Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3340

Experiment: Minimal media pH9

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3339 and Pf1N1B4_3340 are separated by 147 nucleotidesPf1N1B4_3340 and Pf1N1B4_3341 are separated by 15 nucleotides Pf1N1B4_3339 - Transcriptional regulator, LysR family, at 3,725,520 to 3,726,455 _3339 Pf1N1B4_3340 - putative membrane protein, at 3,726,603 to 3,727,550 _3340 Pf1N1B4_3341 - Cyanate transport protein CynX, at 3,727,566 to 3,728,750 _3341 Position (kb) 3726 3727 3728Strain fitness (log2 ratio) -3 -2 -1 0 1at 3725.747 kb on + strand, within Pf1N1B4_3339at 3725.749 kb on + strand, within Pf1N1B4_3339at 3725.750 kb on - strand, within Pf1N1B4_3339at 3725.750 kb on - strand, within Pf1N1B4_3339at 3725.769 kb on + strand, within Pf1N1B4_3339at 3725.769 kb on + strand, within Pf1N1B4_3339at 3725.769 kb on + strand, within Pf1N1B4_3339at 3725.770 kb on - strand, within Pf1N1B4_3339at 3725.813 kb on + strand, within Pf1N1B4_3339at 3725.813 kb on + strand, within Pf1N1B4_3339at 3725.813 kb on + strand, within Pf1N1B4_3339at 3725.814 kb on - strand, within Pf1N1B4_3339at 3726.127 kb on + strand, within Pf1N1B4_3339at 3726.143 kb on + strandat 3726.234 kb on - strand, within Pf1N1B4_3339at 3726.253 kb on + strand, within Pf1N1B4_3339at 3726.253 kb on + strand, within Pf1N1B4_3339at 3726.253 kb on + strand, within Pf1N1B4_3339at 3726.254 kb on - strand, within Pf1N1B4_3339at 3726.254 kb on - strand, within Pf1N1B4_3339at 3726.391 kb on + strandat 3726.392 kb on - strandat 3726.422 kb on + strandat 3726.435 kb on - strandat 3726.435 kb on - strandat 3726.443 kb on + strandat 3726.443 kb on + strandat 3726.444 kb on - strandat 3726.444 kb on - strandat 3726.547 kb on - strandat 3726.547 kb on - strandat 3726.675 kb on + strandat 3726.676 kb on - strandat 3726.705 kb on + strand, within Pf1N1B4_3340at 3726.787 kb on + strand, within Pf1N1B4_3340at 3726.787 kb on + strand, within Pf1N1B4_3340at 3726.787 kb on + strand, within Pf1N1B4_3340at 3726.787 kb on + strand, within Pf1N1B4_3340at 3726.788 kb on - strand, within Pf1N1B4_3340at 3726.788 kb on - strandat 3726.788 kb on - strand, within Pf1N1B4_3340at 3726.788 kb on - strand, within Pf1N1B4_3340at 3726.788 kb on - strand, within Pf1N1B4_3340at 3726.788 kb on - strand, within Pf1N1B4_3340at 3726.793 kb on + strand, within Pf1N1B4_3340at 3726.793 kb on + strand, within Pf1N1B4_3340at 3726.794 kb on - strand, within Pf1N1B4_3340at 3726.794 kb on - strand, within Pf1N1B4_3340at 3726.794 kb on - strand, within Pf1N1B4_3340at 3726.877 kb on + strand, within Pf1N1B4_3340at 3726.877 kb on + strand, within Pf1N1B4_3340at 3726.878 kb on - strandat 3726.878 kb on - strand, within Pf1N1B4_3340at 3726.878 kb on - strandat 3726.954 kb on + strand, within Pf1N1B4_3340at 3726.955 kb on - strand, within Pf1N1B4_3340at 3726.977 kb on + strand, within Pf1N1B4_3340at 3726.977 kb on + strand, within Pf1N1B4_3340at 3726.977 kb on + strand, within Pf1N1B4_3340at 3726.977 kb on + strand, within Pf1N1B4_3340at 3727.089 kb on + strand, within Pf1N1B4_3340at 3727.097 kb on - strand, within Pf1N1B4_3340at 3727.176 kb on + strand, within Pf1N1B4_3340at 3727.177 kb on - strandat 3727.279 kb on + strand, within Pf1N1B4_3340at 3727.280 kb on - strand, within Pf1N1B4_3340at 3727.309 kb on + strand, within Pf1N1B4_3340at 3727.310 kb on - strand, within Pf1N1B4_3340at 3727.325 kb on + strand, within Pf1N1B4_3340at 3727.387 kb on - strand, within Pf1N1B4_3340at 3727.401 kb on + strand, within Pf1N1B4_3340at 3727.969 kb on - strand, within Pf1N1B4_3341at 3728.047 kb on + strand, within Pf1N1B4_3341at 3728.047 kb on + strand, within Pf1N1B4_3341at 3728.047 kb on + strand, within Pf1N1B4_3341at 3728.047 kb on + strand, within Pf1N1B4_3341at 3728.047 kb on + strand, within Pf1N1B4_3341at 3728.048 kb on - strandat 3728.048 kb on - strand, within Pf1N1B4_3341at 3728.048 kb on - strand, within Pf1N1B4_3341at 3728.050 kb on + strand, within Pf1N1B4_3341at 3728.050 kb on + strand, within Pf1N1B4_3341at 3728.050 kb on + strand, within Pf1N1B4_3341at 3728.051 kb on - strandat 3728.080 kb on + strand, within Pf1N1B4_3341at 3728.081 kb on - strandat 3728.081 kb on - strand, within Pf1N1B4_3341at 3728.081 kb on - strand, within Pf1N1B4_3341at 3728.329 kb on + strand, within Pf1N1B4_3341at 3728.329 kb on + strand, within Pf1N1B4_3341at 3728.330 kb on - strandat 3728.330 kb on - strand, within Pf1N1B4_3341

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Per-strain Table

Position Strand Gene LocusTag Fraction Minimal media pH9
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3,725,747 + Pf1N1B4_3339 0.24 -0.8
3,725,749 + Pf1N1B4_3339 0.24 +0.8
3,725,750 - Pf1N1B4_3339 0.25 -0.9
3,725,750 - Pf1N1B4_3339 0.25 +0.8
3,725,769 + Pf1N1B4_3339 0.27 -1.3
3,725,769 + Pf1N1B4_3339 0.27 +0.5
3,725,769 + Pf1N1B4_3339 0.27 -1.6
3,725,770 - Pf1N1B4_3339 0.27 +0.2
3,725,813 + Pf1N1B4_3339 0.31 +0.2
3,725,813 + Pf1N1B4_3339 0.31 +1.6
3,725,813 + Pf1N1B4_3339 0.31 +1.3
3,725,814 - Pf1N1B4_3339 0.31 -0.2
3,726,127 + Pf1N1B4_3339 0.65 +0.9
3,726,143 + -0.5
3,726,234 - Pf1N1B4_3339 0.76 -1.3
3,726,253 + Pf1N1B4_3339 0.78 -1.6
3,726,253 + Pf1N1B4_3339 0.78 -1.6
3,726,253 + Pf1N1B4_3339 0.78 -0.5
3,726,254 - Pf1N1B4_3339 0.78 -0.5
3,726,254 - Pf1N1B4_3339 0.78 -1.0
3,726,391 + -0.8
3,726,392 - -0.7
3,726,422 + -0.2
3,726,435 - +1.6
3,726,435 - -3.0
3,726,443 + -1.5
3,726,443 + -1.5
3,726,444 - +1.2
3,726,444 - -1.7
3,726,547 - +0.1
3,726,547 - +0.4
3,726,675 + -0.0
3,726,676 - +1.0
3,726,705 + Pf1N1B4_3340 0.11 -1.9
3,726,787 + Pf1N1B4_3340 0.19 +0.0
3,726,787 + Pf1N1B4_3340 0.19 +1.5
3,726,787 + Pf1N1B4_3340 0.19 -1.0
3,726,787 + Pf1N1B4_3340 0.19 -1.3
3,726,788 - Pf1N1B4_3340 0.20 -2.0
3,726,788 - -0.2
3,726,788 - Pf1N1B4_3340 0.20 -1.8
3,726,788 - Pf1N1B4_3340 0.20 -1.2
3,726,788 - Pf1N1B4_3340 0.20 -0.8
3,726,788 - Pf1N1B4_3340 0.20 -2.5
3,726,793 + Pf1N1B4_3340 0.20 +0.4
3,726,793 + Pf1N1B4_3340 0.20 -0.5
3,726,794 - Pf1N1B4_3340 0.20 -1.2
3,726,794 - Pf1N1B4_3340 0.20 -1.9
3,726,794 - Pf1N1B4_3340 0.20 -2.7
3,726,877 + Pf1N1B4_3340 0.29 +0.6
3,726,877 + Pf1N1B4_3340 0.29 -2.4
3,726,878 - -1.5
3,726,878 - Pf1N1B4_3340 0.29 -1.0
3,726,878 - -1.0
3,726,954 + Pf1N1B4_3340 0.37 -2.8
3,726,955 - Pf1N1B4_3340 0.37 -1.3
3,726,977 + Pf1N1B4_3340 0.39 +1.7
3,726,977 + Pf1N1B4_3340 0.39 +0.7
3,726,977 + Pf1N1B4_3340 0.39 +0.3
3,726,977 + Pf1N1B4_3340 0.39 -1.6
3,727,089 + Pf1N1B4_3340 0.51 -1.0
3,727,097 - Pf1N1B4_3340 0.52 +0.7
3,727,176 + Pf1N1B4_3340 0.60 +1.0
3,727,177 - -0.9
3,727,279 + Pf1N1B4_3340 0.71 -1.3
3,727,280 - Pf1N1B4_3340 0.71 -0.7
3,727,309 + Pf1N1B4_3340 0.74 +0.9
3,727,310 - Pf1N1B4_3340 0.75 +0.1
3,727,325 + Pf1N1B4_3340 0.76 -1.6
3,727,387 - Pf1N1B4_3340 0.83 +0.8
3,727,401 + Pf1N1B4_3340 0.84 -2.7
3,727,969 - Pf1N1B4_3341 0.34 +0.5
3,728,047 + Pf1N1B4_3341 0.41 -0.8
3,728,047 + Pf1N1B4_3341 0.41 +1.0
3,728,047 + Pf1N1B4_3341 0.41 -1.3
3,728,047 + Pf1N1B4_3341 0.41 -0.5
3,728,047 + Pf1N1B4_3341 0.41 -1.2
3,728,048 - +0.8
3,728,048 - Pf1N1B4_3341 0.41 -1.5
3,728,048 - Pf1N1B4_3341 0.41 +0.5
3,728,050 + Pf1N1B4_3341 0.41 +0.6
3,728,050 + Pf1N1B4_3341 0.41 -1.2
3,728,050 + Pf1N1B4_3341 0.41 +1.1
3,728,051 - +0.7
3,728,080 + Pf1N1B4_3341 0.43 -0.1
3,728,081 - +1.3
3,728,081 - Pf1N1B4_3341 0.43 +1.3
3,728,081 - Pf1N1B4_3341 0.43 +0.9
3,728,329 + Pf1N1B4_3341 0.64 +0.3
3,728,329 + Pf1N1B4_3341 0.64 -2.4
3,728,330 - -1.2
3,728,330 - Pf1N1B4_3341 0.64 -1.5

Or see this region's nucleotide sequence