Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS23240

Experiment: D-Fructose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS23235 and BWI76_RS23240 are separated by 128 nucleotidesBWI76_RS23240 and BWI76_RS23245 are separated by 12 nucleotidesBWI76_RS23245 and BWI76_RS23250 overlap by 16 nucleotidesBWI76_RS23250 and BWI76_RS23255 overlap by 4 nucleotides BWI76_RS23235: BWI76_RS23235 - pitrilysin, at 4,843,827 to 4,846,712 _RS23235 BWI76_RS23240: BWI76_RS23240 - exonuclease V subunit gamma, at 4,846,841 to 4,850,215 _RS23240 BWI76_RS23245: BWI76_RS23245 - hypothetical protein, at 4,850,228 to 4,850,551 _RS23245 BWI76_RS23250: BWI76_RS23250 - hypothetical protein, at 4,850,536 to 4,850,943 _RS23250 BWI76_RS23255: BWI76_RS23255 - prepilin-type cleavage/methylation domain-containing protein, at 4,850,940 to 4,851,503 _RS23255 Position (kb) 4846 4847 4848 4849 4850 4851Strain fitness (log2 ratio) -2 -1 0 1at 4846.713 kb on + strandat 4847.810 kb on + strand, within BWI76_RS23240at 4848.002 kb on - strand, within BWI76_RS23240at 4848.397 kb on + strand, within BWI76_RS23240at 4848.798 kb on + strand, within BWI76_RS23240at 4848.932 kb on + strand, within BWI76_RS23240at 4848.940 kb on - strand, within BWI76_RS23240at 4849.704 kb on + strand, within BWI76_RS23240at 4849.712 kb on - strand, within BWI76_RS23240at 4850.375 kb on - strand, within BWI76_RS23245at 4850.433 kb on + strand, within BWI76_RS23245at 4850.679 kb on - strand, within BWI76_RS23250at 4850.784 kb on - strand, within BWI76_RS23250at 4850.953 kb on - strandat 4851.173 kb on + strand, within BWI76_RS23255

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Fructose (C)
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4,846,713 + +0.3
4,847,810 + BWI76_RS23240 0.29 -1.6
4,848,002 - BWI76_RS23240 0.34 -2.0
4,848,397 + BWI76_RS23240 0.46 -1.0
4,848,798 + BWI76_RS23240 0.58 -2.4
4,848,932 + BWI76_RS23240 0.62 -1.0
4,848,940 - BWI76_RS23240 0.62 -2.5
4,849,704 + BWI76_RS23240 0.85 -2.3
4,849,712 - BWI76_RS23240 0.85 -1.6
4,850,375 - BWI76_RS23245 0.45 -1.7
4,850,433 + BWI76_RS23245 0.63 +0.3
4,850,679 - BWI76_RS23250 0.35 +0.6
4,850,784 - BWI76_RS23250 0.61 +0.0
4,850,953 - +1.6
4,851,173 + BWI76_RS23255 0.41 -0.5

Or see this region's nucleotide sequence