Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5821 and Pf1N1B4_5822 are separated by 290 nucleotides Pf1N1B4_5822 and Pf1N1B4_5823 overlap by 1 nucleotides Pf1N1B4_5823 and Pf1N1B4_5824 are separated by 5 nucleotides
Pf1N1B4_5821 - Permease of the drug/metabolite transporter (DMT) superfamily, at 254,762 to 255,790
_5821
Pf1N1B4_5822 - Na(+) H(+) antiporter subunit A / Na(+) H(+) antiporter subunit B, at 256,081 to 258,957
_5822
Pf1N1B4_5823 - Na(+) H(+) antiporter subunit C, at 258,957 to 259,301
_5823
Pf1N1B4_5824 - Na(+) H(+) antiporter subunit D, at 259,307 to 260,983
_5824
Position (kb) 256
257
258
259 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 255.188 kb on + strand, within Pf1N1B4_5821 at 255.188 kb on + strand, within Pf1N1B4_5821 at 255.188 kb on + strand, within Pf1N1B4_5821 at 255.188 kb on + strand, within Pf1N1B4_5821 at 255.392 kb on + strand at 255.392 kb on + strand, within Pf1N1B4_5821 at 255.392 kb on + strand, within Pf1N1B4_5821 at 255.392 kb on + strand, within Pf1N1B4_5821 at 255.393 kb on - strand, within Pf1N1B4_5821 at 255.411 kb on + strand, within Pf1N1B4_5821 at 255.411 kb on + strand, within Pf1N1B4_5821 at 255.412 kb on - strand, within Pf1N1B4_5821 at 255.611 kb on + strand, within Pf1N1B4_5821 at 255.611 kb on + strand, within Pf1N1B4_5821 at 255.945 kb on - strand at 255.991 kb on + strand at 255.991 kb on + strand at 256.018 kb on + strand at 256.026 kb on + strand at 256.037 kb on + strand at 256.037 kb on + strand at 256.044 kb on - strand at 256.321 kb on + strand at 256.321 kb on + strand at 256.322 kb on - strand at 256.322 kb on - strand at 256.322 kb on - strand at 256.507 kb on + strand, within Pf1N1B4_5822 at 256.507 kb on + strand, within Pf1N1B4_5822 at 256.508 kb on - strand, within Pf1N1B4_5822 at 256.588 kb on + strand, within Pf1N1B4_5822 at 256.840 kb on + strand, within Pf1N1B4_5822 at 256.840 kb on + strand, within Pf1N1B4_5822 at 256.840 kb on + strand, within Pf1N1B4_5822 at 256.841 kb on - strand, within Pf1N1B4_5822 at 256.866 kb on + strand, within Pf1N1B4_5822 at 256.867 kb on - strand, within Pf1N1B4_5822 at 257.227 kb on - strand, within Pf1N1B4_5822 at 257.299 kb on + strand, within Pf1N1B4_5822 at 257.412 kb on + strand at 257.412 kb on + strand, within Pf1N1B4_5822 at 257.507 kb on - strand, within Pf1N1B4_5822 at 257.908 kb on + strand, within Pf1N1B4_5822 at 258.223 kb on + strand, within Pf1N1B4_5822 at 258.619 kb on + strand, within Pf1N1B4_5822 at 258.619 kb on + strand, within Pf1N1B4_5822 at 258.619 kb on + strand, within Pf1N1B4_5822 at 258.620 kb on - strand, within Pf1N1B4_5822 at 258.829 kb on + strand at 258.898 kb on - strand at 259.065 kb on + strand, within Pf1N1B4_5823 at 259.292 kb on + strand at 259.496 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.799 kb on + strand, within Pf1N1B4_5824 at 259.800 kb on - strand, within Pf1N1B4_5824 at 259.856 kb on + strand, within Pf1N1B4_5824
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 255,188 + Pf1N1B4_5821 0.41 +1.1 255,188 + Pf1N1B4_5821 0.41 +0.6 255,188 + Pf1N1B4_5821 0.41 -2.5 255,188 + Pf1N1B4_5821 0.41 -0.5 255,392 + +0.9 255,392 + Pf1N1B4_5821 0.61 -2.0 255,392 + Pf1N1B4_5821 0.61 -0.3 255,392 + Pf1N1B4_5821 0.61 +0.2 255,393 - Pf1N1B4_5821 0.61 -0.6 255,411 + Pf1N1B4_5821 0.63 +2.2 255,411 + Pf1N1B4_5821 0.63 -1.8 255,412 - Pf1N1B4_5821 0.63 +0.1 255,611 + Pf1N1B4_5821 0.83 +1.5 255,611 + Pf1N1B4_5821 0.83 +1.3 255,945 - -0.9 255,991 + +1.6 255,991 + +0.6 256,018 + -0.8 256,026 + -1.6 256,037 + -1.3 256,037 + -1.5 256,044 - -1.3 256,321 + -1.5 256,321 + -2.2 256,322 - +0.5 256,322 - -0.9 256,322 - -2.9 256,507 + Pf1N1B4_5822 0.15 -2.7 256,507 + Pf1N1B4_5822 0.15 -3.1 256,508 - Pf1N1B4_5822 0.15 -1.9 256,588 + Pf1N1B4_5822 0.18 -3.5 256,840 + Pf1N1B4_5822 0.26 -3.4 256,840 + Pf1N1B4_5822 0.26 -4.3 256,840 + Pf1N1B4_5822 0.26 +0.7 256,841 - Pf1N1B4_5822 0.26 -2.5 256,866 + Pf1N1B4_5822 0.27 -3.8 256,867 - Pf1N1B4_5822 0.27 -2.3 257,227 - Pf1N1B4_5822 0.40 -0.1 257,299 + Pf1N1B4_5822 0.42 -2.3 257,412 + -0.4 257,412 + Pf1N1B4_5822 0.46 -1.0 257,507 - Pf1N1B4_5822 0.50 -3.2 257,908 + Pf1N1B4_5822 0.64 -1.4 258,223 + Pf1N1B4_5822 0.74 -4.5 258,619 + Pf1N1B4_5822 0.88 +0.1 258,619 + Pf1N1B4_5822 0.88 -1.8 258,619 + Pf1N1B4_5822 0.88 -2.1 258,620 - Pf1N1B4_5822 0.88 -2.4 258,829 + -2.3 258,898 - +0.5 259,065 + Pf1N1B4_5823 0.31 -2.0 259,292 + -0.4 259,496 + Pf1N1B4_5824 0.11 -1.5 259,799 + Pf1N1B4_5824 0.29 -2.8 259,799 + Pf1N1B4_5824 0.29 -4.1 259,799 + Pf1N1B4_5824 0.29 -3.7 259,799 + -0.9 259,799 + Pf1N1B4_5824 0.29 +0.7 259,799 + Pf1N1B4_5824 0.29 -1.3 259,799 + Pf1N1B4_5824 0.29 -1.8 259,800 - Pf1N1B4_5824 0.29 -1.5 259,856 + Pf1N1B4_5824 0.33 -2.2
Or see this region's nucleotide sequence