Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3645

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3644 and Pf1N1B4_3645 are separated by 11 nucleotidesPf1N1B4_3645 and Pf1N1B4_3646 overlap by 4 nucleotidesPf1N1B4_3646 and Pf1N1B4_3647 overlap by 8 nucleotides Pf1N1B4_3644 - Cytochrome c heme lyase subunit CcmF, at 4,049,334 to 4,051,322 _3644 Pf1N1B4_3645 - Cytochrome c-type biogenesis protein CcmG/DsbE, thiol:disulfide oxidoreductase, at 4,051,334 to 4,051,855 _3645 Pf1N1B4_3646 - Cytochrome c heme lyase subunit CcmL, at 4,051,852 to 4,052,325 _3646 Pf1N1B4_3647 - Cytochrome c heme lyase subunit CcmH, at 4,052,318 to 4,053,520 _3647 Position (kb) 4051 4052Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4051.063 kb on - strand, within Pf1N1B4_3644at 4051.575 kb on + strand, within Pf1N1B4_3645at 4051.823 kb on + strandat 4051.823 kb on + strandat 4052.308 kb on - strandat 4052.646 kb on - strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.777 kb on + strand, within Pf1N1B4_3647at 4052.778 kb on - strand, within Pf1N1B4_3647at 4052.778 kb on - strand, within Pf1N1B4_3647at 4052.778 kb on - strand, within Pf1N1B4_3647at 4052.778 kb on - strand, within Pf1N1B4_3647at 4052.778 kb on - strand, within Pf1N1B4_3647at 4052.778 kb on - strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.795 kb on + strand, within Pf1N1B4_3647at 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strandat 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strandat 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.796 kb on - strand, within Pf1N1B4_3647at 4052.847 kb on + strand, within Pf1N1B4_3647at 4052.847 kb on + strand, within Pf1N1B4_3647at 4052.847 kb on + strand, within Pf1N1B4_3647at 4052.847 kb on + strand, within Pf1N1B4_3647at 4052.848 kb on - strandat 4052.848 kb on - strand, within Pf1N1B4_3647at 4052.848 kb on - strand, within Pf1N1B4_3647

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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4,051,063 - Pf1N1B4_3644 0.87 +1.4
4,051,575 + Pf1N1B4_3645 0.46 +0.2
4,051,823 + +1.7
4,051,823 + -1.2
4,052,308 - -2.5
4,052,646 - Pf1N1B4_3647 0.27 -0.7
4,052,777 + Pf1N1B4_3647 0.38 +1.7
4,052,777 + Pf1N1B4_3647 0.38 +0.8
4,052,777 + Pf1N1B4_3647 0.38 +1.1
4,052,777 + Pf1N1B4_3647 0.38 +1.1
4,052,777 + Pf1N1B4_3647 0.38 +0.5
4,052,777 + Pf1N1B4_3647 0.38 -0.4
4,052,777 + Pf1N1B4_3647 0.38 +0.9
4,052,777 + Pf1N1B4_3647 0.38 +0.6
4,052,777 + Pf1N1B4_3647 0.38 +1.7
4,052,777 + Pf1N1B4_3647 0.38 +0.8
4,052,777 + Pf1N1B4_3647 0.38 +1.0
4,052,777 + Pf1N1B4_3647 0.38 +2.1
4,052,777 + Pf1N1B4_3647 0.38 +1.4
4,052,777 + Pf1N1B4_3647 0.38 -0.2
4,052,777 + Pf1N1B4_3647 0.38 -0.3
4,052,778 - Pf1N1B4_3647 0.38 +0.2
4,052,778 - Pf1N1B4_3647 0.38 -1.9
4,052,778 - Pf1N1B4_3647 0.38 +0.4
4,052,778 - Pf1N1B4_3647 0.38 +0.0
4,052,778 - Pf1N1B4_3647 0.38 -0.9
4,052,778 - Pf1N1B4_3647 0.38 +0.4
4,052,795 + Pf1N1B4_3647 0.40 +1.8
4,052,795 + Pf1N1B4_3647 0.40 +1.3
4,052,795 + Pf1N1B4_3647 0.40 -2.1
4,052,795 + Pf1N1B4_3647 0.40 +1.0
4,052,795 + Pf1N1B4_3647 0.40 +2.0
4,052,795 + Pf1N1B4_3647 0.40 +0.5
4,052,795 + Pf1N1B4_3647 0.40 +0.9
4,052,795 + Pf1N1B4_3647 0.40 +0.6
4,052,795 + Pf1N1B4_3647 0.40 +0.2
4,052,795 + Pf1N1B4_3647 0.40 +0.9
4,052,795 + Pf1N1B4_3647 0.40 -1.1
4,052,795 + Pf1N1B4_3647 0.40 -1.1
4,052,795 + Pf1N1B4_3647 0.40 +2.5
4,052,795 + Pf1N1B4_3647 0.40 -0.6
4,052,795 + Pf1N1B4_3647 0.40 +1.6
4,052,795 + Pf1N1B4_3647 0.40 +1.3
4,052,795 + Pf1N1B4_3647 0.40 -1.9
4,052,795 + Pf1N1B4_3647 0.40 -2.8
4,052,795 + Pf1N1B4_3647 0.40 +1.2
4,052,795 + Pf1N1B4_3647 0.40 +0.7
4,052,796 - Pf1N1B4_3647 0.40 +0.4
4,052,796 - Pf1N1B4_3647 0.40 +0.4
4,052,796 - Pf1N1B4_3647 0.40 -0.0
4,052,796 - Pf1N1B4_3647 0.40 +2.5
4,052,796 - +2.0
4,052,796 - Pf1N1B4_3647 0.40 -1.4
4,052,796 - -0.6
4,052,796 - Pf1N1B4_3647 0.40 +1.4
4,052,796 - Pf1N1B4_3647 0.40 +1.4
4,052,796 - Pf1N1B4_3647 0.40 +1.2
4,052,796 - Pf1N1B4_3647 0.40 +0.7
4,052,796 - Pf1N1B4_3647 0.40 -1.6
4,052,796 - Pf1N1B4_3647 0.40 +0.4
4,052,796 - Pf1N1B4_3647 0.40 +1.2
4,052,796 - Pf1N1B4_3647 0.40 -1.7
4,052,796 - Pf1N1B4_3647 0.40 +0.6
4,052,847 + Pf1N1B4_3647 0.44 +0.5
4,052,847 + Pf1N1B4_3647 0.44 -0.2
4,052,847 + Pf1N1B4_3647 0.44 -0.2
4,052,847 + Pf1N1B4_3647 0.44 +1.2
4,052,848 - -0.7
4,052,848 - Pf1N1B4_3647 0.44 +0.3
4,052,848 - Pf1N1B4_3647 0.44 +1.3

Or see this region's nucleotide sequence