Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3517 and Pf1N1B4_3518 are separated by 15 nucleotides Pf1N1B4_3518 and Pf1N1B4_3519 are separated by 31 nucleotides Pf1N1B4_3519 and Pf1N1B4_3520 overlap by 4 nucleotides Pf1N1B4_3520 and Pf1N1B4_3521 are separated by 194 nucleotides
Pf1N1B4_3517 - hypothetical protein, at 3,925,609 to 3,925,827
_3517
Pf1N1B4_3518 - Glycerol-3-phosphate dehydrogenase [NAD(P)+] (EC 1.1.1.94), at 3,925,843 to 3,926,769
_3518
Pf1N1B4_3519 - Lipase (EC 3.1.1.3), at 3,926,801 to 3,927,148
_3519
Pf1N1B4_3520 - Phosphohistidine phosphatase SixA, at 3,927,145 to 3,927,597
_3520
Pf1N1B4_3521 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 3,927,792 to 3,929,459
_3521
Position (kb)
3926
3927
3928 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3926.606 kb on + strand, within Pf1N1B4_3518 at 3926.607 kb on - strand, within Pf1N1B4_3518 at 3926.638 kb on + strand, within Pf1N1B4_3518 at 3926.638 kb on + strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.639 kb on - strand, within Pf1N1B4_3518 at 3926.708 kb on + strand at 3926.745 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.767 kb on + strand at 3926.768 kb on - strand at 3926.768 kb on - strand at 3926.771 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.781 kb on + strand at 3926.782 kb on - strand at 3926.782 kb on - strand at 3926.782 kb on - strand at 3926.782 kb on - strand at 3926.828 kb on + strand at 3926.828 kb on + strand at 3926.828 kb on + strand at 3926.828 kb on + strand at 3926.867 kb on + strand, within Pf1N1B4_3519 at 3926.867 kb on + strand, within Pf1N1B4_3519 at 3926.867 kb on + strand at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.919 kb on + strand, within Pf1N1B4_3519 at 3926.920 kb on - strand, within Pf1N1B4_3519 at 3926.931 kb on - strand, within Pf1N1B4_3519 at 3926.942 kb on + strand, within Pf1N1B4_3519 at 3927.144 kb on + strand at 3927.159 kb on - strand at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.286 kb on + strand, within Pf1N1B4_3520 at 3927.287 kb on - strand, within Pf1N1B4_3520 at 3927.419 kb on + strand, within Pf1N1B4_3520 at 3927.478 kb on + strand, within Pf1N1B4_3520 at 3927.479 kb on - strand, within Pf1N1B4_3520 at 3927.479 kb on - strand, within Pf1N1B4_3520 at 3927.479 kb on - strand, within Pf1N1B4_3520 at 3927.639 kb on + strand at 3927.640 kb on - strand at 3927.727 kb on - strand at 3927.828 kb on + strand at 3927.828 kb on + strand at 3927.828 kb on + strand at 3927.828 kb on + strand at 3927.829 kb on - strand at 3927.829 kb on - strand at 3927.829 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 3,926,606 + Pf1N1B4_3518 0.82 -2.1 3,926,607 - Pf1N1B4_3518 0.82 -0.6 3,926,638 + Pf1N1B4_3518 0.86 -1.6 3,926,638 + Pf1N1B4_3518 0.86 -3.1 3,926,639 - Pf1N1B4_3518 0.86 -2.0 3,926,639 - Pf1N1B4_3518 0.86 +0.6 3,926,639 - Pf1N1B4_3518 0.86 +0.1 3,926,639 - Pf1N1B4_3518 0.86 -2.8 3,926,639 - Pf1N1B4_3518 0.86 -2.0 3,926,708 + -1.9 3,926,745 + +1.3 3,926,767 + -1.0 3,926,767 + -0.6 3,926,767 + +0.4 3,926,767 + +0.3 3,926,767 + +0.2 3,926,767 + -2.6 3,926,767 + -0.8 3,926,767 + -1.7 3,926,767 + -0.6 3,926,768 - -2.7 3,926,768 - -0.3 3,926,771 + +1.0 3,926,781 + +2.1 3,926,781 + +0.4 3,926,781 + +1.3 3,926,781 + +1.1 3,926,781 + +1.2 3,926,782 - +1.3 3,926,782 - -2.0 3,926,782 - -2.9 3,926,782 - +0.7 3,926,828 + +0.8 3,926,828 + -3.4 3,926,828 + +0.1 3,926,828 + -1.3 3,926,867 + Pf1N1B4_3519 0.19 -2.3 3,926,867 + Pf1N1B4_3519 0.19 -0.3 3,926,867 + +0.8 3,926,919 + Pf1N1B4_3519 0.34 -2.1 3,926,919 + Pf1N1B4_3519 0.34 -2.8 3,926,919 + Pf1N1B4_3519 0.34 -0.1 3,926,919 + Pf1N1B4_3519 0.34 -3.0 3,926,920 - Pf1N1B4_3519 0.34 -1.7 3,926,931 - Pf1N1B4_3519 0.37 -3.6 3,926,942 + Pf1N1B4_3519 0.41 -2.0 3,927,144 + -0.3 3,927,159 - -0.6 3,927,286 + Pf1N1B4_3520 0.31 +0.3 3,927,286 + Pf1N1B4_3520 0.31 -1.7 3,927,286 + Pf1N1B4_3520 0.31 -0.0 3,927,286 + Pf1N1B4_3520 0.31 -0.9 3,927,286 + Pf1N1B4_3520 0.31 +0.1 3,927,286 + Pf1N1B4_3520 0.31 +0.7 3,927,287 - Pf1N1B4_3520 0.31 -1.7 3,927,419 + Pf1N1B4_3520 0.60 +0.5 3,927,478 + Pf1N1B4_3520 0.74 -0.9 3,927,479 - Pf1N1B4_3520 0.74 +0.9 3,927,479 - Pf1N1B4_3520 0.74 -1.1 3,927,479 - Pf1N1B4_3520 0.74 -1.0 3,927,639 + -0.6 3,927,640 - -0.6 3,927,727 - -1.8 3,927,828 + +0.3 3,927,828 + +1.5 3,927,828 + -2.0 3,927,828 + +0.3 3,927,829 - +0.7 3,927,829 - +0.4 3,927,829 - +0.6
Or see this region's nucleotide sequence