Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4636

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4634 and Pf1N1B4_4635 overlap by 4 nucleotidesPf1N1B4_4635 and Pf1N1B4_4636 overlap by 4 nucleotidesPf1N1B4_4636 and Pf1N1B4_4637 overlap by 4 nucleotides Pf1N1B4_4634 - BatD, at 5,110,955 to 5,112,604 _4634 Pf1N1B4_4635 - TPR domain protein in aerotolerance operon, at 5,112,601 to 5,112,732 _4635 Pf1N1B4_4636 - TPR domain protein in aerotolerance operon, at 5,112,729 to 5,114,336 _4636 Pf1N1B4_4637 - BatA (Bacteroides aerotolerance operon), at 5,114,333 to 5,115,427 _4637 Position (kb) 5112 5113 5114 5115Strain fitness (log2 ratio) -1 0 1 2 3at 5112.036 kb on + strand, within Pf1N1B4_4634at 5112.126 kb on + strand, within Pf1N1B4_4634at 5112.127 kb on - strandat 5112.127 kb on - strand, within Pf1N1B4_4634at 5112.254 kb on - strand, within Pf1N1B4_4634at 5113.060 kb on + strand, within Pf1N1B4_4636at 5113.117 kb on + strand, within Pf1N1B4_4636at 5113.183 kb on + strand, within Pf1N1B4_4636at 5113.309 kb on + strand, within Pf1N1B4_4636at 5113.309 kb on + strand, within Pf1N1B4_4636at 5114.363 kb on - strandat 5114.517 kb on + strandat 5114.991 kb on + strand, within Pf1N1B4_4637

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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5,112,036 + Pf1N1B4_4634 0.66 -0.6
5,112,126 + Pf1N1B4_4634 0.71 +2.0
5,112,127 - -0.8
5,112,127 - Pf1N1B4_4634 0.71 +0.3
5,112,254 - Pf1N1B4_4634 0.79 +1.9
5,113,060 + Pf1N1B4_4636 0.21 +1.2
5,113,117 + Pf1N1B4_4636 0.24 +0.5
5,113,183 + Pf1N1B4_4636 0.28 +1.4
5,113,309 + Pf1N1B4_4636 0.36 +1.0
5,113,309 + Pf1N1B4_4636 0.36 +2.6
5,114,363 - +0.7
5,114,517 + +3.5
5,114,991 + Pf1N1B4_4637 0.60 +1.5

Or see this region's nucleotide sequence