Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3018 and Pf1N1B4_3019 are separated by 146 nucleotides Pf1N1B4_3019 and Pf1N1B4_3020 are separated by 66 nucleotides Pf1N1B4_3020 and Pf1N1B4_3021 are separated by 356 nucleotides
Pf1N1B4_3018 - L-carnitine dehydratase/bile acid-inducible protein F (EC 2.8.3.16), at 3,393,260 to 3,394,459
_3018
Pf1N1B4_3019 - YaeQ protein, at 3,394,606 to 3,395,148
_3019
Pf1N1B4_3020 - Single-stranded-DNA-specific exonuclease RecJ (EC 3.1.-.-), at 3,395,215 to 3,396,924
_3020
Pf1N1B4_3021 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 3,397,281 to 3,398,387
_3021
Position (kb)
3395
3396
3397 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3394.296 kb on + strand, within Pf1N1B4_3018 at 3394.296 kb on + strand, within Pf1N1B4_3018 at 3394.297 kb on - strand, within Pf1N1B4_3018 at 3394.297 kb on - strand, within Pf1N1B4_3018 at 3394.378 kb on + strand at 3394.378 kb on + strand at 3394.409 kb on - strand at 3394.409 kb on - strand at 3394.502 kb on - strand at 3394.502 kb on - strand at 3394.502 kb on - strand at 3394.506 kb on + strand at 3394.506 kb on + strand at 3394.506 kb on + strand at 3394.506 kb on + strand at 3394.506 kb on + strand at 3394.507 kb on - strand at 3394.507 kb on - strand at 3394.628 kb on - strand at 3394.628 kb on - strand at 3394.628 kb on - strand at 3394.628 kb on - strand at 3394.628 kb on - strand at 3394.670 kb on - strand, within Pf1N1B4_3019 at 3394.745 kb on - strand, within Pf1N1B4_3019 at 3395.150 kb on + strand at 3395.151 kb on - strand at 3395.180 kb on + strand at 3395.180 kb on + strand at 3395.181 kb on - strand at 3395.185 kb on + strand at 3395.185 kb on + strand at 3395.185 kb on + strand at 3395.186 kb on - strand at 3395.186 kb on - strand at 3395.186 kb on - strand at 3395.189 kb on + strand at 3395.189 kb on + strand at 3395.189 kb on + strand at 3395.189 kb on + strand at 3395.189 kb on + strand at 3395.189 kb on + strand at 3395.189 kb on + strand at 3395.190 kb on - strand at 3395.291 kb on - strand at 3395.516 kb on - strand, within Pf1N1B4_3020 at 3395.784 kb on - strand, within Pf1N1B4_3020 at 3395.795 kb on + strand, within Pf1N1B4_3020 at 3395.795 kb on + strand, within Pf1N1B4_3020 at 3395.796 kb on - strand, within Pf1N1B4_3020 at 3395.796 kb on - strand, within Pf1N1B4_3020 at 3395.796 kb on - strand, within Pf1N1B4_3020 at 3395.837 kb on - strand, within Pf1N1B4_3020 at 3395.910 kb on - strand, within Pf1N1B4_3020 at 3396.030 kb on + strand, within Pf1N1B4_3020 at 3396.030 kb on + strand, within Pf1N1B4_3020 at 3396.030 kb on + strand, within Pf1N1B4_3020 at 3396.331 kb on + strand, within Pf1N1B4_3020 at 3396.332 kb on - strand, within Pf1N1B4_3020 at 3396.332 kb on - strand, within Pf1N1B4_3020 at 3396.411 kb on + strand, within Pf1N1B4_3020 at 3396.412 kb on - strand, within Pf1N1B4_3020 at 3396.466 kb on + strand, within Pf1N1B4_3020 at 3396.467 kb on - strand, within Pf1N1B4_3020 at 3396.467 kb on - strand, within Pf1N1B4_3020 at 3396.810 kb on + strand at 3396.810 kb on + strand at 3396.810 kb on + strand at 3396.811 kb on - strand at 3396.850 kb on + strand at 3396.850 kb on + strand at 3396.851 kb on - strand at 3397.143 kb on + strand at 3397.302 kb on + strand at 3397.302 kb on + strand at 3397.302 kb on + strand at 3397.303 kb on - strand at 3397.304 kb on + strand at 3397.304 kb on + strand at 3397.359 kb on + strand at 3397.360 kb on - strand at 3397.360 kb on - strand at 3397.439 kb on - strand, within Pf1N1B4_3021 at 3397.532 kb on + strand, within Pf1N1B4_3021 at 3397.532 kb on + strand, within Pf1N1B4_3021 at 3397.532 kb on + strand, within Pf1N1B4_3021 at 3397.532 kb on + strand, within Pf1N1B4_3021 at 3397.532 kb on + strand, within Pf1N1B4_3021 at 3397.532 kb on + strand, within Pf1N1B4_3021 at 3397.532 kb on + strand, within Pf1N1B4_3021 at 3397.532 kb on + strand, within Pf1N1B4_3021 at 3397.533 kb on - strand, within Pf1N1B4_3021 at 3397.533 kb on - strand, within Pf1N1B4_3021 at 3397.533 kb on - strand, within Pf1N1B4_3021 at 3397.533 kb on - strand, within Pf1N1B4_3021 at 3397.533 kb on - strand, within Pf1N1B4_3021 at 3397.533 kb on - strand, within Pf1N1B4_3021 at 3397.533 kb on - strand at 3397.808 kb on - strand, within Pf1N1B4_3021
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 3,394,296 + Pf1N1B4_3018 0.86 +0.7 3,394,296 + Pf1N1B4_3018 0.86 +1.1 3,394,297 - Pf1N1B4_3018 0.86 -1.0 3,394,297 - Pf1N1B4_3018 0.86 -0.0 3,394,378 + -1.8 3,394,378 + +0.4 3,394,409 - +1.3 3,394,409 - +1.0 3,394,502 - -0.3 3,394,502 - +1.9 3,394,502 - -1.0 3,394,506 + -1.0 3,394,506 + -1.1 3,394,506 + -0.1 3,394,506 + -1.4 3,394,506 + +1.5 3,394,507 - -0.2 3,394,507 - +0.4 3,394,628 - +1.6 3,394,628 - -1.9 3,394,628 - +1.6 3,394,628 - -1.3 3,394,628 - -2.9 3,394,670 - Pf1N1B4_3019 0.12 -2.2 3,394,745 - Pf1N1B4_3019 0.26 +1.3 3,395,150 + +1.2 3,395,151 - +1.2 3,395,180 + +1.7 3,395,180 + +1.5 3,395,181 - -0.5 3,395,185 + +1.7 3,395,185 + +0.8 3,395,185 + -0.6 3,395,186 - +4.0 3,395,186 - -0.4 3,395,186 - -1.6 3,395,189 + +1.6 3,395,189 + -2.7 3,395,189 + -0.5 3,395,189 + +1.5 3,395,189 + -1.9 3,395,189 + -2.5 3,395,189 + -0.3 3,395,190 - -2.7 3,395,291 - -1.6 3,395,516 - Pf1N1B4_3020 0.18 -0.4 3,395,784 - Pf1N1B4_3020 0.33 -1.6 3,395,795 + Pf1N1B4_3020 0.34 +0.3 3,395,795 + Pf1N1B4_3020 0.34 -0.7 3,395,796 - Pf1N1B4_3020 0.34 -0.2 3,395,796 - Pf1N1B4_3020 0.34 -1.6 3,395,796 - Pf1N1B4_3020 0.34 +0.8 3,395,837 - Pf1N1B4_3020 0.36 -1.8 3,395,910 - Pf1N1B4_3020 0.41 +1.2 3,396,030 + Pf1N1B4_3020 0.48 -0.2 3,396,030 + Pf1N1B4_3020 0.48 +1.7 3,396,030 + Pf1N1B4_3020 0.48 +1.6 3,396,331 + Pf1N1B4_3020 0.65 +0.2 3,396,332 - Pf1N1B4_3020 0.65 +0.3 3,396,332 - Pf1N1B4_3020 0.65 +1.3 3,396,411 + Pf1N1B4_3020 0.70 +1.2 3,396,412 - Pf1N1B4_3020 0.70 +1.1 3,396,466 + Pf1N1B4_3020 0.73 -0.1 3,396,467 - Pf1N1B4_3020 0.73 -0.7 3,396,467 - Pf1N1B4_3020 0.73 -0.0 3,396,810 + -1.0 3,396,810 + +0.0 3,396,810 + -0.9 3,396,811 - -3.3 3,396,850 + +1.5 3,396,850 + -0.7 3,396,851 - +0.6 3,397,143 + +0.2 3,397,302 + -1.2 3,397,302 + -0.4 3,397,302 + -0.7 3,397,303 - -0.0 3,397,304 + -0.6 3,397,304 + -1.0 3,397,359 + +1.0 3,397,360 - +0.4 3,397,360 - -1.0 3,397,439 - Pf1N1B4_3021 0.14 +1.3 3,397,532 + Pf1N1B4_3021 0.23 -0.3 3,397,532 + Pf1N1B4_3021 0.23 +1.5 3,397,532 + Pf1N1B4_3021 0.23 -1.1 3,397,532 + Pf1N1B4_3021 0.23 +0.4 3,397,532 + Pf1N1B4_3021 0.23 -2.1 3,397,532 + Pf1N1B4_3021 0.23 +1.9 3,397,532 + Pf1N1B4_3021 0.23 +0.4 3,397,532 + Pf1N1B4_3021 0.23 -1.0 3,397,533 - Pf1N1B4_3021 0.23 +0.6 3,397,533 - Pf1N1B4_3021 0.23 +0.5 3,397,533 - Pf1N1B4_3021 0.23 -0.9 3,397,533 - Pf1N1B4_3021 0.23 +1.1 3,397,533 - Pf1N1B4_3021 0.23 +1.3 3,397,533 - Pf1N1B4_3021 0.23 -0.3 3,397,533 - +2.0 3,397,808 - Pf1N1B4_3021 0.48 -0.6
Or see this region's nucleotide sequence