Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5856 and Pf1N1B4_5857 are separated by 61 nucleotides Pf1N1B4_5857 and Pf1N1B4_5858 are separated by 340 nucleotides
Pf1N1B4_5856 - Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109), at 288,849 to 289,880
_5856
Pf1N1B4_5857 - Arginine N-succinyltransferase (EC 2.3.1.109), at 289,942 to 290,976
_5857
Pf1N1B4_5858 - carboxyphosphonoenolpyruvate phosphonomutase, putative, at 291,317 to 292,186
_5858
Position (kb) 289
290
291 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 289.023 kb on + strand, within Pf1N1B4_5856 at 289.023 kb on + strand, within Pf1N1B4_5856 at 289.024 kb on - strand, within Pf1N1B4_5856 at 289.024 kb on - strand, within Pf1N1B4_5856 at 289.209 kb on - strand, within Pf1N1B4_5856 at 289.209 kb on - strand, within Pf1N1B4_5856 at 289.267 kb on + strand, within Pf1N1B4_5856 at 289.267 kb on + strand, within Pf1N1B4_5856 at 289.267 kb on + strand, within Pf1N1B4_5856 at 289.347 kb on + strand, within Pf1N1B4_5856 at 289.413 kb on + strand at 289.596 kb on + strand, within Pf1N1B4_5856 at 289.596 kb on + strand, within Pf1N1B4_5856 at 289.596 kb on + strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.600 kb on + strand, within Pf1N1B4_5856 at 289.601 kb on - strand, within Pf1N1B4_5856 at 289.601 kb on - strand, within Pf1N1B4_5856 at 289.731 kb on + strand, within Pf1N1B4_5856 at 289.731 kb on + strand, within Pf1N1B4_5856 at 289.732 kb on - strand, within Pf1N1B4_5856 at 289.732 kb on - strand, within Pf1N1B4_5856 at 289.732 kb on - strand, within Pf1N1B4_5856 at 289.916 kb on + strand at 289.916 kb on + strand at 289.916 kb on + strand at 290.057 kb on + strand at 290.260 kb on - strand, within Pf1N1B4_5857 at 290.340 kb on + strand, within Pf1N1B4_5857 at 290.681 kb on - strand, within Pf1N1B4_5857 at 291.153 kb on - strand at 291.153 kb on - strand at 291.247 kb on - strand at 291.302 kb on - strand at 291.317 kb on - strand at 291.350 kb on - strand at 291.383 kb on + strand at 291.931 kb on - strand, within Pf1N1B4_5858 at 291.950 kb on + strand, within Pf1N1B4_5858 at 291.951 kb on - strand, within Pf1N1B4_5858
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 289,023 + Pf1N1B4_5856 0.17 -2.8 289,023 + Pf1N1B4_5856 0.17 -2.4 289,024 - Pf1N1B4_5856 0.17 +0.0 289,024 - Pf1N1B4_5856 0.17 -2.2 289,209 - Pf1N1B4_5856 0.35 -0.9 289,209 - Pf1N1B4_5856 0.35 -1.5 289,267 + Pf1N1B4_5856 0.41 -1.4 289,267 + Pf1N1B4_5856 0.41 +0.7 289,267 + Pf1N1B4_5856 0.41 +1.6 289,347 + Pf1N1B4_5856 0.48 -0.9 289,413 + +2.4 289,596 + Pf1N1B4_5856 0.72 +1.3 289,596 + Pf1N1B4_5856 0.72 +1.8 289,596 + Pf1N1B4_5856 0.72 -0.5 289,597 - Pf1N1B4_5856 0.72 +1.6 289,597 - Pf1N1B4_5856 0.72 -0.9 289,597 - Pf1N1B4_5856 0.72 +0.4 289,597 - Pf1N1B4_5856 0.72 +1.0 289,597 - Pf1N1B4_5856 0.72 +1.9 289,600 + Pf1N1B4_5856 0.73 -0.9 289,601 - Pf1N1B4_5856 0.73 +1.6 289,601 - Pf1N1B4_5856 0.73 +0.8 289,731 + Pf1N1B4_5856 0.85 +0.9 289,731 + Pf1N1B4_5856 0.85 -0.6 289,732 - Pf1N1B4_5856 0.86 -1.1 289,732 - Pf1N1B4_5856 0.86 -0.0 289,732 - Pf1N1B4_5856 0.86 +0.7 289,916 + -0.2 289,916 + -0.2 289,916 + +0.2 290,057 + -0.9 290,260 - Pf1N1B4_5857 0.31 +1.6 290,340 + Pf1N1B4_5857 0.38 +0.0 290,681 - Pf1N1B4_5857 0.71 +1.0 291,153 - +0.9 291,153 - +0.6 291,247 - +0.6 291,302 - -0.1 291,317 - +1.0 291,350 - +0.6 291,383 + -0.3 291,931 - Pf1N1B4_5858 0.71 +0.2 291,950 + Pf1N1B4_5858 0.73 +0.5 291,951 - Pf1N1B4_5858 0.73 -2.5
Or see this region's nucleotide sequence