Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_339 and Pf1N1B4_340 overlap by 4 nucleotides Pf1N1B4_340 and Pf1N1B4_341 are separated by 56 nucleotides Pf1N1B4_341 and Pf1N1B4_342 overlap by 4 nucleotides Pf1N1B4_342 and Pf1N1B4_343 overlap by 4 nucleotides
Pf1N1B4_339 - UDP-glucose 4-epimerase (EC 5.1.3.2), at 403,154 to 404,116
_339
Pf1N1B4_340 - Alpha-L-Rha alpha-1,3-L-rhamnosyltransferase (EC 2.4.1.-), at 404,113 to 405,036
_340
Pf1N1B4_341 - Glucose-1-phosphate thymidylyltransferase (EC 2.7.7.24), at 405,093 to 405,983
_341
Pf1N1B4_342 - dTDP-4-dehydrorhamnose reductase (EC 1.1.1.133), at 405,980 to 406,873
_342
Pf1N1B4_343 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 406,870 to 407,946
_343
Position (kb) 405
406 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 404.132 kb on - strand at 404.155 kb on - strand at 404.155 kb on - strand at 404.219 kb on - strand, within Pf1N1B4_340 at 404.383 kb on - strand, within Pf1N1B4_340 at 404.427 kb on - strand, within Pf1N1B4_340 at 404.427 kb on - strand, within Pf1N1B4_340 at 404.460 kb on - strand, within Pf1N1B4_340 at 404.466 kb on - strand, within Pf1N1B4_340 at 404.555 kb on + strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand, within Pf1N1B4_340 at 404.556 kb on - strand at 404.559 kb on + strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.560 kb on - strand, within Pf1N1B4_340 at 404.588 kb on + strand, within Pf1N1B4_340 at 404.589 kb on - strand, within Pf1N1B4_340 at 404.589 kb on - strand, within Pf1N1B4_340 at 404.589 kb on - strand, within Pf1N1B4_340 at 404.669 kb on + strand, within Pf1N1B4_340 at 404.669 kb on + strand, within Pf1N1B4_340 at 404.921 kb on + strand, within Pf1N1B4_340 at 404.921 kb on + strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.922 kb on - strand, within Pf1N1B4_340 at 404.993 kb on + strand at 404.993 kb on + strand at 404.993 kb on + strand at 404.994 kb on - strand at 404.994 kb on - strand at 404.994 kb on - strand at 404.994 kb on - strand at 405.029 kb on - strand at 405.029 kb on - strand at 405.128 kb on - strand at 405.128 kb on - strand at 405.128 kb on - strand at 405.359 kb on - strand at 405.443 kb on - strand, within Pf1N1B4_341 at 405.443 kb on - strand, within Pf1N1B4_341 at 405.443 kb on - strand, within Pf1N1B4_341 at 405.448 kb on + strand, within Pf1N1B4_341 at 405.448 kb on + strand, within Pf1N1B4_341 at 405.448 kb on + strand, within Pf1N1B4_341 at 405.449 kb on - strand, within Pf1N1B4_341 at 405.476 kb on + strand, within Pf1N1B4_341 at 405.477 kb on - strand, within Pf1N1B4_341 at 405.574 kb on - strand, within Pf1N1B4_341 at 405.635 kb on - strand, within Pf1N1B4_341 at 405.635 kb on - strand, within Pf1N1B4_341 at 405.726 kb on - strand, within Pf1N1B4_341 at 405.740 kb on - strand, within Pf1N1B4_341 at 405.815 kb on + strand at 405.815 kb on + strand, within Pf1N1B4_341 at 405.816 kb on - strand, within Pf1N1B4_341 at 405.966 kb on - strand at 405.985 kb on + strand at 405.985 kb on + strand at 406.896 kb on - strand at 406.943 kb on - strand at 406.943 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 404,132 - -1.4 404,155 - -2.0 404,155 - +1.0 404,219 - Pf1N1B4_340 0.11 -0.2 404,383 - Pf1N1B4_340 0.29 -1.6 404,427 - Pf1N1B4_340 0.34 -1.1 404,427 - Pf1N1B4_340 0.34 -1.4 404,460 - Pf1N1B4_340 0.38 -1.0 404,466 - Pf1N1B4_340 0.38 -1.1 404,555 + Pf1N1B4_340 0.48 -0.0 404,556 - Pf1N1B4_340 0.48 -2.3 404,556 - Pf1N1B4_340 0.48 -0.6 404,556 - Pf1N1B4_340 0.48 +0.5 404,556 - Pf1N1B4_340 0.48 -1.9 404,556 - Pf1N1B4_340 0.48 -0.4 404,556 - Pf1N1B4_340 0.48 -2.6 404,556 - Pf1N1B4_340 0.48 -0.3 404,556 - -1.5 404,559 + Pf1N1B4_340 0.48 -1.7 404,560 - Pf1N1B4_340 0.48 -3.1 404,560 - Pf1N1B4_340 0.48 -1.3 404,560 - Pf1N1B4_340 0.48 -1.9 404,560 - Pf1N1B4_340 0.48 +0.0 404,560 - Pf1N1B4_340 0.48 -1.2 404,588 + Pf1N1B4_340 0.51 -1.9 404,589 - Pf1N1B4_340 0.52 -0.0 404,589 - Pf1N1B4_340 0.52 -1.9 404,589 - Pf1N1B4_340 0.52 -0.6 404,669 + Pf1N1B4_340 0.60 -2.8 404,669 + Pf1N1B4_340 0.60 -2.9 404,921 + Pf1N1B4_340 0.87 -2.7 404,921 + Pf1N1B4_340 0.87 +0.7 404,922 - Pf1N1B4_340 0.88 +0.2 404,922 - Pf1N1B4_340 0.88 +1.8 404,922 - Pf1N1B4_340 0.88 -1.8 404,922 - Pf1N1B4_340 0.88 -2.2 404,922 - Pf1N1B4_340 0.88 -1.1 404,922 - Pf1N1B4_340 0.88 -0.2 404,993 + +0.0 404,993 + -2.7 404,993 + -2.2 404,994 - -0.5 404,994 - -2.5 404,994 - -2.3 404,994 - +0.6 405,029 - -1.5 405,029 - -1.4 405,128 - +0.9 405,128 - +1.9 405,128 - -0.5 405,359 - -1.4 405,443 - Pf1N1B4_341 0.39 -1.6 405,443 - Pf1N1B4_341 0.39 -0.7 405,443 - Pf1N1B4_341 0.39 -1.3 405,448 + Pf1N1B4_341 0.40 -1.6 405,448 + Pf1N1B4_341 0.40 -1.9 405,448 + Pf1N1B4_341 0.40 +0.6 405,449 - Pf1N1B4_341 0.40 -0.6 405,476 + Pf1N1B4_341 0.43 -0.9 405,477 - Pf1N1B4_341 0.43 -0.8 405,574 - Pf1N1B4_341 0.54 -1.1 405,635 - Pf1N1B4_341 0.61 -1.9 405,635 - Pf1N1B4_341 0.61 -0.6 405,726 - Pf1N1B4_341 0.71 -0.3 405,740 - Pf1N1B4_341 0.73 -0.0 405,815 + -1.4 405,815 + Pf1N1B4_341 0.81 -1.3 405,816 - Pf1N1B4_341 0.81 -1.5 405,966 - -1.2 405,985 + -1.5 405,985 + -0.8 406,896 - -0.7 406,943 - -1.0 406,943 - -0.3
Or see this region's nucleotide sequence