Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5935 and Pf1N1B4_5936 are separated by 210 nucleotides Pf1N1B4_5936 and Pf1N1B4_5937 are separated by 426 nucleotides Pf1N1B4_5937 and Pf1N1B4_5938 overlap by 4 nucleotides Pf1N1B4_5938 and Pf1N1B4_5939 are separated by 45 nucleotides
Pf1N1B4_5935 - Polysaccharide export lipoprotein Wza, at 380,161 to 381,150
_5935
Pf1N1B4_5936 - hypothetical protein, at 381,361 to 381,633
_5936
Pf1N1B4_5937 - dTDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13), at 382,060 to 382,599
_5937
Pf1N1B4_5938 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 382,596 to 383,474
_5938
Pf1N1B4_5939 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 383,520 to 384,587
_5939
Position (kb) 382
383 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 381.363 kb on - strand at 381.363 kb on - strand at 381.363 kb on - strand at 381.659 kb on - strand at 381.666 kb on - strand at 381.675 kb on - strand at 381.675 kb on - strand at 381.675 kb on - strand at 381.706 kb on - strand at 381.706 kb on - strand at 381.742 kb on - strand at 381.742 kb on - strand at 381.772 kb on - strand at 381.774 kb on - strand at 381.774 kb on - strand at 381.945 kb on - strand at 381.986 kb on - strand at 381.986 kb on - strand at 381.986 kb on - strand at 382.012 kb on - strand at 382.012 kb on - strand at 382.037 kb on - strand at 382.037 kb on - strand at 382.041 kb on - strand at 382.041 kb on - strand at 382.043 kb on - strand at 382.043 kb on - strand at 382.051 kb on - strand at 382.118 kb on - strand, within Pf1N1B4_5937 at 382.194 kb on - strand, within Pf1N1B4_5937 at 382.194 kb on - strand, within Pf1N1B4_5937 at 382.194 kb on - strand, within Pf1N1B4_5937 at 382.257 kb on - strand, within Pf1N1B4_5937 at 382.355 kb on - strand, within Pf1N1B4_5937 at 382.512 kb on - strand, within Pf1N1B4_5937 at 382.568 kb on - strand at 382.614 kb on - strand at 382.865 kb on - strand, within Pf1N1B4_5938 at 382.865 kb on - strand, within Pf1N1B4_5938 at 382.949 kb on - strand, within Pf1N1B4_5938 at 382.949 kb on - strand, within Pf1N1B4_5938 at 382.955 kb on - strand, within Pf1N1B4_5938 at 382.955 kb on - strand, within Pf1N1B4_5938 at 382.955 kb on - strand, within Pf1N1B4_5938 at 383.141 kb on - strand, within Pf1N1B4_5938 at 383.174 kb on + strand, within Pf1N1B4_5938 at 383.175 kb on - strand, within Pf1N1B4_5938 at 383.322 kb on - strand, within Pf1N1B4_5938 at 383.322 kb on - strand at 383.329 kb on - strand, within Pf1N1B4_5938 at 383.356 kb on - strand at 383.372 kb on - strand, within Pf1N1B4_5938 at 383.372 kb on - strand, within Pf1N1B4_5938 at 383.372 kb on - strand, within Pf1N1B4_5938 at 383.390 kb on - strand at 383.413 kb on - strand at 383.427 kb on - strand at 383.597 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 381,363 - -0.5 381,363 - -0.8 381,363 - +0.8 381,659 - +2.4 381,666 - -1.4 381,675 - +0.7 381,675 - +1.9 381,675 - +0.2 381,706 - +0.5 381,706 - -0.1 381,742 - +1.9 381,742 - -0.2 381,772 - -0.6 381,774 - +0.9 381,774 - -0.9 381,945 - +0.7 381,986 - +1.6 381,986 - +0.8 381,986 - +0.9 382,012 - +0.6 382,012 - -1.2 382,037 - -0.5 382,037 - +1.1 382,041 - +0.7 382,041 - +1.1 382,043 - +1.3 382,043 - +0.9 382,051 - -1.2 382,118 - Pf1N1B4_5937 0.11 +0.1 382,194 - Pf1N1B4_5937 0.25 +0.7 382,194 - Pf1N1B4_5937 0.25 +0.0 382,194 - Pf1N1B4_5937 0.25 +1.0 382,257 - Pf1N1B4_5937 0.36 +0.3 382,355 - Pf1N1B4_5937 0.55 -0.6 382,512 - Pf1N1B4_5937 0.84 -0.9 382,568 - -0.8 382,614 - +0.1 382,865 - Pf1N1B4_5938 0.31 +1.6 382,865 - Pf1N1B4_5938 0.31 -1.4 382,949 - Pf1N1B4_5938 0.40 +0.5 382,949 - Pf1N1B4_5938 0.40 +0.4 382,955 - Pf1N1B4_5938 0.41 -0.5 382,955 - Pf1N1B4_5938 0.41 +1.1 382,955 - Pf1N1B4_5938 0.41 -1.6 383,141 - Pf1N1B4_5938 0.62 +1.6 383,174 + Pf1N1B4_5938 0.66 -1.4 383,175 - Pf1N1B4_5938 0.66 -0.0 383,322 - Pf1N1B4_5938 0.83 -1.4 383,322 - +2.9 383,329 - Pf1N1B4_5938 0.83 +1.1 383,356 - +2.4 383,372 - Pf1N1B4_5938 0.88 -0.8 383,372 - Pf1N1B4_5938 0.88 -1.6 383,372 - Pf1N1B4_5938 0.88 -0.9 383,390 - +1.5 383,413 - -3.0 383,427 - -0.5 383,597 - -0.8
Or see this region's nucleotide sequence