Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2907 and Pf1N1B4_2908 are separated by 285 nucleotides Pf1N1B4_2908 and Pf1N1B4_5209 are separated by 167 nucleotides Pf1N1B4_5209 and Pf1N1B4_2909 are separated by 116 nucleotides
Pf1N1B4_2907 - Integrase, at 3,262,602 to 3,262,850
_2907
Pf1N1B4_2908 - Integrase, at 3,263,136 to 3,263,828
_2908
Pf1N1B4_5209 - tRNA-Met-CAT, at 3,263,996 to 3,264,069
_5209
Pf1N1B4_2909 - Methyl-accepting chemotaxis protein, at 3,264,186 to 3,266,243
_2909
Position (kb)
3263
3264 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3262.174 kb on + strand at 3262.191 kb on + strand at 3262.207 kb on - strand at 3262.249 kb on + strand at 3262.250 kb on - strand at 3262.399 kb on + strand at 3262.399 kb on + strand at 3262.399 kb on + strand at 3262.400 kb on - strand at 3262.479 kb on + strand at 3262.479 kb on + strand at 3262.479 kb on + strand at 3262.480 kb on - strand at 3262.480 kb on - strand at 3262.483 kb on - strand at 3262.540 kb on + strand at 3262.541 kb on - strand at 3262.541 kb on - strand at 3262.544 kb on + strand at 3262.544 kb on + strand at 3262.544 kb on + strand at 3262.545 kb on - strand at 3262.587 kb on + strand at 3262.588 kb on - strand at 3262.588 kb on - strand at 3262.588 kb on - strand at 3262.588 kb on - strand at 3262.608 kb on - strand at 3262.645 kb on + strand, within Pf1N1B4_2907 at 3262.646 kb on - strand, within Pf1N1B4_2907 at 3262.654 kb on + strand, within Pf1N1B4_2907 at 3262.673 kb on + strand, within Pf1N1B4_2907 at 3262.687 kb on + strand, within Pf1N1B4_2907 at 3262.702 kb on + strand at 3262.702 kb on + strand, within Pf1N1B4_2907 at 3262.702 kb on + strand, within Pf1N1B4_2907 at 3262.702 kb on + strand, within Pf1N1B4_2907 at 3262.702 kb on + strand, within Pf1N1B4_2907 at 3262.702 kb on + strand, within Pf1N1B4_2907 at 3262.702 kb on + strand, within Pf1N1B4_2907 at 3262.702 kb on + strand, within Pf1N1B4_2907 at 3262.703 kb on - strand, within Pf1N1B4_2907 at 3262.703 kb on - strand, within Pf1N1B4_2907 at 3262.703 kb on - strand, within Pf1N1B4_2907 at 3262.703 kb on - strand, within Pf1N1B4_2907 at 3262.703 kb on - strand, within Pf1N1B4_2907 at 3262.703 kb on - strand, within Pf1N1B4_2907 at 3262.703 kb on - strand, within Pf1N1B4_2907 at 3262.703 kb on - strand, within Pf1N1B4_2907 at 3262.734 kb on + strand, within Pf1N1B4_2907 at 3262.734 kb on + strand at 3262.734 kb on + strand, within Pf1N1B4_2907 at 3262.735 kb on - strand, within Pf1N1B4_2907 at 3262.735 kb on - strand, within Pf1N1B4_2907 at 3262.735 kb on - strand, within Pf1N1B4_2907 at 3262.735 kb on - strand, within Pf1N1B4_2907 at 3262.779 kb on + strand, within Pf1N1B4_2907 at 3262.906 kb on - strand at 3262.906 kb on - strand at 3262.933 kb on - strand at 3262.941 kb on - strand at 3262.971 kb on + strand at 3262.971 kb on + strand at 3262.971 kb on + strand at 3262.971 kb on + strand at 3262.971 kb on + strand at 3262.971 kb on + strand at 3262.971 kb on + strand at 3262.972 kb on - strand at 3262.972 kb on - strand at 3262.972 kb on - strand at 3262.972 kb on - strand at 3262.972 kb on - strand at 3262.972 kb on - strand at 3263.056 kb on + strand at 3263.056 kb on + strand at 3263.057 kb on - strand at 3263.057 kb on - strand at 3263.057 kb on - strand at 3263.096 kb on + strand at 3263.097 kb on - strand at 3263.137 kb on + strand at 3263.191 kb on + strand at 3263.192 kb on - strand at 3263.278 kb on + strand, within Pf1N1B4_2908 at 3263.279 kb on - strand, within Pf1N1B4_2908 at 3263.279 kb on - strand at 3263.293 kb on + strand, within Pf1N1B4_2908 at 3263.366 kb on + strand, within Pf1N1B4_2908 at 3263.366 kb on + strand, within Pf1N1B4_2908 at 3263.366 kb on + strand, within Pf1N1B4_2908 at 3263.366 kb on + strand, within Pf1N1B4_2908 at 3263.366 kb on + strand, within Pf1N1B4_2908 at 3263.367 kb on - strand, within Pf1N1B4_2908 at 3263.367 kb on - strand, within Pf1N1B4_2908 at 3263.370 kb on + strand, within Pf1N1B4_2908 at 3263.370 kb on + strand, within Pf1N1B4_2908 at 3263.370 kb on + strand, within Pf1N1B4_2908 at 3263.370 kb on + strand, within Pf1N1B4_2908 at 3263.370 kb on + strand, within Pf1N1B4_2908 at 3263.370 kb on + strand, within Pf1N1B4_2908 at 3263.371 kb on - strand, within Pf1N1B4_2908 at 3263.371 kb on - strand, within Pf1N1B4_2908 at 3263.371 kb on - strand, within Pf1N1B4_2908 at 3263.371 kb on - strand, within Pf1N1B4_2908 at 3263.371 kb on - strand, within Pf1N1B4_2908 at 3263.371 kb on - strand, within Pf1N1B4_2908 at 3263.383 kb on + strand, within Pf1N1B4_2908 at 3263.384 kb on - strand, within Pf1N1B4_2908 at 3263.523 kb on + strand at 3263.523 kb on + strand, within Pf1N1B4_2908 at 3263.523 kb on + strand, within Pf1N1B4_2908 at 3263.524 kb on - strand, within Pf1N1B4_2908 at 3263.524 kb on - strand, within Pf1N1B4_2908 at 3263.524 kb on - strand, within Pf1N1B4_2908 at 3263.524 kb on - strand, within Pf1N1B4_2908 at 3263.524 kb on - strand, within Pf1N1B4_2908 at 3263.524 kb on - strand, within Pf1N1B4_2908 at 3263.541 kb on - strand, within Pf1N1B4_2908 at 3263.541 kb on - strand, within Pf1N1B4_2908 at 3263.541 kb on - strand, within Pf1N1B4_2908 at 3263.589 kb on + strand, within Pf1N1B4_2908 at 3263.589 kb on + strand, within Pf1N1B4_2908 at 3263.678 kb on + strand, within Pf1N1B4_2908 at 3263.678 kb on + strand, within Pf1N1B4_2908 at 3263.678 kb on + strand, within Pf1N1B4_2908 at 3263.678 kb on + strand, within Pf1N1B4_2908 at 3263.680 kb on + strand, within Pf1N1B4_2908 at 3263.680 kb on + strand, within Pf1N1B4_2908 at 3263.681 kb on - strand, within Pf1N1B4_2908 at 3263.708 kb on + strand, within Pf1N1B4_2908 at 3263.709 kb on - strand, within Pf1N1B4_2908 at 3263.725 kb on + strand, within Pf1N1B4_2908 at 3263.725 kb on + strand, within Pf1N1B4_2908 at 3263.726 kb on - strand, within Pf1N1B4_2908 at 3263.742 kb on + strand, within Pf1N1B4_2908 at 3263.742 kb on + strand, within Pf1N1B4_2908 at 3263.742 kb on + strand, within Pf1N1B4_2908 at 3263.743 kb on - strand at 3263.752 kb on + strand, within Pf1N1B4_2908 at 3263.752 kb on + strand, within Pf1N1B4_2908 at 3263.753 kb on - strand, within Pf1N1B4_2908 at 3263.753 kb on - strand, within Pf1N1B4_2908 at 3263.753 kb on - strand at 3263.753 kb on - strand, within Pf1N1B4_2908 at 3263.753 kb on - strand, within Pf1N1B4_2908 at 3263.781 kb on + strand at 3263.782 kb on - strand at 3263.834 kb on + strand at 3263.835 kb on - strand at 3263.835 kb on - strand at 3263.855 kb on - strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.864 kb on + strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.865 kb on - strand at 3263.876 kb on - strand at 3263.915 kb on + strand at 3263.915 kb on + strand at 3263.916 kb on - strand at 3263.916 kb on - strand at 3263.916 kb on - strand at 3263.916 kb on - strand at 3263.919 kb on + strand at 3263.919 kb on + strand at 3263.919 kb on + strand at 3263.962 kb on - strand at 3263.962 kb on - strand at 3263.962 kb on - strand at 3264.125 kb on - strand at 3264.228 kb on - strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.282 kb on + strand at 3264.283 kb on - strand at 3264.283 kb on - strand at 3264.283 kb on - strand at 3264.283 kb on - strand at 3264.283 kb on - strand at 3264.776 kb on + strand, within Pf1N1B4_2909 at 3264.777 kb on - strand, within Pf1N1B4_2909
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 3,262,174 + -0.6 3,262,191 + +2.4 3,262,207 - -0.8 3,262,249 + -0.4 3,262,250 - -1.9 3,262,399 + -1.8 3,262,399 + +0.1 3,262,399 + -2.2 3,262,400 - -1.1 3,262,479 + -0.4 3,262,479 + +0.2 3,262,479 + -2.2 3,262,480 - -0.2 3,262,480 - -0.6 3,262,483 - -1.8 3,262,540 + -0.6 3,262,541 - -2.1 3,262,541 - -0.6 3,262,544 + +0.5 3,262,544 + +0.5 3,262,544 + +0.6 3,262,545 - -1.8 3,262,587 + -1.6 3,262,588 - +1.0 3,262,588 - -0.9 3,262,588 - -1.6 3,262,588 - -0.2 3,262,608 - -0.8 3,262,645 + Pf1N1B4_2907 0.17 -0.1 3,262,646 - Pf1N1B4_2907 0.18 +0.9 3,262,654 + Pf1N1B4_2907 0.21 +1.0 3,262,673 + Pf1N1B4_2907 0.29 +2.4 3,262,687 + Pf1N1B4_2907 0.34 -2.5 3,262,702 + +3.4 3,262,702 + Pf1N1B4_2907 0.40 -0.8 3,262,702 + Pf1N1B4_2907 0.40 -1.6 3,262,702 + Pf1N1B4_2907 0.40 -0.2 3,262,702 + Pf1N1B4_2907 0.40 -1.6 3,262,702 + Pf1N1B4_2907 0.40 -1.3 3,262,702 + Pf1N1B4_2907 0.40 -1.8 3,262,702 + Pf1N1B4_2907 0.40 +0.2 3,262,703 - Pf1N1B4_2907 0.41 +0.8 3,262,703 - Pf1N1B4_2907 0.41 -0.9 3,262,703 - Pf1N1B4_2907 0.41 +0.2 3,262,703 - Pf1N1B4_2907 0.41 -0.5 3,262,703 - Pf1N1B4_2907 0.41 +0.6 3,262,703 - Pf1N1B4_2907 0.41 -1.1 3,262,703 - Pf1N1B4_2907 0.41 -0.9 3,262,703 - Pf1N1B4_2907 0.41 -0.7 3,262,734 + Pf1N1B4_2907 0.53 -0.7 3,262,734 + +0.2 3,262,734 + Pf1N1B4_2907 0.53 +2.1 3,262,735 - Pf1N1B4_2907 0.53 +0.6 3,262,735 - Pf1N1B4_2907 0.53 -1.4 3,262,735 - Pf1N1B4_2907 0.53 -0.3 3,262,735 - Pf1N1B4_2907 0.53 -0.9 3,262,779 + Pf1N1B4_2907 0.71 -0.7 3,262,906 - -0.8 3,262,906 - -0.8 3,262,933 - -0.3 3,262,941 - +1.4 3,262,971 + -0.4 3,262,971 + +0.1 3,262,971 + -0.4 3,262,971 + +0.3 3,262,971 + -2.8 3,262,971 + +1.0 3,262,971 + +0.6 3,262,972 - +0.1 3,262,972 - -1.4 3,262,972 - -1.0 3,262,972 - -0.6 3,262,972 - +0.6 3,262,972 - -1.4 3,263,056 + -1.8 3,263,056 + +0.2 3,263,057 - -0.9 3,263,057 - -1.1 3,263,057 - -1.0 3,263,096 + +0.8 3,263,097 - -0.5 3,263,137 + +2.7 3,263,191 + +0.2 3,263,192 - +1.0 3,263,278 + Pf1N1B4_2908 0.20 -1.7 3,263,279 - Pf1N1B4_2908 0.21 -0.6 3,263,279 - +0.6 3,263,293 + Pf1N1B4_2908 0.23 +1.4 3,263,366 + Pf1N1B4_2908 0.33 +0.6 3,263,366 + Pf1N1B4_2908 0.33 -0.6 3,263,366 + Pf1N1B4_2908 0.33 -0.1 3,263,366 + Pf1N1B4_2908 0.33 +1.0 3,263,366 + Pf1N1B4_2908 0.33 -0.9 3,263,367 - Pf1N1B4_2908 0.33 +1.6 3,263,367 - Pf1N1B4_2908 0.33 +2.1 3,263,370 + Pf1N1B4_2908 0.34 -0.8 3,263,370 + Pf1N1B4_2908 0.34 -0.4 3,263,370 + Pf1N1B4_2908 0.34 -1.1 3,263,370 + Pf1N1B4_2908 0.34 +1.5 3,263,370 + Pf1N1B4_2908 0.34 -0.1 3,263,370 + Pf1N1B4_2908 0.34 -0.9 3,263,371 - Pf1N1B4_2908 0.34 -1.9 3,263,371 - Pf1N1B4_2908 0.34 -1.4 3,263,371 - Pf1N1B4_2908 0.34 +0.8 3,263,371 - Pf1N1B4_2908 0.34 +0.0 3,263,371 - Pf1N1B4_2908 0.34 -0.4 3,263,371 - Pf1N1B4_2908 0.34 -2.2 3,263,383 + Pf1N1B4_2908 0.36 -1.6 3,263,384 - Pf1N1B4_2908 0.36 -0.9 3,263,523 + +0.2 3,263,523 + Pf1N1B4_2908 0.56 -1.1 3,263,523 + Pf1N1B4_2908 0.56 +0.7 3,263,524 - Pf1N1B4_2908 0.56 +2.1 3,263,524 - Pf1N1B4_2908 0.56 +0.8 3,263,524 - Pf1N1B4_2908 0.56 -1.4 3,263,524 - Pf1N1B4_2908 0.56 +1.6 3,263,524 - Pf1N1B4_2908 0.56 +0.1 3,263,524 - Pf1N1B4_2908 0.56 +1.1 3,263,541 - Pf1N1B4_2908 0.58 +0.5 3,263,541 - Pf1N1B4_2908 0.58 +0.9 3,263,541 - Pf1N1B4_2908 0.58 -1.5 3,263,589 + Pf1N1B4_2908 0.65 -0.4 3,263,589 + Pf1N1B4_2908 0.65 +1.6 3,263,678 + Pf1N1B4_2908 0.78 -1.1 3,263,678 + Pf1N1B4_2908 0.78 +3.8 3,263,678 + Pf1N1B4_2908 0.78 -0.2 3,263,678 + Pf1N1B4_2908 0.78 -1.7 3,263,680 + Pf1N1B4_2908 0.78 +0.1 3,263,680 + Pf1N1B4_2908 0.78 -0.3 3,263,681 - Pf1N1B4_2908 0.79 -0.9 3,263,708 + Pf1N1B4_2908 0.83 +1.8 3,263,709 - Pf1N1B4_2908 0.83 -1.8 3,263,725 + Pf1N1B4_2908 0.85 +0.3 3,263,725 + Pf1N1B4_2908 0.85 -0.1 3,263,726 - Pf1N1B4_2908 0.85 +1.2 3,263,742 + Pf1N1B4_2908 0.87 +1.5 3,263,742 + Pf1N1B4_2908 0.87 -1.2 3,263,742 + Pf1N1B4_2908 0.87 -1.1 3,263,743 - +0.4 3,263,752 + Pf1N1B4_2908 0.89 +0.0 3,263,752 + Pf1N1B4_2908 0.89 -1.2 3,263,753 - Pf1N1B4_2908 0.89 -1.5 3,263,753 - Pf1N1B4_2908 0.89 -0.2 3,263,753 - +1.6 3,263,753 - Pf1N1B4_2908 0.89 -0.7 3,263,753 - Pf1N1B4_2908 0.89 -0.9 3,263,781 + -1.2 3,263,782 - +0.9 3,263,834 + -1.7 3,263,835 - -0.1 3,263,835 - -0.9 3,263,855 - -2.4 3,263,864 + -0.2 3,263,864 + -1.4 3,263,864 + -0.1 3,263,864 + -1.6 3,263,864 + -1.9 3,263,864 + -0.5 3,263,864 + +1.1 3,263,864 + -0.9 3,263,864 + -0.1 3,263,864 + -1.6 3,263,864 + -2.3 3,263,864 + +1.9 3,263,864 + +1.2 3,263,865 - -2.2 3,263,865 - -0.6 3,263,865 - -0.5 3,263,865 - -1.6 3,263,865 - -0.3 3,263,865 - -1.8 3,263,865 - +1.4 3,263,865 - -0.1 3,263,865 - -0.3 3,263,865 - +0.8 3,263,865 - -0.2 3,263,865 - +0.1 3,263,876 - -0.8 3,263,915 + +0.2 3,263,915 + -1.3 3,263,916 - -1.7 3,263,916 - -1.6 3,263,916 - -1.4 3,263,916 - -1.4 3,263,919 + +1.0 3,263,919 + -1.4 3,263,919 + +1.9 3,263,962 - +1.4 3,263,962 - +2.2 3,263,962 - -1.5 3,264,125 - -1.1 3,264,228 - -0.5 3,264,282 + +0.8 3,264,282 + -0.4 3,264,282 + +0.1 3,264,282 + -0.9 3,264,282 + -0.6 3,264,282 + -0.9 3,264,282 + +0.2 3,264,282 + -0.6 3,264,283 - +1.5 3,264,283 - -1.4 3,264,283 - +0.2 3,264,283 - +0.3 3,264,283 - -0.9 3,264,776 + Pf1N1B4_2909 0.29 +0.2 3,264,777 - Pf1N1B4_2909 0.29 -0.9
Or see this region's nucleotide sequence