Experiment: Recovery broth with Chenodeoxycholic 2.5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BBR_RS14320 and BBR_RS14325 are separated by 42 nucleotides BBR_RS14325 and BBR_RS14330 are separated by 73 nucleotides
BBR_RS14320: BBR_RS14320 - Nif3-like dinuclear metal center hexameric protein, at 989,045 to 989,965
_RS14320
BBR_RS14325: BBR_RS14325 - NUDIX hydrolase, at 990,008 to 990,682
_RS14325
BBR_RS14330: BBR_RS14330 - glycogen debranching enzyme GlgX, at 990,756 to 992,867
_RS14330
Position (kb) 990
991 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 989.034 kb on - strand at 989.035 kb on + strand at 989.043 kb on - strand at 989.080 kb on - strand at 989.115 kb on - strand at 989.183 kb on + strand, within BBR_RS14320 at 989.205 kb on - strand, within BBR_RS14320 at 989.235 kb on + strand, within BBR_RS14320 at 989.307 kb on + strand, within BBR_RS14320 at 989.315 kb on - strand, within BBR_RS14320 at 989.322 kb on + strand, within BBR_RS14320 at 989.370 kb on + strand, within BBR_RS14320 at 989.375 kb on + strand, within BBR_RS14320 at 989.378 kb on - strand, within BBR_RS14320 at 989.380 kb on + strand, within BBR_RS14320 at 989.400 kb on + strand, within BBR_RS14320 at 989.426 kb on - strand, within BBR_RS14320 at 989.455 kb on + strand, within BBR_RS14320 at 989.539 kb on + strand, within BBR_RS14320 at 989.551 kb on - strand, within BBR_RS14320 at 989.601 kb on + strand, within BBR_RS14320 at 989.645 kb on - strand, within BBR_RS14320 at 989.666 kb on + strand, within BBR_RS14320 at 989.689 kb on + strand, within BBR_RS14320 at 989.745 kb on + strand, within BBR_RS14320 at 989.753 kb on - strand, within BBR_RS14320 at 989.757 kb on + strand, within BBR_RS14320 at 989.759 kb on - strand, within BBR_RS14320 at 989.791 kb on - strand, within BBR_RS14320 at 989.811 kb on - strand, within BBR_RS14320 at 989.850 kb on + strand, within BBR_RS14320 at 989.850 kb on + strand, within BBR_RS14320 at 989.881 kb on + strand at 989.941 kb on + strand at 989.941 kb on + strand at 989.941 kb on + strand at 989.949 kb on - strand at 989.954 kb on + strand at 989.954 kb on + strand at 989.963 kb on - strand at 989.978 kb on - strand at 989.980 kb on - strand at 990.008 kb on - strand at 990.084 kb on + strand, within BBR_RS14325 at 990.114 kb on + strand, within BBR_RS14325 at 990.114 kb on + strand, within BBR_RS14325 at 990.165 kb on - strand, within BBR_RS14325 at 990.204 kb on + strand, within BBR_RS14325 at 990.260 kb on + strand, within BBR_RS14325 at 990.260 kb on + strand, within BBR_RS14325 at 990.269 kb on - strand, within BBR_RS14325 at 990.275 kb on - strand, within BBR_RS14325 at 990.301 kb on - strand, within BBR_RS14325 at 990.309 kb on + strand, within BBR_RS14325 at 990.372 kb on + strand, within BBR_RS14325 at 990.411 kb on - strand, within BBR_RS14325 at 990.444 kb on + strand, within BBR_RS14325 at 990.475 kb on + strand, within BBR_RS14325 at 990.475 kb on + strand, within BBR_RS14325 at 990.489 kb on + strand, within BBR_RS14325 at 990.561 kb on - strand, within BBR_RS14325 at 990.943 kb on - strand at 990.960 kb on + strand at 991.420 kb on - strand, within BBR_RS14330 at 991.487 kb on + strand, within BBR_RS14330
Per-strain Table
Position Strand Gene LocusTag Fraction Recovery broth with Chenodeoxycholic 2.5 mM remove 989,034 - -1.7 989,035 + +0.7 989,043 - -0.2 989,080 - +1.4 989,115 - -1.5 989,183 + BBR_RS14320 0.15 -1.9 989,205 - BBR_RS14320 0.17 -1.1 989,235 + BBR_RS14320 0.21 +0.7 989,307 + BBR_RS14320 0.28 -1.6 989,315 - BBR_RS14320 0.29 +0.3 989,322 + BBR_RS14320 0.30 +0.2 989,370 + BBR_RS14320 0.35 +1.3 989,375 + BBR_RS14320 0.36 +0.5 989,378 - BBR_RS14320 0.36 -1.0 989,380 + BBR_RS14320 0.36 -0.5 989,400 + BBR_RS14320 0.39 -4.1 989,426 - BBR_RS14320 0.41 +0.8 989,455 + BBR_RS14320 0.45 -2.0 989,539 + BBR_RS14320 0.54 -3.0 989,551 - BBR_RS14320 0.55 +0.4 989,601 + BBR_RS14320 0.60 +0.4 989,645 - BBR_RS14320 0.65 -0.6 989,666 + BBR_RS14320 0.67 +0.3 989,689 + BBR_RS14320 0.70 -0.1 989,745 + BBR_RS14320 0.76 +0.7 989,753 - BBR_RS14320 0.77 +1.0 989,757 + BBR_RS14320 0.77 -1.7 989,759 - BBR_RS14320 0.78 -0.7 989,791 - BBR_RS14320 0.81 +0.7 989,811 - BBR_RS14320 0.83 +0.4 989,850 + BBR_RS14320 0.87 +1.1 989,850 + BBR_RS14320 0.87 -0.1 989,881 + -0.4 989,941 + +1.7 989,941 + -0.2 989,941 + -2.5 989,949 - +0.6 989,954 + -0.6 989,954 + -0.4 989,963 - +0.9 989,978 - -0.0 989,980 - -1.7 990,008 - +1.4 990,084 + BBR_RS14325 0.11 +1.5 990,114 + BBR_RS14325 0.16 +0.7 990,114 + BBR_RS14325 0.16 +1.9 990,165 - BBR_RS14325 0.23 -0.0 990,204 + BBR_RS14325 0.29 +0.8 990,260 + BBR_RS14325 0.37 +0.1 990,260 + BBR_RS14325 0.37 +2.6 990,269 - BBR_RS14325 0.39 +1.0 990,275 - BBR_RS14325 0.40 -0.7 990,301 - BBR_RS14325 0.43 -1.9 990,309 + BBR_RS14325 0.45 +2.2 990,372 + BBR_RS14325 0.54 +2.7 990,411 - BBR_RS14325 0.60 -0.4 990,444 + BBR_RS14325 0.65 +1.2 990,475 + BBR_RS14325 0.69 +0.8 990,475 + BBR_RS14325 0.69 +0.3 990,489 + BBR_RS14325 0.71 -1.2 990,561 - BBR_RS14325 0.82 -1.0 990,943 - +0.3 990,960 + +0.6 991,420 - BBR_RS14330 0.31 +2.6 991,487 + BBR_RS14330 0.35 +0.6
Or see this region's nucleotide sequence