Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4592 and Pf1N1B4_4593 are separated by 4 nucleotides Pf1N1B4_4593 and Pf1N1B4_4594 overlap by 4 nucleotides
Pf1N1B4_4592 - Oligopeptide transport system permease protein OppB (TC 3.A.1.5.1), at 5,062,923 to 5,063,996
_4592
Pf1N1B4_4593 - ABC transporter, periplasmic substrate-binding protein, at 5,064,001 to 5,065,842
_4593
Pf1N1B4_4594 - Oligopeptide ABC transporter, periplasmic oligopeptide-binding protein OppA (TC 3.A.1.5.1), at 5,065,839 to 5,067,671
_4594
Position (kb)
5064
5065
5066 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 5063.249 kb on - strand, within Pf1N1B4_4592 at 5063.354 kb on - strand, within Pf1N1B4_4592 at 5063.746 kb on + strand, within Pf1N1B4_4592 at 5063.747 kb on - strand, within Pf1N1B4_4592 at 5064.098 kb on + strand at 5064.099 kb on - strand at 5064.099 kb on - strand at 5064.179 kb on + strand at 5064.183 kb on - strand at 5064.614 kb on + strand, within Pf1N1B4_4593 at 5064.983 kb on + strand, within Pf1N1B4_4593 at 5064.984 kb on - strand, within Pf1N1B4_4593 at 5064.984 kb on - strand, within Pf1N1B4_4593 at 5064.984 kb on - strand, within Pf1N1B4_4593 at 5065.448 kb on - strand, within Pf1N1B4_4593 at 5065.487 kb on + strand, within Pf1N1B4_4593 at 5065.488 kb on - strand, within Pf1N1B4_4593 at 5065.589 kb on + strand, within Pf1N1B4_4593 at 5065.703 kb on - strand at 5065.942 kb on + strand at 5065.942 kb on + strand at 5065.942 kb on + strand at 5065.969 kb on + strand at 5065.970 kb on - strand at 5065.970 kb on - strand at 5066.218 kb on + strand, within Pf1N1B4_4594 at 5066.570 kb on - strand at 5066.588 kb on - strand, within Pf1N1B4_4594 at 5066.633 kb on + strand, within Pf1N1B4_4594
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 5,063,249 - Pf1N1B4_4592 0.30 -2.6 5,063,354 - Pf1N1B4_4592 0.40 -0.3 5,063,746 + Pf1N1B4_4592 0.77 +0.5 5,063,747 - Pf1N1B4_4592 0.77 -2.9 5,064,098 + -1.0 5,064,099 - +0.4 5,064,099 - -1.5 5,064,179 + +0.2 5,064,183 - +0.2 5,064,614 + Pf1N1B4_4593 0.33 -1.5 5,064,983 + Pf1N1B4_4593 0.53 -0.8 5,064,984 - Pf1N1B4_4593 0.53 +0.2 5,064,984 - Pf1N1B4_4593 0.53 +0.2 5,064,984 - Pf1N1B4_4593 0.53 -2.3 5,065,448 - Pf1N1B4_4593 0.79 -0.3 5,065,487 + Pf1N1B4_4593 0.81 -3.1 5,065,488 - Pf1N1B4_4593 0.81 -1.1 5,065,589 + Pf1N1B4_4593 0.86 -2.0 5,065,703 - -1.9 5,065,942 + -0.9 5,065,942 + -1.7 5,065,942 + -1.3 5,065,969 + -1.9 5,065,970 - -0.2 5,065,970 - +1.8 5,066,218 + Pf1N1B4_4594 0.21 -2.8 5,066,570 - +0.5 5,066,588 - Pf1N1B4_4594 0.41 -1.4 5,066,633 + Pf1N1B4_4594 0.43 +1.3
Or see this region's nucleotide sequence