Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_503

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_502 and Pf1N1B4_503 are separated by 2 nucleotidesPf1N1B4_503 and Pf1N1B4_504 are separated by 26 nucleotidesPf1N1B4_504 and Pf1N1B4_505 are separated by 465 nucleotidesPf1N1B4_505 and Pf1N1B4_506 are separated by 40 nucleotides Pf1N1B4_502 - Predicted ATPase with chaperone activity, associated with Flp pilus assembly, at 594,190 to 595,524 _502 Pf1N1B4_503 - Type IV prepilin peptidase TadV/CpaA, at 595,527 to 596,057 _503 Pf1N1B4_504 - Flp pilus assembly protein, pilin Flp, at 596,084 to 596,275 _504 Pf1N1B4_505 - hypothetical protein, at 596,741 to 596,926 _505 Pf1N1B4_506 - Transcriptional regulator, AraC family, at 596,967 to 597,551 _506 Position (kb) 595 596 597Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 594.607 kb on + strand, within Pf1N1B4_502at 594.881 kb on + strand, within Pf1N1B4_502at 594.881 kb on + strand, within Pf1N1B4_502at 594.881 kb on + strand, within Pf1N1B4_502at 594.881 kb on + strand, within Pf1N1B4_502at 594.881 kb on + strand, within Pf1N1B4_502at 594.882 kb on - strand, within Pf1N1B4_502at 594.882 kb on - strand, within Pf1N1B4_502at 594.968 kb on + strand, within Pf1N1B4_502at 594.968 kb on + strand, within Pf1N1B4_502at 594.968 kb on + strand, within Pf1N1B4_502at 594.969 kb on - strand, within Pf1N1B4_502at 594.969 kb on - strand, within Pf1N1B4_502at 594.969 kb on - strand, within Pf1N1B4_502at 594.969 kb on - strand, within Pf1N1B4_502at 594.995 kb on + strand, within Pf1N1B4_502at 594.996 kb on - strand, within Pf1N1B4_502at 594.996 kb on - strand, within Pf1N1B4_502at 594.996 kb on - strand, within Pf1N1B4_502at 595.060 kb on + strand, within Pf1N1B4_502at 595.142 kb on + strand, within Pf1N1B4_502at 595.143 kb on - strand, within Pf1N1B4_502at 595.146 kb on + strand, within Pf1N1B4_502at 595.147 kb on - strand, within Pf1N1B4_502at 595.147 kb on - strand, within Pf1N1B4_502at 595.175 kb on + strandat 595.175 kb on + strand, within Pf1N1B4_502at 595.176 kb on - strand, within Pf1N1B4_502at 595.277 kb on - strand, within Pf1N1B4_502at 595.277 kb on - strand, within Pf1N1B4_502at 595.280 kb on - strandat 595.280 kb on - strand, within Pf1N1B4_502at 595.496 kb on + strandat 595.496 kb on + strandat 595.496 kb on + strandat 595.497 kb on - strandat 595.507 kb on - strandat 595.529 kb on - strandat 595.561 kb on + strandat 595.561 kb on + strandat 595.643 kb on + strandat 595.645 kb on + strand, within Pf1N1B4_503at 595.645 kb on + strand, within Pf1N1B4_503at 595.645 kb on + strand, within Pf1N1B4_503at 595.645 kb on + strand, within Pf1N1B4_503at 595.645 kb on + strand, within Pf1N1B4_503at 595.645 kb on + strand, within Pf1N1B4_503at 595.783 kb on - strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strand, within Pf1N1B4_503at 595.843 kb on + strandat 595.844 kb on - strand, within Pf1N1B4_503at 595.844 kb on - strand, within Pf1N1B4_503at 595.844 kb on - strand, within Pf1N1B4_503at 595.844 kb on - strand, within Pf1N1B4_503at 595.844 kb on - strand, within Pf1N1B4_503at 595.844 kb on - strand, within Pf1N1B4_503at 595.844 kb on - strand, within Pf1N1B4_503at 595.884 kb on + strand, within Pf1N1B4_503at 595.885 kb on - strand, within Pf1N1B4_503at 595.918 kb on + strand, within Pf1N1B4_503at 595.918 kb on + strand, within Pf1N1B4_503at 595.918 kb on + strand, within Pf1N1B4_503at 595.918 kb on + strand, within Pf1N1B4_503at 595.919 kb on - strand, within Pf1N1B4_503at 595.950 kb on + strand, within Pf1N1B4_503at 596.034 kb on - strandat 596.071 kb on - strandat 596.071 kb on - strandat 596.071 kb on - strandat 596.074 kb on - strandat 596.074 kb on - strandat 596.082 kb on + strandat 596.082 kb on + strandat 596.083 kb on - strandat 596.083 kb on - strandat 596.085 kb on + strandat 596.086 kb on - strandat 596.092 kb on + strandat 596.092 kb on + strandat 596.093 kb on - strandat 596.202 kb on + strand, within Pf1N1B4_504at 596.258 kb on - strandat 596.258 kb on - strandat 596.344 kb on + strandat 596.344 kb on + strandat 596.344 kb on + strandat 596.345 kb on - strandat 596.389 kb on + strandat 596.389 kb on + strandat 596.390 kb on - strandat 596.417 kb on - strandat 596.551 kb on - strandat 596.641 kb on + strandat 596.641 kb on + strandat 596.641 kb on + strandat 596.641 kb on + strandat 596.641 kb on + strandat 596.642 kb on - strandat 596.642 kb on - strandat 596.644 kb on + strandat 596.644 kb on + strandat 596.645 kb on - strandat 596.678 kb on + strandat 596.678 kb on + strandat 596.988 kb on + strandat 596.988 kb on + strandat 596.988 kb on + strandat 596.989 kb on - strandat 596.989 kb on - strandat 596.989 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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594,607 + Pf1N1B4_502 0.31 +1.1
594,881 + Pf1N1B4_502 0.52 +1.0
594,881 + Pf1N1B4_502 0.52 -1.6
594,881 + Pf1N1B4_502 0.52 -0.1
594,881 + Pf1N1B4_502 0.52 -1.8
594,881 + Pf1N1B4_502 0.52 -0.9
594,882 - Pf1N1B4_502 0.52 -0.7
594,882 - Pf1N1B4_502 0.52 -2.3
594,968 + Pf1N1B4_502 0.58 -2.0
594,968 + Pf1N1B4_502 0.58 +1.3
594,968 + Pf1N1B4_502 0.58 +0.1
594,969 - Pf1N1B4_502 0.58 -0.6
594,969 - Pf1N1B4_502 0.58 +0.3
594,969 - Pf1N1B4_502 0.58 -0.3
594,969 - Pf1N1B4_502 0.58 -2.2
594,995 + Pf1N1B4_502 0.60 +1.0
594,996 - Pf1N1B4_502 0.60 -0.9
594,996 - Pf1N1B4_502 0.60 -0.4
594,996 - Pf1N1B4_502 0.60 -0.3
595,060 + Pf1N1B4_502 0.65 -1.5
595,142 + Pf1N1B4_502 0.71 +0.3
595,143 - Pf1N1B4_502 0.71 +0.5
595,146 + Pf1N1B4_502 0.72 -0.5
595,147 - Pf1N1B4_502 0.72 -0.3
595,147 - Pf1N1B4_502 0.72 -0.5
595,175 + +1.9
595,175 + Pf1N1B4_502 0.74 -0.5
595,176 - Pf1N1B4_502 0.74 -2.1
595,277 - Pf1N1B4_502 0.81 -0.1
595,277 - Pf1N1B4_502 0.81 -0.3
595,280 - +1.4
595,280 - Pf1N1B4_502 0.82 +1.7
595,496 + -1.5
595,496 + +2.5
595,496 + -2.2
595,497 - -0.3
595,507 - +0.7
595,529 - -2.2
595,561 + +1.1
595,561 + -0.0
595,643 + -0.2
595,645 + Pf1N1B4_503 0.22 -2.2
595,645 + Pf1N1B4_503 0.22 +1.3
595,645 + Pf1N1B4_503 0.22 +0.3
595,645 + Pf1N1B4_503 0.22 +0.8
595,645 + Pf1N1B4_503 0.22 -2.3
595,645 + Pf1N1B4_503 0.22 +0.8
595,783 - Pf1N1B4_503 0.48 -1.5
595,843 + Pf1N1B4_503 0.60 -1.8
595,843 + Pf1N1B4_503 0.60 -0.1
595,843 + Pf1N1B4_503 0.60 -0.9
595,843 + Pf1N1B4_503 0.60 -0.8
595,843 + Pf1N1B4_503 0.60 +0.1
595,843 + Pf1N1B4_503 0.60 -0.5
595,843 + Pf1N1B4_503 0.60 -2.7
595,843 + Pf1N1B4_503 0.60 +1.1
595,843 + Pf1N1B4_503 0.60 -1.1
595,843 + Pf1N1B4_503 0.60 -0.7
595,843 + Pf1N1B4_503 0.60 -1.8
595,843 + Pf1N1B4_503 0.60 -0.4
595,843 + Pf1N1B4_503 0.60 +0.4
595,843 + Pf1N1B4_503 0.60 +0.9
595,843 + -0.2
595,844 - Pf1N1B4_503 0.60 -0.7
595,844 - Pf1N1B4_503 0.60 +0.4
595,844 - Pf1N1B4_503 0.60 +0.8
595,844 - Pf1N1B4_503 0.60 -1.7
595,844 - Pf1N1B4_503 0.60 -2.4
595,844 - Pf1N1B4_503 0.60 -2.7
595,844 - Pf1N1B4_503 0.60 -0.4
595,884 + Pf1N1B4_503 0.67 -1.9
595,885 - Pf1N1B4_503 0.67 -1.1
595,918 + Pf1N1B4_503 0.74 -1.9
595,918 + Pf1N1B4_503 0.74 -1.4
595,918 + Pf1N1B4_503 0.74 -2.1
595,918 + Pf1N1B4_503 0.74 +0.6
595,919 - Pf1N1B4_503 0.74 -1.2
595,950 + Pf1N1B4_503 0.80 +0.1
596,034 - -1.5
596,071 - -0.4
596,071 - +1.7
596,071 - -0.9
596,074 - +1.3
596,074 - +1.6
596,082 + -0.8
596,082 + -0.2
596,083 - -2.7
596,083 - -0.5
596,085 + -0.3
596,086 - -1.1
596,092 + +1.5
596,092 + -1.8
596,093 - -1.9
596,202 + Pf1N1B4_504 0.61 -0.6
596,258 - -0.3
596,258 - -1.4
596,344 + -0.1
596,344 + -0.2
596,344 + +1.7
596,345 - -2.0
596,389 + -1.6
596,389 + -0.7
596,390 - +0.2
596,417 - +0.5
596,551 - -3.1
596,641 + -0.4
596,641 + -1.5
596,641 + -2.0
596,641 + +0.4
596,641 + +1.4
596,642 - -2.6
596,642 - +0.3
596,644 + -0.1
596,644 + +1.8
596,645 - +0.2
596,678 + -1.7
596,678 + -1.8
596,988 + -1.8
596,988 + +1.3
596,988 + +1.5
596,989 - -0.1
596,989 - -2.3
596,989 - -0.9

Or see this region's nucleotide sequence