Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4213 and Pf1N1B4_4214 are separated by 106 nucleotides Pf1N1B4_4214 and Pf1N1B4_4215 are separated by 456 nucleotides
Pf1N1B4_4213 - Potassium channel protein, at 4,666,941 to 4,667,816
_4213
Pf1N1B4_4214 - Arginine/ornithine antiporter ArcD, at 4,667,923 to 4,669,389
_4214
Pf1N1B4_4215 - Adenylate cyclase (EC 4.6.1.1), at 4,669,846 to 4,671,435
_4215
Position (kb)
4667
4668
4669
4670 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4667.049 kb on - strand, within Pf1N1B4_4213 at 4667.049 kb on - strand, within Pf1N1B4_4213 at 4667.094 kb on + strand, within Pf1N1B4_4213 at 4667.094 kb on + strand, within Pf1N1B4_4213 at 4667.188 kb on - strand, within Pf1N1B4_4213 at 4667.188 kb on - strand, within Pf1N1B4_4213 at 4667.194 kb on - strand, within Pf1N1B4_4213 at 4667.197 kb on + strand, within Pf1N1B4_4213 at 4667.198 kb on - strand, within Pf1N1B4_4213 at 4667.198 kb on - strand, within Pf1N1B4_4213 at 4667.249 kb on + strand, within Pf1N1B4_4213 at 4667.249 kb on + strand, within Pf1N1B4_4213 at 4667.251 kb on + strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.252 kb on - strand, within Pf1N1B4_4213 at 4667.261 kb on + strand, within Pf1N1B4_4213 at 4667.433 kb on + strand, within Pf1N1B4_4213 at 4667.434 kb on - strand, within Pf1N1B4_4213 at 4667.436 kb on - strand, within Pf1N1B4_4213 at 4667.475 kb on + strand, within Pf1N1B4_4213 at 4667.499 kb on - strand at 4667.653 kb on - strand, within Pf1N1B4_4213 at 4667.653 kb on - strand, within Pf1N1B4_4213 at 4667.696 kb on + strand, within Pf1N1B4_4213 at 4667.696 kb on + strand, within Pf1N1B4_4213 at 4667.757 kb on + strand at 4667.757 kb on + strand at 4667.758 kb on - strand at 4667.945 kb on + strand at 4667.945 kb on + strand at 4667.974 kb on + strand at 4667.974 kb on + strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4667.975 kb on - strand at 4668.072 kb on - strand, within Pf1N1B4_4214 at 4668.113 kb on + strand, within Pf1N1B4_4214 at 4668.124 kb on + strand, within Pf1N1B4_4214 at 4668.132 kb on - strand at 4668.191 kb on + strand, within Pf1N1B4_4214 at 4668.192 kb on - strand at 4668.273 kb on - strand, within Pf1N1B4_4214 at 4668.273 kb on - strand, within Pf1N1B4_4214 at 4668.273 kb on - strand, within Pf1N1B4_4214 at 4668.283 kb on + strand, within Pf1N1B4_4214 at 4668.284 kb on - strand, within Pf1N1B4_4214 at 4668.284 kb on - strand, within Pf1N1B4_4214 at 4668.284 kb on - strand, within Pf1N1B4_4214 at 4668.284 kb on - strand, within Pf1N1B4_4214 at 4668.288 kb on - strand, within Pf1N1B4_4214 at 4668.288 kb on - strand, within Pf1N1B4_4214 at 4668.288 kb on - strand, within Pf1N1B4_4214 at 4668.313 kb on - strand, within Pf1N1B4_4214 at 4668.350 kb on - strand, within Pf1N1B4_4214 at 4668.620 kb on - strand, within Pf1N1B4_4214 at 4668.620 kb on - strand, within Pf1N1B4_4214 at 4668.718 kb on - strand at 4668.891 kb on + strand, within Pf1N1B4_4214 at 4669.127 kb on + strand, within Pf1N1B4_4214 at 4669.128 kb on - strand, within Pf1N1B4_4214 at 4669.249 kb on - strand at 4669.249 kb on - strand at 4669.332 kb on + strand at 4669.333 kb on - strand at 4669.684 kb on + strand at 4669.685 kb on - strand at 4669.697 kb on + strand at 4669.698 kb on - strand at 4669.698 kb on - strand at 4669.699 kb on + strand at 4670.185 kb on + strand, within Pf1N1B4_4215
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 4,667,049 - Pf1N1B4_4213 0.12 +1.4 4,667,049 - Pf1N1B4_4213 0.12 +1.5 4,667,094 + Pf1N1B4_4213 0.17 -0.8 4,667,094 + Pf1N1B4_4213 0.17 -2.2 4,667,188 - Pf1N1B4_4213 0.28 +0.6 4,667,188 - Pf1N1B4_4213 0.28 -2.0 4,667,194 - Pf1N1B4_4213 0.29 -1.7 4,667,197 + Pf1N1B4_4213 0.29 -0.6 4,667,198 - Pf1N1B4_4213 0.29 -1.3 4,667,198 - Pf1N1B4_4213 0.29 -1.6 4,667,249 + Pf1N1B4_4213 0.35 -0.0 4,667,249 + Pf1N1B4_4213 0.35 -0.1 4,667,251 + Pf1N1B4_4213 0.35 -1.5 4,667,252 - Pf1N1B4_4213 0.36 +0.4 4,667,252 - Pf1N1B4_4213 0.36 +2.2 4,667,252 - Pf1N1B4_4213 0.36 -2.2 4,667,252 - Pf1N1B4_4213 0.36 -1.8 4,667,252 - Pf1N1B4_4213 0.36 +0.6 4,667,261 + Pf1N1B4_4213 0.37 -0.1 4,667,433 + Pf1N1B4_4213 0.56 -3.0 4,667,434 - Pf1N1B4_4213 0.56 +0.3 4,667,436 - Pf1N1B4_4213 0.57 +0.4 4,667,475 + Pf1N1B4_4213 0.61 -1.3 4,667,499 - -0.3 4,667,653 - Pf1N1B4_4213 0.81 -0.3 4,667,653 - Pf1N1B4_4213 0.81 -2.9 4,667,696 + Pf1N1B4_4213 0.86 +1.0 4,667,696 + Pf1N1B4_4213 0.86 +0.9 4,667,757 + -0.0 4,667,757 + +0.3 4,667,758 - -2.1 4,667,945 + -1.0 4,667,945 + +0.1 4,667,974 + +2.3 4,667,974 + +0.4 4,667,975 - -2.3 4,667,975 - +0.1 4,667,975 - +0.6 4,667,975 - -0.8 4,667,975 - -1.1 4,667,975 - -0.4 4,667,975 - -2.6 4,667,975 - -0.1 4,667,975 - +0.5 4,668,072 - Pf1N1B4_4214 0.10 +0.3 4,668,113 + Pf1N1B4_4214 0.13 -0.3 4,668,124 + Pf1N1B4_4214 0.14 +0.9 4,668,132 - +1.7 4,668,191 + Pf1N1B4_4214 0.18 +0.6 4,668,192 - -0.1 4,668,273 - Pf1N1B4_4214 0.24 -1.3 4,668,273 - Pf1N1B4_4214 0.24 +1.6 4,668,273 - Pf1N1B4_4214 0.24 +0.1 4,668,283 + Pf1N1B4_4214 0.25 -1.2 4,668,284 - Pf1N1B4_4214 0.25 +0.8 4,668,284 - Pf1N1B4_4214 0.25 -0.3 4,668,284 - Pf1N1B4_4214 0.25 +1.9 4,668,284 - Pf1N1B4_4214 0.25 +2.3 4,668,288 - Pf1N1B4_4214 0.25 -0.7 4,668,288 - Pf1N1B4_4214 0.25 +0.8 4,668,288 - Pf1N1B4_4214 0.25 +0.3 4,668,313 - Pf1N1B4_4214 0.27 +0.6 4,668,350 - Pf1N1B4_4214 0.29 +0.3 4,668,620 - Pf1N1B4_4214 0.48 +0.6 4,668,620 - Pf1N1B4_4214 0.48 +0.4 4,668,718 - -1.1 4,668,891 + Pf1N1B4_4214 0.66 -0.7 4,669,127 + Pf1N1B4_4214 0.82 +1.4 4,669,128 - Pf1N1B4_4214 0.82 -0.1 4,669,249 - -0.4 4,669,249 - -0.7 4,669,332 + -1.6 4,669,333 - +1.2 4,669,684 + -0.1 4,669,685 - +0.7 4,669,697 + +0.5 4,669,698 - +2.6 4,669,698 - -1.3 4,669,699 + -0.4 4,670,185 + Pf1N1B4_4215 0.21 -0.9
Or see this region's nucleotide sequence