Experiment: Minimal media pH9
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2503 and Pf1N1B4_2504 are separated by 38 nucleotides Pf1N1B4_2504 and Pf1N1B4_2505 are separated by 12 nucleotides
Pf1N1B4_2503 - Coenzyme PQQ synthesis protein F, at 2,838,560 to 2,840,998
_2503
Pf1N1B4_2504 - 5-aminopentanamidase (EC 3.5.1.30) (from data) , at 2,841,037 to 2,841,831
_2504
Pf1N1B4_2505 - Lysine 2-monooxygenase (EC 1.13.12.2) (from data) , at 2,841,844 to 2,843,526
_2505
Position (kb)
2841
2842 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2840.278 kb on - strand, within Pf1N1B4_2503 at 2840.278 kb on - strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.281 kb on + strand, within Pf1N1B4_2503 at 2840.282 kb on - strand, within Pf1N1B4_2503 at 2840.282 kb on - strand, within Pf1N1B4_2503 at 2840.282 kb on - strand, within Pf1N1B4_2503 at 2840.282 kb on - strand, within Pf1N1B4_2503 at 2840.282 kb on - strand, within Pf1N1B4_2503 at 2840.298 kb on - strand, within Pf1N1B4_2503 at 2840.298 kb on - strand, within Pf1N1B4_2503 at 2840.298 kb on - strand, within Pf1N1B4_2503 at 2840.298 kb on - strand, within Pf1N1B4_2503 at 2840.344 kb on + strand, within Pf1N1B4_2503 at 2840.344 kb on + strand, within Pf1N1B4_2503 at 2840.344 kb on + strand, within Pf1N1B4_2503 at 2840.344 kb on + strand, within Pf1N1B4_2503 at 2840.344 kb on + strand, within Pf1N1B4_2503 at 2840.344 kb on + strand, within Pf1N1B4_2503 at 2840.344 kb on + strand, within Pf1N1B4_2503 at 2840.345 kb on - strand, within Pf1N1B4_2503 at 2840.345 kb on - strand, within Pf1N1B4_2503 at 2840.443 kb on - strand, within Pf1N1B4_2503 at 2840.547 kb on + strand, within Pf1N1B4_2503 at 2840.547 kb on + strand at 2840.547 kb on + strand, within Pf1N1B4_2503 at 2840.548 kb on - strand, within Pf1N1B4_2503 at 2840.644 kb on + strand, within Pf1N1B4_2503 at 2840.728 kb on + strand, within Pf1N1B4_2503 at 2840.728 kb on + strand, within Pf1N1B4_2503 at 2840.729 kb on - strand, within Pf1N1B4_2503 at 2840.775 kb on + strand at 2840.775 kb on + strand at 2840.776 kb on - strand at 2840.776 kb on - strand at 2840.776 kb on - strand at 2841.025 kb on - strand at 2841.060 kb on - strand at 2841.061 kb on + strand at 2841.062 kb on - strand at 2841.062 kb on - strand at 2841.062 kb on - strand at 2841.062 kb on - strand at 2841.087 kb on - strand at 2841.212 kb on + strand, within Pf1N1B4_2504 at 2841.239 kb on + strand, within Pf1N1B4_2504 at 2841.239 kb on + strand, within Pf1N1B4_2504 at 2841.240 kb on - strand, within Pf1N1B4_2504 at 2841.240 kb on - strand, within Pf1N1B4_2504 at 2841.240 kb on - strand, within Pf1N1B4_2504 at 2841.240 kb on - strand, within Pf1N1B4_2504 at 2841.248 kb on + strand, within Pf1N1B4_2504 at 2841.249 kb on - strand, within Pf1N1B4_2504 at 2841.257 kb on + strand, within Pf1N1B4_2504 at 2841.257 kb on + strand, within Pf1N1B4_2504 at 2841.258 kb on - strand at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.258 kb on - strand, within Pf1N1B4_2504 at 2841.509 kb on + strand, within Pf1N1B4_2504 at 2841.548 kb on - strand, within Pf1N1B4_2504 at 2841.557 kb on + strand, within Pf1N1B4_2504 at 2841.557 kb on + strand, within Pf1N1B4_2504 at 2841.557 kb on + strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.558 kb on - strand, within Pf1N1B4_2504 at 2841.593 kb on + strand, within Pf1N1B4_2504 at 2841.594 kb on - strand, within Pf1N1B4_2504 at 2841.594 kb on - strand, within Pf1N1B4_2504 at 2841.699 kb on - strand, within Pf1N1B4_2504 at 2841.699 kb on - strand, within Pf1N1B4_2504 at 2841.781 kb on - strand at 2841.781 kb on - strand at 2841.845 kb on + strand at 2841.845 kb on + strand at 2842.064 kb on + strand, within Pf1N1B4_2505 at 2842.079 kb on + strand, within Pf1N1B4_2505 at 2842.280 kb on + strand, within Pf1N1B4_2505 at 2842.280 kb on + strand, within Pf1N1B4_2505 at 2842.281 kb on - strand, within Pf1N1B4_2505 at 2842.329 kb on - strand, within Pf1N1B4_2505 at 2842.329 kb on - strand, within Pf1N1B4_2505 at 2842.329 kb on - strand, within Pf1N1B4_2505 at 2842.448 kb on + strand, within Pf1N1B4_2505 at 2842.449 kb on - strand, within Pf1N1B4_2505 at 2842.547 kb on - strand, within Pf1N1B4_2505 at 2842.574 kb on + strand at 2842.575 kb on - strand, within Pf1N1B4_2505 at 2842.575 kb on - strand, within Pf1N1B4_2505 at 2842.591 kb on - strand, within Pf1N1B4_2505 at 2842.591 kb on - strand, within Pf1N1B4_2505 at 2842.591 kb on - strand, within Pf1N1B4_2505 at 2842.591 kb on - strand, within Pf1N1B4_2505 at 2842.744 kb on + strand, within Pf1N1B4_2505
Per-strain Table
Position Strand Gene LocusTag Fraction Minimal media pH9 remove 2,840,278 - Pf1N1B4_2503 0.70 +1.4 2,840,278 - Pf1N1B4_2503 0.70 +0.3 2,840,281 + Pf1N1B4_2503 0.71 +0.6 2,840,281 + Pf1N1B4_2503 0.71 +0.1 2,840,281 + Pf1N1B4_2503 0.71 +0.9 2,840,281 + Pf1N1B4_2503 0.71 -0.3 2,840,281 + Pf1N1B4_2503 0.71 +1.5 2,840,281 + Pf1N1B4_2503 0.71 +1.3 2,840,281 + Pf1N1B4_2503 0.71 -1.6 2,840,281 + Pf1N1B4_2503 0.71 +1.4 2,840,281 + Pf1N1B4_2503 0.71 -2.7 2,840,281 + Pf1N1B4_2503 0.71 -1.7 2,840,281 + Pf1N1B4_2503 0.71 +2.2 2,840,281 + Pf1N1B4_2503 0.71 +0.5 2,840,281 + Pf1N1B4_2503 0.71 +1.2 2,840,281 + Pf1N1B4_2503 0.71 +0.2 2,840,282 - Pf1N1B4_2503 0.71 -0.2 2,840,282 - Pf1N1B4_2503 0.71 -0.4 2,840,282 - Pf1N1B4_2503 0.71 +0.8 2,840,282 - Pf1N1B4_2503 0.71 -0.5 2,840,282 - Pf1N1B4_2503 0.71 +1.6 2,840,298 - Pf1N1B4_2503 0.71 -2.3 2,840,298 - Pf1N1B4_2503 0.71 -0.0 2,840,298 - Pf1N1B4_2503 0.71 -0.4 2,840,298 - Pf1N1B4_2503 0.71 -2.0 2,840,344 + Pf1N1B4_2503 0.73 +1.6 2,840,344 + Pf1N1B4_2503 0.73 +0.4 2,840,344 + Pf1N1B4_2503 0.73 +1.9 2,840,344 + Pf1N1B4_2503 0.73 -0.4 2,840,344 + Pf1N1B4_2503 0.73 -0.1 2,840,344 + Pf1N1B4_2503 0.73 -1.8 2,840,344 + Pf1N1B4_2503 0.73 +0.5 2,840,345 - Pf1N1B4_2503 0.73 +1.9 2,840,345 - Pf1N1B4_2503 0.73 +0.5 2,840,443 - Pf1N1B4_2503 0.77 -0.5 2,840,547 + Pf1N1B4_2503 0.81 -0.8 2,840,547 + +0.8 2,840,547 + Pf1N1B4_2503 0.81 -0.2 2,840,548 - Pf1N1B4_2503 0.82 -0.9 2,840,644 + Pf1N1B4_2503 0.85 +0.5 2,840,728 + Pf1N1B4_2503 0.89 -2.0 2,840,728 + Pf1N1B4_2503 0.89 -1.2 2,840,729 - Pf1N1B4_2503 0.89 -2.2 2,840,775 + +0.3 2,840,775 + +2.5 2,840,776 - +0.6 2,840,776 - -1.7 2,840,776 - +0.3 2,841,025 - +0.4 2,841,060 - -0.3 2,841,061 + -2.5 2,841,062 - +1.2 2,841,062 - +1.4 2,841,062 - -2.5 2,841,062 - +0.1 2,841,087 - -1.1 2,841,212 + Pf1N1B4_2504 0.22 -1.6 2,841,239 + Pf1N1B4_2504 0.25 -1.2 2,841,239 + Pf1N1B4_2504 0.25 +0.4 2,841,240 - Pf1N1B4_2504 0.26 -0.9 2,841,240 - Pf1N1B4_2504 0.26 +0.1 2,841,240 - Pf1N1B4_2504 0.26 -2.2 2,841,240 - Pf1N1B4_2504 0.26 +0.1 2,841,248 + Pf1N1B4_2504 0.27 -1.1 2,841,249 - Pf1N1B4_2504 0.27 -0.8 2,841,257 + Pf1N1B4_2504 0.28 +0.4 2,841,257 + Pf1N1B4_2504 0.28 -0.8 2,841,258 - +2.4 2,841,258 - Pf1N1B4_2504 0.28 -0.0 2,841,258 - Pf1N1B4_2504 0.28 -1.7 2,841,258 - Pf1N1B4_2504 0.28 +0.9 2,841,258 - Pf1N1B4_2504 0.28 +0.1 2,841,258 - Pf1N1B4_2504 0.28 -2.0 2,841,258 - Pf1N1B4_2504 0.28 -1.8 2,841,258 - Pf1N1B4_2504 0.28 -1.2 2,841,509 + Pf1N1B4_2504 0.59 -0.9 2,841,548 - Pf1N1B4_2504 0.64 -0.1 2,841,557 + Pf1N1B4_2504 0.65 -0.8 2,841,557 + Pf1N1B4_2504 0.65 -0.2 2,841,557 + Pf1N1B4_2504 0.65 -2.8 2,841,558 - Pf1N1B4_2504 0.66 -0.4 2,841,558 - Pf1N1B4_2504 0.66 -0.7 2,841,558 - Pf1N1B4_2504 0.66 +0.5 2,841,558 - Pf1N1B4_2504 0.66 -0.3 2,841,558 - Pf1N1B4_2504 0.66 -0.8 2,841,558 - Pf1N1B4_2504 0.66 -2.1 2,841,558 - Pf1N1B4_2504 0.66 +1.4 2,841,558 - Pf1N1B4_2504 0.66 -1.1 2,841,593 + Pf1N1B4_2504 0.70 +0.1 2,841,594 - Pf1N1B4_2504 0.70 -0.7 2,841,594 - Pf1N1B4_2504 0.70 -1.4 2,841,699 - Pf1N1B4_2504 0.83 -2.0 2,841,699 - Pf1N1B4_2504 0.83 -1.2 2,841,781 - -1.7 2,841,781 - -2.9 2,841,845 + -0.6 2,841,845 + +0.5 2,842,064 + Pf1N1B4_2505 0.13 -0.4 2,842,079 + Pf1N1B4_2505 0.14 -1.1 2,842,280 + Pf1N1B4_2505 0.26 -0.3 2,842,280 + Pf1N1B4_2505 0.26 -2.4 2,842,281 - Pf1N1B4_2505 0.26 -0.9 2,842,329 - Pf1N1B4_2505 0.29 +0.1 2,842,329 - Pf1N1B4_2505 0.29 -1.2 2,842,329 - Pf1N1B4_2505 0.29 -1.0 2,842,448 + Pf1N1B4_2505 0.36 +1.0 2,842,449 - Pf1N1B4_2505 0.36 +0.8 2,842,547 - Pf1N1B4_2505 0.42 +0.2 2,842,574 + +1.1 2,842,575 - Pf1N1B4_2505 0.43 -1.1 2,842,575 - Pf1N1B4_2505 0.43 +1.2 2,842,591 - Pf1N1B4_2505 0.44 +2.4 2,842,591 - Pf1N1B4_2505 0.44 +0.4 2,842,591 - Pf1N1B4_2505 0.44 -0.6 2,842,591 - Pf1N1B4_2505 0.44 -1.2 2,842,744 + Pf1N1B4_2505 0.53 -0.9
Or see this region's nucleotide sequence