Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5855 and Pf1N1B4_5856 are separated by 119 nucleotides Pf1N1B4_5856 and Pf1N1B4_5857 are separated by 61 nucleotides
Pf1N1B4_5855 - Putrescine importer, at 287,380 to 288,729
_5855
Pf1N1B4_5856 - Arginine N-succinyltransferase, alpha subunit (EC 2.3.1.109), at 288,849 to 289,880
_5856
Pf1N1B4_5857 - Arginine N-succinyltransferase (EC 2.3.1.109), at 289,942 to 290,976
_5857
Position (kb)
288
289
290 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 287.852 kb on + strand, within Pf1N1B4_5855 at 287.891 kb on - strand, within Pf1N1B4_5855 at 287.922 kb on + strand, within Pf1N1B4_5855 at 287.950 kb on + strand, within Pf1N1B4_5855 at 287.951 kb on - strand, within Pf1N1B4_5855 at 287.951 kb on - strand, within Pf1N1B4_5855 at 287.951 kb on - strand, within Pf1N1B4_5855 at 288.194 kb on - strand, within Pf1N1B4_5855 at 288.382 kb on + strand, within Pf1N1B4_5855 at 288.394 kb on + strand, within Pf1N1B4_5855 at 288.394 kb on + strand, within Pf1N1B4_5855 at 288.395 kb on - strand, within Pf1N1B4_5855 at 288.395 kb on - strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.520 kb on + strand, within Pf1N1B4_5855 at 288.521 kb on - strand, within Pf1N1B4_5855 at 288.521 kb on - strand, within Pf1N1B4_5855 at 288.523 kb on + strand, within Pf1N1B4_5855 at 288.607 kb on + strand at 288.607 kb on + strand at 288.607 kb on + strand at 288.607 kb on + strand at 288.607 kb on + strand at 288.607 kb on + strand at 288.607 kb on + strand at 288.608 kb on - strand at 288.608 kb on - strand at 288.667 kb on + strand at 288.667 kb on + strand at 288.668 kb on - strand at 288.668 kb on - strand at 288.671 kb on + strand at 288.671 kb on + strand at 288.671 kb on + strand at 288.671 kb on + strand at 288.671 kb on + strand at 288.671 kb on + strand at 288.799 kb on + strand at 288.799 kb on + strand at 288.842 kb on + strand at 288.842 kb on + strand at 288.842 kb on + strand at 288.842 kb on + strand at 288.842 kb on + strand at 288.842 kb on + strand at 288.843 kb on - strand at 288.859 kb on + strand at 289.023 kb on + strand, within Pf1N1B4_5856 at 289.023 kb on + strand, within Pf1N1B4_5856 at 289.024 kb on - strand, within Pf1N1B4_5856 at 289.024 kb on - strand, within Pf1N1B4_5856 at 289.209 kb on - strand, within Pf1N1B4_5856 at 289.209 kb on - strand, within Pf1N1B4_5856 at 289.267 kb on + strand, within Pf1N1B4_5856 at 289.267 kb on + strand, within Pf1N1B4_5856 at 289.267 kb on + strand, within Pf1N1B4_5856 at 289.347 kb on + strand, within Pf1N1B4_5856 at 289.413 kb on + strand at 289.596 kb on + strand, within Pf1N1B4_5856 at 289.596 kb on + strand, within Pf1N1B4_5856 at 289.596 kb on + strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.597 kb on - strand, within Pf1N1B4_5856 at 289.600 kb on + strand, within Pf1N1B4_5856 at 289.601 kb on - strand, within Pf1N1B4_5856 at 289.601 kb on - strand, within Pf1N1B4_5856 at 289.731 kb on + strand, within Pf1N1B4_5856 at 289.731 kb on + strand, within Pf1N1B4_5856 at 289.732 kb on - strand, within Pf1N1B4_5856 at 289.732 kb on - strand, within Pf1N1B4_5856 at 289.732 kb on - strand, within Pf1N1B4_5856 at 289.916 kb on + strand at 289.916 kb on + strand at 289.916 kb on + strand at 290.057 kb on + strand at 290.260 kb on - strand, within Pf1N1B4_5857 at 290.340 kb on + strand, within Pf1N1B4_5857 at 290.681 kb on - strand, within Pf1N1B4_5857
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 287,852 + Pf1N1B4_5855 0.35 +1.6 287,891 - Pf1N1B4_5855 0.38 +1.4 287,922 + Pf1N1B4_5855 0.40 +1.2 287,950 + Pf1N1B4_5855 0.42 -1.6 287,951 - Pf1N1B4_5855 0.42 +0.9 287,951 - Pf1N1B4_5855 0.42 +0.9 287,951 - Pf1N1B4_5855 0.42 +0.9 288,194 - Pf1N1B4_5855 0.60 -0.6 288,382 + Pf1N1B4_5855 0.74 -1.5 288,394 + Pf1N1B4_5855 0.75 -2.1 288,394 + Pf1N1B4_5855 0.75 -0.1 288,395 - Pf1N1B4_5855 0.75 -1.3 288,395 - Pf1N1B4_5855 0.75 -0.4 288,520 + Pf1N1B4_5855 0.84 +1.0 288,520 + Pf1N1B4_5855 0.84 +1.3 288,520 + Pf1N1B4_5855 0.84 -1.4 288,520 + Pf1N1B4_5855 0.84 +1.4 288,520 + Pf1N1B4_5855 0.84 -1.6 288,520 + Pf1N1B4_5855 0.84 -0.9 288,520 + Pf1N1B4_5855 0.84 -2.4 288,521 - Pf1N1B4_5855 0.85 +0.3 288,521 - Pf1N1B4_5855 0.85 -2.1 288,523 + Pf1N1B4_5855 0.85 -1.6 288,607 + +0.9 288,607 + -1.1 288,607 + -0.5 288,607 + -1.1 288,607 + -2.6 288,607 + +0.9 288,607 + +1.1 288,608 - +2.2 288,608 - -0.9 288,667 + +0.6 288,667 + +0.8 288,668 - +0.1 288,668 - -0.1 288,671 + -1.4 288,671 + -2.2 288,671 + +0.8 288,671 + -1.2 288,671 + +0.3 288,671 + -0.6 288,799 + +0.5 288,799 + -1.4 288,842 + +0.1 288,842 + +0.2 288,842 + +0.4 288,842 + +1.5 288,842 + -0.3 288,842 + -3.4 288,843 - +0.2 288,859 + +0.1 289,023 + Pf1N1B4_5856 0.17 -2.8 289,023 + Pf1N1B4_5856 0.17 -2.4 289,024 - Pf1N1B4_5856 0.17 +0.0 289,024 - Pf1N1B4_5856 0.17 -2.2 289,209 - Pf1N1B4_5856 0.35 -0.9 289,209 - Pf1N1B4_5856 0.35 -1.5 289,267 + Pf1N1B4_5856 0.41 -1.4 289,267 + Pf1N1B4_5856 0.41 +0.7 289,267 + Pf1N1B4_5856 0.41 +1.6 289,347 + Pf1N1B4_5856 0.48 -0.9 289,413 + +2.4 289,596 + Pf1N1B4_5856 0.72 +1.3 289,596 + Pf1N1B4_5856 0.72 +1.8 289,596 + Pf1N1B4_5856 0.72 -0.5 289,597 - Pf1N1B4_5856 0.72 +1.6 289,597 - Pf1N1B4_5856 0.72 -0.9 289,597 - Pf1N1B4_5856 0.72 +0.4 289,597 - Pf1N1B4_5856 0.72 +1.0 289,597 - Pf1N1B4_5856 0.72 +1.9 289,600 + Pf1N1B4_5856 0.73 -0.9 289,601 - Pf1N1B4_5856 0.73 +1.6 289,601 - Pf1N1B4_5856 0.73 +0.8 289,731 + Pf1N1B4_5856 0.85 +0.9 289,731 + Pf1N1B4_5856 0.85 -0.6 289,732 - Pf1N1B4_5856 0.86 -1.1 289,732 - Pf1N1B4_5856 0.86 -0.0 289,732 - Pf1N1B4_5856 0.86 +0.7 289,916 + -0.2 289,916 + -0.2 289,916 + +0.2 290,057 + -0.9 290,260 - Pf1N1B4_5857 0.31 +1.6 290,340 + Pf1N1B4_5857 0.38 +0.0 290,681 - Pf1N1B4_5857 0.71 +1.0
Or see this region's nucleotide sequence