Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5884 and Pf1N1B4_5885 overlap by 4 nucleotides Pf1N1B4_5885 and Pf1N1B4_5886 overlap by 4 nucleotides Pf1N1B4_5886 and Pf1N1B4_5887 are separated by 0 nucleotides
Pf1N1B4_5884 - UDP-glucose dehydrogenase (EC 1.1.1.22), at 327,315 to 328,667
_5884
Pf1N1B4_5885 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 328,664 to 329,722
_5885
Pf1N1B4_5886 - Undecaprenyl-phosphate galactosephosphotransferase (EC 2.7.8.6), at 329,719 to 330,465
_5886
Pf1N1B4_5887 - Tyrosine-protein kinase Wzc (EC 2.7.10.2), at 330,466 to 331,110
_5887
Position (kb) 328
329
330 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 327.879 kb on + strand, within Pf1N1B4_5884 at 327.880 kb on - strand at 328.121 kb on + strand, within Pf1N1B4_5884 at 328.266 kb on - strand, within Pf1N1B4_5884 at 328.381 kb on - strand, within Pf1N1B4_5884 at 328.417 kb on - strand, within Pf1N1B4_5884 at 328.597 kb on + strand at 328.597 kb on + strand at 328.598 kb on - strand at 328.671 kb on + strand at 328.671 kb on + strand at 328.744 kb on - strand at 328.773 kb on - strand, within Pf1N1B4_5885 at 328.773 kb on - strand, within Pf1N1B4_5885 at 328.938 kb on - strand, within Pf1N1B4_5885 at 328.962 kb on - strand, within Pf1N1B4_5885 at 329.036 kb on + strand, within Pf1N1B4_5885 at 329.036 kb on + strand at 329.036 kb on + strand, within Pf1N1B4_5885 at 329.121 kb on - strand, within Pf1N1B4_5885 at 329.162 kb on + strand at 329.162 kb on + strand, within Pf1N1B4_5885 at 329.162 kb on + strand, within Pf1N1B4_5885 at 329.163 kb on - strand, within Pf1N1B4_5885 at 329.163 kb on - strand, within Pf1N1B4_5885 at 329.324 kb on + strand, within Pf1N1B4_5885 at 329.324 kb on + strand, within Pf1N1B4_5885 at 329.325 kb on - strand, within Pf1N1B4_5885 at 329.325 kb on - strand, within Pf1N1B4_5885 at 329.442 kb on - strand, within Pf1N1B4_5885 at 329.442 kb on - strand, within Pf1N1B4_5885 at 329.481 kb on - strand, within Pf1N1B4_5885 at 329.660 kb on + strand at 329.661 kb on - strand at 329.663 kb on + strand at 330.031 kb on + strand at 330.031 kb on + strand, within Pf1N1B4_5886 at 330.032 kb on - strand, within Pf1N1B4_5886 at 330.266 kb on - strand, within Pf1N1B4_5886 at 330.298 kb on + strand, within Pf1N1B4_5886 at 330.379 kb on + strand, within Pf1N1B4_5886 at 330.380 kb on - strand, within Pf1N1B4_5886 at 330.380 kb on - strand, within Pf1N1B4_5886 at 330.380 kb on - strand, within Pf1N1B4_5886
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 327,879 + Pf1N1B4_5884 0.42 +0.6 327,880 - +0.1 328,121 + Pf1N1B4_5884 0.60 -1.6 328,266 - Pf1N1B4_5884 0.70 +0.5 328,381 - Pf1N1B4_5884 0.79 -0.8 328,417 - Pf1N1B4_5884 0.81 +0.4 328,597 + -1.4 328,597 + +0.2 328,598 - -1.5 328,671 + +2.1 328,671 + +0.2 328,744 - +0.8 328,773 - Pf1N1B4_5885 0.10 +2.1 328,773 - Pf1N1B4_5885 0.10 +1.2 328,938 - Pf1N1B4_5885 0.26 -1.1 328,962 - Pf1N1B4_5885 0.28 +1.2 329,036 + Pf1N1B4_5885 0.35 -0.1 329,036 + -0.4 329,036 + Pf1N1B4_5885 0.35 +3.1 329,121 - Pf1N1B4_5885 0.43 -0.3 329,162 + -0.9 329,162 + Pf1N1B4_5885 0.47 +0.8 329,162 + Pf1N1B4_5885 0.47 -0.5 329,163 - Pf1N1B4_5885 0.47 +2.7 329,163 - Pf1N1B4_5885 0.47 +0.3 329,324 + Pf1N1B4_5885 0.62 +0.1 329,324 + Pf1N1B4_5885 0.62 -2.4 329,325 - Pf1N1B4_5885 0.62 +1.1 329,325 - Pf1N1B4_5885 0.62 +1.7 329,442 - Pf1N1B4_5885 0.73 +0.1 329,442 - Pf1N1B4_5885 0.73 -0.9 329,481 - Pf1N1B4_5885 0.77 +1.2 329,660 + +2.1 329,661 - +0.6 329,663 + +0.4 330,031 + -0.4 330,031 + Pf1N1B4_5886 0.42 +0.3 330,032 - Pf1N1B4_5886 0.42 +1.2 330,266 - Pf1N1B4_5886 0.73 +0.8 330,298 + Pf1N1B4_5886 0.78 -1.3 330,379 + Pf1N1B4_5886 0.88 -1.6 330,380 - Pf1N1B4_5886 0.88 +0.7 330,380 - Pf1N1B4_5886 0.88 +0.9 330,380 - Pf1N1B4_5886 0.88 +0.0
Or see this region's nucleotide sequence