Experiment: Cobalt chloride 0.05 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BT1923 and BT1924 are separated by 213 nucleotides BT1924 and BT1925 are separated by 279 nucleotides BT1925 and BT1926 are separated by 229 nucleotides
BT1923: BT1923 - O-acetylhomoserine (thiol)-lyase (NCBI ptt file), at 2,412,202 to 2,413,482
BT1923
BT1924: BT1924 - putative metal-dependent hydrolase (NCBI ptt file), at 2,413,696 to 2,414,463
BT1924
BT1925: BT1925 - hypothetical protein (NCBI ptt file), at 2,414,743 to 2,415,138
BT1925
BT1926: BT1926 - hypothetical protein (NCBI ptt file), at 2,415,368 to 2,416,234
BT1926
Position (kb)
2413
2414
2415 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2412.753 kb on + strand, within BT1923 at 2412.768 kb on + strand, within BT1923 at 2412.768 kb on + strand, within BT1923 at 2412.769 kb on - strand, within BT1923 at 2412.769 kb on - strand, within BT1923 at 2412.827 kb on - strand, within BT1923 at 2412.852 kb on - strand, within BT1923 at 2412.937 kb on + strand, within BT1923 at 2412.937 kb on + strand, within BT1923 at 2412.952 kb on + strand, within BT1923 at 2412.979 kb on + strand, within BT1923 at 2412.979 kb on + strand, within BT1923 at 2412.979 kb on + strand, within BT1923 at 2412.979 kb on + strand, within BT1923 at 2412.980 kb on - strand, within BT1923 at 2412.980 kb on - strand, within BT1923 at 2413.123 kb on + strand, within BT1923 at 2413.132 kb on - strand, within BT1923 at 2413.159 kb on + strand, within BT1923 at 2413.183 kb on + strand, within BT1923 at 2413.183 kb on + strand, within BT1923 at 2413.184 kb on - strand, within BT1923 at 2413.276 kb on - strand, within BT1923 at 2413.286 kb on + strand, within BT1923 at 2413.326 kb on + strand, within BT1923 at 2413.397 kb on + strand at 2413.410 kb on - strand at 2413.425 kb on - strand at 2413.491 kb on + strand at 2413.494 kb on + strand at 2413.495 kb on - strand at 2413.585 kb on - strand at 2413.585 kb on - strand at 2413.593 kb on - strand at 2413.627 kb on - strand at 2413.646 kb on + strand at 2413.690 kb on - strand at 2413.692 kb on - strand at 2413.710 kb on - strand at 2413.716 kb on - strand at 2413.777 kb on - strand, within BT1924 at 2413.877 kb on + strand, within BT1924 at 2413.883 kb on - strand, within BT1924 at 2413.883 kb on - strand, within BT1924 at 2413.956 kb on + strand, within BT1924 at 2414.097 kb on - strand, within BT1924 at 2414.132 kb on + strand, within BT1924 at 2414.140 kb on + strand, within BT1924 at 2414.141 kb on - strand, within BT1924 at 2414.141 kb on - strand, within BT1924 at 2414.141 kb on - strand, within BT1924 at 2414.142 kb on + strand, within BT1924 at 2414.145 kb on + strand, within BT1924 at 2414.164 kb on - strand, within BT1924 at 2414.196 kb on + strand at 2414.196 kb on + strand, within BT1924 at 2414.197 kb on - strand, within BT1924 at 2414.247 kb on + strand, within BT1924 at 2414.281 kb on - strand, within BT1924 at 2414.308 kb on + strand, within BT1924 at 2414.311 kb on - strand, within BT1924 at 2414.331 kb on + strand, within BT1924 at 2414.331 kb on + strand, within BT1924 at 2414.333 kb on + strand, within BT1924 at 2414.333 kb on + strand, within BT1924 at 2414.380 kb on + strand, within BT1924 at 2414.436 kb on - strand at 2414.441 kb on + strand at 2414.442 kb on - strand at 2414.442 kb on - strand at 2414.442 kb on - strand at 2414.445 kb on + strand at 2414.462 kb on - strand at 2414.467 kb on + strand at 2414.475 kb on + strand at 2414.707 kb on + strand at 2414.773 kb on - strand at 2414.786 kb on - strand, within BT1925 at 2414.796 kb on + strand, within BT1925 at 2414.810 kb on + strand, within BT1925 at 2414.818 kb on + strand, within BT1925 at 2414.887 kb on + strand, within BT1925 at 2414.887 kb on + strand, within BT1925 at 2414.889 kb on + strand, within BT1925 at 2414.965 kb on + strand, within BT1925 at 2414.966 kb on - strand, within BT1925 at 2415.040 kb on + strand at 2415.221 kb on + strand at 2415.221 kb on + strand at 2415.222 kb on - strand at 2415.224 kb on + strand at 2415.255 kb on - strand at 2415.256 kb on + strand at 2415.257 kb on - strand at 2415.263 kb on - strand at 2415.353 kb on + strand at 2415.353 kb on + strand at 2415.353 kb on + strand at 2415.434 kb on - strand at 2415.435 kb on + strand at 2415.439 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Cobalt chloride 0.05 mM remove 2,412,753 + BT1923 0.43 +0.4 2,412,768 + BT1923 0.44 -2.4 2,412,768 + BT1923 0.44 -0.0 2,412,769 - BT1923 0.44 -0.8 2,412,769 - BT1923 0.44 -1.0 2,412,827 - BT1923 0.49 -0.8 2,412,852 - BT1923 0.51 -0.2 2,412,937 + BT1923 0.57 -0.6 2,412,937 + BT1923 0.57 +0.2 2,412,952 + BT1923 0.59 -2.1 2,412,979 + BT1923 0.61 -2.1 2,412,979 + BT1923 0.61 +1.6 2,412,979 + BT1923 0.61 -0.8 2,412,979 + BT1923 0.61 -0.5 2,412,980 - BT1923 0.61 +0.6 2,412,980 - BT1923 0.61 -0.6 2,413,123 + BT1923 0.72 -0.7 2,413,132 - BT1923 0.73 -0.4 2,413,159 + BT1923 0.75 -1.3 2,413,183 + BT1923 0.77 -0.6 2,413,183 + BT1923 0.77 -0.7 2,413,184 - BT1923 0.77 -0.8 2,413,276 - BT1923 0.84 -1.5 2,413,286 + BT1923 0.85 -0.0 2,413,326 + BT1923 0.88 -1.0 2,413,397 + -1.5 2,413,410 - -0.9 2,413,425 - -0.4 2,413,491 + -0.8 2,413,494 + +0.2 2,413,495 - +1.6 2,413,585 - -0.4 2,413,585 - -1.8 2,413,593 - +0.1 2,413,627 - -0.9 2,413,646 + +0.6 2,413,690 - +0.6 2,413,692 - -0.0 2,413,710 - +1.8 2,413,716 - -0.8 2,413,777 - BT1924 0.11 +1.0 2,413,877 + BT1924 0.24 +0.9 2,413,883 - BT1924 0.24 +1.1 2,413,883 - BT1924 0.24 -0.6 2,413,956 + BT1924 0.34 -0.3 2,414,097 - BT1924 0.52 -0.7 2,414,132 + BT1924 0.57 -0.4 2,414,140 + BT1924 0.58 -0.3 2,414,141 - BT1924 0.58 -0.8 2,414,141 - BT1924 0.58 -1.4 2,414,141 - BT1924 0.58 -0.0 2,414,142 + BT1924 0.58 -0.3 2,414,145 + BT1924 0.58 +0.6 2,414,164 - BT1924 0.61 -1.0 2,414,196 + +0.6 2,414,196 + BT1924 0.65 -1.9 2,414,197 - BT1924 0.65 -1.3 2,414,247 + BT1924 0.72 -0.2 2,414,281 - BT1924 0.76 -0.0 2,414,308 + BT1924 0.80 +0.1 2,414,311 - BT1924 0.80 -1.1 2,414,331 + BT1924 0.83 -0.1 2,414,331 + BT1924 0.83 +1.1 2,414,333 + BT1924 0.83 -0.1 2,414,333 + BT1924 0.83 +2.6 2,414,380 + BT1924 0.89 +0.1 2,414,436 - -2.0 2,414,441 + -0.9 2,414,442 - +0.7 2,414,442 - +0.1 2,414,442 - -0.6 2,414,445 + -1.4 2,414,462 - +1.4 2,414,467 + +0.3 2,414,475 + +1.0 2,414,707 + -0.8 2,414,773 - +1.1 2,414,786 - BT1925 0.11 -1.0 2,414,796 + BT1925 0.13 -1.0 2,414,810 + BT1925 0.17 -0.2 2,414,818 + BT1925 0.19 -1.6 2,414,887 + BT1925 0.36 -0.9 2,414,887 + BT1925 0.36 -0.7 2,414,889 + BT1925 0.37 -1.0 2,414,965 + BT1925 0.56 -1.4 2,414,966 - BT1925 0.56 -0.5 2,415,040 + +0.6 2,415,221 + -1.3 2,415,221 + -1.0 2,415,222 - -0.5 2,415,224 + -0.0 2,415,255 - -1.0 2,415,256 + -0.4 2,415,257 - +0.6 2,415,263 - -1.0 2,415,353 + -1.4 2,415,353 + -1.0 2,415,353 + +1.6 2,415,434 - -1.6 2,415,435 + -1.1 2,415,439 + -0.7
Or see this region's nucleotide sequence