Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3380 and Pf1N1B4_3381 overlap by 8 nucleotides Pf1N1B4_3381 and Pf1N1B4_3382 overlap by 4 nucleotides
Pf1N1B4_3380 - D-glucarate permease, at 3,767,934 to 3,769,205
_3380
Pf1N1B4_3381 - Y4mH, at 3,769,198 to 3,770,154
_3381
Pf1N1B4_3382 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 3,770,151 to 3,771,155
_3382
Position (kb)
3769
3770
3771 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3768.211 kb on + strand, within Pf1N1B4_3380 at 3768.408 kb on + strand, within Pf1N1B4_3380 at 3768.409 kb on - strand, within Pf1N1B4_3380 at 3768.672 kb on + strand, within Pf1N1B4_3380 at 3768.672 kb on + strand, within Pf1N1B4_3380 at 3768.672 kb on + strand, within Pf1N1B4_3380 at 3768.672 kb on + strand, within Pf1N1B4_3380 at 3768.672 kb on + strand, within Pf1N1B4_3380 at 3768.673 kb on - strand, within Pf1N1B4_3380 at 3769.171 kb on + strand at 3769.171 kb on + strand at 3769.172 kb on - strand at 3769.337 kb on - strand, within Pf1N1B4_3381 at 3769.351 kb on + strand, within Pf1N1B4_3381 at 3769.351 kb on + strand, within Pf1N1B4_3381 at 3769.351 kb on + strand, within Pf1N1B4_3381 at 3769.352 kb on - strand, within Pf1N1B4_3381 at 3769.393 kb on + strand, within Pf1N1B4_3381 at 3769.393 kb on + strand, within Pf1N1B4_3381 at 3769.393 kb on + strand, within Pf1N1B4_3381 at 3769.393 kb on + strand at 3769.393 kb on + strand, within Pf1N1B4_3381 at 3769.393 kb on + strand, within Pf1N1B4_3381 at 3769.393 kb on + strand, within Pf1N1B4_3381 at 3769.394 kb on - strand, within Pf1N1B4_3381 at 3769.394 kb on - strand, within Pf1N1B4_3381 at 3769.394 kb on - strand, within Pf1N1B4_3381 at 3769.394 kb on - strand, within Pf1N1B4_3381 at 3769.394 kb on - strand, within Pf1N1B4_3381 at 3769.394 kb on - strand, within Pf1N1B4_3381 at 3770.012 kb on - strand, within Pf1N1B4_3381 at 3770.012 kb on - strand, within Pf1N1B4_3381 at 3770.092 kb on + strand at 3770.379 kb on + strand, within Pf1N1B4_3382 at 3770.380 kb on - strand, within Pf1N1B4_3382 at 3770.587 kb on + strand, within Pf1N1B4_3382 at 3770.588 kb on - strand at 3770.834 kb on + strand, within Pf1N1B4_3382 at 3770.958 kb on + strand, within Pf1N1B4_3382 at 3770.958 kb on + strand, within Pf1N1B4_3382 at 3770.958 kb on + strand, within Pf1N1B4_3382 at 3770.958 kb on + strand, within Pf1N1B4_3382 at 3770.958 kb on + strand, within Pf1N1B4_3382 at 3770.958 kb on + strand, within Pf1N1B4_3382 at 3770.959 kb on - strand, within Pf1N1B4_3382 at 3770.959 kb on - strand, within Pf1N1B4_3382 at 3770.959 kb on - strand, within Pf1N1B4_3382 at 3770.959 kb on - strand, within Pf1N1B4_3382 at 3770.959 kb on - strand at 3770.959 kb on - strand, within Pf1N1B4_3382 at 3771.150 kb on + strand at 3771.150 kb on + strand at 3771.150 kb on + strand at 3771.150 kb on + strand at 3771.151 kb on - strand at 3771.151 kb on - strand at 3771.151 kb on - strand at 3771.151 kb on - strand at 3771.151 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 3,768,211 + Pf1N1B4_3380 0.22 +1.4 3,768,408 + Pf1N1B4_3380 0.37 +0.7 3,768,409 - Pf1N1B4_3380 0.37 -0.1 3,768,672 + Pf1N1B4_3380 0.58 -0.1 3,768,672 + Pf1N1B4_3380 0.58 -1.0 3,768,672 + Pf1N1B4_3380 0.58 -2.2 3,768,672 + Pf1N1B4_3380 0.58 -0.2 3,768,672 + Pf1N1B4_3380 0.58 +0.9 3,768,673 - Pf1N1B4_3380 0.58 -0.6 3,769,171 + +1.4 3,769,171 + +1.8 3,769,172 - +2.3 3,769,337 - Pf1N1B4_3381 0.15 -0.4 3,769,351 + Pf1N1B4_3381 0.16 -1.0 3,769,351 + Pf1N1B4_3381 0.16 +0.5 3,769,351 + Pf1N1B4_3381 0.16 +1.1 3,769,352 - Pf1N1B4_3381 0.16 -1.8 3,769,393 + Pf1N1B4_3381 0.20 -2.6 3,769,393 + Pf1N1B4_3381 0.20 -0.6 3,769,393 + Pf1N1B4_3381 0.20 -1.2 3,769,393 + -0.5 3,769,393 + Pf1N1B4_3381 0.20 -0.5 3,769,393 + Pf1N1B4_3381 0.20 -0.2 3,769,393 + Pf1N1B4_3381 0.20 -2.3 3,769,394 - Pf1N1B4_3381 0.20 +0.1 3,769,394 - Pf1N1B4_3381 0.20 -0.6 3,769,394 - Pf1N1B4_3381 0.20 -0.9 3,769,394 - Pf1N1B4_3381 0.20 +0.1 3,769,394 - Pf1N1B4_3381 0.20 -0.0 3,769,394 - Pf1N1B4_3381 0.20 +2.3 3,770,012 - Pf1N1B4_3381 0.85 +0.4 3,770,012 - Pf1N1B4_3381 0.85 -2.1 3,770,092 + -0.2 3,770,379 + Pf1N1B4_3382 0.23 +0.9 3,770,380 - Pf1N1B4_3382 0.23 -0.4 3,770,587 + Pf1N1B4_3382 0.43 +0.3 3,770,588 - -0.7 3,770,834 + Pf1N1B4_3382 0.68 +2.0 3,770,958 + Pf1N1B4_3382 0.80 -0.8 3,770,958 + Pf1N1B4_3382 0.80 +0.1 3,770,958 + Pf1N1B4_3382 0.80 +0.7 3,770,958 + Pf1N1B4_3382 0.80 -2.0 3,770,958 + Pf1N1B4_3382 0.80 -0.1 3,770,958 + Pf1N1B4_3382 0.80 +1.1 3,770,959 - Pf1N1B4_3382 0.80 +0.8 3,770,959 - Pf1N1B4_3382 0.80 -0.1 3,770,959 - Pf1N1B4_3382 0.80 -0.0 3,770,959 - Pf1N1B4_3382 0.80 +0.1 3,770,959 - +0.2 3,770,959 - Pf1N1B4_3382 0.80 -0.3 3,771,150 + -1.2 3,771,150 + +0.5 3,771,150 + -1.2 3,771,150 + +1.6 3,771,151 - -2.3 3,771,151 - -1.2 3,771,151 - +0.4 3,771,151 - -3.3 3,771,151 - -0.2
Or see this region's nucleotide sequence