Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4841

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4839 and Pf1N1B4_4840 are separated by 1 nucleotidesPf1N1B4_4840 and Pf1N1B4_4841 are separated by 685 nucleotidesPf1N1B4_4841 and Pf1N1B4_4842 overlap by 4 nucleotides Pf1N1B4_4839 - lipoprotein, at 5,345,845 to 5,346,639 _4839 Pf1N1B4_4840 - hypothetical protein, at 5,346,641 to 5,346,766 _4840 Pf1N1B4_4841 - hypothetical protein, at 5,347,452 to 5,348,303 _4841 Pf1N1B4_4842 - VgrG protein, at 5,348,300 to 5,350,372 _4842 Position (kb) 5347 5348 5349Strain fitness (log2 ratio) -3 -2 -1 0 1at 5346.485 kb on + strand, within Pf1N1B4_4839at 5346.486 kb on - strandat 5346.544 kb on - strand, within Pf1N1B4_4839at 5346.812 kb on - strandat 5346.852 kb on - strandat 5347.080 kb on - strandat 5347.080 kb on - strandat 5347.101 kb on + strandat 5347.264 kb on + strandat 5347.264 kb on + strandat 5347.290 kb on + strandat 5347.291 kb on - strandat 5347.291 kb on - strandat 5347.474 kb on - strandat 5347.752 kb on - strandat 5347.804 kb on + strand, within Pf1N1B4_4841at 5347.878 kb on + strandat 5348.197 kb on + strand, within Pf1N1B4_4841at 5348.632 kb on - strandat 5348.874 kb on + strand, within Pf1N1B4_4842at 5348.875 kb on - strand, within Pf1N1B4_4842at 5348.919 kb on + strand, within Pf1N1B4_4842at 5349.009 kb on + strand, within Pf1N1B4_4842

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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5,346,485 + Pf1N1B4_4839 0.81 -1.4
5,346,486 - -0.9
5,346,544 - Pf1N1B4_4839 0.88 +0.7
5,346,812 - -0.6
5,346,852 - -1.0
5,347,080 - +0.1
5,347,080 - +0.9
5,347,101 + +0.7
5,347,264 + -1.3
5,347,264 + -2.2
5,347,290 + -1.8
5,347,291 - +0.1
5,347,291 - +0.9
5,347,474 - -3.0
5,347,752 - -0.9
5,347,804 + Pf1N1B4_4841 0.41 -0.6
5,347,878 + -0.9
5,348,197 + Pf1N1B4_4841 0.87 -1.2
5,348,632 - -1.3
5,348,874 + Pf1N1B4_4842 0.28 -2.2
5,348,875 - Pf1N1B4_4842 0.28 -1.5
5,348,919 + Pf1N1B4_4842 0.30 -0.2
5,349,009 + Pf1N1B4_4842 0.34 -1.9

Or see this region's nucleotide sequence