Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3748 and Pf1N1B4_3749 are separated by 350 nucleotides Pf1N1B4_3749 and Pf1N1B4_3750 are separated by 160 nucleotides
Pf1N1B4_3748 - ABC-type Fe3+ transport system, periplasmic component, at 4,159,294 to 4,160,358
_3748
Pf1N1B4_3749 - 2-aminoethylphosphonate uptake and metabolism regulator, at 4,160,709 to 4,161,422
_3749
Pf1N1B4_3750 - Potassium efflux system KefA protein / Small-conductance mechanosensitive channel, at 4,161,583 to 4,163,670
_3750
Position (kb)
4160
4161
4162 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4159.816 kb on + strand, within Pf1N1B4_3748 at 4159.869 kb on - strand, within Pf1N1B4_3748 at 4159.958 kb on + strand, within Pf1N1B4_3748 at 4159.958 kb on + strand, within Pf1N1B4_3748 at 4160.025 kb on + strand, within Pf1N1B4_3748 at 4160.025 kb on + strand, within Pf1N1B4_3748 at 4160.025 kb on + strand, within Pf1N1B4_3748 at 4160.025 kb on + strand, within Pf1N1B4_3748 at 4160.026 kb on - strand, within Pf1N1B4_3748 at 4160.026 kb on - strand, within Pf1N1B4_3748 at 4160.027 kb on + strand at 4160.028 kb on + strand, within Pf1N1B4_3748 at 4160.174 kb on + strand at 4160.175 kb on - strand, within Pf1N1B4_3748 at 4160.175 kb on - strand, within Pf1N1B4_3748 at 4160.420 kb on + strand at 4160.421 kb on - strand at 4160.421 kb on - strand at 4160.540 kb on + strand at 4160.540 kb on + strand at 4160.540 kb on + strand at 4160.613 kb on + strand at 4160.613 kb on + strand at 4160.614 kb on - strand at 4160.614 kb on - strand at 4160.614 kb on - strand at 4160.624 kb on + strand at 4160.625 kb on - strand at 4160.697 kb on + strand at 4160.863 kb on + strand, within Pf1N1B4_3749 at 4160.940 kb on + strand, within Pf1N1B4_3749 at 4160.940 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.141 kb on + strand, within Pf1N1B4_3749 at 4161.141 kb on + strand, within Pf1N1B4_3749 at 4161.207 kb on + strand, within Pf1N1B4_3749 at 4161.207 kb on + strand, within Pf1N1B4_3749 at 4161.208 kb on - strand, within Pf1N1B4_3749 at 4161.208 kb on - strand, within Pf1N1B4_3749 at 4161.208 kb on - strand, within Pf1N1B4_3749 at 4161.208 kb on - strand at 4161.339 kb on + strand, within Pf1N1B4_3749 at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.528 kb on + strand at 4161.529 kb on - strand at 4161.529 kb on - strand at 4161.529 kb on - strand at 4161.529 kb on - strand at 4161.573 kb on - strand at 4161.585 kb on - strand at 4161.585 kb on - strand at 4161.656 kb on - strand at 4162.031 kb on + strand, within Pf1N1B4_3750
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 4,159,816 + Pf1N1B4_3748 0.49 +0.9 4,159,869 - Pf1N1B4_3748 0.54 +0.7 4,159,958 + Pf1N1B4_3748 0.62 -1.1 4,159,958 + Pf1N1B4_3748 0.62 -0.4 4,160,025 + Pf1N1B4_3748 0.69 -1.0 4,160,025 + Pf1N1B4_3748 0.69 -0.1 4,160,025 + Pf1N1B4_3748 0.69 -0.7 4,160,025 + Pf1N1B4_3748 0.69 -1.4 4,160,026 - Pf1N1B4_3748 0.69 -1.0 4,160,026 - Pf1N1B4_3748 0.69 -1.2 4,160,027 + -1.2 4,160,028 + Pf1N1B4_3748 0.69 -1.2 4,160,174 + +0.8 4,160,175 - Pf1N1B4_3748 0.83 -1.7 4,160,175 - Pf1N1B4_3748 0.83 -1.0 4,160,420 + +0.2 4,160,421 - -0.3 4,160,421 - -1.1 4,160,540 + -0.8 4,160,540 + -0.1 4,160,540 + +1.1 4,160,613 + -2.5 4,160,613 + +1.2 4,160,614 - -1.9 4,160,614 - -1.3 4,160,614 - -1.6 4,160,624 + +1.6 4,160,625 - +2.0 4,160,697 + -1.8 4,160,863 + Pf1N1B4_3749 0.22 -0.2 4,160,940 + Pf1N1B4_3749 0.32 +1.0 4,160,940 + Pf1N1B4_3749 0.32 -0.3 4,161,129 + Pf1N1B4_3749 0.59 -0.7 4,161,129 + Pf1N1B4_3749 0.59 -2.2 4,161,129 + Pf1N1B4_3749 0.59 -0.3 4,161,129 + Pf1N1B4_3749 0.59 -1.5 4,161,129 + Pf1N1B4_3749 0.59 -2.7 4,161,129 + Pf1N1B4_3749 0.59 -2.3 4,161,129 + Pf1N1B4_3749 0.59 -0.7 4,161,129 + Pf1N1B4_3749 0.59 -0.5 4,161,130 - Pf1N1B4_3749 0.59 -1.7 4,161,130 - Pf1N1B4_3749 0.59 +3.6 4,161,130 - Pf1N1B4_3749 0.59 -1.3 4,161,130 - Pf1N1B4_3749 0.59 -0.4 4,161,130 - Pf1N1B4_3749 0.59 -1.0 4,161,141 + Pf1N1B4_3749 0.61 +0.1 4,161,141 + Pf1N1B4_3749 0.61 -1.2 4,161,207 + Pf1N1B4_3749 0.70 -0.7 4,161,207 + Pf1N1B4_3749 0.70 -2.0 4,161,208 - Pf1N1B4_3749 0.70 +1.1 4,161,208 - Pf1N1B4_3749 0.70 -1.0 4,161,208 - Pf1N1B4_3749 0.70 +0.4 4,161,208 - -1.6 4,161,339 + Pf1N1B4_3749 0.88 -0.6 4,161,528 + +0.9 4,161,528 + +0.1 4,161,528 + -2.0 4,161,528 + -0.0 4,161,528 + -1.7 4,161,528 + -1.2 4,161,528 + -0.3 4,161,528 + +0.4 4,161,528 + -0.8 4,161,529 - -0.5 4,161,529 - +0.1 4,161,529 - -0.8 4,161,529 - +2.5 4,161,573 - +0.6 4,161,585 - +0.8 4,161,585 - +0.4 4,161,656 - -3.3 4,162,031 + Pf1N1B4_3750 0.21 -1.1
Or see this region's nucleotide sequence