Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_619 and Pf1N1B4_620 are separated by 457 nucleotides Pf1N1B4_620 and Pf1N1B4_621 are separated by 11 nucleotides
Pf1N1B4_619 - Sigma-54 dependent transcriptional regulator/sensory box protein, at 715,265 to 716,773
_619
Pf1N1B4_620 - Glycine cleavage system H protein, at 717,231 to 717,614
_620
Pf1N1B4_621 - Glycine dehydrogenase [decarboxylating] (glycine cleavage system P protein) (EC 1.4.4.2), at 717,626 to 720,478
_621
Position (kb) 717
718 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 716.396 kb on + strand, within Pf1N1B4_619 at 716.397 kb on - strand, within Pf1N1B4_619 at 716.460 kb on - strand at 716.472 kb on + strand, within Pf1N1B4_619 at 716.670 kb on - strand at 716.746 kb on + strand at 716.746 kb on + strand at 716.746 kb on + strand at 716.747 kb on - strand at 716.747 kb on - strand at 716.747 kb on - strand at 716.750 kb on + strand at 716.751 kb on - strand at 716.757 kb on + strand at 716.757 kb on + strand at 716.757 kb on + strand at 716.758 kb on - strand at 716.758 kb on - strand at 716.758 kb on - strand at 716.761 kb on + strand at 716.761 kb on + strand at 716.761 kb on + strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.762 kb on - strand at 716.843 kb on - strand at 716.846 kb on + strand at 716.846 kb on + strand at 716.846 kb on + strand at 716.847 kb on - strand at 716.858 kb on + strand at 716.859 kb on - strand at 716.871 kb on + strand at 716.871 kb on + strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 716.872 kb on - strand at 717.072 kb on - strand at 717.221 kb on - strand at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.426 kb on + strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand, within Pf1N1B4_620 at 717.427 kb on - strand at 717.580 kb on - strand at 717.637 kb on + strand at 717.637 kb on + strand at 717.638 kb on - strand at 717.869 kb on + strand at 717.869 kb on + strand at 717.869 kb on + strand at 717.870 kb on - strand at 717.870 kb on - strand at 717.870 kb on - strand at 717.885 kb on - strand at 717.898 kb on + strand at 717.899 kb on - strand at 717.899 kb on - strand at 717.899 kb on - strand at 717.899 kb on - strand at 717.954 kb on - strand, within Pf1N1B4_621 at 717.954 kb on - strand, within Pf1N1B4_621 at 717.963 kb on - strand, within Pf1N1B4_621 at 718.475 kb on + strand at 718.475 kb on + strand, within Pf1N1B4_621 at 718.592 kb on - strand, within Pf1N1B4_621
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 716,396 + Pf1N1B4_619 0.75 -1.5 716,397 - Pf1N1B4_619 0.75 -1.2 716,460 - +1.1 716,472 + Pf1N1B4_619 0.80 -1.6 716,670 - -2.5 716,746 + -0.3 716,746 + -2.0 716,746 + -1.6 716,747 - -1.6 716,747 - -2.8 716,747 - -1.6 716,750 + +0.4 716,751 - -2.9 716,757 + +0.0 716,757 + -1.9 716,757 + -0.7 716,758 - -2.6 716,758 - -1.8 716,758 - +0.1 716,761 + +1.5 716,761 + +0.7 716,761 + -1.3 716,762 - -3.8 716,762 - -3.3 716,762 - -0.2 716,762 - +0.5 716,762 - -2.7 716,762 - -2.2 716,843 - +1.1 716,846 + -2.0 716,846 + -3.0 716,846 + -0.7 716,847 - +0.0 716,858 + +0.1 716,859 - -0.3 716,871 + +0.2 716,871 + -0.6 716,872 - -0.3 716,872 - +1.4 716,872 - +0.4 716,872 - +0.5 716,872 - +0.1 717,072 - -2.2 717,221 - -2.0 717,426 + Pf1N1B4_620 0.51 -0.9 717,426 + Pf1N1B4_620 0.51 -0.6 717,426 + Pf1N1B4_620 0.51 -2.8 717,426 + Pf1N1B4_620 0.51 -1.3 717,427 - Pf1N1B4_620 0.51 -2.4 717,427 - Pf1N1B4_620 0.51 -2.2 717,427 - Pf1N1B4_620 0.51 -1.5 717,427 - -1.2 717,427 - Pf1N1B4_620 0.51 -2.8 717,427 - Pf1N1B4_620 0.51 -2.0 717,427 - Pf1N1B4_620 0.51 -0.8 717,427 - -0.5 717,580 - -2.0 717,637 + -1.9 717,637 + -1.8 717,638 - -2.8 717,869 + -2.4 717,869 + -2.0 717,869 + +0.5 717,870 - +1.4 717,870 - -0.4 717,870 - -2.4 717,885 - -3.0 717,898 + -1.7 717,899 - -2.3 717,899 - -3.1 717,899 - -2.6 717,899 - -3.0 717,954 - Pf1N1B4_621 0.11 -2.8 717,954 - Pf1N1B4_621 0.11 +3.1 717,963 - Pf1N1B4_621 0.12 +0.7 718,475 + -0.9 718,475 + Pf1N1B4_621 0.30 -1.3 718,592 - Pf1N1B4_621 0.34 -2.6
Or see this region's nucleotide sequence