Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4889 and Pf1N1B4_4890 overlap by 4 nucleotides Pf1N1B4_4890 and Pf1N1B4_4891 are separated by 122 nucleotides Pf1N1B4_4891 and Pf1N1B4_4892 are separated by 277 nucleotides
Pf1N1B4_4889 - Cystathionine beta-synthase (EC 4.2.1.22), at 5,411,076 to 5,412,452
_4889
Pf1N1B4_4890 - Cystathionine gamma-lyase (EC 4.4.1.1), at 5,412,449 to 5,413,630
_4890
Pf1N1B4_4891 - FIG00959288: hypothetical protein, at 5,413,753 to 5,414,028
_4891
Pf1N1B4_4892 - Transcriptional regulator, AraC family, at 5,414,306 to 5,415,121
_4892
Position (kb) 5412
5413
5414 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5411.457 kb on + strand, within Pf1N1B4_4889 at 5411.458 kb on - strand, within Pf1N1B4_4889 at 5411.461 kb on - strand, within Pf1N1B4_4889 at 5411.558 kb on + strand, within Pf1N1B4_4889 at 5411.665 kb on + strand, within Pf1N1B4_4889 at 5411.665 kb on + strand, within Pf1N1B4_4889 at 5411.666 kb on - strand, within Pf1N1B4_4889 at 5411.721 kb on + strand, within Pf1N1B4_4889 at 5411.721 kb on + strand, within Pf1N1B4_4889 at 5411.909 kb on + strand, within Pf1N1B4_4889 at 5411.909 kb on + strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.013 kb on - strand, within Pf1N1B4_4889 at 5412.015 kb on - strand, within Pf1N1B4_4889 at 5412.407 kb on + strand at 5412.569 kb on + strand, within Pf1N1B4_4890 at 5412.569 kb on + strand, within Pf1N1B4_4890 at 5412.570 kb on - strand, within Pf1N1B4_4890 at 5412.955 kb on + strand, within Pf1N1B4_4890 at 5412.955 kb on + strand, within Pf1N1B4_4890 at 5413.067 kb on + strand at 5413.067 kb on + strand at 5413.067 kb on + strand, within Pf1N1B4_4890 at 5413.067 kb on + strand, within Pf1N1B4_4890 at 5413.067 kb on + strand, within Pf1N1B4_4890 at 5413.068 kb on - strand, within Pf1N1B4_4890 at 5413.068 kb on - strand, within Pf1N1B4_4890 at 5413.302 kb on - strand, within Pf1N1B4_4890 at 5413.761 kb on - strand at 5414.306 kb on - strand at 5414.432 kb on + strand at 5414.432 kb on + strand, within Pf1N1B4_4892 at 5414.483 kb on + strand, within Pf1N1B4_4892 at 5414.483 kb on + strand, within Pf1N1B4_4892 at 5414.540 kb on - strand, within Pf1N1B4_4892
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 5,411,457 + Pf1N1B4_4889 0.28 -1.4 5,411,458 - Pf1N1B4_4889 0.28 -0.1 5,411,461 - Pf1N1B4_4889 0.28 -0.6 5,411,558 + Pf1N1B4_4889 0.35 +0.3 5,411,665 + Pf1N1B4_4889 0.43 -1.6 5,411,665 + Pf1N1B4_4889 0.43 -0.2 5,411,666 - Pf1N1B4_4889 0.43 -0.2 5,411,721 + Pf1N1B4_4889 0.47 +0.4 5,411,721 + Pf1N1B4_4889 0.47 -1.4 5,411,909 + Pf1N1B4_4889 0.60 +0.6 5,411,909 + Pf1N1B4_4889 0.60 +1.2 5,412,013 - Pf1N1B4_4889 0.68 +0.2 5,412,013 - Pf1N1B4_4889 0.68 -0.8 5,412,013 - Pf1N1B4_4889 0.68 -1.6 5,412,013 - Pf1N1B4_4889 0.68 -1.3 5,412,015 - Pf1N1B4_4889 0.68 -2.0 5,412,407 + +0.4 5,412,569 + Pf1N1B4_4890 0.10 +0.4 5,412,569 + Pf1N1B4_4890 0.10 +0.9 5,412,570 - Pf1N1B4_4890 0.10 +0.7 5,412,955 + Pf1N1B4_4890 0.43 -1.5 5,412,955 + Pf1N1B4_4890 0.43 -0.2 5,413,067 + -0.9 5,413,067 + +1.7 5,413,067 + Pf1N1B4_4890 0.52 +0.1 5,413,067 + Pf1N1B4_4890 0.52 -0.6 5,413,067 + Pf1N1B4_4890 0.52 -2.4 5,413,068 - Pf1N1B4_4890 0.52 -0.0 5,413,068 - Pf1N1B4_4890 0.52 +1.1 5,413,302 - Pf1N1B4_4890 0.72 -0.5 5,413,761 - -0.0 5,414,306 - -0.8 5,414,432 + +3.5 5,414,432 + Pf1N1B4_4892 0.15 +1.5 5,414,483 + Pf1N1B4_4892 0.22 -0.9 5,414,483 + Pf1N1B4_4892 0.22 -2.6 5,414,540 - Pf1N1B4_4892 0.29 +1.0
Or see this region's nucleotide sequence