Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3339 and Pf1N1B4_3340 are separated by 147 nucleotides Pf1N1B4_3340 and Pf1N1B4_3341 are separated by 15 nucleotides
Pf1N1B4_3339 - Transcriptional regulator, LysR family, at 3,725,520 to 3,726,455
_3339
Pf1N1B4_3340 - putative membrane protein, at 3,726,603 to 3,727,550
_3340
Pf1N1B4_3341 - Cyanate transport protein CynX, at 3,727,566 to 3,728,750
_3341
Position (kb)
3726
3727
3728 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3725.747 kb on + strand, within Pf1N1B4_3339 at 3725.749 kb on + strand, within Pf1N1B4_3339 at 3725.750 kb on - strand, within Pf1N1B4_3339 at 3725.750 kb on - strand, within Pf1N1B4_3339 at 3725.769 kb on + strand, within Pf1N1B4_3339 at 3725.769 kb on + strand, within Pf1N1B4_3339 at 3725.769 kb on + strand, within Pf1N1B4_3339 at 3725.770 kb on - strand, within Pf1N1B4_3339 at 3725.813 kb on + strand, within Pf1N1B4_3339 at 3725.813 kb on + strand, within Pf1N1B4_3339 at 3725.813 kb on + strand, within Pf1N1B4_3339 at 3725.814 kb on - strand, within Pf1N1B4_3339 at 3726.127 kb on + strand, within Pf1N1B4_3339 at 3726.143 kb on + strand at 3726.234 kb on - strand, within Pf1N1B4_3339 at 3726.253 kb on + strand, within Pf1N1B4_3339 at 3726.253 kb on + strand, within Pf1N1B4_3339 at 3726.253 kb on + strand, within Pf1N1B4_3339 at 3726.254 kb on - strand, within Pf1N1B4_3339 at 3726.254 kb on - strand, within Pf1N1B4_3339 at 3726.391 kb on + strand at 3726.392 kb on - strand at 3726.422 kb on + strand at 3726.435 kb on - strand at 3726.435 kb on - strand at 3726.443 kb on + strand at 3726.443 kb on + strand at 3726.444 kb on - strand at 3726.444 kb on - strand at 3726.547 kb on - strand at 3726.547 kb on - strand at 3726.675 kb on + strand at 3726.676 kb on - strand at 3726.705 kb on + strand, within Pf1N1B4_3340 at 3726.787 kb on + strand, within Pf1N1B4_3340 at 3726.787 kb on + strand, within Pf1N1B4_3340 at 3726.787 kb on + strand, within Pf1N1B4_3340 at 3726.787 kb on + strand, within Pf1N1B4_3340 at 3726.788 kb on - strand, within Pf1N1B4_3340 at 3726.788 kb on - strand at 3726.788 kb on - strand, within Pf1N1B4_3340 at 3726.788 kb on - strand, within Pf1N1B4_3340 at 3726.788 kb on - strand, within Pf1N1B4_3340 at 3726.788 kb on - strand, within Pf1N1B4_3340 at 3726.793 kb on + strand, within Pf1N1B4_3340 at 3726.793 kb on + strand, within Pf1N1B4_3340 at 3726.794 kb on - strand, within Pf1N1B4_3340 at 3726.794 kb on - strand, within Pf1N1B4_3340 at 3726.794 kb on - strand, within Pf1N1B4_3340 at 3726.877 kb on + strand, within Pf1N1B4_3340 at 3726.877 kb on + strand, within Pf1N1B4_3340 at 3726.878 kb on - strand at 3726.878 kb on - strand, within Pf1N1B4_3340 at 3726.878 kb on - strand at 3726.954 kb on + strand, within Pf1N1B4_3340 at 3726.955 kb on - strand, within Pf1N1B4_3340 at 3726.977 kb on + strand, within Pf1N1B4_3340 at 3726.977 kb on + strand, within Pf1N1B4_3340 at 3726.977 kb on + strand, within Pf1N1B4_3340 at 3726.977 kb on + strand, within Pf1N1B4_3340 at 3727.089 kb on + strand, within Pf1N1B4_3340 at 3727.097 kb on - strand, within Pf1N1B4_3340 at 3727.176 kb on + strand, within Pf1N1B4_3340 at 3727.177 kb on - strand at 3727.279 kb on + strand, within Pf1N1B4_3340 at 3727.280 kb on - strand, within Pf1N1B4_3340 at 3727.309 kb on + strand, within Pf1N1B4_3340 at 3727.310 kb on - strand, within Pf1N1B4_3340 at 3727.325 kb on + strand, within Pf1N1B4_3340 at 3727.387 kb on - strand, within Pf1N1B4_3340 at 3727.401 kb on + strand, within Pf1N1B4_3340 at 3727.969 kb on - strand, within Pf1N1B4_3341 at 3728.047 kb on + strand, within Pf1N1B4_3341 at 3728.047 kb on + strand, within Pf1N1B4_3341 at 3728.047 kb on + strand, within Pf1N1B4_3341 at 3728.047 kb on + strand, within Pf1N1B4_3341 at 3728.047 kb on + strand, within Pf1N1B4_3341 at 3728.048 kb on - strand at 3728.048 kb on - strand, within Pf1N1B4_3341 at 3728.048 kb on - strand, within Pf1N1B4_3341 at 3728.050 kb on + strand, within Pf1N1B4_3341 at 3728.050 kb on + strand, within Pf1N1B4_3341 at 3728.050 kb on + strand, within Pf1N1B4_3341 at 3728.051 kb on - strand at 3728.080 kb on + strand, within Pf1N1B4_3341 at 3728.081 kb on - strand at 3728.081 kb on - strand, within Pf1N1B4_3341 at 3728.081 kb on - strand, within Pf1N1B4_3341 at 3728.329 kb on + strand, within Pf1N1B4_3341 at 3728.329 kb on + strand, within Pf1N1B4_3341 at 3728.330 kb on - strand at 3728.330 kb on - strand, within Pf1N1B4_3341
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 3,725,747 + Pf1N1B4_3339 0.24 -0.8 3,725,749 + Pf1N1B4_3339 0.24 +0.8 3,725,750 - Pf1N1B4_3339 0.25 -0.9 3,725,750 - Pf1N1B4_3339 0.25 +0.8 3,725,769 + Pf1N1B4_3339 0.27 -1.3 3,725,769 + Pf1N1B4_3339 0.27 +0.5 3,725,769 + Pf1N1B4_3339 0.27 -1.6 3,725,770 - Pf1N1B4_3339 0.27 +0.2 3,725,813 + Pf1N1B4_3339 0.31 +0.2 3,725,813 + Pf1N1B4_3339 0.31 +1.6 3,725,813 + Pf1N1B4_3339 0.31 +1.3 3,725,814 - Pf1N1B4_3339 0.31 -0.2 3,726,127 + Pf1N1B4_3339 0.65 +0.9 3,726,143 + -0.5 3,726,234 - Pf1N1B4_3339 0.76 -1.3 3,726,253 + Pf1N1B4_3339 0.78 -1.6 3,726,253 + Pf1N1B4_3339 0.78 -1.6 3,726,253 + Pf1N1B4_3339 0.78 -0.5 3,726,254 - Pf1N1B4_3339 0.78 -0.5 3,726,254 - Pf1N1B4_3339 0.78 -1.0 3,726,391 + -0.8 3,726,392 - -0.7 3,726,422 + -0.2 3,726,435 - +1.6 3,726,435 - -3.0 3,726,443 + -1.5 3,726,443 + -1.5 3,726,444 - +1.2 3,726,444 - -1.7 3,726,547 - +0.1 3,726,547 - +0.4 3,726,675 + -0.0 3,726,676 - +1.0 3,726,705 + Pf1N1B4_3340 0.11 -1.9 3,726,787 + Pf1N1B4_3340 0.19 +0.0 3,726,787 + Pf1N1B4_3340 0.19 +1.5 3,726,787 + Pf1N1B4_3340 0.19 -1.0 3,726,787 + Pf1N1B4_3340 0.19 -1.3 3,726,788 - Pf1N1B4_3340 0.20 -2.0 3,726,788 - -0.2 3,726,788 - Pf1N1B4_3340 0.20 -1.8 3,726,788 - Pf1N1B4_3340 0.20 -1.2 3,726,788 - Pf1N1B4_3340 0.20 -0.8 3,726,788 - Pf1N1B4_3340 0.20 -2.5 3,726,793 + Pf1N1B4_3340 0.20 +0.4 3,726,793 + Pf1N1B4_3340 0.20 -0.5 3,726,794 - Pf1N1B4_3340 0.20 -1.2 3,726,794 - Pf1N1B4_3340 0.20 -1.9 3,726,794 - Pf1N1B4_3340 0.20 -2.7 3,726,877 + Pf1N1B4_3340 0.29 +0.6 3,726,877 + Pf1N1B4_3340 0.29 -2.4 3,726,878 - -1.5 3,726,878 - Pf1N1B4_3340 0.29 -1.0 3,726,878 - -1.0 3,726,954 + Pf1N1B4_3340 0.37 -2.8 3,726,955 - Pf1N1B4_3340 0.37 -1.3 3,726,977 + Pf1N1B4_3340 0.39 +1.7 3,726,977 + Pf1N1B4_3340 0.39 +0.7 3,726,977 + Pf1N1B4_3340 0.39 +0.3 3,726,977 + Pf1N1B4_3340 0.39 -1.6 3,727,089 + Pf1N1B4_3340 0.51 -1.0 3,727,097 - Pf1N1B4_3340 0.52 +0.7 3,727,176 + Pf1N1B4_3340 0.60 +1.0 3,727,177 - -0.9 3,727,279 + Pf1N1B4_3340 0.71 -1.3 3,727,280 - Pf1N1B4_3340 0.71 -0.7 3,727,309 + Pf1N1B4_3340 0.74 +0.9 3,727,310 - Pf1N1B4_3340 0.75 +0.1 3,727,325 + Pf1N1B4_3340 0.76 -1.6 3,727,387 - Pf1N1B4_3340 0.83 +0.8 3,727,401 + Pf1N1B4_3340 0.84 -2.7 3,727,969 - Pf1N1B4_3341 0.34 +0.5 3,728,047 + Pf1N1B4_3341 0.41 -0.8 3,728,047 + Pf1N1B4_3341 0.41 +1.0 3,728,047 + Pf1N1B4_3341 0.41 -1.3 3,728,047 + Pf1N1B4_3341 0.41 -0.5 3,728,047 + Pf1N1B4_3341 0.41 -1.2 3,728,048 - +0.8 3,728,048 - Pf1N1B4_3341 0.41 -1.5 3,728,048 - Pf1N1B4_3341 0.41 +0.5 3,728,050 + Pf1N1B4_3341 0.41 +0.6 3,728,050 + Pf1N1B4_3341 0.41 -1.2 3,728,050 + Pf1N1B4_3341 0.41 +1.1 3,728,051 - +0.7 3,728,080 + Pf1N1B4_3341 0.43 -0.1 3,728,081 - +1.3 3,728,081 - Pf1N1B4_3341 0.43 +1.3 3,728,081 - Pf1N1B4_3341 0.43 +0.9 3,728,329 + Pf1N1B4_3341 0.64 +0.3 3,728,329 + Pf1N1B4_3341 0.64 -2.4 3,728,330 - -1.2 3,728,330 - Pf1N1B4_3341 0.64 -1.5
Or see this region's nucleotide sequence