Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_307 and Pf1N1B4_308 are separated by 247 nucleotides Pf1N1B4_308 and Pf1N1B4_309 are separated by 44 nucleotides Pf1N1B4_309 and Pf1N1B4_310 are separated by 245 nucleotides
Pf1N1B4_307 - TesB-like acyl-CoA thioesterase 1, at 360,835 to 361,632
_307
Pf1N1B4_308 - FIG00956090: hypothetical protein, at 361,880 to 362,647
_308
Pf1N1B4_309 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 362,692 to 362,970
_309
Pf1N1B4_310 - UPF0028 protein YchK, at 363,216 to 364,256
_310
Position (kb) 361
362
363 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 360.950 kb on + strand, within Pf1N1B4_307 at 360.950 kb on + strand, within Pf1N1B4_307 at 360.950 kb on + strand, within Pf1N1B4_307 at 360.950 kb on + strand, within Pf1N1B4_307 at 360.951 kb on - strand, within Pf1N1B4_307 at 360.951 kb on - strand, within Pf1N1B4_307 at 360.951 kb on - strand, within Pf1N1B4_307 at 360.951 kb on - strand, within Pf1N1B4_307 at 361.043 kb on + strand, within Pf1N1B4_307 at 361.044 kb on - strand, within Pf1N1B4_307 at 361.086 kb on + strand, within Pf1N1B4_307 at 361.087 kb on - strand, within Pf1N1B4_307 at 361.237 kb on - strand, within Pf1N1B4_307 at 361.238 kb on + strand, within Pf1N1B4_307 at 361.238 kb on + strand, within Pf1N1B4_307 at 361.238 kb on + strand, within Pf1N1B4_307 at 361.238 kb on + strand, within Pf1N1B4_307 at 361.239 kb on - strand, within Pf1N1B4_307 at 361.239 kb on - strand, within Pf1N1B4_307 at 361.239 kb on - strand, within Pf1N1B4_307 at 361.239 kb on - strand, within Pf1N1B4_307 at 361.239 kb on - strand, within Pf1N1B4_307 at 361.518 kb on - strand, within Pf1N1B4_307 at 361.578 kb on + strand at 361.578 kb on + strand at 361.579 kb on - strand at 361.579 kb on - strand at 361.699 kb on + strand at 361.700 kb on - strand at 362.115 kb on + strand, within Pf1N1B4_308 at 362.398 kb on - strand, within Pf1N1B4_308 at 362.398 kb on - strand, within Pf1N1B4_308 at 362.398 kb on - strand, within Pf1N1B4_308 at 362.534 kb on + strand, within Pf1N1B4_308 at 362.534 kb on + strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.535 kb on - strand, within Pf1N1B4_308 at 362.659 kb on + strand at 362.659 kb on + strand at 362.684 kb on - strand at 362.684 kb on - strand at 362.684 kb on - strand at 362.684 kb on - strand at 362.684 kb on - strand at 362.720 kb on + strand, within Pf1N1B4_309 at 362.721 kb on - strand, within Pf1N1B4_309 at 362.721 kb on - strand, within Pf1N1B4_309 at 362.721 kb on - strand, within Pf1N1B4_309 at 362.759 kb on + strand, within Pf1N1B4_309 at 362.760 kb on - strand, within Pf1N1B4_309 at 362.760 kb on - strand, within Pf1N1B4_309 at 362.760 kb on - strand, within Pf1N1B4_309 at 362.760 kb on - strand, within Pf1N1B4_309 at 362.817 kb on - strand, within Pf1N1B4_309 at 362.988 kb on + strand at 362.988 kb on + strand at 362.988 kb on + strand at 362.988 kb on + strand at 362.992 kb on + strand at 362.992 kb on + strand at 362.992 kb on + strand at 362.992 kb on + strand at 362.993 kb on - strand at 362.993 kb on - strand at 362.993 kb on - strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.996 kb on + strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 362.997 kb on - strand at 363.008 kb on + strand at 363.008 kb on + strand at 363.008 kb on + strand at 363.008 kb on + strand at 363.008 kb on + strand at 363.009 kb on - strand at 363.009 kb on - strand at 363.009 kb on - strand at 363.009 kb on - strand at 363.009 kb on - strand at 363.082 kb on + strand at 363.082 kb on + strand at 363.082 kb on + strand at 363.082 kb on + strand at 363.083 kb on - strand at 363.083 kb on - strand at 363.083 kb on - strand at 363.083 kb on - strand at 363.360 kb on + strand, within Pf1N1B4_310 at 363.360 kb on + strand, within Pf1N1B4_310 at 363.360 kb on + strand, within Pf1N1B4_310 at 363.360 kb on + strand, within Pf1N1B4_310 at 363.361 kb on - strand, within Pf1N1B4_310 at 363.361 kb on - strand, within Pf1N1B4_310 at 363.361 kb on - strand at 363.411 kb on + strand, within Pf1N1B4_310 at 363.509 kb on + strand, within Pf1N1B4_310 at 363.509 kb on + strand, within Pf1N1B4_310 at 363.510 kb on - strand, within Pf1N1B4_310 at 363.510 kb on - strand, within Pf1N1B4_310 at 363.512 kb on + strand, within Pf1N1B4_310 at 363.512 kb on + strand, within Pf1N1B4_310 at 363.512 kb on + strand, within Pf1N1B4_310
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 360,950 + Pf1N1B4_307 0.14 +0.9 360,950 + Pf1N1B4_307 0.14 +0.5 360,950 + Pf1N1B4_307 0.14 +0.3 360,950 + Pf1N1B4_307 0.14 -1.5 360,951 - Pf1N1B4_307 0.15 -0.9 360,951 - Pf1N1B4_307 0.15 -1.1 360,951 - Pf1N1B4_307 0.15 +0.2 360,951 - Pf1N1B4_307 0.15 +0.9 361,043 + Pf1N1B4_307 0.26 -1.5 361,044 - Pf1N1B4_307 0.26 +0.5 361,086 + Pf1N1B4_307 0.31 +1.3 361,087 - Pf1N1B4_307 0.32 +0.7 361,237 - Pf1N1B4_307 0.50 +1.8 361,238 + Pf1N1B4_307 0.51 +1.9 361,238 + Pf1N1B4_307 0.51 +0.1 361,238 + Pf1N1B4_307 0.51 +0.9 361,238 + Pf1N1B4_307 0.51 +1.5 361,239 - Pf1N1B4_307 0.51 -0.3 361,239 - Pf1N1B4_307 0.51 +0.1 361,239 - Pf1N1B4_307 0.51 +1.0 361,239 - Pf1N1B4_307 0.51 -0.6 361,239 - Pf1N1B4_307 0.51 +1.2 361,518 - Pf1N1B4_307 0.86 +1.3 361,578 + -2.2 361,578 + +0.7 361,579 - +0.4 361,579 - -1.5 361,699 + -1.3 361,700 - +2.3 362,115 + Pf1N1B4_308 0.31 +0.1 362,398 - Pf1N1B4_308 0.67 -1.7 362,398 - Pf1N1B4_308 0.67 +0.0 362,398 - Pf1N1B4_308 0.67 +1.5 362,534 + Pf1N1B4_308 0.85 -0.9 362,534 + Pf1N1B4_308 0.85 +1.9 362,535 - Pf1N1B4_308 0.85 +0.7 362,535 - Pf1N1B4_308 0.85 -0.6 362,535 - Pf1N1B4_308 0.85 -2.1 362,535 - Pf1N1B4_308 0.85 -0.1 362,535 - Pf1N1B4_308 0.85 -2.9 362,535 - Pf1N1B4_308 0.85 +0.9 362,659 + -1.2 362,659 + +0.8 362,684 - +0.6 362,684 - -0.3 362,684 - +1.5 362,684 - +0.6 362,684 - -0.5 362,720 + Pf1N1B4_309 0.10 +4.0 362,721 - Pf1N1B4_309 0.10 +1.8 362,721 - Pf1N1B4_309 0.10 -2.3 362,721 - Pf1N1B4_309 0.10 -0.2 362,759 + Pf1N1B4_309 0.24 -2.2 362,760 - Pf1N1B4_309 0.24 -2.4 362,760 - Pf1N1B4_309 0.24 +0.3 362,760 - Pf1N1B4_309 0.24 -1.1 362,760 - Pf1N1B4_309 0.24 +1.3 362,817 - Pf1N1B4_309 0.45 +0.4 362,988 + -1.8 362,988 + -0.5 362,988 + +0.0 362,988 + -1.4 362,992 + +0.5 362,992 + +0.9 362,992 + -0.4 362,992 + +1.2 362,993 - -2.4 362,993 - +0.9 362,993 - -1.8 362,996 + +1.3 362,996 + +0.1 362,996 + +1.4 362,996 + -1.9 362,996 + +1.6 362,996 + -0.6 362,996 + -0.0 362,996 + +0.7 362,996 + -1.5 362,996 + -1.7 362,996 + -0.1 362,996 + +0.3 362,997 - -1.2 362,997 - -0.6 362,997 - +0.2 362,997 - -0.6 362,997 - -2.2 362,997 - +1.0 362,997 - -2.2 362,997 - +1.0 362,997 - -0.8 363,008 + +1.2 363,008 + -0.4 363,008 + -1.0 363,008 + -0.9 363,008 + +0.0 363,009 - +0.2 363,009 - -0.1 363,009 - +2.5 363,009 - -2.5 363,009 - -0.4 363,082 + -1.0 363,082 + +0.2 363,082 + +2.6 363,082 + +0.6 363,083 - -0.1 363,083 - -1.4 363,083 - -0.9 363,083 - +1.4 363,360 + Pf1N1B4_310 0.14 +0.2 363,360 + Pf1N1B4_310 0.14 +0.7 363,360 + Pf1N1B4_310 0.14 -1.0 363,360 + Pf1N1B4_310 0.14 +1.4 363,361 - Pf1N1B4_310 0.14 +1.3 363,361 - Pf1N1B4_310 0.14 -1.6 363,361 - -1.6 363,411 + Pf1N1B4_310 0.19 -1.1 363,509 + Pf1N1B4_310 0.28 +0.5 363,509 + Pf1N1B4_310 0.28 +0.1 363,510 - Pf1N1B4_310 0.28 -0.2 363,510 - Pf1N1B4_310 0.28 +0.9 363,512 + Pf1N1B4_310 0.28 +0.9 363,512 + Pf1N1B4_310 0.28 -0.4 363,512 + Pf1N1B4_310 0.28 +0.3
Or see this region's nucleotide sequence