Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5964 and Pf1N1B4_5965 are separated by 11 nucleotides Pf1N1B4_5965 and Pf1N1B4_5966 overlap by 8 nucleotides Pf1N1B4_5966 and Pf1N1B4_5967 overlap by 4 nucleotides
Pf1N1B4_5964 - 4-hydroxyproline epimerase (EC 5.1.1.8), at 412,354 to 413,286
_5964
Pf1N1B4_5965 - D-amino-acid oxidase (EC 1.4.3.3), at 413,298 to 414,398
_5965
Pf1N1B4_5966 - Hypothetical, similar to sarcosine oxidase alpha subunit, 2Fe-2S domain, at 414,391 to 414,627
_5966
Pf1N1B4_5967 - Putative oxidoreductase in 4-hydroxyproline catabolic gene cluster, at 414,624 to 415,880
_5967
Position (kb) 413
414
415 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 412.907 kb on - strand, within Pf1N1B4_5964 at 412.907 kb on - strand, within Pf1N1B4_5964 at 412.907 kb on - strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.908 kb on + strand, within Pf1N1B4_5964 at 412.909 kb on - strand at 412.909 kb on - strand, within Pf1N1B4_5964 at 412.912 kb on + strand, within Pf1N1B4_5964 at 412.913 kb on - strand, within Pf1N1B4_5964 at 412.913 kb on - strand, within Pf1N1B4_5964 at 412.913 kb on - strand, within Pf1N1B4_5964 at 412.913 kb on - strand, within Pf1N1B4_5964 at 412.944 kb on + strand, within Pf1N1B4_5964 at 412.944 kb on + strand, within Pf1N1B4_5964 at 412.946 kb on + strand at 412.946 kb on + strand, within Pf1N1B4_5964 at 412.946 kb on + strand, within Pf1N1B4_5964 at 412.947 kb on - strand, within Pf1N1B4_5964 at 412.947 kb on - strand, within Pf1N1B4_5964 at 412.947 kb on - strand, within Pf1N1B4_5964 at 413.170 kb on + strand, within Pf1N1B4_5964 at 413.171 kb on - strand, within Pf1N1B4_5964 at 413.171 kb on - strand, within Pf1N1B4_5964 at 413.221 kb on - strand at 413.595 kb on + strand, within Pf1N1B4_5965 at 413.595 kb on + strand, within Pf1N1B4_5965 at 413.595 kb on + strand, within Pf1N1B4_5965 at 413.595 kb on + strand, within Pf1N1B4_5965 at 413.894 kb on - strand, within Pf1N1B4_5965 at 413.958 kb on - strand, within Pf1N1B4_5965 at 413.976 kb on + strand, within Pf1N1B4_5965 at 413.977 kb on - strand at 414.023 kb on + strand, within Pf1N1B4_5965 at 414.138 kb on + strand, within Pf1N1B4_5965 at 414.138 kb on + strand, within Pf1N1B4_5965 at 414.139 kb on - strand, within Pf1N1B4_5965 at 414.391 kb on - strand at 414.918 kb on + strand, within Pf1N1B4_5967 at 414.919 kb on - strand, within Pf1N1B4_5967 at 414.955 kb on + strand, within Pf1N1B4_5967 at 414.956 kb on - strand, within Pf1N1B4_5967 at 414.956 kb on - strand, within Pf1N1B4_5967 at 414.956 kb on - strand, within Pf1N1B4_5967
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 412,907 - Pf1N1B4_5964 0.59 -1.5 412,907 - Pf1N1B4_5964 0.59 +2.4 412,907 - Pf1N1B4_5964 0.59 -0.6 412,908 + Pf1N1B4_5964 0.59 -0.1 412,908 + Pf1N1B4_5964 0.59 -0.2 412,908 + Pf1N1B4_5964 0.59 +2.1 412,908 + Pf1N1B4_5964 0.59 +0.4 412,908 + Pf1N1B4_5964 0.59 +0.1 412,908 + Pf1N1B4_5964 0.59 +1.8 412,908 + Pf1N1B4_5964 0.59 -0.2 412,909 - -0.1 412,909 - Pf1N1B4_5964 0.59 -1.9 412,912 + Pf1N1B4_5964 0.60 +0.1 412,913 - Pf1N1B4_5964 0.60 +0.1 412,913 - Pf1N1B4_5964 0.60 -0.6 412,913 - Pf1N1B4_5964 0.60 +0.3 412,913 - Pf1N1B4_5964 0.60 +1.2 412,944 + Pf1N1B4_5964 0.63 +0.4 412,944 + Pf1N1B4_5964 0.63 +0.3 412,946 + +1.9 412,946 + Pf1N1B4_5964 0.63 -2.8 412,946 + Pf1N1B4_5964 0.63 +1.0 412,947 - Pf1N1B4_5964 0.64 -1.4 412,947 - Pf1N1B4_5964 0.64 +1.6 412,947 - Pf1N1B4_5964 0.64 +2.7 413,170 + Pf1N1B4_5964 0.87 -2.1 413,171 - Pf1N1B4_5964 0.88 +1.0 413,171 - Pf1N1B4_5964 0.88 +0.7 413,221 - +0.9 413,595 + Pf1N1B4_5965 0.27 +1.3 413,595 + Pf1N1B4_5965 0.27 +1.1 413,595 + Pf1N1B4_5965 0.27 -2.0 413,595 + Pf1N1B4_5965 0.27 -1.3 413,894 - Pf1N1B4_5965 0.54 +0.3 413,958 - Pf1N1B4_5965 0.60 +2.3 413,976 + Pf1N1B4_5965 0.62 +1.0 413,977 - +1.1 414,023 + Pf1N1B4_5965 0.66 +2.8 414,138 + Pf1N1B4_5965 0.76 -2.9 414,138 + Pf1N1B4_5965 0.76 +0.5 414,139 - Pf1N1B4_5965 0.76 +0.7 414,391 - -0.6 414,918 + Pf1N1B4_5967 0.23 +0.8 414,919 - Pf1N1B4_5967 0.23 +0.6 414,955 + Pf1N1B4_5967 0.26 -1.4 414,956 - Pf1N1B4_5967 0.26 +0.9 414,956 - Pf1N1B4_5967 0.26 -2.2 414,956 - Pf1N1B4_5967 0.26 -0.1
Or see this region's nucleotide sequence