Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_652 and Pf1N1B4_653 are separated by 2 nucleotides Pf1N1B4_653 and Pf1N1B4_654 are separated by 134 nucleotides Pf1N1B4_654 and Pf1N1B4_655 overlap by 4 nucleotides Pf1N1B4_655 and Pf1N1B4_656 are separated by 164 nucleotides
Pf1N1B4_652 - Sensory box histidine kinase, at 745,642 to 747,678
_652
Pf1N1B4_653 - ATP:Cob(I)alamin adenosyltransferase (EC 2.5.1.17), at 747,681 to 748,262
_653
Pf1N1B4_654 - Mutator mutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-) / Thiamin-phosphate pyrophosphorylase-like protein, at 748,397 to 749,341
_654
Pf1N1B4_655 - Glutathione S-transferase (EC 2.5.1.18), at 749,338 to 749,970
_655
Pf1N1B4_656 - Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1), at 750,135 to 751,352
_656
Position (kb)
748
749
750 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 747.474 kb on + strand, within Pf1N1B4_652 at 747.474 kb on + strand, within Pf1N1B4_652 at 747.474 kb on + strand, within Pf1N1B4_652 at 747.474 kb on + strand, within Pf1N1B4_652 at 747.474 kb on + strand, within Pf1N1B4_652 at 747.474 kb on + strand, within Pf1N1B4_652 at 747.475 kb on - strand at 747.475 kb on - strand at 747.541 kb on + strand at 747.541 kb on + strand at 747.676 kb on + strand at 747.705 kb on + strand at 747.706 kb on - strand at 747.706 kb on - strand at 747.854 kb on + strand, within Pf1N1B4_653 at 747.854 kb on + strand, within Pf1N1B4_653 at 747.854 kb on + strand, within Pf1N1B4_653 at 747.854 kb on + strand, within Pf1N1B4_653 at 747.854 kb on + strand, within Pf1N1B4_653 at 747.854 kb on + strand, within Pf1N1B4_653 at 747.854 kb on + strand, within Pf1N1B4_653 at 747.854 kb on + strand, within Pf1N1B4_653 at 747.854 kb on + strand, within Pf1N1B4_653 at 747.855 kb on - strand, within Pf1N1B4_653 at 747.855 kb on - strand, within Pf1N1B4_653 at 747.855 kb on - strand, within Pf1N1B4_653 at 747.855 kb on - strand, within Pf1N1B4_653 at 747.858 kb on + strand, within Pf1N1B4_653 at 747.858 kb on + strand, within Pf1N1B4_653 at 747.858 kb on + strand, within Pf1N1B4_653 at 747.858 kb on + strand, within Pf1N1B4_653 at 747.945 kb on + strand, within Pf1N1B4_653 at 747.946 kb on - strand, within Pf1N1B4_653 at 747.946 kb on - strand, within Pf1N1B4_653 at 748.072 kb on + strand, within Pf1N1B4_653 at 748.072 kb on + strand, within Pf1N1B4_653 at 748.072 kb on + strand, within Pf1N1B4_653 at 748.072 kb on + strand, within Pf1N1B4_653 at 748.155 kb on + strand, within Pf1N1B4_653 at 748.155 kb on + strand, within Pf1N1B4_653 at 748.155 kb on + strand, within Pf1N1B4_653 at 748.155 kb on + strand, within Pf1N1B4_653 at 748.155 kb on + strand, within Pf1N1B4_653 at 748.155 kb on + strand, within Pf1N1B4_653 at 748.155 kb on + strand, within Pf1N1B4_653 at 748.156 kb on - strand, within Pf1N1B4_653 at 748.156 kb on - strand, within Pf1N1B4_653 at 748.156 kb on - strand, within Pf1N1B4_653 at 748.156 kb on - strand, within Pf1N1B4_653 at 748.157 kb on + strand, within Pf1N1B4_653 at 748.157 kb on + strand, within Pf1N1B4_653 at 748.157 kb on + strand, within Pf1N1B4_653 at 748.158 kb on - strand, within Pf1N1B4_653 at 748.158 kb on - strand, within Pf1N1B4_653 at 748.260 kb on + strand at 748.320 kb on + strand at 748.508 kb on - strand, within Pf1N1B4_654 at 748.525 kb on + strand, within Pf1N1B4_654 at 748.525 kb on + strand, within Pf1N1B4_654 at 748.525 kb on + strand, within Pf1N1B4_654 at 748.526 kb on - strand, within Pf1N1B4_654 at 748.526 kb on - strand, within Pf1N1B4_654 at 748.526 kb on - strand, within Pf1N1B4_654 at 748.526 kb on - strand, within Pf1N1B4_654 at 748.698 kb on + strand, within Pf1N1B4_654 at 748.698 kb on + strand, within Pf1N1B4_654 at 748.698 kb on + strand, within Pf1N1B4_654 at 748.698 kb on + strand, within Pf1N1B4_654 at 748.698 kb on + strand, within Pf1N1B4_654 at 748.698 kb on + strand, within Pf1N1B4_654 at 748.698 kb on + strand, within Pf1N1B4_654 at 748.699 kb on - strand, within Pf1N1B4_654 at 748.699 kb on - strand, within Pf1N1B4_654 at 748.699 kb on - strand, within Pf1N1B4_654 at 748.939 kb on + strand, within Pf1N1B4_654 at 748.947 kb on + strand, within Pf1N1B4_654 at 748.947 kb on + strand, within Pf1N1B4_654 at 748.948 kb on - strand, within Pf1N1B4_654 at 749.005 kb on - strand, within Pf1N1B4_654 at 749.324 kb on + strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.325 kb on - strand at 749.347 kb on + strand at 749.347 kb on + strand at 749.348 kb on - strand at 749.348 kb on - strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.396 kb on + strand at 749.397 kb on - strand at 749.411 kb on + strand, within Pf1N1B4_655 at 749.411 kb on + strand, within Pf1N1B4_655 at 749.411 kb on + strand, within Pf1N1B4_655 at 749.411 kb on + strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.412 kb on - strand, within Pf1N1B4_655 at 749.437 kb on + strand, within Pf1N1B4_655 at 749.437 kb on + strand, within Pf1N1B4_655 at 749.444 kb on + strand, within Pf1N1B4_655 at 749.444 kb on + strand, within Pf1N1B4_655 at 749.444 kb on + strand, within Pf1N1B4_655 at 749.444 kb on + strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.445 kb on - strand, within Pf1N1B4_655 at 749.459 kb on - strand, within Pf1N1B4_655 at 749.634 kb on + strand, within Pf1N1B4_655 at 749.634 kb on + strand, within Pf1N1B4_655 at 749.634 kb on + strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.635 kb on - strand, within Pf1N1B4_655 at 749.750 kb on + strand, within Pf1N1B4_655 at 749.751 kb on - strand, within Pf1N1B4_655 at 749.882 kb on + strand, within Pf1N1B4_655 at 749.989 kb on - strand at 750.136 kb on + strand at 750.136 kb on + strand at 750.136 kb on + strand at 750.136 kb on + strand at 750.136 kb on + strand at 750.137 kb on - strand at 750.137 kb on - strand at 750.137 kb on - strand at 750.137 kb on - strand at 750.137 kb on - strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.140 kb on + strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.141 kb on - strand at 750.145 kb on + strand at 750.166 kb on + strand at 750.166 kb on + strand at 750.167 kb on - strand at 750.167 kb on - strand at 750.167 kb on - strand at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.280 kb on + strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.281 kb on - strand, within Pf1N1B4_656 at 750.330 kb on + strand, within Pf1N1B4_656 at 750.330 kb on + strand at 750.330 kb on + strand, within Pf1N1B4_656 at 750.330 kb on + strand, within Pf1N1B4_656 at 750.330 kb on + strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656 at 750.331 kb on - strand, within Pf1N1B4_656
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 747,474 + Pf1N1B4_652 0.90 +1.6 747,474 + Pf1N1B4_652 0.90 +2.6 747,474 + Pf1N1B4_652 0.90 +1.4 747,474 + Pf1N1B4_652 0.90 +1.1 747,474 + Pf1N1B4_652 0.90 +1.0 747,474 + Pf1N1B4_652 0.90 +1.9 747,475 - +2.1 747,475 - +1.0 747,541 + +2.5 747,541 + +4.0 747,676 + +1.3 747,705 + +0.3 747,706 - -1.8 747,706 - +0.6 747,854 + Pf1N1B4_653 0.30 -0.8 747,854 + Pf1N1B4_653 0.30 -0.5 747,854 + Pf1N1B4_653 0.30 +0.7 747,854 + Pf1N1B4_653 0.30 +0.8 747,854 + Pf1N1B4_653 0.30 +1.5 747,854 + Pf1N1B4_653 0.30 +0.3 747,854 + Pf1N1B4_653 0.30 +1.1 747,854 + Pf1N1B4_653 0.30 +1.2 747,854 + Pf1N1B4_653 0.30 +1.8 747,855 - Pf1N1B4_653 0.30 +2.1 747,855 - Pf1N1B4_653 0.30 -0.5 747,855 - Pf1N1B4_653 0.30 +0.9 747,855 - Pf1N1B4_653 0.30 +1.7 747,858 + Pf1N1B4_653 0.30 +2.1 747,858 + Pf1N1B4_653 0.30 +3.0 747,858 + Pf1N1B4_653 0.30 +0.8 747,858 + Pf1N1B4_653 0.30 -0.7 747,945 + Pf1N1B4_653 0.45 +0.8 747,946 - Pf1N1B4_653 0.46 +0.1 747,946 - Pf1N1B4_653 0.46 +0.2 748,072 + Pf1N1B4_653 0.67 -3.0 748,072 + Pf1N1B4_653 0.67 -0.8 748,072 + Pf1N1B4_653 0.67 +0.3 748,072 + Pf1N1B4_653 0.67 +0.8 748,155 + Pf1N1B4_653 0.81 +0.1 748,155 + Pf1N1B4_653 0.81 +1.2 748,155 + Pf1N1B4_653 0.81 +1.4 748,155 + Pf1N1B4_653 0.81 +1.1 748,155 + Pf1N1B4_653 0.81 -2.3 748,155 + Pf1N1B4_653 0.81 +1.4 748,155 + Pf1N1B4_653 0.81 +1.4 748,156 - Pf1N1B4_653 0.82 -3.6 748,156 - Pf1N1B4_653 0.82 -0.0 748,156 - Pf1N1B4_653 0.82 +0.1 748,156 - Pf1N1B4_653 0.82 -0.6 748,157 + Pf1N1B4_653 0.82 +0.5 748,157 + Pf1N1B4_653 0.82 -1.0 748,157 + Pf1N1B4_653 0.82 +0.9 748,158 - Pf1N1B4_653 0.82 -0.7 748,158 - Pf1N1B4_653 0.82 -2.0 748,260 + -0.9 748,320 + +1.0 748,508 - Pf1N1B4_654 0.12 -2.5 748,525 + Pf1N1B4_654 0.14 -1.2 748,525 + Pf1N1B4_654 0.14 -1.6 748,525 + Pf1N1B4_654 0.14 +0.3 748,526 - Pf1N1B4_654 0.14 -2.0 748,526 - Pf1N1B4_654 0.14 -1.5 748,526 - Pf1N1B4_654 0.14 -1.9 748,526 - Pf1N1B4_654 0.14 -1.5 748,698 + Pf1N1B4_654 0.32 +0.1 748,698 + Pf1N1B4_654 0.32 -1.1 748,698 + Pf1N1B4_654 0.32 -2.9 748,698 + Pf1N1B4_654 0.32 -0.9 748,698 + Pf1N1B4_654 0.32 -2.9 748,698 + Pf1N1B4_654 0.32 -2.3 748,698 + Pf1N1B4_654 0.32 -1.1 748,699 - Pf1N1B4_654 0.32 -1.0 748,699 - Pf1N1B4_654 0.32 -1.4 748,699 - Pf1N1B4_654 0.32 -1.5 748,939 + Pf1N1B4_654 0.57 -1.8 748,947 + Pf1N1B4_654 0.58 -0.6 748,947 + Pf1N1B4_654 0.58 -1.8 748,948 - Pf1N1B4_654 0.58 +0.2 749,005 - Pf1N1B4_654 0.64 -0.9 749,324 + -1.1 749,325 - +0.2 749,325 - -1.7 749,325 - +2.9 749,325 - +0.4 749,325 - +0.7 749,325 - +0.6 749,347 + +1.5 749,347 + -0.5 749,348 - -1.2 749,348 - +1.0 749,396 + +3.4 749,396 + +0.1 749,396 + +0.3 749,396 + +1.4 749,396 + +0.4 749,396 + +1.3 749,397 - +2.1 749,411 + Pf1N1B4_655 0.12 +0.8 749,411 + Pf1N1B4_655 0.12 -3.0 749,411 + Pf1N1B4_655 0.12 +1.5 749,411 + Pf1N1B4_655 0.12 -1.3 749,412 - Pf1N1B4_655 0.12 -0.4 749,412 - Pf1N1B4_655 0.12 -0.8 749,412 - Pf1N1B4_655 0.12 +0.6 749,412 - Pf1N1B4_655 0.12 +1.3 749,412 - Pf1N1B4_655 0.12 -1.3 749,437 + Pf1N1B4_655 0.16 +2.3 749,437 + Pf1N1B4_655 0.16 -0.5 749,444 + Pf1N1B4_655 0.17 +2.3 749,444 + Pf1N1B4_655 0.17 +1.0 749,444 + Pf1N1B4_655 0.17 -0.2 749,444 + Pf1N1B4_655 0.17 +1.6 749,445 - Pf1N1B4_655 0.17 +0.8 749,445 - Pf1N1B4_655 0.17 +0.6 749,445 - Pf1N1B4_655 0.17 +0.7 749,445 - Pf1N1B4_655 0.17 -1.0 749,445 - Pf1N1B4_655 0.17 +1.3 749,459 - Pf1N1B4_655 0.19 -1.3 749,634 + Pf1N1B4_655 0.47 +1.0 749,634 + Pf1N1B4_655 0.47 +1.3 749,634 + Pf1N1B4_655 0.47 -1.8 749,635 - Pf1N1B4_655 0.47 +0.7 749,635 - Pf1N1B4_655 0.47 -0.6 749,635 - Pf1N1B4_655 0.47 -0.7 749,635 - Pf1N1B4_655 0.47 +0.1 749,635 - Pf1N1B4_655 0.47 -2.0 749,750 + Pf1N1B4_655 0.65 +1.6 749,751 - Pf1N1B4_655 0.65 +1.6 749,882 + Pf1N1B4_655 0.86 +1.1 749,989 - +0.3 750,136 + -0.7 750,136 + +0.6 750,136 + +0.7 750,136 + -1.0 750,136 + -0.7 750,137 - +0.3 750,137 - -1.2 750,137 - +0.1 750,137 - -0.7 750,137 - -1.3 750,140 + +2.2 750,140 + +2.8 750,140 + +1.2 750,140 + +1.4 750,140 + +2.1 750,140 + +2.4 750,140 + +1.8 750,140 + +3.9 750,140 + +4.0 750,140 + +2.4 750,140 + +3.3 750,140 + -0.7 750,140 + +2.9 750,140 + +1.9 750,140 + +2.6 750,141 - +2.1 750,141 - +2.3 750,141 - +1.9 750,141 - +2.3 750,141 - -0.1 750,141 - +2.9 750,141 - +3.3 750,141 - -0.4 750,141 - +1.9 750,141 - +2.2 750,141 - +1.0 750,141 - +1.5 750,141 - +2.7 750,141 - +2.7 750,141 - +1.8 750,145 + +2.2 750,166 + +2.2 750,166 + +2.4 750,167 - +2.5 750,167 - +2.6 750,167 - +2.7 750,280 + Pf1N1B4_656 0.12 +2.0 750,280 + Pf1N1B4_656 0.12 +2.9 750,280 + Pf1N1B4_656 0.12 +3.0 750,280 + Pf1N1B4_656 0.12 +2.9 750,280 + Pf1N1B4_656 0.12 +2.9 750,280 + Pf1N1B4_656 0.12 +2.6 750,281 - Pf1N1B4_656 0.12 +3.2 750,281 - Pf1N1B4_656 0.12 +0.2 750,281 - Pf1N1B4_656 0.12 +2.7 750,281 - Pf1N1B4_656 0.12 +1.5 750,281 - Pf1N1B4_656 0.12 +2.4 750,281 - Pf1N1B4_656 0.12 +2.0 750,281 - Pf1N1B4_656 0.12 +1.8 750,330 + Pf1N1B4_656 0.16 +3.3 750,330 + +2.9 750,330 + Pf1N1B4_656 0.16 +2.6 750,330 + Pf1N1B4_656 0.16 +2.3 750,330 + Pf1N1B4_656 0.16 +2.7 750,331 - Pf1N1B4_656 0.16 -1.8 750,331 - Pf1N1B4_656 0.16 +3.3 750,331 - Pf1N1B4_656 0.16 +2.8 750,331 - Pf1N1B4_656 0.16 +3.1 750,331 - Pf1N1B4_656 0.16 +2.7
Or see this region's nucleotide sequence