Experiment: Minimal media pH9 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt Pf1N1B4_2452 and Pf1N1B4_2453 overlap by 11 nucleotides Pf1N1B4_2453 and Pf1N1B4_2454 are separated by 192 nucleotides Pf1N1B4_2454 and Pf1N1B4_2455 are separated by 23 nucleotides 
        Pf1N1B4_2452 - Membrane protein, putative, at 2,782,636 to 2,783,352 
        _2452 
         
        
        Pf1N1B4_2453 - hypothetical protein, at 2,783,342 to 2,783,473 
        _2453 
         
        
        Pf1N1B4_2454 - Serine hydroxymethyltransferase (EC 2.1.2.1) (from data) , at 2,783,666 to 2,784,919 
        _2454 
         
        
        Pf1N1B4_2455 - Sarcosine oxidase beta subunit (EC 1.5.3.1), at 2,784,943 to 2,786,193 
        _2455 
         Position (kb) 2783 
2784 
2785 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 at 2782.717 kb on + strand, within Pf1N1B4_2452 at 2782.717 kb on + strand, within Pf1N1B4_2452 at 2782.718 kb on - strand, within Pf1N1B4_2452 at 2782.744 kb on + strand, within Pf1N1B4_2452 at 2782.744 kb on + strand, within Pf1N1B4_2452 at 2782.744 kb on + strand, within Pf1N1B4_2452 at 2782.744 kb on + strand, within Pf1N1B4_2452 at 2782.744 kb on + strand, within Pf1N1B4_2452 at 2782.745 kb on - strand, within Pf1N1B4_2452 at 2782.745 kb on - strand, within Pf1N1B4_2452 at 2782.745 kb on - strand, within Pf1N1B4_2452 at 2782.745 kb on - strand, within Pf1N1B4_2452 at 2782.863 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.894 kb on + strand, within Pf1N1B4_2452 at 2782.895 kb on - strand, within Pf1N1B4_2452 at 2782.895 kb on - strand, within Pf1N1B4_2452 at 2782.895 kb on - strand, within Pf1N1B4_2452 at 2782.916 kb on + strand, within Pf1N1B4_2452 at 2782.916 kb on + strand, within Pf1N1B4_2452 at 2782.917 kb on - strand, within Pf1N1B4_2452 at 2782.917 kb on - strand, within Pf1N1B4_2452 at 2783.091 kb on + strand, within Pf1N1B4_2452 at 2783.104 kb on + strand, within Pf1N1B4_2452 at 2783.162 kb on - strand, within Pf1N1B4_2452 at 2783.162 kb on - strand, within Pf1N1B4_2452 at 2783.164 kb on + strand, within Pf1N1B4_2452 at 2783.164 kb on + strand, within Pf1N1B4_2452 at 2783.164 kb on + strand, within Pf1N1B4_2452 at 2783.164 kb on + strand, within Pf1N1B4_2452 at 2783.293 kb on + strand at 2783.293 kb on + strand at 2783.302 kb on + strand at 2783.365 kb on + strand, within Pf1N1B4_2453 at 2783.365 kb on + strand, within Pf1N1B4_2453 at 2783.366 kb on - strand, within Pf1N1B4_2453 at 2783.583 kb on + strand at 2783.583 kb on + strand at 2783.584 kb on - strand at 2783.643 kb on + strand at 2783.643 kb on + strand at 2783.643 kb on + strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.644 kb on - strand at 2783.777 kb on + strand at 2783.777 kb on + strand at 2783.777 kb on + strand at 2783.777 kb on + strand at 2783.777 kb on + strand at 2783.778 kb on - strand at 2783.778 kb on - strand at 2783.778 kb on - strand at 2783.778 kb on - strand at 2783.778 kb on - strand at 2783.828 kb on + strand, within Pf1N1B4_2454 at 2783.829 kb on - strand, within Pf1N1B4_2454 at 2783.858 kb on + strand at 2783.859 kb on - strand, within Pf1N1B4_2454 at 2783.934 kb on - strand, within Pf1N1B4_2454 at 2783.981 kb on + strand, within Pf1N1B4_2454 at 2783.982 kb on - strand, within Pf1N1B4_2454 at 2784.083 kb on + strand, within Pf1N1B4_2454 at 2784.083 kb on + strand, within Pf1N1B4_2454 at 2784.084 kb on - strand, within Pf1N1B4_2454 at 2784.084 kb on - strand, within Pf1N1B4_2454 at 2784.084 kb on - strand, within Pf1N1B4_2454 at 2784.099 kb on - strand, within Pf1N1B4_2454 at 2784.324 kb on - strand, within Pf1N1B4_2454 at 2784.525 kb on - strand, within Pf1N1B4_2454 at 2784.572 kb on - strand, within Pf1N1B4_2454 at 2784.591 kb on + strand, within Pf1N1B4_2454 at 2784.591 kb on + strand, within Pf1N1B4_2454 at 2784.591 kb on + strand, within Pf1N1B4_2454 at 2784.591 kb on + strand, within Pf1N1B4_2454 at 2784.591 kb on + strand, within Pf1N1B4_2454 at 2784.592 kb on - strand, within Pf1N1B4_2454 at 2784.592 kb on - strand, within Pf1N1B4_2454 at 2784.592 kb on - strand, within Pf1N1B4_2454 at 2784.934 kb on + strand at 2784.935 kb on - strand at 2784.943 kb on - strand at 2784.943 kb on - strand at 2785.039 kb on + strand at 2785.039 kb on + strand at 2785.039 kb on + strand at 2785.040 kb on - strand at 2785.091 kb on - strand, within Pf1N1B4_2455 at 2785.192 kb on + strand, within Pf1N1B4_2455 at 2785.192 kb on + strand, within Pf1N1B4_2455 at 2785.193 kb on - strand, within Pf1N1B4_2455 at 2785.193 kb on - strand at 2785.267 kb on + strand, within Pf1N1B4_2455 at 2785.267 kb on + strand, within Pf1N1B4_2455 at 2785.268 kb on - strand, within Pf1N1B4_2455 at 2785.268 kb on - strand, within Pf1N1B4_2455 at 2785.268 kb on - strand, within Pf1N1B4_2455 at 2785.789 kb on + strand, within Pf1N1B4_2455 at 2785.789 kb on + strand, within Pf1N1B4_2455 at 2785.790 kb on - strand, within Pf1N1B4_2455  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction Minimal media pH9 remove 2,782,717 +  Pf1N1B4_2452 0.11  +0.2 2,782,717 +  Pf1N1B4_2452 0.11  +0.9 2,782,718 -  Pf1N1B4_2452 0.11  +1.1 2,782,744 +  Pf1N1B4_2452 0.15  -0.2 2,782,744 +  Pf1N1B4_2452 0.15  +2.6 2,782,744 +  Pf1N1B4_2452 0.15  +1.2 2,782,744 +  Pf1N1B4_2452 0.15  +1.4 2,782,744 +  Pf1N1B4_2452 0.15  -2.0 2,782,745 -  Pf1N1B4_2452 0.15  -2.3 2,782,745 -  Pf1N1B4_2452 0.15  +0.6 2,782,745 -  Pf1N1B4_2452 0.15  +0.1 2,782,745 -  Pf1N1B4_2452 0.15  -0.1 2,782,863 +  Pf1N1B4_2452 0.32  +0.2 2,782,894 +  Pf1N1B4_2452 0.36  +0.6 2,782,894 +  Pf1N1B4_2452 0.36  -1.3 2,782,894 +  Pf1N1B4_2452 0.36  +2.0 2,782,894 +  Pf1N1B4_2452 0.36  -2.8 2,782,894 +  Pf1N1B4_2452 0.36  -0.1 2,782,894 +  Pf1N1B4_2452 0.36  -0.2 2,782,894 +  Pf1N1B4_2452 0.36  +0.4 2,782,894 +  Pf1N1B4_2452 0.36  -0.4 2,782,894 +  Pf1N1B4_2452 0.36  +1.7 2,782,895 -  Pf1N1B4_2452 0.36  -1.5 2,782,895 -  Pf1N1B4_2452 0.36  -1.3 2,782,895 -  Pf1N1B4_2452 0.36  +0.7 2,782,916 +  Pf1N1B4_2452 0.39  +0.8 2,782,916 +  Pf1N1B4_2452 0.39  -1.0 2,782,917 -  Pf1N1B4_2452 0.39  -1.3 2,782,917 -  Pf1N1B4_2452 0.39  -1.2 2,783,091 +  Pf1N1B4_2452 0.63  -1.2 2,783,104 +  Pf1N1B4_2452 0.65  +2.4 2,783,162 -  Pf1N1B4_2452 0.73  -1.4 2,783,162 -  Pf1N1B4_2452 0.73  -0.4 2,783,164 +  Pf1N1B4_2452 0.74  -0.4 2,783,164 +  Pf1N1B4_2452 0.74  -2.1 2,783,164 +  Pf1N1B4_2452 0.74  -1.7 2,783,164 +  Pf1N1B4_2452 0.74  -0.0 2,783,293 +  -2.7 2,783,293 +  +0.4 2,783,302 +  -0.5 2,783,365 +  Pf1N1B4_2453 0.17  -2.2 2,783,365 +  Pf1N1B4_2453 0.17  -0.9 2,783,366 -  Pf1N1B4_2453 0.18  -1.4 2,783,583 +  -1.2 2,783,583 +  +0.6 2,783,584 -  +0.7 2,783,643 +  +2.3 2,783,643 +  +1.4 2,783,643 +  -2.0 2,783,644 -  -1.2 2,783,644 -  +1.2 2,783,644 -  -2.6 2,783,644 -  -1.5 2,783,644 -  +2.4 2,783,644 -  +1.6 2,783,644 -  +0.9 2,783,644 -  -0.7 2,783,777 +  -1.4 2,783,777 +  +0.6 2,783,777 +  -0.0 2,783,777 +  -1.5 2,783,777 +  -0.6 2,783,778 -  +0.5 2,783,778 -  +1.7 2,783,778 -  -2.0 2,783,778 -  +0.9 2,783,778 -  +0.3 2,783,828 +  Pf1N1B4_2454 0.13  -0.9 2,783,829 -  Pf1N1B4_2454 0.13  -0.2 2,783,858 +  -0.6 2,783,859 -  Pf1N1B4_2454 0.15  -2.4 2,783,934 -  Pf1N1B4_2454 0.21  +0.5 2,783,981 +  Pf1N1B4_2454 0.25  -0.3 2,783,982 -  Pf1N1B4_2454 0.25  +0.0 2,784,083 +  Pf1N1B4_2454 0.33  -3.0 2,784,083 +  Pf1N1B4_2454 0.33  -0.0 2,784,084 -  Pf1N1B4_2454 0.33  +0.2 2,784,084 -  Pf1N1B4_2454 0.33  +0.7 2,784,084 -  Pf1N1B4_2454 0.33  +0.2 2,784,099 -  Pf1N1B4_2454 0.35  +1.6 2,784,324 -  Pf1N1B4_2454 0.52  -0.9 2,784,525 -  Pf1N1B4_2454 0.69  -2.2 2,784,572 -  Pf1N1B4_2454 0.72  -0.9 2,784,591 +  Pf1N1B4_2454 0.74  -0.2 2,784,591 +  Pf1N1B4_2454 0.74  +0.0 2,784,591 +  Pf1N1B4_2454 0.74  -1.3 2,784,591 +  Pf1N1B4_2454 0.74  -1.8 2,784,591 +  Pf1N1B4_2454 0.74  -1.2 2,784,592 -  Pf1N1B4_2454 0.74  +0.8 2,784,592 -  Pf1N1B4_2454 0.74  -0.1 2,784,592 -  Pf1N1B4_2454 0.74  +1.9 2,784,934 +  -0.0 2,784,935 -  -1.0 2,784,943 -  +0.3 2,784,943 -  -1.3 2,785,039 +  -0.9 2,785,039 +  -0.3 2,785,039 +  +0.8 2,785,040 -  -0.4 2,785,091 -  Pf1N1B4_2455 0.12  -0.3 2,785,192 +  Pf1N1B4_2455 0.20  -0.2 2,785,192 +  Pf1N1B4_2455 0.20  -1.4 2,785,193 -  Pf1N1B4_2455 0.20  -0.3 2,785,193 -  +2.4 2,785,267 +  Pf1N1B4_2455 0.26  -0.2 2,785,267 +  Pf1N1B4_2455 0.26  -1.5 2,785,268 -  Pf1N1B4_2455 0.26  -0.0 2,785,268 -  Pf1N1B4_2455 0.26  +1.8 2,785,268 -  Pf1N1B4_2455 0.26  +1.2 2,785,789 +  Pf1N1B4_2455 0.68  -0.9 2,785,789 +  Pf1N1B4_2455 0.68  -1.7 2,785,790 -  Pf1N1B4_2455 0.68  -0.7 
Or see this region's nucleotide sequence