Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4633 and Pf1N1B4_4634 are separated by 248 nucleotides Pf1N1B4_4634 and Pf1N1B4_4635 overlap by 4 nucleotides Pf1N1B4_4635 and Pf1N1B4_4636 overlap by 4 nucleotides
Pf1N1B4_4633 - Exonuclease SbcD, at 5,109,450 to 5,110,706
_4633
Pf1N1B4_4634 - BatD, at 5,110,955 to 5,112,604
_4634
Pf1N1B4_4635 - TPR domain protein in aerotolerance operon, at 5,112,601 to 5,112,732
_4635
Pf1N1B4_4636 - TPR domain protein in aerotolerance operon, at 5,112,729 to 5,114,336
_4636
Position (kb) 5110
5111
5112
5113 Strain fitness (log2 ratio) -1
0
1
2 at 5110.028 kb on - strand, within Pf1N1B4_4633 at 5110.222 kb on + strand, within Pf1N1B4_4633 at 5110.222 kb on + strand, within Pf1N1B4_4633 at 5110.223 kb on - strand, within Pf1N1B4_4633 at 5110.227 kb on - strand, within Pf1N1B4_4633 at 5110.699 kb on + strand at 5110.700 kb on - strand at 5110.714 kb on + strand at 5110.715 kb on - strand at 5110.764 kb on - strand at 5110.872 kb on + strand at 5110.957 kb on - strand at 5111.079 kb on + strand at 5112.036 kb on + strand, within Pf1N1B4_4634 at 5112.126 kb on + strand, within Pf1N1B4_4634 at 5112.127 kb on - strand at 5112.127 kb on - strand, within Pf1N1B4_4634 at 5112.254 kb on - strand, within Pf1N1B4_4634 at 5113.060 kb on + strand, within Pf1N1B4_4636 at 5113.117 kb on + strand, within Pf1N1B4_4636 at 5113.183 kb on + strand, within Pf1N1B4_4636 at 5113.309 kb on + strand, within Pf1N1B4_4636 at 5113.309 kb on + strand, within Pf1N1B4_4636
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 5,110,028 - Pf1N1B4_4633 0.46 +2.0 5,110,222 + Pf1N1B4_4633 0.61 +1.3 5,110,222 + Pf1N1B4_4633 0.61 +1.5 5,110,223 - Pf1N1B4_4633 0.61 -0.9 5,110,227 - Pf1N1B4_4633 0.62 +1.6 5,110,699 + -0.5 5,110,700 - -0.2 5,110,714 + +0.5 5,110,715 - -0.5 5,110,764 - +0.1 5,110,872 + +0.8 5,110,957 - -0.3 5,111,079 + +2.3 5,112,036 + Pf1N1B4_4634 0.66 -0.6 5,112,126 + Pf1N1B4_4634 0.71 +2.0 5,112,127 - -0.8 5,112,127 - Pf1N1B4_4634 0.71 +0.3 5,112,254 - Pf1N1B4_4634 0.79 +1.9 5,113,060 + Pf1N1B4_4636 0.21 +1.2 5,113,117 + Pf1N1B4_4636 0.24 +0.5 5,113,183 + Pf1N1B4_4636 0.28 +1.4 5,113,309 + Pf1N1B4_4636 0.36 +1.0 5,113,309 + Pf1N1B4_4636 0.36 +2.6
Or see this region's nucleotide sequence