Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2686 and Pf1N1B4_2687 are separated by 176 nucleotides Pf1N1B4_2687 and Pf1N1B4_2688 are separated by 213 nucleotides
Pf1N1B4_2686 - DedA protein, at 3,022,775 to 3,024,091
_2686
Pf1N1B4_2687 - DNA-3-methyladenine glycosylase II (EC 3.2.2.21), at 3,024,268 to 3,024,966
_2687
Pf1N1B4_2688 - Gamma-glutamyl phosphate reductase (EC 1.2.1.41), at 3,025,180 to 3,026,427
_2688
Position (kb)
3024
3025 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3023.516 kb on + strand, within Pf1N1B4_2686 at 3023.516 kb on + strand, within Pf1N1B4_2686 at 3023.619 kb on + strand, within Pf1N1B4_2686 at 3023.619 kb on + strand, within Pf1N1B4_2686 at 3023.706 kb on - strand, within Pf1N1B4_2686 at 3023.772 kb on + strand, within Pf1N1B4_2686 at 3023.772 kb on + strand, within Pf1N1B4_2686 at 3023.772 kb on + strand, within Pf1N1B4_2686 at 3023.772 kb on + strand, within Pf1N1B4_2686 at 3023.853 kb on - strand, within Pf1N1B4_2686 at 3023.860 kb on - strand, within Pf1N1B4_2686 at 3023.900 kb on + strand, within Pf1N1B4_2686 at 3023.963 kb on + strand at 3023.963 kb on + strand at 3023.963 kb on + strand at 3023.964 kb on - strand at 3023.964 kb on - strand at 3023.964 kb on - strand at 3023.964 kb on - strand at 3023.964 kb on - strand at 3023.964 kb on - strand at 3024.074 kb on - strand at 3024.207 kb on + strand at 3024.207 kb on + strand at 3024.208 kb on - strand at 3024.208 kb on - strand at 3024.208 kb on - strand at 3024.208 kb on - strand at 3024.332 kb on + strand at 3024.362 kb on + strand, within Pf1N1B4_2687 at 3024.363 kb on - strand, within Pf1N1B4_2687 at 3024.650 kb on - strand, within Pf1N1B4_2687 at 3024.704 kb on + strand, within Pf1N1B4_2687 at 3024.707 kb on + strand, within Pf1N1B4_2687 at 3024.707 kb on + strand, within Pf1N1B4_2687 at 3024.707 kb on + strand, within Pf1N1B4_2687 at 3024.707 kb on + strand, within Pf1N1B4_2687 at 3024.707 kb on + strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.708 kb on - strand, within Pf1N1B4_2687 at 3024.711 kb on + strand, within Pf1N1B4_2687 at 3024.711 kb on + strand, within Pf1N1B4_2687 at 3024.711 kb on + strand, within Pf1N1B4_2687 at 3024.711 kb on + strand at 3024.712 kb on - strand, within Pf1N1B4_2687 at 3024.712 kb on - strand, within Pf1N1B4_2687 at 3024.712 kb on - strand, within Pf1N1B4_2687 at 3024.712 kb on - strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.713 kb on + strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.714 kb on - strand, within Pf1N1B4_2687 at 3024.744 kb on + strand, within Pf1N1B4_2687 at 3024.794 kb on + strand, within Pf1N1B4_2687 at 3024.862 kb on - strand, within Pf1N1B4_2687 at 3025.012 kb on + strand at 3025.022 kb on - strand at 3025.022 kb on - strand at 3025.102 kb on + strand at 3025.102 kb on + strand at 3025.102 kb on + strand at 3025.102 kb on + strand at 3025.102 kb on + strand at 3025.102 kb on + strand at 3025.102 kb on + strand at 3025.102 kb on + strand at 3025.102 kb on + strand at 3025.102 kb on + strand at 3025.104 kb on + strand at 3025.117 kb on + strand at 3025.135 kb on + strand at 3025.135 kb on + strand at 3025.531 kb on + strand, within Pf1N1B4_2688 at 3025.753 kb on + strand, within Pf1N1B4_2688 at 3025.858 kb on + strand, within Pf1N1B4_2688 at 3025.858 kb on + strand, within Pf1N1B4_2688 at 3025.858 kb on + strand, within Pf1N1B4_2688 at 3025.918 kb on + strand, within Pf1N1B4_2688
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 3,023,516 + Pf1N1B4_2686 0.56 +1.8 3,023,516 + Pf1N1B4_2686 0.56 -1.0 3,023,619 + Pf1N1B4_2686 0.64 +1.4 3,023,619 + Pf1N1B4_2686 0.64 -1.6 3,023,706 - Pf1N1B4_2686 0.71 +0.5 3,023,772 + Pf1N1B4_2686 0.76 -0.2 3,023,772 + Pf1N1B4_2686 0.76 +1.9 3,023,772 + Pf1N1B4_2686 0.76 -2.0 3,023,772 + Pf1N1B4_2686 0.76 -0.5 3,023,853 - Pf1N1B4_2686 0.82 +1.2 3,023,860 - Pf1N1B4_2686 0.82 +0.0 3,023,900 + Pf1N1B4_2686 0.85 -0.8 3,023,963 + -1.5 3,023,963 + +0.6 3,023,963 + -0.2 3,023,964 - +0.2 3,023,964 - -1.4 3,023,964 - +0.4 3,023,964 - -0.4 3,023,964 - -2.1 3,023,964 - -0.8 3,024,074 - +1.4 3,024,207 + -0.3 3,024,207 + +1.4 3,024,208 - -1.0 3,024,208 - -0.5 3,024,208 - +0.5 3,024,208 - -1.8 3,024,332 + -1.4 3,024,362 + Pf1N1B4_2687 0.13 +1.1 3,024,363 - Pf1N1B4_2687 0.14 -2.9 3,024,650 - Pf1N1B4_2687 0.55 -1.4 3,024,704 + Pf1N1B4_2687 0.62 +0.1 3,024,707 + Pf1N1B4_2687 0.63 -1.9 3,024,707 + Pf1N1B4_2687 0.63 -1.7 3,024,707 + Pf1N1B4_2687 0.63 -1.2 3,024,707 + Pf1N1B4_2687 0.63 +0.3 3,024,707 + Pf1N1B4_2687 0.63 +0.8 3,024,708 - Pf1N1B4_2687 0.63 -0.4 3,024,708 - Pf1N1B4_2687 0.63 -1.7 3,024,708 - Pf1N1B4_2687 0.63 -0.6 3,024,708 - Pf1N1B4_2687 0.63 -1.1 3,024,708 - Pf1N1B4_2687 0.63 +0.0 3,024,708 - Pf1N1B4_2687 0.63 -1.7 3,024,708 - Pf1N1B4_2687 0.63 -0.0 3,024,708 - Pf1N1B4_2687 0.63 -1.5 3,024,708 - Pf1N1B4_2687 0.63 -0.8 3,024,708 - Pf1N1B4_2687 0.63 -2.1 3,024,711 + Pf1N1B4_2687 0.63 -2.3 3,024,711 + Pf1N1B4_2687 0.63 -0.8 3,024,711 + Pf1N1B4_2687 0.63 -1.1 3,024,711 + -0.2 3,024,712 - Pf1N1B4_2687 0.64 -1.1 3,024,712 - Pf1N1B4_2687 0.64 -1.0 3,024,712 - Pf1N1B4_2687 0.64 -0.2 3,024,712 - Pf1N1B4_2687 0.64 -2.1 3,024,713 + Pf1N1B4_2687 0.64 -2.4 3,024,713 + Pf1N1B4_2687 0.64 +0.2 3,024,713 + Pf1N1B4_2687 0.64 -0.8 3,024,713 + Pf1N1B4_2687 0.64 -0.1 3,024,713 + Pf1N1B4_2687 0.64 +1.1 3,024,713 + Pf1N1B4_2687 0.64 +1.0 3,024,713 + Pf1N1B4_2687 0.64 -0.6 3,024,713 + Pf1N1B4_2687 0.64 -2.4 3,024,713 + Pf1N1B4_2687 0.64 -0.3 3,024,713 + Pf1N1B4_2687 0.64 -1.9 3,024,713 + Pf1N1B4_2687 0.64 -2.0 3,024,713 + Pf1N1B4_2687 0.64 -1.4 3,024,713 + Pf1N1B4_2687 0.64 -0.6 3,024,714 - Pf1N1B4_2687 0.64 -0.3 3,024,714 - +1.1 3,024,714 - Pf1N1B4_2687 0.64 -1.0 3,024,714 - -0.4 3,024,714 - Pf1N1B4_2687 0.64 -0.4 3,024,714 - Pf1N1B4_2687 0.64 -0.8 3,024,714 - Pf1N1B4_2687 0.64 +0.8 3,024,714 - Pf1N1B4_2687 0.64 -1.4 3,024,714 - Pf1N1B4_2687 0.64 -2.0 3,024,714 - Pf1N1B4_2687 0.64 -1.0 3,024,714 - Pf1N1B4_2687 0.64 -1.4 3,024,714 - Pf1N1B4_2687 0.64 -1.1 3,024,714 - Pf1N1B4_2687 0.64 -1.6 3,024,714 - Pf1N1B4_2687 0.64 +0.1 3,024,714 - Pf1N1B4_2687 0.64 -0.4 3,024,714 - Pf1N1B4_2687 0.64 -1.9 3,024,744 + Pf1N1B4_2687 0.68 -0.3 3,024,794 + Pf1N1B4_2687 0.75 -2.0 3,024,862 - Pf1N1B4_2687 0.85 +2.2 3,025,012 + -0.1 3,025,022 - -0.8 3,025,022 - -0.8 3,025,102 + -1.7 3,025,102 + -0.8 3,025,102 + +0.8 3,025,102 + -3.2 3,025,102 + -2.0 3,025,102 + +0.6 3,025,102 + +1.5 3,025,102 + +0.0 3,025,102 + -2.5 3,025,102 + +0.0 3,025,104 + -0.4 3,025,117 + -2.0 3,025,135 + -0.4 3,025,135 + -0.1 3,025,531 + Pf1N1B4_2688 0.28 -1.1 3,025,753 + Pf1N1B4_2688 0.46 -0.6 3,025,858 + Pf1N1B4_2688 0.54 +1.2 3,025,858 + Pf1N1B4_2688 0.54 -1.1 3,025,858 + Pf1N1B4_2688 0.54 -1.1 3,025,918 + Pf1N1B4_2688 0.59 +0.5
Or see this region's nucleotide sequence