Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3580 and Pf1N1B4_3581 overlap by 4 nucleotides Pf1N1B4_3581 and Pf1N1B4_3582 overlap by 11 nucleotides
Pf1N1B4_3580 - methyltransferase, putative, at 3,990,533 to 3,991,759
_3580
Pf1N1B4_3581 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 3,991,756 to 3,992,613
_3581
Pf1N1B4_3582 - C-methyltransferase, at 3,992,603 to 3,993,673
_3582
Position (kb)
3991
3992
3993 Strain fitness (log2 ratio)
-2
-1
0
1 at 3990.786 kb on - strand, within Pf1N1B4_3580 at 3990.873 kb on - strand, within Pf1N1B4_3580 at 3990.873 kb on - strand, within Pf1N1B4_3580 at 3991.190 kb on + strand, within Pf1N1B4_3580 at 3991.190 kb on + strand, within Pf1N1B4_3580 at 3991.190 kb on + strand, within Pf1N1B4_3580 at 3991.190 kb on + strand, within Pf1N1B4_3580 at 3991.191 kb on - strand, within Pf1N1B4_3580 at 3991.417 kb on + strand, within Pf1N1B4_3580 at 3991.417 kb on + strand, within Pf1N1B4_3580 at 3991.417 kb on + strand, within Pf1N1B4_3580 at 3991.418 kb on - strand, within Pf1N1B4_3580 at 3991.517 kb on + strand, within Pf1N1B4_3580 at 3991.583 kb on + strand, within Pf1N1B4_3580 at 3991.583 kb on + strand, within Pf1N1B4_3580 at 3991.583 kb on + strand, within Pf1N1B4_3580 at 3991.583 kb on + strand, within Pf1N1B4_3580 at 3991.584 kb on - strand, within Pf1N1B4_3580 at 3991.584 kb on - strand, within Pf1N1B4_3580 at 3991.646 kb on + strand at 3991.646 kb on + strand at 3991.647 kb on - strand at 3991.647 kb on - strand at 3991.647 kb on - strand at 3991.647 kb on - strand at 3991.647 kb on - strand at 3991.704 kb on + strand at 3991.705 kb on - strand at 3991.911 kb on + strand, within Pf1N1B4_3581 at 3991.996 kb on + strand, within Pf1N1B4_3581 at 3991.996 kb on + strand, within Pf1N1B4_3581 at 3991.997 kb on - strand, within Pf1N1B4_3581 at 3992.022 kb on + strand, within Pf1N1B4_3581 at 3992.022 kb on + strand, within Pf1N1B4_3581 at 3992.023 kb on - strand, within Pf1N1B4_3581 at 3992.251 kb on + strand, within Pf1N1B4_3581 at 3992.588 kb on + strand at 3992.589 kb on - strand at 3992.589 kb on - strand at 3992.589 kb on - strand at 3992.618 kb on + strand at 3992.768 kb on + strand, within Pf1N1B4_3582 at 3992.769 kb on - strand, within Pf1N1B4_3582 at 3992.780 kb on + strand, within Pf1N1B4_3582 at 3993.039 kb on + strand, within Pf1N1B4_3582 at 3993.263 kb on + strand, within Pf1N1B4_3582 at 3993.599 kb on + strand at 3993.599 kb on + strand at 3993.600 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 3,990,786 - Pf1N1B4_3580 0.21 +0.6 3,990,873 - Pf1N1B4_3580 0.28 +0.0 3,990,873 - Pf1N1B4_3580 0.28 -0.5 3,991,190 + Pf1N1B4_3580 0.54 -0.2 3,991,190 + Pf1N1B4_3580 0.54 -0.8 3,991,190 + Pf1N1B4_3580 0.54 +1.3 3,991,190 + Pf1N1B4_3580 0.54 -1.4 3,991,191 - Pf1N1B4_3580 0.54 +1.6 3,991,417 + Pf1N1B4_3580 0.72 -1.9 3,991,417 + Pf1N1B4_3580 0.72 +1.7 3,991,417 + Pf1N1B4_3580 0.72 +1.4 3,991,418 - Pf1N1B4_3580 0.72 +0.5 3,991,517 + Pf1N1B4_3580 0.80 +0.8 3,991,583 + Pf1N1B4_3580 0.86 +1.4 3,991,583 + Pf1N1B4_3580 0.86 -1.8 3,991,583 + Pf1N1B4_3580 0.86 +0.9 3,991,583 + Pf1N1B4_3580 0.86 -0.6 3,991,584 - Pf1N1B4_3580 0.86 +0.5 3,991,584 - Pf1N1B4_3580 0.86 -2.6 3,991,646 + +0.4 3,991,646 + +1.1 3,991,647 - -1.2 3,991,647 - -0.3 3,991,647 - +0.5 3,991,647 - +0.1 3,991,647 - -1.6 3,991,704 + +0.6 3,991,705 - -2.3 3,991,911 + Pf1N1B4_3581 0.18 +0.4 3,991,996 + Pf1N1B4_3581 0.28 -2.2 3,991,996 + Pf1N1B4_3581 0.28 +0.3 3,991,997 - Pf1N1B4_3581 0.28 -1.4 3,992,022 + Pf1N1B4_3581 0.31 +0.4 3,992,022 + Pf1N1B4_3581 0.31 -1.9 3,992,023 - Pf1N1B4_3581 0.31 +0.5 3,992,251 + Pf1N1B4_3581 0.58 +0.9 3,992,588 + -1.6 3,992,589 - +1.4 3,992,589 - -2.3 3,992,589 - +0.3 3,992,618 + -0.6 3,992,768 + Pf1N1B4_3582 0.15 -2.6 3,992,769 - Pf1N1B4_3582 0.15 +0.0 3,992,780 + Pf1N1B4_3582 0.17 +0.1 3,993,039 + Pf1N1B4_3582 0.41 -1.9 3,993,263 + Pf1N1B4_3582 0.62 -0.1 3,993,599 + +0.2 3,993,599 + -0.3 3,993,600 - -0.9
Or see this region's nucleotide sequence