Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS00990

Experiment: D-Fructose (C)

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS00985 and BWI76_RS00990 are separated by 163 nucleotidesBWI76_RS00990 and BWI76_RS00995 are separated by 100 nucleotidesBWI76_RS00995 and BWI76_RS01000 are separated by 52 nucleotidesBWI76_RS01000 and BWI76_RS01005 are separated by 194 nucleotides BWI76_RS00985: BWI76_RS00985 - transcriptional regulator IlvY, at 201,750 to 202,640 _RS00985 BWI76_RS00990: BWI76_RS00990 - ketol-acid reductoisomerase, at 202,804 to 204,279 _RS00990 BWI76_RS00995: BWI76_RS00995 - peptidyl-prolyl cis-trans isomerase, at 204,380 to 204,661 _RS00995 BWI76_RS01000: BWI76_RS01000 - peptidyl-prolyl cis-trans isomerase, at 204,714 to 204,995 _RS01000 BWI76_RS01005: BWI76_RS01005 - CarD family transcriptional regulator, at 205,190 to 205,894 _RS01005 Position (kb) 202 203 204 205Strain fitness (log2 ratio) -5 -4 -3 -2 -1 0 1 2 3at 201.918 kb on - strand, within BWI76_RS00985at 201.928 kb on - strand, within BWI76_RS00985at 201.974 kb on + strand, within BWI76_RS00985at 202.030 kb on - strand, within BWI76_RS00985at 202.043 kb on - strand, within BWI76_RS00985at 202.101 kb on - strand, within BWI76_RS00985at 202.114 kb on + strand, within BWI76_RS00985at 202.114 kb on + strand, within BWI76_RS00985at 202.177 kb on + strand, within BWI76_RS00985at 202.264 kb on + strand, within BWI76_RS00985at 202.444 kb on + strand, within BWI76_RS00985at 202.515 kb on + strand, within BWI76_RS00985at 202.560 kb on + strandat 202.610 kb on + strandat 202.625 kb on - strandat 202.796 kb on + strandat 202.862 kb on - strandat 202.960 kb on + strand, within BWI76_RS00990at 202.968 kb on - strand, within BWI76_RS00990at 202.971 kb on + strand, within BWI76_RS00990at 202.976 kb on + strand, within BWI76_RS00990at 203.048 kb on - strand, within BWI76_RS00990at 203.182 kb on + strand, within BWI76_RS00990at 203.379 kb on + strand, within BWI76_RS00990at 203.617 kb on - strand, within BWI76_RS00990at 203.617 kb on - strand, within BWI76_RS00990at 203.709 kb on + strand, within BWI76_RS00990at 204.075 kb on - strandat 204.167 kb on + strandat 204.305 kb on + strandat 204.306 kb on + strandat 204.330 kb on + strandat 204.458 kb on + strand, within BWI76_RS00995at 204.466 kb on - strand, within BWI76_RS00995at 204.496 kb on - strand, within BWI76_RS00995at 204.720 kb on - strandat 204.783 kb on - strand, within BWI76_RS01000at 204.800 kb on - strandat 204.819 kb on + strand, within BWI76_RS01000at 204.906 kb on + strand, within BWI76_RS01000at 204.936 kb on - strand, within BWI76_RS01000at 204.948 kb on + strand, within BWI76_RS01000at 204.977 kb on - strandat 204.977 kb on - strandat 205.053 kb on + strandat 205.053 kb on + strandat 205.145 kb on + strandat 205.163 kb on + strandat 205.163 kb on + strandat 205.165 kb on + strandat 205.165 kb on + strandat 205.166 kb on - strandat 205.240 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Fructose (C)
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201,918 - BWI76_RS00985 0.19 -1.0
201,928 - BWI76_RS00985 0.20 -1.6
201,974 + BWI76_RS00985 0.25 -1.7
202,030 - BWI76_RS00985 0.31 -3.2
202,043 - BWI76_RS00985 0.33 -4.2
202,101 - BWI76_RS00985 0.39 -1.5
202,114 + BWI76_RS00985 0.41 -1.3
202,114 + BWI76_RS00985 0.41 -3.1
202,177 + BWI76_RS00985 0.48 -4.6
202,264 + BWI76_RS00985 0.58 -2.8
202,444 + BWI76_RS00985 0.78 -3.7
202,515 + BWI76_RS00985 0.86 -2.4
202,560 + -1.1
202,610 + -2.0
202,625 - -3.5
202,796 + -2.7
202,862 - -4.1
202,960 + BWI76_RS00990 0.11 +1.8
202,968 - BWI76_RS00990 0.11 -5.5
202,971 + BWI76_RS00990 0.11 -3.9
202,976 + BWI76_RS00990 0.12 -5.7
203,048 - BWI76_RS00990 0.17 -1.7
203,182 + BWI76_RS00990 0.26 -5.3
203,379 + BWI76_RS00990 0.39 -3.6
203,617 - BWI76_RS00990 0.55 -1.0
203,617 - BWI76_RS00990 0.55 -3.5
203,709 + BWI76_RS00990 0.61 -1.7
204,075 - +0.2
204,167 + -3.3
204,305 + +0.2
204,306 + +1.4
204,330 + +0.3
204,458 + BWI76_RS00995 0.28 -0.3
204,466 - BWI76_RS00995 0.30 +0.3
204,496 - BWI76_RS00995 0.41 +0.9
204,720 - +0.1
204,783 - BWI76_RS01000 0.24 -0.0
204,800 - +0.8
204,819 + BWI76_RS01000 0.37 -0.5
204,906 + BWI76_RS01000 0.68 +0.6
204,936 - BWI76_RS01000 0.79 +0.4
204,948 + BWI76_RS01000 0.83 +1.2
204,977 - -0.6
204,977 - +0.8
205,053 + +0.7
205,053 + -0.1
205,145 + +1.8
205,163 + +2.8
205,163 + +1.1
205,165 + +0.2
205,165 + +0.2
205,166 - +0.0
205,240 + +0.5

Or see this region's nucleotide sequence