Experiment: Recovery broth with Chenodeoxycholic 1.3 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS10900 and BBR_RS10905 overlap by 1 nucleotides BBR_RS10905 and BBR_RS10910 are separated by 178 nucleotides BBR_RS10910 and BBR_RS10915 are separated by 155 nucleotides
BBR_RS10900: BBR_RS10900 - molecular chaperone DnaK, at 188,855 to 190,735
_RS10900
BBR_RS10905: BBR_RS10905 - nucleotide exchange factor GrpE, at 190,735 to 191,418
_RS10905
BBR_RS10910: BBR_RS10910 - molecular chaperone DnaJ, at 191,597 to 192,610
_RS10910
BBR_RS10915: BBR_RS10915 - MerR family DNA-binding transcriptional regulator, at 192,766 to 193,353
_RS10915
Position (kb) 191
192
193 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 190.599 kb on + strand at 190.599 kb on + strand at 190.611 kb on + strand at 190.611 kb on + strand at 190.815 kb on + strand, within BBR_RS10905 at 190.815 kb on + strand, within BBR_RS10905 at 190.845 kb on + strand, within BBR_RS10905 at 190.845 kb on + strand, within BBR_RS10905 at 190.845 kb on + strand, within BBR_RS10905 at 190.860 kb on + strand, within BBR_RS10905 at 190.860 kb on + strand, within BBR_RS10905 at 190.998 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.022 kb on + strand, within BBR_RS10905 at 191.108 kb on + strand, within BBR_RS10905 at 191.305 kb on + strand, within BBR_RS10905 at 191.390 kb on + strand at 191.395 kb on + strand at 191.422 kb on + strand at 191.425 kb on - strand at 191.425 kb on - strand at 191.433 kb on + strand at 191.472 kb on - strand at 191.478 kb on + strand at 191.478 kb on + strand at 191.484 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.488 kb on + strand at 191.496 kb on - strand at 191.496 kb on - strand at 191.496 kb on - strand at 191.499 kb on + strand at 191.499 kb on + strand at 191.507 kb on - strand at 191.519 kb on - strand at 191.527 kb on + strand at 191.535 kb on - strand at 191.543 kb on - strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.545 kb on + strand at 191.553 kb on - strand at 191.553 kb on - strand at 191.567 kb on + strand at 191.587 kb on + strand at 191.603 kb on + strand at 191.608 kb on - strand at 191.619 kb on + strand at 191.626 kb on + strand at 191.626 kb on + strand at 191.636 kb on + strand at 191.647 kb on + strand at 191.647 kb on + strand at 191.647 kb on + strand at 191.655 kb on - strand at 191.655 kb on - strand at 191.664 kb on - strand at 191.701 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.704 kb on + strand, within BBR_RS10910 at 191.709 kb on + strand, within BBR_RS10910 at 191.709 kb on + strand, within BBR_RS10910 at 191.709 kb on + strand, within BBR_RS10910 at 191.710 kb on + strand, within BBR_RS10910 at 191.712 kb on - strand, within BBR_RS10910 at 191.719 kb on + strand, within BBR_RS10910 at 191.746 kb on + strand, within BBR_RS10910 at 191.757 kb on + strand, within BBR_RS10910 at 191.765 kb on + strand, within BBR_RS10910 at 191.777 kb on - strand, within BBR_RS10910 at 191.782 kb on - strand, within BBR_RS10910 at 191.784 kb on + strand, within BBR_RS10910 at 191.825 kb on + strand, within BBR_RS10910 at 191.829 kb on - strand, within BBR_RS10910 at 191.861 kb on + strand, within BBR_RS10910 at 191.877 kb on + strand, within BBR_RS10910 at 191.886 kb on + strand, within BBR_RS10910 at 191.896 kb on + strand, within BBR_RS10910 at 191.925 kb on - strand, within BBR_RS10910 at 191.970 kb on + strand, within BBR_RS10910 at 191.970 kb on + strand, within BBR_RS10910 at 191.971 kb on + strand, within BBR_RS10910 at 191.998 kb on + strand, within BBR_RS10910 at 192.014 kb on + strand, within BBR_RS10910 at 192.016 kb on + strand, within BBR_RS10910 at 192.043 kb on + strand, within BBR_RS10910 at 192.044 kb on + strand, within BBR_RS10910 at 192.049 kb on + strand, within BBR_RS10910 at 192.049 kb on + strand, within BBR_RS10910 at 192.049 kb on + strand, within BBR_RS10910 at 192.049 kb on + strand, within BBR_RS10910 at 192.057 kb on - strand, within BBR_RS10910 at 192.069 kb on + strand, within BBR_RS10910 at 192.080 kb on + strand, within BBR_RS10910 at 192.104 kb on + strand, within BBR_RS10910 at 192.106 kb on + strand, within BBR_RS10910 at 192.123 kb on + strand, within BBR_RS10910 at 192.133 kb on + strand, within BBR_RS10910 at 192.142 kb on + strand, within BBR_RS10910 at 192.142 kb on + strand, within BBR_RS10910 at 192.142 kb on + strand, within BBR_RS10910 at 192.148 kb on + strand, within BBR_RS10910 at 192.160 kb on + strand, within BBR_RS10910 at 192.184 kb on + strand, within BBR_RS10910 at 192.184 kb on + strand, within BBR_RS10910 at 192.192 kb on - strand, within BBR_RS10910 at 192.192 kb on - strand, within BBR_RS10910 at 192.192 kb on - strand, within BBR_RS10910 at 192.192 kb on - strand, within BBR_RS10910 at 192.226 kb on + strand, within BBR_RS10910 at 192.226 kb on + strand, within BBR_RS10910 at 192.244 kb on + strand, within BBR_RS10910 at 192.252 kb on - strand, within BBR_RS10910 at 192.330 kb on + strand, within BBR_RS10910 at 192.341 kb on + strand, within BBR_RS10910 at 192.341 kb on + strand, within BBR_RS10910 at 192.341 kb on + strand, within BBR_RS10910 at 192.341 kb on + strand, within BBR_RS10910 at 192.355 kb on + strand, within BBR_RS10910 at 192.360 kb on + strand, within BBR_RS10910 at 192.360 kb on + strand, within BBR_RS10910 at 192.360 kb on + strand, within BBR_RS10910 at 192.360 kb on + strand, within BBR_RS10910 at 192.364 kb on + strand, within BBR_RS10910 at 192.365 kb on + strand, within BBR_RS10910 at 192.368 kb on - strand, within BBR_RS10910 at 192.368 kb on - strand, within BBR_RS10910 at 192.368 kb on - strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.388 kb on + strand, within BBR_RS10910 at 192.400 kb on + strand, within BBR_RS10910 at 192.423 kb on + strand, within BBR_RS10910 at 192.423 kb on + strand, within BBR_RS10910 at 192.423 kb on + strand, within BBR_RS10910 at 192.423 kb on + strand, within BBR_RS10910 at 192.423 kb on + strand, within BBR_RS10910 at 192.423 kb on + strand, within BBR_RS10910 at 192.423 kb on + strand, within BBR_RS10910 at 192.424 kb on - strand, within BBR_RS10910 at 192.431 kb on - strand, within BBR_RS10910 at 192.431 kb on - strand, within BBR_RS10910 at 192.448 kb on - strand, within BBR_RS10910 at 192.450 kb on - strand, within BBR_RS10910 at 192.460 kb on + strand, within BBR_RS10910 at 192.460 kb on + strand, within BBR_RS10910 at 192.481 kb on - strand, within BBR_RS10910 at 192.511 kb on + strand at 192.516 kb on + strand at 192.524 kb on - strand at 192.526 kb on + strand at 192.535 kb on - strand at 192.557 kb on + strand at 192.565 kb on - strand at 192.606 kb on + strand at 192.631 kb on + strand at 192.641 kb on + strand at 192.646 kb on + strand at 192.654 kb on - strand at 192.661 kb on + strand at 192.661 kb on + strand at 192.661 kb on + strand at 192.661 kb on + strand at 192.675 kb on + strand at 192.675 kb on + strand at 192.683 kb on - strand at 192.683 kb on - strand at 192.693 kb on - strand at 192.695 kb on + strand at 192.695 kb on + strand at 192.710 kb on + strand at 192.710 kb on + strand at 192.710 kb on + strand at 192.717 kb on - strand at 192.717 kb on - strand at 192.718 kb on - strand at 192.756 kb on + strand at 192.789 kb on - strand at 192.798 kb on + strand at 192.828 kb on + strand, within BBR_RS10915 at 192.924 kb on + strand, within BBR_RS10915 at 192.944 kb on - strand, within BBR_RS10915 at 192.993 kb on + strand, within BBR_RS10915 at 192.995 kb on + strand, within BBR_RS10915 at 193.042 kb on + strand, within BBR_RS10915 at 193.055 kb on + strand, within BBR_RS10915 at 193.123 kb on + strand, within BBR_RS10915 at 193.123 kb on + strand, within BBR_RS10915 at 193.131 kb on + strand, within BBR_RS10915 at 193.144 kb on + strand, within BBR_RS10915 at 193.181 kb on + strand, within BBR_RS10915 at 193.189 kb on - strand, within BBR_RS10915 at 193.191 kb on - strand, within BBR_RS10915 at 193.224 kb on - strand, within BBR_RS10915 at 193.228 kb on + strand, within BBR_RS10915 at 193.244 kb on + strand, within BBR_RS10915 at 193.254 kb on + strand, within BBR_RS10915 at 193.260 kb on - strand, within BBR_RS10915 at 193.302 kb on + strand at 193.308 kb on + strand at 193.308 kb on + strand at 193.310 kb on - strand at 193.310 kb on - strand at 193.316 kb on - strand at 193.323 kb on + strand at 193.323 kb on - strand at 193.323 kb on - strand at 193.329 kb on + strand at 193.329 kb on - strand at 193.329 kb on - strand at 193.331 kb on + strand at 193.331 kb on - strand at 193.336 kb on + strand at 193.337 kb on - strand at 193.338 kb on + strand at 193.338 kb on + strand at 193.344 kb on + strand at 193.344 kb on - strand at 193.344 kb on - strand at 193.346 kb on - strand at 193.346 kb on - strand at 193.454 kb on + strand at 193.454 kb on + strand at 193.459 kb on + strand at 193.477 kb on + strand at 193.500 kb on + strand at 193.500 kb on + strand at 193.500 kb on + strand at 193.500 kb on + strand at 193.500 kb on + strand at 193.500 kb on + strand at 193.500 kb on + strand at 193.500 kb on + strand at 193.500 kb on + strand at 193.500 kb on + strand at 193.500 kb on + strand at 193.508 kb on - strand at 193.508 kb on - strand at 193.508 kb on - strand at 193.508 kb on - strand at 193.508 kb on - strand at 193.508 kb on - strand at 193.508 kb on - strand at 193.520 kb on + strand at 193.520 kb on + strand at 193.521 kb on + strand at 193.528 kb on - strand at 193.528 kb on - strand at 193.528 kb on - strand at 193.528 kb on - strand at 193.528 kb on - strand at 193.530 kb on - strand at 193.536 kb on - strand at 193.545 kb on - strand at 193.551 kb on + strand at 193.553 kb on + strand at 193.561 kb on - strand at 193.561 kb on - strand at 193.579 kb on + strand at 193.579 kb on + strand at 193.579 kb on + strand at 193.579 kb on - strand at 193.587 kb on - strand at 193.587 kb on - strand at 193.587 kb on - strand at 193.587 kb on - strand at 193.587 kb on - strand at 193.587 kb on - strand at 193.587 kb on - strand at 193.592 kb on - strand at 193.592 kb on - strand at 193.599 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Chenodeoxycholic 1.3 mM remove 190,599 + +2.9 190,599 + -0.7 190,611 + +1.3 190,611 + +0.6 190,815 + BBR_RS10905 0.12 -0.7 190,815 + BBR_RS10905 0.12 -1.0 190,845 + BBR_RS10905 0.16 -1.2 190,845 + BBR_RS10905 0.16 -1.6 190,845 + BBR_RS10905 0.16 -1.1 190,860 + BBR_RS10905 0.18 -2.6 190,860 + BBR_RS10905 0.18 -0.4 190,998 + BBR_RS10905 0.38 -1.3 191,022 + BBR_RS10905 0.42 -1.0 191,022 + BBR_RS10905 0.42 -2.5 191,022 + BBR_RS10905 0.42 -1.4 191,022 + BBR_RS10905 0.42 +0.6 191,108 + BBR_RS10905 0.55 +1.9 191,305 + BBR_RS10905 0.83 +0.4 191,390 + +0.3 191,395 + +2.9 191,422 + +0.0 191,425 - +0.8 191,425 - +0.4 191,433 + +0.1 191,472 - -0.8 191,478 + +0.3 191,478 + -2.1 191,484 + -1.0 191,488 + -0.0 191,488 + +0.0 191,488 + +0.7 191,488 + +0.0 191,488 + +0.2 191,496 - +0.9 191,496 - -0.6 191,496 - -2.4 191,499 + +0.8 191,499 + -1.3 191,507 - -0.6 191,519 - -0.8 191,527 + +0.1 191,535 - -0.5 191,543 - +0.2 191,545 + +2.0 191,545 + -1.2 191,545 + +0.3 191,545 + +0.8 191,545 + +0.3 191,553 - +0.9 191,553 - -1.4 191,567 + +0.2 191,587 + +1.1 191,603 + -1.2 191,608 - +0.4 191,619 + +1.9 191,626 + -1.0 191,626 + -0.6 191,636 + +1.2 191,647 + -0.3 191,647 + +0.2 191,647 + -1.3 191,655 - +0.3 191,655 - +0.6 191,664 - -0.0 191,701 + BBR_RS10910 0.10 -1.3 191,704 + BBR_RS10910 0.11 +0.6 191,704 + BBR_RS10910 0.11 -1.4 191,704 + BBR_RS10910 0.11 -0.2 191,704 + BBR_RS10910 0.11 +0.4 191,704 + BBR_RS10910 0.11 +1.9 191,704 + BBR_RS10910 0.11 -0.5 191,709 + BBR_RS10910 0.11 -0.4 191,709 + BBR_RS10910 0.11 +0.8 191,709 + BBR_RS10910 0.11 -1.6 191,710 + BBR_RS10910 0.11 -0.1 191,712 - BBR_RS10910 0.11 +0.9 191,719 + BBR_RS10910 0.12 -0.9 191,746 + BBR_RS10910 0.15 -0.4 191,757 + BBR_RS10910 0.16 -1.1 191,765 + BBR_RS10910 0.17 -1.0 191,777 - BBR_RS10910 0.18 +1.1 191,782 - BBR_RS10910 0.18 +0.9 191,784 + BBR_RS10910 0.18 +0.6 191,825 + BBR_RS10910 0.22 +0.2 191,829 - BBR_RS10910 0.23 +0.6 191,861 + BBR_RS10910 0.26 -1.2 191,877 + BBR_RS10910 0.28 +0.1 191,886 + BBR_RS10910 0.29 -1.1 191,896 + BBR_RS10910 0.29 -0.1 191,925 - BBR_RS10910 0.32 +1.9 191,970 + BBR_RS10910 0.37 +0.3 191,970 + BBR_RS10910 0.37 -1.4 191,971 + BBR_RS10910 0.37 +1.4 191,998 + BBR_RS10910 0.40 +1.3 192,014 + BBR_RS10910 0.41 -0.0 192,016 + BBR_RS10910 0.41 -1.8 192,043 + BBR_RS10910 0.44 -1.9 192,044 + BBR_RS10910 0.44 +1.7 192,049 + BBR_RS10910 0.45 +0.2 192,049 + BBR_RS10910 0.45 -3.6 192,049 + BBR_RS10910 0.45 +1.7 192,049 + BBR_RS10910 0.45 +0.2 192,057 - BBR_RS10910 0.45 -0.3 192,069 + BBR_RS10910 0.47 +0.1 192,080 + BBR_RS10910 0.48 -3.1 192,104 + BBR_RS10910 0.50 -2.5 192,106 + BBR_RS10910 0.50 -0.4 192,123 + BBR_RS10910 0.52 -2.0 192,133 + BBR_RS10910 0.53 -0.1 192,142 + BBR_RS10910 0.54 -0.5 192,142 + BBR_RS10910 0.54 -0.1 192,142 + BBR_RS10910 0.54 +0.4 192,148 + BBR_RS10910 0.54 +0.5 192,160 + BBR_RS10910 0.56 +0.9 192,184 + BBR_RS10910 0.58 -0.1 192,184 + BBR_RS10910 0.58 -1.3 192,192 - BBR_RS10910 0.59 -1.6 192,192 - BBR_RS10910 0.59 +1.2 192,192 - BBR_RS10910 0.59 -0.2 192,192 - BBR_RS10910 0.59 +0.6 192,226 + BBR_RS10910 0.62 -0.3 192,226 + BBR_RS10910 0.62 -1.0 192,244 + BBR_RS10910 0.64 -0.0 192,252 - BBR_RS10910 0.65 +1.3 192,330 + BBR_RS10910 0.72 -0.9 192,341 + BBR_RS10910 0.73 -2.4 192,341 + BBR_RS10910 0.73 +0.4 192,341 + BBR_RS10910 0.73 -0.7 192,341 + BBR_RS10910 0.73 -0.0 192,355 + BBR_RS10910 0.75 -2.3 192,360 + BBR_RS10910 0.75 +0.3 192,360 + BBR_RS10910 0.75 -0.7 192,360 + BBR_RS10910 0.75 -0.3 192,360 + BBR_RS10910 0.75 -0.8 192,364 + BBR_RS10910 0.76 -0.1 192,365 + BBR_RS10910 0.76 -0.6 192,368 - BBR_RS10910 0.76 -0.7 192,368 - BBR_RS10910 0.76 -0.4 192,368 - BBR_RS10910 0.76 +0.7 192,388 + BBR_RS10910 0.78 -1.1 192,388 + BBR_RS10910 0.78 -0.2 192,388 + BBR_RS10910 0.78 +0.2 192,388 + BBR_RS10910 0.78 +0.3 192,388 + BBR_RS10910 0.78 -0.7 192,388 + BBR_RS10910 0.78 +1.0 192,388 + BBR_RS10910 0.78 +0.3 192,400 + BBR_RS10910 0.79 +0.7 192,423 + BBR_RS10910 0.81 +0.1 192,423 + BBR_RS10910 0.81 +1.7 192,423 + BBR_RS10910 0.81 -1.4 192,423 + BBR_RS10910 0.81 -1.4 192,423 + BBR_RS10910 0.81 -0.1 192,423 + BBR_RS10910 0.81 -1.2 192,423 + BBR_RS10910 0.81 -0.4 192,424 - BBR_RS10910 0.82 +1.6 192,431 - BBR_RS10910 0.82 -0.7 192,431 - BBR_RS10910 0.82 +0.1 192,448 - BBR_RS10910 0.84 +1.9 192,450 - BBR_RS10910 0.84 -1.2 192,460 + BBR_RS10910 0.85 +0.2 192,460 + BBR_RS10910 0.85 -0.6 192,481 - BBR_RS10910 0.87 -0.5 192,511 + -0.2 192,516 + +0.8 192,524 - +0.1 192,526 + -0.8 192,535 - -0.4 192,557 + -0.4 192,565 - +0.2 192,606 + -0.7 192,631 + +0.2 192,641 + -0.8 192,646 + +1.5 192,654 - -2.5 192,661 + +0.7 192,661 + +0.7 192,661 + +0.6 192,661 + -0.1 192,675 + +1.1 192,675 + -0.3 192,683 - +0.7 192,683 - +0.5 192,693 - +0.5 192,695 + -1.8 192,695 + +1.3 192,710 + -1.8 192,710 + +0.3 192,710 + +0.7 192,717 - -0.8 192,717 - +0.5 192,718 - -0.8 192,756 + +1.1 192,789 - +1.5 192,798 + -0.4 192,828 + BBR_RS10915 0.11 -0.4 192,924 + BBR_RS10915 0.27 -0.2 192,944 - BBR_RS10915 0.30 +1.9 192,993 + BBR_RS10915 0.39 +1.1 192,995 + BBR_RS10915 0.39 -0.1 193,042 + BBR_RS10915 0.47 +0.1 193,055 + BBR_RS10915 0.49 -1.6 193,123 + BBR_RS10915 0.61 +0.3 193,123 + BBR_RS10915 0.61 +0.8 193,131 + BBR_RS10915 0.62 +0.7 193,144 + BBR_RS10915 0.64 -1.7 193,181 + BBR_RS10915 0.71 -1.4 193,189 - BBR_RS10915 0.72 -0.3 193,191 - BBR_RS10915 0.72 +0.3 193,224 - BBR_RS10915 0.78 -0.6 193,228 + BBR_RS10915 0.79 -3.4 193,244 + BBR_RS10915 0.81 +1.1 193,254 + BBR_RS10915 0.83 -0.0 193,260 - BBR_RS10915 0.84 +0.4 193,302 + +0.1 193,308 + -0.7 193,308 + -0.6 193,310 - +2.9 193,310 - -0.3 193,316 - -0.7 193,323 + -0.2 193,323 - -0.0 193,323 - -0.9 193,329 + +0.2 193,329 - -0.9 193,329 - +0.5 193,331 + +1.0 193,331 - +0.3 193,336 + +0.3 193,337 - -0.1 193,338 + +0.6 193,338 + +0.1 193,344 + -0.2 193,344 - +1.3 193,344 - +0.7 193,346 - +1.1 193,346 - +0.8 193,454 + -0.5 193,454 + +0.6 193,459 + +0.6 193,477 + +0.0 193,500 + -0.8 193,500 + +0.3 193,500 + +0.8 193,500 + -0.4 193,500 + +0.3 193,500 + +0.5 193,500 + -0.7 193,500 + +0.1 193,500 + -0.4 193,500 + -0.6 193,500 + +0.5 193,508 - +0.6 193,508 - +0.5 193,508 - +0.6 193,508 - +1.2 193,508 - +0.6 193,508 - +1.3 193,508 - +0.1 193,520 + -0.9 193,520 + -1.0 193,521 + +0.0 193,528 - -0.2 193,528 - +0.2 193,528 - +0.9 193,528 - +0.7 193,528 - -0.1 193,530 - -0.8 193,536 - -1.7 193,545 - -0.3 193,551 + -1.6 193,553 + +1.0 193,561 - +0.1 193,561 - -0.3 193,579 + +0.2 193,579 + -0.1 193,579 + -0.3 193,579 - +1.1 193,587 - -0.3 193,587 - +0.4 193,587 - +0.6 193,587 - +1.1 193,587 - +1.4 193,587 - -0.4 193,587 - +0.4 193,592 - -0.5 193,592 - -0.9 193,599 + +0.2
Or see this region's nucleotide sequence