Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4825 and Pf1N1B4_4826 are separated by 157 nucleotides Pf1N1B4_4826 and Pf1N1B4_4827 are separated by 164 nucleotides
Pf1N1B4_4825 - Putrescine ABC transporter putrescine-binding protein PotF (TC 3.A.1.11.2), at 5,329,241 to 5,330,329
_4825
Pf1N1B4_4826 - glutamine synthetase family protein, at 5,330,487 to 5,331,845
_4826
Pf1N1B4_4827 - Ethanolamine operon regulatory protein, at 5,332,010 to 5,332,936
_4827
Position (kb) 5330
5331
5332 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 5329.686 kb on + strand, within Pf1N1B4_4825 at 5329.695 kb on + strand, within Pf1N1B4_4825 at 5329.696 kb on - strand, within Pf1N1B4_4825 at 5329.696 kb on - strand, within Pf1N1B4_4825 at 5329.732 kb on + strand, within Pf1N1B4_4825 at 5329.773 kb on + strand at 5330.172 kb on + strand, within Pf1N1B4_4825 at 5330.173 kb on - strand, within Pf1N1B4_4825 at 5330.229 kb on + strand at 5330.245 kb on - strand at 5330.277 kb on - strand at 5330.321 kb on + strand at 5330.342 kb on + strand at 5330.342 kb on + strand at 5330.343 kb on - strand at 5330.348 kb on + strand at 5330.348 kb on + strand at 5330.349 kb on - strand at 5330.957 kb on - strand, within Pf1N1B4_4826 at 5331.640 kb on + strand, within Pf1N1B4_4826 at 5331.643 kb on + strand, within Pf1N1B4_4826 at 5331.643 kb on + strand, within Pf1N1B4_4826 at 5331.643 kb on + strand at 5331.643 kb on + strand, within Pf1N1B4_4826 at 5331.644 kb on - strand, within Pf1N1B4_4826 at 5331.858 kb on - strand at 5331.958 kb on + strand at 5331.959 kb on - strand at 5331.959 kb on - strand at 5331.967 kb on + strand at 5332.170 kb on - strand at 5332.262 kb on + strand at 5332.523 kb on + strand at 5332.523 kb on + strand, within Pf1N1B4_4827 at 5332.524 kb on - strand, within Pf1N1B4_4827 at 5332.524 kb on - strand, within Pf1N1B4_4827 at 5332.709 kb on + strand, within Pf1N1B4_4827 at 5332.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 5,329,686 + Pf1N1B4_4825 0.41 +0.8 5,329,695 + Pf1N1B4_4825 0.42 +0.4 5,329,696 - Pf1N1B4_4825 0.42 -2.7 5,329,696 - Pf1N1B4_4825 0.42 -0.2 5,329,732 + Pf1N1B4_4825 0.45 -0.5 5,329,773 + -0.5 5,330,172 + Pf1N1B4_4825 0.85 +1.0 5,330,173 - Pf1N1B4_4825 0.86 +0.1 5,330,229 + -0.3 5,330,245 - +1.0 5,330,277 - -1.3 5,330,321 + +0.8 5,330,342 + +1.6 5,330,342 + -0.1 5,330,343 - -0.2 5,330,348 + +2.4 5,330,348 + -0.1 5,330,349 - +3.1 5,330,957 - Pf1N1B4_4826 0.35 +1.1 5,331,640 + Pf1N1B4_4826 0.85 +1.6 5,331,643 + Pf1N1B4_4826 0.85 -0.3 5,331,643 + Pf1N1B4_4826 0.85 +3.1 5,331,643 + +0.1 5,331,643 + Pf1N1B4_4826 0.85 -0.9 5,331,644 - Pf1N1B4_4826 0.85 +0.2 5,331,858 - -0.9 5,331,958 + -0.0 5,331,959 - +3.3 5,331,959 - -0.1 5,331,967 + +0.7 5,332,170 - -0.3 5,332,262 + +1.4 5,332,523 + +0.1 5,332,523 + Pf1N1B4_4827 0.55 -0.6 5,332,524 - Pf1N1B4_4827 0.55 +1.0 5,332,524 - Pf1N1B4_4827 0.55 +0.3 5,332,709 + Pf1N1B4_4827 0.75 -1.1 5,332,710 - -0.6
Or see this region's nucleotide sequence