Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5842 and Pf1N1B4_5843 are separated by 11 nucleotides Pf1N1B4_5843 and Pf1N1B4_5844 are separated by 164 nucleotides Pf1N1B4_5844 and Pf1N1B4_5845 are separated by 322 nucleotides
Pf1N1B4_5842 - Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase), at 277,098 to 277,685
_5842
Pf1N1B4_5843 - phospholipase/carboxylesterase family protein, at 277,697 to 278,413
_5843
Pf1N1B4_5844 - Arginine/ornithine ABC transporter, periplasmic arginine/ornithine binding protein, at 278,578 to 279,357
_5844
Pf1N1B4_5845 - GMP synthase - Glutamine amidotransferase domain, at 279,680 to 280,321
_5845
Position (kb) 278
279
280 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 277.683 kb on + strand at 277.683 kb on + strand at 277.684 kb on - strand at 278.034 kb on - strand, within Pf1N1B4_5843 at 278.056 kb on - strand, within Pf1N1B4_5843 at 278.205 kb on + strand, within Pf1N1B4_5843 at 278.205 kb on + strand, within Pf1N1B4_5843 at 278.205 kb on + strand, within Pf1N1B4_5843 at 278.205 kb on + strand, within Pf1N1B4_5843 at 278.206 kb on - strand, within Pf1N1B4_5843 at 278.211 kb on + strand, within Pf1N1B4_5843 at 278.325 kb on + strand at 278.413 kb on + strand at 278.413 kb on + strand at 278.413 kb on + strand at 278.413 kb on + strand at 278.413 kb on + strand at 278.413 kb on + strand at 278.413 kb on + strand at 278.414 kb on - strand at 278.453 kb on + strand at 278.521 kb on + strand at 278.521 kb on + strand at 278.521 kb on + strand at 278.521 kb on + strand at 278.521 kb on + strand at 278.570 kb on - strand at 278.577 kb on - strand at 278.577 kb on - strand at 278.579 kb on + strand at 278.580 kb on - strand at 278.606 kb on + strand at 278.606 kb on + strand at 278.607 kb on - strand at 278.607 kb on - strand at 278.627 kb on + strand at 278.628 kb on - strand at 278.726 kb on + strand, within Pf1N1B4_5844 at 278.726 kb on + strand at 278.726 kb on + strand, within Pf1N1B4_5844 at 278.727 kb on - strand, within Pf1N1B4_5844 at 278.741 kb on + strand, within Pf1N1B4_5844 at 278.742 kb on - strand, within Pf1N1B4_5844 at 278.742 kb on - strand, within Pf1N1B4_5844 at 278.742 kb on - strand, within Pf1N1B4_5844 at 278.753 kb on - strand, within Pf1N1B4_5844 at 279.033 kb on - strand, within Pf1N1B4_5844 at 279.033 kb on - strand at 279.033 kb on - strand, within Pf1N1B4_5844 at 279.035 kb on + strand at 279.035 kb on + strand at 279.035 kb on + strand, within Pf1N1B4_5844 at 279.036 kb on - strand, within Pf1N1B4_5844 at 279.359 kb on + strand at 279.359 kb on + strand at 279.360 kb on - strand at 279.360 kb on - strand at 279.362 kb on - strand at 279.362 kb on - strand at 279.483 kb on + strand at 279.483 kb on + strand at 279.484 kb on - strand at 279.484 kb on - strand at 279.487 kb on + strand at 279.489 kb on + strand at 279.516 kb on - strand at 279.840 kb on - strand, within Pf1N1B4_5845
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 277,683 + -1.0 277,683 + +0.4 277,684 - +1.2 278,034 - Pf1N1B4_5843 0.47 -0.6 278,056 - Pf1N1B4_5843 0.50 -1.5 278,205 + Pf1N1B4_5843 0.71 +0.9 278,205 + Pf1N1B4_5843 0.71 -2.2 278,205 + Pf1N1B4_5843 0.71 -1.9 278,205 + Pf1N1B4_5843 0.71 +0.5 278,206 - Pf1N1B4_5843 0.71 +0.2 278,211 + Pf1N1B4_5843 0.72 -0.8 278,325 + -0.1 278,413 + -1.9 278,413 + +0.3 278,413 + +0.3 278,413 + -1.1 278,413 + -1.4 278,413 + +1.5 278,413 + -2.0 278,414 - -3.4 278,453 + +1.7 278,521 + -0.8 278,521 + -0.5 278,521 + +0.3 278,521 + -0.1 278,521 + -0.5 278,570 - -0.4 278,577 - +0.5 278,577 - +0.8 278,579 + -0.5 278,580 - +0.2 278,606 + +0.6 278,606 + -0.8 278,607 - -0.2 278,607 - -0.9 278,627 + +1.8 278,628 - +0.4 278,726 + Pf1N1B4_5844 0.19 +1.7 278,726 + +1.8 278,726 + Pf1N1B4_5844 0.19 -0.6 278,727 - Pf1N1B4_5844 0.19 +0.5 278,741 + Pf1N1B4_5844 0.21 +1.5 278,742 - Pf1N1B4_5844 0.21 -0.7 278,742 - Pf1N1B4_5844 0.21 +0.2 278,742 - Pf1N1B4_5844 0.21 +1.2 278,753 - Pf1N1B4_5844 0.22 +2.5 279,033 - Pf1N1B4_5844 0.58 +0.8 279,033 - -1.2 279,033 - Pf1N1B4_5844 0.58 +0.8 279,035 + -0.4 279,035 + +0.8 279,035 + Pf1N1B4_5844 0.59 +0.4 279,036 - Pf1N1B4_5844 0.59 +1.8 279,359 + -1.5 279,359 + +0.8 279,360 - -1.0 279,360 - +2.9 279,362 - +0.9 279,362 - +0.9 279,483 + -0.8 279,483 + -0.4 279,484 - +0.0 279,484 - +1.2 279,487 + +0.0 279,489 + -0.3 279,516 - -0.2 279,840 - Pf1N1B4_5845 0.25 -0.1
Or see this region's nucleotide sequence