Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_5044 and Pf1N1B4_5045 are separated by 278 nucleotides Pf1N1B4_5045 and Pf1N1B4_5046 overlap by 1 nucleotides Pf1N1B4_5046 and Pf1N1B4_5047 are separated by 184 nucleotides
Pf1N1B4_5044 - hypothetical protein, at 5,589,635 to 5,589,994
_5044
Pf1N1B4_5045 - Cytochrome oxidase biogenesis protein Sco1/SenC/PrrC, putative copper metallochaperone, at 5,590,273 to 5,590,878
_5045
Pf1N1B4_5046 - Copper metallochaperone, bacterial analog of Cox17 protein, at 5,590,878 to 5,591,351
_5046
Pf1N1B4_5047 - Integral membrane protein TerC, at 5,591,536 to 5,592,507
_5047
Position (kb)
5590
5591 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 5589.299 kb on - strand at 5589.299 kb on - strand at 5589.328 kb on + strand at 5589.329 kb on - strand at 5589.450 kb on + strand at 5589.548 kb on - strand at 5589.634 kb on + strand at 5589.634 kb on + strand at 5589.634 kb on + strand at 5589.635 kb on - strand at 5589.635 kb on - strand at 5589.775 kb on + strand at 5589.775 kb on + strand at 5589.979 kb on + strand at 5589.979 kb on + strand at 5589.979 kb on + strand at 5589.982 kb on + strand at 5589.983 kb on - strand at 5589.992 kb on + strand at 5589.993 kb on - strand at 5589.993 kb on - strand at 5590.253 kb on + strand at 5590.278 kb on + strand at 5590.278 kb on + strand at 5590.278 kb on + strand at 5590.278 kb on + strand at 5590.278 kb on + strand at 5590.278 kb on + strand at 5590.278 kb on + strand at 5590.279 kb on - strand at 5590.279 kb on - strand at 5590.279 kb on - strand at 5590.279 kb on - strand at 5590.279 kb on - strand at 5590.279 kb on - strand at 5590.285 kb on + strand at 5590.285 kb on + strand at 5590.285 kb on + strand at 5590.285 kb on + strand at 5590.285 kb on + strand at 5590.285 kb on + strand at 5590.286 kb on - strand at 5590.286 kb on - strand at 5590.376 kb on - strand, within Pf1N1B4_5045 at 5590.411 kb on - strand at 5590.672 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand, within Pf1N1B4_5045 at 5590.750 kb on + strand at 5590.751 kb on - strand, within Pf1N1B4_5045 at 5590.751 kb on - strand, within Pf1N1B4_5045 at 5590.751 kb on - strand, within Pf1N1B4_5045 at 5590.890 kb on + strand at 5590.890 kb on + strand at 5590.890 kb on + strand at 5590.891 kb on - strand at 5590.891 kb on - strand at 5590.891 kb on - strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.901 kb on + strand at 5590.902 kb on - strand at 5590.904 kb on + strand at 5590.905 kb on - strand at 5590.905 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 5,589,299 - +0.5 5,589,299 - +0.6 5,589,328 + +0.2 5,589,329 - -0.8 5,589,450 + +0.7 5,589,548 - -0.3 5,589,634 + -2.6 5,589,634 + +2.1 5,589,634 + -0.8 5,589,635 - -1.2 5,589,635 - +0.6 5,589,775 + -0.3 5,589,775 + -1.3 5,589,979 + -0.5 5,589,979 + -1.7 5,589,979 + +0.5 5,589,982 + -1.6 5,589,983 - -0.9 5,589,992 + -1.1 5,589,993 - -1.6 5,589,993 - -2.3 5,590,253 + +0.7 5,590,278 + -0.2 5,590,278 + +0.8 5,590,278 + +1.1 5,590,278 + +1.0 5,590,278 + +0.4 5,590,278 + +1.2 5,590,278 + +1.4 5,590,279 - +0.8 5,590,279 - +0.5 5,590,279 - +2.1 5,590,279 - +1.1 5,590,279 - -0.2 5,590,279 - +0.5 5,590,285 + +1.1 5,590,285 + +1.6 5,590,285 + +1.5 5,590,285 + +0.9 5,590,285 + -1.0 5,590,285 + +2.7 5,590,286 - +0.0 5,590,286 - -0.1 5,590,376 - Pf1N1B4_5045 0.17 +1.2 5,590,411 - -0.9 5,590,672 + Pf1N1B4_5045 0.66 +1.3 5,590,750 + Pf1N1B4_5045 0.79 -0.0 5,590,750 + Pf1N1B4_5045 0.79 -1.5 5,590,750 + Pf1N1B4_5045 0.79 +1.8 5,590,750 + Pf1N1B4_5045 0.79 +1.1 5,590,750 + +0.9 5,590,751 - Pf1N1B4_5045 0.79 +1.2 5,590,751 - Pf1N1B4_5045 0.79 +1.9 5,590,751 - Pf1N1B4_5045 0.79 +2.0 5,590,890 + +1.1 5,590,890 + +0.5 5,590,890 + +1.1 5,590,891 - +0.4 5,590,891 - -2.0 5,590,891 - +1.7 5,590,901 + -1.5 5,590,901 + +2.5 5,590,901 + +1.1 5,590,901 + +0.4 5,590,901 + +0.2 5,590,901 + +0.7 5,590,902 - +1.6 5,590,904 + -1.9 5,590,905 - +1.1 5,590,905 - +0.9
Or see this region's nucleotide sequence