Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_341 and Pf1N1B4_342 overlap by 4 nucleotides Pf1N1B4_342 and Pf1N1B4_343 overlap by 4 nucleotides Pf1N1B4_343 and Pf1N1B4_344 are separated by 226 nucleotides Pf1N1B4_344 and Pf1N1B4_345 are separated by 27 nucleotides
Pf1N1B4_341 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 405,093 to 405,983
_341
Pf1N1B4_342 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 405,980 to 406,873
_342
Pf1N1B4_343 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 406,870 to 407,946
_343
Pf1N1B4_344 - hypothetical protein, at 408,173 to 408,412
_344
Pf1N1B4_345 - Integration host factor beta subunit, at 408,440 to 408,736
_345
Position (kb) 406
407
408 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 405.966 kb on - strand at 405.985 kb on + strand at 405.985 kb on + strand at 406.896 kb on - strand at 406.943 kb on - strand at 406.943 kb on - strand at 407.055 kb on - strand, within Pf1N1B4_343 at 407.088 kb on - strand at 407.088 kb on - strand, within Pf1N1B4_343 at 407.158 kb on - strand, within Pf1N1B4_343 at 407.205 kb on - strand, within Pf1N1B4_343 at 407.205 kb on - strand, within Pf1N1B4_343 at 407.205 kb on - strand, within Pf1N1B4_343 at 407.205 kb on - strand, within Pf1N1B4_343 at 407.241 kb on - strand, within Pf1N1B4_343 at 407.241 kb on - strand, within Pf1N1B4_343 at 407.241 kb on - strand, within Pf1N1B4_343 at 407.241 kb on - strand, within Pf1N1B4_343 at 407.268 kb on - strand, within Pf1N1B4_343 at 407.268 kb on - strand, within Pf1N1B4_343 at 407.418 kb on - strand, within Pf1N1B4_343 at 407.418 kb on - strand, within Pf1N1B4_343 at 407.422 kb on - strand, within Pf1N1B4_343 at 407.472 kb on - strand, within Pf1N1B4_343 at 407.557 kb on - strand, within Pf1N1B4_343 at 407.557 kb on - strand, within Pf1N1B4_343 at 407.841 kb on - strand at 407.963 kb on - strand at 408.095 kb on - strand at 408.101 kb on + strand at 408.102 kb on - strand at 408.113 kb on + strand at 408.113 kb on + strand at 408.114 kb on - strand at 408.341 kb on + strand, within Pf1N1B4_344 at 408.380 kb on + strand, within Pf1N1B4_344 at 408.380 kb on + strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.381 kb on - strand, within Pf1N1B4_344 at 408.392 kb on + strand at 408.392 kb on + strand at 408.393 kb on - strand at 408.393 kb on - strand at 408.393 kb on - strand at 408.574 kb on - strand, within Pf1N1B4_345 at 408.825 kb on + strand at 408.825 kb on + strand at 408.825 kb on + strand at 408.826 kb on - strand at 408.826 kb on - strand at 408.892 kb on + strand at 408.893 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 405,966 - -1.2 405,985 + -1.5 405,985 + -0.8 406,896 - -0.7 406,943 - -1.0 406,943 - -0.3 407,055 - Pf1N1B4_343 0.17 +0.2 407,088 - +0.0 407,088 - Pf1N1B4_343 0.20 -2.6 407,158 - Pf1N1B4_343 0.27 -1.6 407,205 - Pf1N1B4_343 0.31 +0.9 407,205 - Pf1N1B4_343 0.31 +0.4 407,205 - Pf1N1B4_343 0.31 -1.0 407,205 - Pf1N1B4_343 0.31 -0.8 407,241 - Pf1N1B4_343 0.34 +1.7 407,241 - Pf1N1B4_343 0.34 +0.7 407,241 - Pf1N1B4_343 0.34 -0.3 407,241 - Pf1N1B4_343 0.34 +0.7 407,268 - Pf1N1B4_343 0.37 +1.2 407,268 - Pf1N1B4_343 0.37 -1.3 407,418 - Pf1N1B4_343 0.51 -1.4 407,418 - Pf1N1B4_343 0.51 +0.2 407,422 - Pf1N1B4_343 0.51 -0.3 407,472 - Pf1N1B4_343 0.56 -1.4 407,557 - Pf1N1B4_343 0.64 -2.0 407,557 - Pf1N1B4_343 0.64 -1.4 407,841 - -0.8 407,963 - +2.7 408,095 - +0.3 408,101 + -0.0 408,102 - -0.5 408,113 + -2.0 408,113 + -3.0 408,114 - -0.3 408,341 + Pf1N1B4_344 0.70 +0.8 408,380 + Pf1N1B4_344 0.86 -2.2 408,380 + Pf1N1B4_344 0.86 -0.1 408,381 - Pf1N1B4_344 0.87 +1.3 408,381 - Pf1N1B4_344 0.87 +0.0 408,381 - Pf1N1B4_344 0.87 +0.5 408,381 - +0.7 408,381 - Pf1N1B4_344 0.87 -0.2 408,381 - Pf1N1B4_344 0.87 -1.3 408,381 - Pf1N1B4_344 0.87 -2.3 408,381 - Pf1N1B4_344 0.87 +0.3 408,392 + +0.6 408,392 + -0.4 408,393 - -1.0 408,393 - -0.5 408,393 - +0.9 408,574 - Pf1N1B4_345 0.45 -0.2 408,825 + -2.0 408,825 + -1.0 408,825 + -1.3 408,826 - -2.3 408,826 - -1.5 408,892 + -0.8 408,893 - -0.7
Or see this region's nucleotide sequence