 at 634.533 kb on + strand, within BBR_RS12860 at 634.546 kb on + strand, within BBR_RS12860 at 634.556 kb on + strand, within BBR_RS12860 at 634.666 kb on - strand, within BBR_RS12860 at 634.666 kb on - strand, within BBR_RS12860 at 634.669 kb on + strand, within BBR_RS12860 at 634.669 kb on + strand, within BBR_RS12860 at 634.669 kb on + strand, within BBR_RS12860 at 634.677 kb on - strand, within BBR_RS12860 at 634.677 kb on - strand, within BBR_RS12860 at 634.677 kb on - strand, within BBR_RS12860 at 634.677 kb on - strand, within BBR_RS12860 at 634.698 kb on - strand, within BBR_RS12860 at 634.699 kb on + strand, within BBR_RS12860 at 634.729 kb on - strand, within BBR_RS12860 at 634.729 kb on - strand, within BBR_RS12860 at 634.736 kb on + strand, within BBR_RS12860 at 634.744 kb on - strand, within BBR_RS12860 at 634.744 kb on - strand, within BBR_RS12860 at 634.836 kb on - strand, within BBR_RS12860 at 634.909 kb on + strand at 635.009 kb on + strand at 635.034 kb on - strand at 635.042 kb on + strand at 635.043 kb on - strand at 635.077 kb on + strand at 635.077 kb on + strand at 635.103 kb on - strand at 635.103 kb on - strand at 635.125 kb on - strand at 635.132 kb on + strand at 635.170 kb on - strand at 635.254 kb on - strand at 635.264 kb on - strand at 635.281 kb on + strand at 635.298 kb on - strand at 635.327 kb on + strand, within BBR_RS12865 at 635.341 kb on + strand, within BBR_RS12865 at 635.346 kb on + strand, within BBR_RS12865 at 635.350 kb on + strand, within BBR_RS12865 at 635.351 kb on - strand, within BBR_RS12865 at 635.351 kb on - strand, within BBR_RS12865 at 635.360 kb on + strand, within BBR_RS12865 at 635.369 kb on - strand, within BBR_RS12865 at 635.409 kb on + strand, within BBR_RS12865 at 635.416 kb on + strand, within BBR_RS12865 at 635.417 kb on - strand, within BBR_RS12865 at 635.532 kb on + strand, within BBR_RS12865 at 635.538 kb on - strand, within BBR_RS12865 at 635.540 kb on + strand, within BBR_RS12865 at 635.578 kb on + strand, within BBR_RS12865 at 635.614 kb on + strand, within BBR_RS12865 at 635.634 kb on + strand, within BBR_RS12865 at 635.734 kb on - strand, within BBR_RS12865 at 635.754 kb on + strand, within BBR_RS12865 at 635.760 kb on - strand, within BBR_RS12865 at 635.785 kb on - strand, within BBR_RS12865 at 635.800 kb on - strand, within BBR_RS12865 at 635.800 kb on - strand, within BBR_RS12865 at 635.842 kb on + strand, within BBR_RS12865 at 635.857 kb on - strand, within BBR_RS12865 at 635.915 kb on + strand, within BBR_RS12865 at 635.923 kb on - strand, within BBR_RS12865 at 635.941 kb on - strand, within BBR_RS12865 at 635.994 kb on - strand, within BBR_RS12865
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Chenodeoxycholic 1.3 mM remove 632,766 + BBR_RS12850 0.58 +0.2 632,766 + BBR_RS12850 0.58 +0.7 632,774 - BBR_RS12850 0.58 -0.4 632,790 - BBR_RS12850 0.60 -0.1 632,807 + BBR_RS12850 0.61 -2.3 632,809 - BBR_RS12850 0.61 +0.4 632,836 + BBR_RS12850 0.63 +0.6 632,844 - BBR_RS12850 0.64 +0.1 632,849 - BBR_RS12850 0.64 +0.6 632,849 - BBR_RS12850 0.64 +0.6 632,884 - BBR_RS12850 0.67 -0.6 632,889 - BBR_RS12850 0.68 +0.7 632,933 + BBR_RS12850 0.71 -0.9 632,933 + BBR_RS12850 0.71 -0.5 632,938 + BBR_RS12850 0.72 +1.3 632,938 + BBR_RS12850 0.72 -0.7 632,941 - BBR_RS12850 0.72 +0.5 632,941 - BBR_RS12850 0.72 +0.8 632,979 + BBR_RS12850 0.75 +0.3 633,008 - BBR_RS12850 0.77 -2.7 633,048 + BBR_RS12850 0.80 -0.2 633,048 + BBR_RS12850 0.80 +0.5 633,048 + BBR_RS12850 0.80 -0.1 633,065 + BBR_RS12850 0.82 -0.3 633,070 + BBR_RS12850 0.82 -0.9 633,071 - BBR_RS12850 0.82 -0.2 633,078 - BBR_RS12850 0.83 -0.6 633,078 - BBR_RS12850 0.83 -0.6 633,116 + BBR_RS12850 0.86 -1.5 633,126 - BBR_RS12850 0.86 +0.1 633,126 - BBR_RS12850 0.86 -0.8 633,149 + BBR_RS12850 0.88 +0.3 633,149 + BBR_RS12850 0.88 +0.2 633,197 + -0.0 633,239 + -0.6 633,259 + -0.3 633,259 + +0.3 633,259 + +1.2 633,259 + +1.5 633,264 + -0.2 633,267 - -0.5 633,267 - +0.5 633,267 - -0.0 633,267 - -1.8 633,267 - -0.3 633,267 - +1.3 633,267 - +0.3 633,267 - -0.2 633,272 - -0.1 633,284 - +1.1 633,288 + -1.1 633,288 + -0.3 633,288 + +0.3 633,290 + -1.0 633,295 + +0.7 633,298 - +0.6 633,298 - +0.4 633,299 + -0.2 633,307 + +1.8 633,307 + +1.2 633,315 - +0.3 633,315 - +0.6 633,329 - +0.2 633,329 - +0.4 633,356 - BBR_RS12855 0.11 +0.8 633,357 + BBR_RS12855 0.11 -0.3 633,359 + BBR_RS12855 0.12 +0.5 633,361 + BBR_RS12855 0.12 +0.0 633,379 + BBR_RS12855 0.17 +0.3 633,379 + BBR_RS12855 0.17 +0.1 633,379 + BBR_RS12855 0.17 -0.7 633,379 + BBR_RS12855 0.17 -0.1 633,379 + BBR_RS12855 0.17 +0.7 633,564 - BBR_RS12855 0.63 -0.1 633,648 - BBR_RS12855 0.84 +0.7 633,697 - -0.2 633,707 - +0.1 633,727 - +0.4 633,733 + +1.7 633,736 - +0.4 633,742 + -0.1 633,747 + +1.5 633,751 - +0.7 633,805 + +0.6 633,818 - +1.3 633,875 + -2.0 633,901 + BBR_RS12860 0.11 -0.0 633,904 - BBR_RS12860 0.12 -0.9 633,910 + BBR_RS12860 0.12 -0.4 633,911 + BBR_RS12860 0.12 -0.7 633,918 - BBR_RS12860 0.13 -1.4 633,919 - BBR_RS12860 0.13 +0.1 633,927 + BBR_RS12860 0.13 -0.9 633,946 + BBR_RS12860 0.15 -0.1 633,951 + BBR_RS12860 0.15 -1.5 633,954 - BBR_RS12860 0.16 -1.6 633,964 - BBR_RS12860 0.16 -1.3 634,024 - BBR_RS12860 0.21 -1.6 634,031 + BBR_RS12860 0.22 -2.6 634,035 + BBR_RS12860 0.22 -1.7 634,039 - BBR_RS12860 0.22 -1.4 634,049 - BBR_RS12860 0.23 +0.2 634,049 - BBR_RS12860 0.23 -1.8 634,118 + BBR_RS12860 0.29 -3.5 634,162 + BBR_RS12860 0.32 -1.0 634,170 - BBR_RS12860 0.33 +1.1 634,185 - BBR_RS12860 0.34 +1.2 634,191 + BBR_RS12860 0.34 -0.6 634,191 + BBR_RS12860 0.34 -1.0 634,244 - BBR_RS12860 0.39 -0.3 634,246 + BBR_RS12860 0.39 -0.6 634,246 + BBR_RS12860 0.39 -0.6 634,254 - BBR_RS12860 0.39 -0.0 634,272 + BBR_RS12860 0.41 -0.9 634,272 - BBR_RS12860 0.41 -2.9 634,291 + BBR_RS12860 0.42 -1.3 634,299 - BBR_RS12860 0.43 -1.1 634,299 - BBR_RS12860 0.43 +0.3 634,315 + BBR_RS12860 0.44 -1.4 634,338 + BBR_RS12860 0.46 -1.3 634,379 + BBR_RS12860 0.49 -2.1 634,379 + BBR_RS12860 0.49 -1.8 634,379 + BBR_RS12860 0.49 -0.5 634,379 + BBR_RS12860 0.49 -1.3 634,387 - BBR_RS12860 0.50 +1.5 634,387 - BBR_RS12860 0.50 -1.5 634,387 - BBR_RS12860 0.50 -0.2 634,390 - BBR_RS12860 0.50 -1.6 634,397 - BBR_RS12860 0.51 -1.2 634,397 - BBR_RS12860 0.51 -0.3 634,399 + BBR_RS12860 0.51 -0.4 634,407 - BBR_RS12860 0.52 -1.0 634,407 - BBR_RS12860 0.52 -1.3 634,407 - BBR_RS12860 0.52 -1.8 634,415 + BBR_RS12860 0.52 -1.9 634,425 - BBR_RS12860 0.53 -1.8 634,435 - BBR_RS12860 0.54 +0.8 634,471 - BBR_RS12860 0.57 -1.0 634,528 - BBR_RS12860 0.61 +0.5 634,528 - BBR_RS12860 0.61 -1.2 634,533 + BBR_RS12860 0.62 -1.6 634,546 + BBR_RS12860 0.63 +0.1 634,556 + BBR_RS12860 0.63 -1.2 634,666 - BBR_RS12860 0.72 +1.1 634,666 - BBR_RS12860 0.72 +1.1 634,669 + BBR_RS12860 0.72 +0.5 634,669 + BBR_RS12860 0.72 -1.6 634,669 + BBR_RS12860 0.72 +0.3 634,677 - BBR_RS12860 0.73 -1.9 634,677 - BBR_RS12860 0.73 -0.2 634,677 - BBR_RS12860 0.73 -0.2 634,677 - BBR_RS12860 0.73 -0.6 634,698 - BBR_RS12860 0.75 -2.5 634,699 + BBR_RS12860 0.75 -0.8 634,729 - BBR_RS12860 0.77 -2.0 634,729 - BBR_RS12860 0.77 -1.2 634,736 + BBR_RS12860 0.78 +0.8 634,744 - BBR_RS12860 0.78 -1.6 634,744 - BBR_RS12860 0.78 -0.7 634,836 - BBR_RS12860 0.86 -1.0 634,909 + -0.5 635,009 + -0.7 635,034 - +2.7 635,042 + +0.4 635,043 - -0.1 635,077 + +0.8 635,077 + +0.5 635,103 - -1.9 635,103 - +1.1 635,125 - -1.1 635,132 + -1.2 635,170 - -0.5 635,254 - -0.2 635,264 - +0.6 635,281 + -0.1 635,298 - +0.1 635,327 + BBR_RS12865 0.10 +0.0 635,341 + BBR_RS12865 0.11 -0.4 635,346 + BBR_RS12865 0.11 +0.2 635,350 + BBR_RS12865 0.11 -0.4 635,351 - BBR_RS12865 0.11 -0.9 635,351 - BBR_RS12865 0.11 +0.3 635,360 + BBR_RS12865 0.12 +1.8 635,369 - BBR_RS12865 0.12 -0.5 635,409 + BBR_RS12865 0.13 +0.8 635,416 + BBR_RS12865 0.14 -0.0 635,417 - BBR_RS12865 0.14 -1.2 635,532 + BBR_RS12865 0.18 +0.2 635,538 - BBR_RS12865 0.18 +0.5 635,540 + BBR_RS12865 0.18 -1.4 635,578 + BBR_RS12865 0.20 +0.2 635,614 + BBR_RS12865 0.21 +1.4 635,634 + BBR_RS12865 0.22 +0.1 635,734 - BBR_RS12865 0.25 -1.0 635,754 + BBR_RS12865 0.26 -1.1 635,760 - BBR_RS12865 0.26 -1.2 635,785 - BBR_RS12865 0.27 +0.1 635,800 - BBR_RS12865 0.28 -0.4 635,800 - BBR_RS12865 0.28 -1.1 635,842 + BBR_RS12865 0.29 -0.7 635,857 - BBR_RS12865 0.30 -1.8 635,915 + BBR_RS12865 0.32 -0.2 635,923 - BBR_RS12865 0.32 -0.9 635,941 - BBR_RS12865 0.33 -0.9 635,994 - BBR_RS12865 0.35 -0.5
Or see this region's nucleotide sequence