Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3148 and Pf1N1B4_3149 are separated by 87 nucleotides Pf1N1B4_3149 and Pf1N1B4_3150 are separated by 23 nucleotides Pf1N1B4_3150 and Pf1N1B4_3151 are separated by 75 nucleotides
Pf1N1B4_3148 - FIG074102: hypothetical protein, at 3,529,739 to 3,530,482
_3148
Pf1N1B4_3149 - hypothetical protein, at 3,530,570 to 3,530,698
_3149
Pf1N1B4_3150 - Transcriptional regulator, LuxR family, at 3,530,722 to 3,531,372
_3150
Pf1N1B4_3151 - Diacylglycerol kinase (EC 2.7.1.107), at 3,531,448 to 3,531,810
_3151
Position (kb)
3530
3531 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.619 kb on - strand at 3529.620 kb on + strand at 3529.621 kb on - strand at 3529.621 kb on - strand at 3529.621 kb on - strand at 3529.621 kb on - strand at 3529.681 kb on + strand at 3529.681 kb on + strand at 3529.869 kb on + strand, within Pf1N1B4_3148 at 3529.870 kb on - strand, within Pf1N1B4_3148 at 3529.927 kb on + strand, within Pf1N1B4_3148 at 3530.063 kb on + strand, within Pf1N1B4_3148 at 3530.064 kb on - strand, within Pf1N1B4_3148 at 3530.064 kb on - strand, within Pf1N1B4_3148 at 3530.084 kb on + strand at 3530.105 kb on - strand, within Pf1N1B4_3148 at 3530.360 kb on + strand, within Pf1N1B4_3148 at 3530.360 kb on + strand, within Pf1N1B4_3148 at 3530.360 kb on + strand, within Pf1N1B4_3148 at 3530.361 kb on - strand, within Pf1N1B4_3148 at 3530.384 kb on + strand, within Pf1N1B4_3148 at 3530.385 kb on - strand, within Pf1N1B4_3148 at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.493 kb on + strand at 3530.610 kb on + strand, within Pf1N1B4_3149 at 3530.611 kb on - strand, within Pf1N1B4_3149 at 3530.611 kb on - strand, within Pf1N1B4_3149 at 3530.731 kb on + strand at 3530.731 kb on + strand at 3530.731 kb on + strand at 3530.732 kb on - strand at 3530.732 kb on - strand at 3530.732 kb on - strand at 3530.732 kb on - strand at 3530.885 kb on + strand, within Pf1N1B4_3150 at 3530.886 kb on - strand, within Pf1N1B4_3150 at 3531.244 kb on + strand, within Pf1N1B4_3150 at 3531.370 kb on + strand at 3531.370 kb on + strand at 3531.370 kb on + strand at 3531.370 kb on + strand at 3531.370 kb on + strand at 3531.370 kb on + strand at 3531.379 kb on - strand at 3531.411 kb on + strand at 3531.411 kb on + strand at 3531.411 kb on + strand at 3531.443 kb on - strand at 3531.447 kb on + strand at 3531.447 kb on + strand at 3531.447 kb on + strand at 3531.447 kb on + strand at 3531.448 kb on - strand at 3531.448 kb on - strand at 3531.448 kb on - strand at 3531.448 kb on - strand at 3531.448 kb on - strand at 3531.448 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 3,529,618 + -0.5 3,529,618 + +0.4 3,529,618 + -2.2 3,529,618 + -0.1 3,529,618 + +1.5 3,529,618 + +1.2 3,529,619 - -0.8 3,529,620 + -0.9 3,529,621 - -1.9 3,529,621 - +1.4 3,529,621 - -0.3 3,529,621 - -0.0 3,529,681 + +0.3 3,529,681 + -0.1 3,529,869 + Pf1N1B4_3148 0.17 -1.4 3,529,870 - Pf1N1B4_3148 0.18 -1.6 3,529,927 + Pf1N1B4_3148 0.25 -1.7 3,530,063 + Pf1N1B4_3148 0.44 +0.4 3,530,064 - Pf1N1B4_3148 0.44 -1.8 3,530,064 - Pf1N1B4_3148 0.44 -2.6 3,530,084 + +2.1 3,530,105 - Pf1N1B4_3148 0.49 -1.7 3,530,360 + Pf1N1B4_3148 0.83 +1.1 3,530,360 + Pf1N1B4_3148 0.83 +1.9 3,530,360 + Pf1N1B4_3148 0.83 -0.4 3,530,361 - Pf1N1B4_3148 0.84 +0.6 3,530,384 + Pf1N1B4_3148 0.87 +1.8 3,530,385 - Pf1N1B4_3148 0.87 +0.6 3,530,454 + -0.2 3,530,454 + +0.1 3,530,454 + -1.4 3,530,454 + -1.5 3,530,454 + +1.1 3,530,493 + +0.8 3,530,610 + Pf1N1B4_3149 0.31 -0.4 3,530,611 - Pf1N1B4_3149 0.32 -0.0 3,530,611 - Pf1N1B4_3149 0.32 -2.1 3,530,731 + +1.4 3,530,731 + +0.9 3,530,731 + +0.3 3,530,732 - +1.4 3,530,732 - +0.9 3,530,732 - +0.3 3,530,732 - +1.6 3,530,885 + Pf1N1B4_3150 0.25 -1.0 3,530,886 - Pf1N1B4_3150 0.25 +1.8 3,531,244 + Pf1N1B4_3150 0.80 -0.1 3,531,370 + -0.8 3,531,370 + -1.8 3,531,370 + -1.9 3,531,370 + -1.4 3,531,370 + +1.3 3,531,370 + -0.3 3,531,379 - -0.8 3,531,411 + +2.0 3,531,411 + -0.1 3,531,411 + -1.7 3,531,443 - -1.1 3,531,447 + +0.4 3,531,447 + +0.1 3,531,447 + -0.4 3,531,447 + +1.9 3,531,448 - -2.6 3,531,448 - +0.3 3,531,448 - -0.5 3,531,448 - -1.3 3,531,448 - -0.7 3,531,448 - -1.4
Or see this region's nucleotide sequence