Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4214

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4213 and Pf1N1B4_4214 are separated by 106 nucleotidesPf1N1B4_4214 and Pf1N1B4_4215 are separated by 456 nucleotides Pf1N1B4_4213 - Potassium channel protein, at 4,666,941 to 4,667,816 _4213 Pf1N1B4_4214 - Arginine/ornithine antiporter ArcD, at 4,667,923 to 4,669,389 _4214 Pf1N1B4_4215 - Adenylate cyclase (EC 4.6.1.1), at 4,669,846 to 4,671,435 _4215 Position (kb) 4667 4668 4669 4670Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4667.049 kb on - strand, within Pf1N1B4_4213at 4667.049 kb on - strand, within Pf1N1B4_4213at 4667.094 kb on + strand, within Pf1N1B4_4213at 4667.094 kb on + strand, within Pf1N1B4_4213at 4667.188 kb on - strand, within Pf1N1B4_4213at 4667.188 kb on - strand, within Pf1N1B4_4213at 4667.194 kb on - strand, within Pf1N1B4_4213at 4667.197 kb on + strand, within Pf1N1B4_4213at 4667.198 kb on - strand, within Pf1N1B4_4213at 4667.198 kb on - strand, within Pf1N1B4_4213at 4667.249 kb on + strand, within Pf1N1B4_4213at 4667.249 kb on + strand, within Pf1N1B4_4213at 4667.251 kb on + strand, within Pf1N1B4_4213at 4667.252 kb on - strand, within Pf1N1B4_4213at 4667.252 kb on - strand, within Pf1N1B4_4213at 4667.252 kb on - strand, within Pf1N1B4_4213at 4667.252 kb on - strand, within Pf1N1B4_4213at 4667.252 kb on - strand, within Pf1N1B4_4213at 4667.261 kb on + strand, within Pf1N1B4_4213at 4667.433 kb on + strand, within Pf1N1B4_4213at 4667.434 kb on - strand, within Pf1N1B4_4213at 4667.436 kb on - strand, within Pf1N1B4_4213at 4667.475 kb on + strand, within Pf1N1B4_4213at 4667.499 kb on - strandat 4667.653 kb on - strand, within Pf1N1B4_4213at 4667.653 kb on - strand, within Pf1N1B4_4213at 4667.696 kb on + strand, within Pf1N1B4_4213at 4667.696 kb on + strand, within Pf1N1B4_4213at 4667.757 kb on + strandat 4667.757 kb on + strandat 4667.758 kb on - strandat 4667.945 kb on + strandat 4667.945 kb on + strandat 4667.974 kb on + strandat 4667.974 kb on + strandat 4667.975 kb on - strandat 4667.975 kb on - strandat 4667.975 kb on - strandat 4667.975 kb on - strandat 4667.975 kb on - strandat 4667.975 kb on - strandat 4667.975 kb on - strandat 4667.975 kb on - strandat 4667.975 kb on - strandat 4668.072 kb on - strand, within Pf1N1B4_4214at 4668.113 kb on + strand, within Pf1N1B4_4214at 4668.124 kb on + strand, within Pf1N1B4_4214at 4668.132 kb on - strandat 4668.191 kb on + strand, within Pf1N1B4_4214at 4668.192 kb on - strandat 4668.273 kb on - strand, within Pf1N1B4_4214at 4668.273 kb on - strand, within Pf1N1B4_4214at 4668.273 kb on - strand, within Pf1N1B4_4214at 4668.283 kb on + strand, within Pf1N1B4_4214at 4668.284 kb on - strand, within Pf1N1B4_4214at 4668.284 kb on - strand, within Pf1N1B4_4214at 4668.284 kb on - strand, within Pf1N1B4_4214at 4668.284 kb on - strand, within Pf1N1B4_4214at 4668.288 kb on - strand, within Pf1N1B4_4214at 4668.288 kb on - strand, within Pf1N1B4_4214at 4668.288 kb on - strand, within Pf1N1B4_4214at 4668.313 kb on - strand, within Pf1N1B4_4214at 4668.350 kb on - strand, within Pf1N1B4_4214at 4668.620 kb on - strand, within Pf1N1B4_4214at 4668.620 kb on - strand, within Pf1N1B4_4214at 4668.718 kb on - strandat 4668.891 kb on + strand, within Pf1N1B4_4214at 4669.127 kb on + strand, within Pf1N1B4_4214at 4669.128 kb on - strand, within Pf1N1B4_4214at 4669.249 kb on - strandat 4669.249 kb on - strandat 4669.332 kb on + strandat 4669.333 kb on - strandat 4669.684 kb on + strandat 4669.685 kb on - strandat 4669.697 kb on + strandat 4669.698 kb on - strandat 4669.698 kb on - strandat 4669.699 kb on + strandat 4670.185 kb on + strand, within Pf1N1B4_4215

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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4,667,049 - Pf1N1B4_4213 0.12 +1.4
4,667,049 - Pf1N1B4_4213 0.12 +1.5
4,667,094 + Pf1N1B4_4213 0.17 -0.8
4,667,094 + Pf1N1B4_4213 0.17 -2.2
4,667,188 - Pf1N1B4_4213 0.28 +0.6
4,667,188 - Pf1N1B4_4213 0.28 -2.0
4,667,194 - Pf1N1B4_4213 0.29 -1.7
4,667,197 + Pf1N1B4_4213 0.29 -0.6
4,667,198 - Pf1N1B4_4213 0.29 -1.3
4,667,198 - Pf1N1B4_4213 0.29 -1.6
4,667,249 + Pf1N1B4_4213 0.35 -0.0
4,667,249 + Pf1N1B4_4213 0.35 -0.1
4,667,251 + Pf1N1B4_4213 0.35 -1.5
4,667,252 - Pf1N1B4_4213 0.36 +0.4
4,667,252 - Pf1N1B4_4213 0.36 +2.2
4,667,252 - Pf1N1B4_4213 0.36 -2.2
4,667,252 - Pf1N1B4_4213 0.36 -1.8
4,667,252 - Pf1N1B4_4213 0.36 +0.6
4,667,261 + Pf1N1B4_4213 0.37 -0.1
4,667,433 + Pf1N1B4_4213 0.56 -3.0
4,667,434 - Pf1N1B4_4213 0.56 +0.3
4,667,436 - Pf1N1B4_4213 0.57 +0.4
4,667,475 + Pf1N1B4_4213 0.61 -1.3
4,667,499 - -0.3
4,667,653 - Pf1N1B4_4213 0.81 -0.3
4,667,653 - Pf1N1B4_4213 0.81 -2.9
4,667,696 + Pf1N1B4_4213 0.86 +1.0
4,667,696 + Pf1N1B4_4213 0.86 +0.9
4,667,757 + -0.0
4,667,757 + +0.3
4,667,758 - -2.1
4,667,945 + -1.0
4,667,945 + +0.1
4,667,974 + +2.3
4,667,974 + +0.4
4,667,975 - -2.3
4,667,975 - +0.1
4,667,975 - +0.6
4,667,975 - -0.8
4,667,975 - -1.1
4,667,975 - -0.4
4,667,975 - -2.6
4,667,975 - -0.1
4,667,975 - +0.5
4,668,072 - Pf1N1B4_4214 0.10 +0.3
4,668,113 + Pf1N1B4_4214 0.13 -0.3
4,668,124 + Pf1N1B4_4214 0.14 +0.9
4,668,132 - +1.7
4,668,191 + Pf1N1B4_4214 0.18 +0.6
4,668,192 - -0.1
4,668,273 - Pf1N1B4_4214 0.24 -1.3
4,668,273 - Pf1N1B4_4214 0.24 +1.6
4,668,273 - Pf1N1B4_4214 0.24 +0.1
4,668,283 + Pf1N1B4_4214 0.25 -1.2
4,668,284 - Pf1N1B4_4214 0.25 +0.8
4,668,284 - Pf1N1B4_4214 0.25 -0.3
4,668,284 - Pf1N1B4_4214 0.25 +1.9
4,668,284 - Pf1N1B4_4214 0.25 +2.3
4,668,288 - Pf1N1B4_4214 0.25 -0.7
4,668,288 - Pf1N1B4_4214 0.25 +0.8
4,668,288 - Pf1N1B4_4214 0.25 +0.3
4,668,313 - Pf1N1B4_4214 0.27 +0.6
4,668,350 - Pf1N1B4_4214 0.29 +0.3
4,668,620 - Pf1N1B4_4214 0.48 +0.6
4,668,620 - Pf1N1B4_4214 0.48 +0.4
4,668,718 - -1.1
4,668,891 + Pf1N1B4_4214 0.66 -0.7
4,669,127 + Pf1N1B4_4214 0.82 +1.4
4,669,128 - Pf1N1B4_4214 0.82 -0.1
4,669,249 - -0.4
4,669,249 - -0.7
4,669,332 + -1.6
4,669,333 - +1.2
4,669,684 + -0.1
4,669,685 - +0.7
4,669,697 + +0.5
4,669,698 - +2.6
4,669,698 - -1.3
4,669,699 + -0.4
4,670,185 + Pf1N1B4_4215 0.21 -0.9

Or see this region's nucleotide sequence