Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4509 and Pf1N1B4_4510 are separated by 16 nucleotides Pf1N1B4_4510 and Pf1N1B4_4511 are separated by 45 nucleotides Pf1N1B4_4511 and Pf1N1B4_4512 are separated by 281 nucleotides
Pf1N1B4_4509 - Aldose 1-epimerase, at 4,971,656 to 4,972,417
_4509
Pf1N1B4_4510 - Gluconolactonase (EC 3.1.1.17), at 4,972,434 to 4,973,327
_4510
Pf1N1B4_4511 - D-galacturonate dehydrogenase (EC 1.1.1.203) (from data) , at 4,973,373 to 4,974,185
_4511
Pf1N1B4_4512 - PhaK-like protein, at 4,974,467 to 4,975,654
_4512
Position (kb)
4973
4974
4975 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4972.834 kb on + strand, within Pf1N1B4_4510 at 4972.834 kb on + strand, within Pf1N1B4_4510 at 4972.834 kb on + strand at 4973.249 kb on - strand at 4973.352 kb on + strand at 4973.417 kb on - strand at 4973.560 kb on + strand, within Pf1N1B4_4511 at 4973.561 kb on - strand, within Pf1N1B4_4511 at 4973.590 kb on + strand, within Pf1N1B4_4511 at 4973.723 kb on - strand, within Pf1N1B4_4511 at 4973.767 kb on + strand, within Pf1N1B4_4511 at 4973.767 kb on + strand, within Pf1N1B4_4511 at 4973.887 kb on + strand, within Pf1N1B4_4511 at 4973.888 kb on - strand, within Pf1N1B4_4511 at 4974.097 kb on + strand, within Pf1N1B4_4511 at 4974.187 kb on + strand at 4974.187 kb on + strand at 4974.187 kb on + strand at 4974.187 kb on + strand at 4974.200 kb on + strand at 4974.200 kb on + strand at 4974.204 kb on + strand at 4974.204 kb on + strand at 4974.205 kb on - strand at 4974.205 kb on - strand at 4974.210 kb on + strand at 4974.210 kb on + strand at 4974.210 kb on + strand at 4974.210 kb on + strand at 4974.211 kb on - strand at 4974.253 kb on - strand at 4974.258 kb on + strand at 4974.258 kb on + strand at 4974.388 kb on - strand at 4974.440 kb on + strand at 4974.440 kb on + strand at 4974.493 kb on + strand at 4974.774 kb on + strand, within Pf1N1B4_4512 at 4974.774 kb on + strand, within Pf1N1B4_4512 at 4974.858 kb on + strand, within Pf1N1B4_4512 at 4974.859 kb on - strand, within Pf1N1B4_4512 at 4974.859 kb on - strand at 4974.876 kb on + strand, within Pf1N1B4_4512 at 4974.958 kb on - strand, within Pf1N1B4_4512 at 4975.017 kb on + strand, within Pf1N1B4_4512 at 4975.017 kb on + strand, within Pf1N1B4_4512 at 4975.017 kb on + strand, within Pf1N1B4_4512 at 4975.030 kb on - strand, within Pf1N1B4_4512 at 4975.089 kb on + strand, within Pf1N1B4_4512
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 4,972,834 + Pf1N1B4_4510 0.45 -0.4 4,972,834 + Pf1N1B4_4510 0.45 -0.4 4,972,834 + -1.2 4,973,249 - -1.2 4,973,352 + +0.3 4,973,417 - -2.1 4,973,560 + Pf1N1B4_4511 0.23 +0.4 4,973,561 - Pf1N1B4_4511 0.23 -2.8 4,973,590 + Pf1N1B4_4511 0.27 -1.0 4,973,723 - Pf1N1B4_4511 0.43 -1.7 4,973,767 + Pf1N1B4_4511 0.48 -0.9 4,973,767 + Pf1N1B4_4511 0.48 +1.6 4,973,887 + Pf1N1B4_4511 0.63 -1.8 4,973,888 - Pf1N1B4_4511 0.63 +0.2 4,974,097 + Pf1N1B4_4511 0.89 -0.3 4,974,187 + +2.8 4,974,187 + +2.2 4,974,187 + +2.1 4,974,187 + +0.3 4,974,200 + -0.5 4,974,200 + -0.0 4,974,204 + -0.2 4,974,204 + -1.2 4,974,205 - +1.3 4,974,205 - +0.3 4,974,210 + -0.7 4,974,210 + +1.1 4,974,210 + +0.2 4,974,210 + +0.4 4,974,211 - -0.0 4,974,253 - +1.4 4,974,258 + +0.8 4,974,258 + +0.5 4,974,388 - +1.1 4,974,440 + -0.4 4,974,440 + +0.1 4,974,493 + -1.5 4,974,774 + Pf1N1B4_4512 0.26 +0.3 4,974,774 + Pf1N1B4_4512 0.26 -1.0 4,974,858 + Pf1N1B4_4512 0.33 +0.5 4,974,859 - Pf1N1B4_4512 0.33 -2.0 4,974,859 - +0.6 4,974,876 + Pf1N1B4_4512 0.34 +0.6 4,974,958 - Pf1N1B4_4512 0.41 -0.8 4,975,017 + Pf1N1B4_4512 0.46 -1.7 4,975,017 + Pf1N1B4_4512 0.46 -0.7 4,975,017 + Pf1N1B4_4512 0.46 -0.7 4,975,030 - Pf1N1B4_4512 0.47 -1.7 4,975,089 + Pf1N1B4_4512 0.52 -2.1
Or see this region's nucleotide sequence